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All Journal Indonesia Medicus Veterinus Media Kedokteran Hewan Jurnal Medik Veteriner Jurnal Medika Veterinaria
Sewoyo, Palagan Senopati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Profil Bobot Badan dan Tingkat Mortalitas Tikus Jantan Galur Sprague Dawley Selama Pembentukan Fibrosarkoma yang Dipicu oleh Benzo(a)piren Sewoyo, Palagan Senopati; Adi, Anak Agung Ayu Mirah; Winaya, Ida Bagus Oka
Indonesia Medicus Veterinus Vol 10 (1) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.1.1

Abstract

Benzo(a)piren (BaP) merupakan satu di antara beberapa contoh polisiklik aromatik hidrokarbon yang berasal dari hasil sisa pembakaran tidak sempurna bahan organik. Senyawa BaP seringkali digunakan pada penelitian untuk menginduksi fibrosarkoma. Secara umum pasien yang mengalami kanker akan mengalami penurunan bobot badan. Penelitian ini bertujuan untuk mengetahui profil bobot badan, waktu yang dibutuhkan untuk menimbulkan fibrosarkoma serta tingkat mortalitas tikus jantan galur Sprague Dawley setelah diinjeksi dengan BaP. Penelitian ini digunakan 18 ekor tikus dengan dua perlakuan. Tikus pada perlakuan 0 (P0) tidak diberi perlakuan, sedangkan tikus pada perlakuan I (PI) diinjeksi dengan BaP 0,3% yang dilarutkan dalam oleum olivarum 0,1 mL sebanyak sepuluh kali yang diberikan bertahap dengan interval dua hari melalui injeksi subkutan. Tikus P0 dan PI berjumlah masing-masing enam dan 12 ekor. Larutan BaP dibuat dengan cara dilarutkan dalam oleum olivarum, dicampur dan diaduk sampai homogen. Bobot badan tikus ditimbang pada awal penelitian kemudian dilakukan secara rutin setiap seminggu sekali selama 19 minggu. Diawal penelitian rerata bobot badan tikus pada P0 dan PI secara berurutan adalah 121,43 ± 7,04 g dan 131,49 ± 16,31 g. Rerata bobot badan tikus pada P0 dan PI dari minggu pertama hingga ke-19 adalah 178,53 ± 29,97 g dan 159,20 ± 14,24 g. Waktu yang dibutuhkan untuk memicu fibrosarkoma adalah 85,5 ± 17,6 hari. Tingkat mortalitas pada perlakuan P0 adalah 0% dan pada perlakuan PI 8,33%. Hasil penelitian ini dapat disimpulkan bahwa pemberian BaP secara signifikan menurunkan profil bobot bobot badan tikus dan memiliki tingkat mortalitas mencapai 8,33%.
Mammary Tumors in Dogs, Recent Perspectives and Antiangiogenesis as a Therapeutic Strategy: Literature Study Sewoyo, Palagan Senopati; Adi, Anak Agung Ayu Mirah; Winaya, Ida Bagus Oka; Wirata, I Wayan
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.271-287

Abstract

A particular type of tumor that is frequently detected in female dogs who are sexually active is a mammary tumor. Neoplasia results from DNA-based alterations in cell cycle regulating genes. The mammary gland is prone to the formation of tumors due to its dynamic structure. The development of this tumor is supported by numerous variables. It has been recently discovered that there is substantial evidence linking the BRCA2 gene to the process of cancer. Standard examination techniques, such as fine needle aspiration, histopathology, and immunohistochemistry, are used along with ancillary tests to determine the tumor type and degree of malignancy. The primary treatment option for malignant tumors is surgical resection followed by adjuvant chemotherapy; benign tumors necessitate surgical resection as well. Adjuvant therapy options include hormone therapy and non-steroidal anti-inflammatory medications. Tumor tissue undergoes angiogenesis as it grows and develops to accommodate the abundant supply of nutrients. Therefore, angiogenesis-inhibiting therapies can be utilized to halt the growth of tumor cells. A number of antiangiogenic medications are now being studied in clinical settings on humans, and several more are undergoing trials on animals. In addition to pharmaceuticals, viruses may be used as a therapeutic to block tumor angiogenesis.
Sebaceous Adenoma in a Geriatric Poodle Dog: A Case Report Winaya, Ida Bagus Oka; Adi, Anak Agung Ayu Mirah; Sudimartini, Luh Made; Merdana, I Made; Sudipa, Putu Henrywaesa; Pemayun, I Gusti Agung Gde Putra; Sewoyo, Palagan Senopati
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.413-419

Abstract

A 14-year-old black male Poodle was brought to the Animal Teaching Hospital, Udayana University by its owner with clinical signs of frequent licking of its left front paw. Upon examination, red bumps were observed on the left front leg, accompanied by small, round black spots scattered on the dorsal side of the body. Additionally, black nodules were present on the lower eyelids and hind limbs. Surgical intervention was undertaken to excise the tumor mass, with the animal under anesthesia induced by ketamine at 5 mg/kg BW intravenously. The reddish nodule was excised by performing an elliptical incision at the base of tumor. Postoperatively, the animal received an antibacterial injection comprising ceftriaxone and tazobactam at 25 mg/kg BW intramuscularly and antiseptic wound dressing for supportive care. Microscopic examination revealed neoplastic cells arranged into lobules of varying sizes and shapes within the tumor mass. These lobules consisted of differentiated sebocytes and basaloid cells. At the periphery of the neoplastic lobules, the basaloid cells displayed several layers and exhibited invasion with moderate anisocytosis. The mitotic index was no more than ten cells in one field of view. Based on these histopathological features, the tumor was confirmed to be a sebaceous adenoma. After a 10-month follow-up period, there were no signs of tumor recurrence observed.
Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models Marson, Fransiska Gratia Sonita; Sewoyo, Palagan Senopati; Astawa, I Nyoman Mantik; Adi, Anak Agung Ayu Mirah; Suardana, Ida Bagus Kade; Winaya, Ida Bagus Oka; Berata, I Ketut
Media Kedokteran Hewan Vol. 36 No. 1 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i1.2025.13-20

Abstract

c-Jun is often found to be overexpressed in various cancers, so this gene might be a target for cancer therapy. Newcastle disease virus (NDV) is recognized for its oncolytic properties and potential as a cancer virotherapy agent, with various mechanisms reported to trigger cancer cell death. This study aimed to assess the c-Jun expression in rat mammary cancer models. Rat mammary cancer models were categorized into two treatment groups: the control group (C) and the virotherapy group (V). Group C was administered with 0.5 cc of sterile PBS, while group V received 7 log 2 HAU per 0.5 cc of the Indonesian NDV field isolate Tabanan-1/ARP/2017 intratumorally. The treatment was carried out for four days in a row. Two weeks after treatment, all rats were humanely euthanized, and mammary cancer tissues were excised for further examination. Mammary cancer tissues were examined histopathologically and analyzed using immunohistochemistry to determine intranuclear c-Jun expression, quantified by the H-Score. The results demonstrated that NDV significantly reduced c-Jun expression. It can be inferred that NDV Tabanan-1/ARP/2017 holds potential as a mammary cancer therapy agent by reducing c-Jun expression. This finding is considered novel, as there have been no previous reports of decreased c-Jun expression following virotherapy with NDV.
The Role of p53 as the Guardian of the Genome and the Consequences of its Mutation in Cancer Development: A Review Purwitasari, Made Santi; Sewoyo, Palagan Senopati; Astawa, I Nyoman Mantik
Jurnal Medika Veterinaria Vol 19, No 2 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i2.48988

Abstract

The TP53 gene encodes the p53 protein, often referred to as the guardian of the genome due to its critical role in maintaining genomic stability and preventing tumorigenesis. Under normal conditions, p53 expression is tightly regulated by MDM2 and MDMX, which promote its degradation through ubiquitination. In response to endogenous or exogenous stress, this ubiquitination process is inhibited, leading to the stabilization of p53. Once stabilized, p53 forms a tetrameric complex in the nucleus and binds to DNA. It then activates the transcription of genes involved in cell cycle arrest, DNA repair, apoptosis, and senescence, aiming either to repair DNA damage or eliminate cells when the damage is irreparable. Nearly half of all cancer cases involve mutations in the TP53 gene. These mutations may include missense, nonsense, inframe, and splice-site mutations. Mutations in TP53 result in the production of mutant p53 (mutp53) proteins. These mutations can lead to a loss of tumor suppressor function or confer gain-of-function properties that promote tumor progression. Given its central role in cancer development, TP53 is considered a promising therapeutic target. Potential strategies include reactivating suppressed p53, restoring the function of mutp53, or inducing its degradation.
Case Report: Anatomic Pathology of Bali Cattle Liver Infested with Fasciola gigantica Sewoyo, Palagan Senopati; Purwanti, Ni Luh Lasmi; Evan, Samuel
Media Kedokteran Hewan Vol. 35 No. 1 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i1.2024.28-35

Abstract

Infectious diseases pose a significant threat to livestock health and productivity, particularly in developing countries. Fasciolosis, caused by infestation of Fasciola sp., is one such disease of concern. This case report focuses on the anatomic pathologic changes observed in the liver of Bali cattle infested with Fasciola gigantica. The cattle involved in this study were Bali cattle slaughtered at the Mambal Slaughterhouse in Badung, Bali, Indonesia between December 2021. During the specified period, 2 out of the 16 slaughtered cattles tested positive for Fasciola gigantica, representing an infection rate of 12.5%. The primary objective of composing this article is to disseminate information pertaining to the anatomical and pathological alterations observed in Bali cattle as a consequence of fasciolosis. Regarding the observation, the liver exhibited hepatomegaly, characterized by enlarged size and blunt edges, and adult flukes were found in the bile ducts. The surface of the liver parenchyma displayed proliferation of connective tissue, and there was evidence of enlarged portal lymph nodes. Based on these findings, it can be concluded that the Bali cattle in this case report were afflicted with fasciolosis.
Co-Authors Anak Agung Ayu Mirah Adi Evan, Samuel I Gusti Agung Gede Putra Pemayun I Ketut Berata I Made Merdana I NYOMAN MANTIK ASTAWA I Wayan Wirata Ida Bagus Kade Suardana Ida Bagus Oka Winaya Luh Made Sudimartini Marson, Fransiska Gratia Sonita Purwanti, Ni Luh Lasmi Purwitasari, Made Santi Putu Henrywaesa Sudipa