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All Journal Indonesia Medicus Veterinus Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters Journal of Veterinary and Animal Sciences Advances in Tropical Biodiversity and Environmental Sciences
Putu Henrywaesa Sudipa
Laboratorium Mikrobiologi Veteriner Fakultas Kedokteran Hewan Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Identifikasi dan Prevalensi Jamur Curvularia pada Anjing dan Kucing di Kabupaten Badung, Bali Tahun 2020 Sudipa, Putu Henrywaesa; Gelgel, Ketut Tono Pasek; Jayanti, Putu Devi
Indonesia Medicus Veterinus Vol 10 (3) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.3.432

Abstract

Curvularia adalah genus Pleosporalean monophyletic dengan banyak jenis spesies, termasuk jenis fitopatogenik, jamur patogen pada hewan dan manusia. Curvularia juga menyebabkan phaeohyphomycosis yang mana ditemukan pada invertebrata, vertebrata berdarah dingin, burung dan spesies mamalia termasuk ruminansia, kuda, anjing, kucing dan manusia. Penelitian ini bertujuan untuk mengidentifikasi dan mengetahui prevalensi Curvularia pada anjing dan kucing. Sampel diambil dengan cara menggunakan teknik sitologi kulit dengan menggunakan plester selotip (tape) yang ditempelkan pada bagian tubuh yang mengalami infeksi jamur kemudian ditempelkan pada gelas objek yang sudah diberikan beberapa tetes pewarna methylene blue. Sampel menggunakan 34 sampel (26 ekor anjing dan 8 ekor kucing) dan sampel diperiksa dibawah mikroskop cahaya dengan pembesaran 100 kali. Pemeriksaan mikroskopis sampel melihat karakteristik jamur, terutama pigmentasi, septasi, morfologi, ukuran hifa, konidiofor dan konidia. Dari data jenis jamur yang teridentifikasi kemudian data ditabulasi dan dijelaskan secara deskriptif. Hasil yang didapatkan adalah prevalensi Curvularia pada anjing adalah 19%, pada kucing 50% dan total infeksi Curvularia dari seluruh sampel berjumlah 26%. Culvularia yang teridentifikasi adalah tipe konidia tipikal yang bentuk konidiumnya obovoid, melengkung, agak membengkak pada satu sel, gelap, dan dindingnya tebal dan tipe konidia atipikal yang bentuknya lurus dan menyempit ke arah ujung, konidiofor tidak bercabang, dan berbentuk zigzag serta jumlah septa berjumlah 3-4 atau eusepta.
Madu Trigona Mampu Menghambat Pertumbuhan Jamur Curvularia sp. yang Diisolasi dari Anjing Patabang, Denselina Lilis; Suartha, I Nyoman; Sudipa, Putu Henrywaesa
Indonesia Medicus Veterinus Vol 11 (1) 2022
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2022.11.1.117

Abstract

Curvularia sp. merupakan jamur dermatiaceae atau jamur dengan pigmen hitam yang menyebabkan berbagai macam penyakit pada hewan. Peningkatan invasi dan proliferasi jamur Curvularia sp. diakibatkan oleh penggunaan bahan kimia secara berlebihan. Oleh karena itu, dibutuhkan alternatif lain dalam mengobati penyakit jamur yaitu dengan obat herbal. Madu trigona mengandung senyawa flavonoid dan polyphenol karena lebah trigona dapat mengumpulkan nektar dari bagian bunga. Tujuan dari penelitian ini adalah untuk mengetahui tentang efektivitas madu trigona dalam menghambat pertumbuhan jamur Curvularia sp. Penelitian ini bersifat eksperimental menggunakan rancangan acak lengkap terhadap enam perlakuan yaitu madu trigona konsentrasi 20%, 25%, 30% dan 100%, kontrol positif dan kontrol negatif terhadap biakan jamur Curvularia sp. pada media Sabouraud Dextrose Agar. Madu trigona kemudian di uji sensitivitasnya dengan teknik lubang sumuran pada media Sabouraud Dextrose Agar dan dilihat hingga terbentuknya zona hambat. Terbentuknya hambatan di sekitar lubang sumuran yang tidak ditumbuhi jamur menunjukkan hasil positif dan zona hambat dapat diukur. Data kemudian dianalisis dengan menggunakan sidik ragam. Hasil dari penelitian menunjukkan bahwa madu trigona konsentrasi 20%, 25%, 30% dan kontrol negatif tidak menghasilkan zona hambat (0 mm). Madu trigona konsentrasi 100% menghasilkan zona hambat sebesar 2,14 mm, sedangkan kontrol positif menghasilkan zona hambat sebesar 5,15 mm. Madu trigona yang dapat menghambat pertumbuhan jamur Curvularia sp. yaitu madu dengan konsentrasi 100%.
Bakteri Escherichia coli Teridentifikasi pada Rektum Lumba-Lumba Hidung Botol Indo-Pasifik di Umah Lumba Rehabilitation Center, Taman Nasional Bali Barat Kristiawan, Vicky; Mahatmi, Hapsari; Sudipa, Putu Henrywaesa; Rahmadani, Deny
Indonesia Medicus Veterinus Vol 11 (2) 2022
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2022.11.2.234

Abstract

Saluran cerna merupakan salah satu tempat pertumbuhan berbagai genus serta spesies bakteri termasuk bakteri family Enterobacteriaceae. Salah satu dari anggotanya adalah Eschericia coli yang merupakan flora normal yang sering ditemukan pada saluran cerna. Saat ini masih sangat langka penelitian tentang keberadaan E. coli pada saluran cerna lumba-lumba hidung botol Indo-Pasifik (Tursiops aduncus). Tujuan penelitian ini adalah untuk mendapatkan data awal mengenai bakteri Enterobateriaceae pada lumba-lumba tersebut yang berada di lingkungan rehabilitasi. Sampel lumba-lumba yang digunakann merupakan lumba-lumba hidung botol Indo-Pasifik (T. aduncus) yang berada di jaring apung laut atau seapen Umah Lumba Rehabititation Center, sebanyak tiga ekor. Pengambilan sampel dilakukan dengan menggunakan rubber catheter sterile berukuran 2,7 mm yang dimasukkan ke dalam rektum lumba-lumba. Jumlah total sampel yang di isolasi sebanyak 12 sampel dari tiga ekor lumba-lumba. Isolasi sampel dilakukan dengan menggunakan media Sheep Blood Agar, media Mac Conkey Agar dan Eosine Methylen Blue Agar. Selanjutnya di uji dengan pewarnaan Gram, uji katalase, uji oksidase, Triple Sugar Iron Agar, Sulfide Indole Motility, Methyl Red Voges Proskauer, Simmon Citrate Agar, dan uji urease. Hasil dari penelitian ini dapat disimpulkan pada tiga ekor lumba-lumba hidung botol Indo-Pasifik (T. aduncus) yang berada di seapen Umah Lumba Rehabilitation Center, ditemukan jenis bakteri famili Enterobacteriaceae spesies E. Coli.
Characteristics of Rumen Fluid, pH and Number of Microbia I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel; Putu Henrywaesa Sudipa
Journal of Veterinary and Animal Sciences Vol 4 No 1 (2021)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2021.v04.i01.p02

Abstract

Abstract. This research purpose is to determine the characteristics of pH rumen fluid and the amount of microbes in the rumen of Bali cattle. The research sample is healthy bali cattle rumen fluid which is slaughtered at Pesanggaran slaughterhouse Denpasar with a total of 30 samples. The research method uses conventional methods and refers to the method according to [6]. The total population of bacterial and fungal colonies was calculated by the pour plate method. Bacterial colonies were cultured on nutrient agar plates and fungi colonies were cultured on sabouraud dextrose agar plates. The Population of Enterobacteriaceae bacterial colonies was calculated by scatter method on Mac Conkey agar plate and Escherichia coli colony on Eosin Methylen Blue agar plate. Rumen liquid pH measurement is directly done with pH indicator strips. The study was conducted observationally and the data obtained were analyzed descriptively. The results showed that the total number of bacteria in the rumen fluid of Bali cattle with an average of 64x104 cfu/g, the number of fungal colonies with an average of 161x105 cfu/ g, the number of Coliform colonies was 23x104 cfu/g, the number of non-Coliform 28x103 cfu/g, E.coli 18x103 cfu/g and the mean The pH of Balinese cattle rumen fluid 6.9. Keywords : Balinese Cattle Rumen Fluid, Coliform, E. Coli , Fungi, non-Coliform, Total Bacteria , pH
Sebaceous Adenoma in a Geriatric Poodle Dog: A Case Report Winaya, Ida Bagus Oka; Adi, Anak Agung Ayu Mirah; Sudimartini, Luh Made; Merdana, I Made; Sudipa, Putu Henrywaesa; Pemayun, I Gusti Agung Gde Putra; Sewoyo, Palagan Senopati
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.413-419

Abstract

A 14-year-old black male Poodle was brought to the Animal Teaching Hospital, Udayana University by its owner with clinical signs of frequent licking of its left front paw. Upon examination, red bumps were observed on the left front leg, accompanied by small, round black spots scattered on the dorsal side of the body. Additionally, black nodules were present on the lower eyelids and hind limbs. Surgical intervention was undertaken to excise the tumor mass, with the animal under anesthesia induced by ketamine at 5 mg/kg BW intravenously. The reddish nodule was excised by performing an elliptical incision at the base of tumor. Postoperatively, the animal received an antibacterial injection comprising ceftriaxone and tazobactam at 25 mg/kg BW intramuscularly and antiseptic wound dressing for supportive care. Microscopic examination revealed neoplastic cells arranged into lobules of varying sizes and shapes within the tumor mass. These lobules consisted of differentiated sebocytes and basaloid cells. At the periphery of the neoplastic lobules, the basaloid cells displayed several layers and exhibited invasion with moderate anisocytosis. The mitotic index was no more than ten cells in one field of view. Based on these histopathological features, the tumor was confirmed to be a sebaceous adenoma. After a 10-month follow-up period, there were no signs of tumor recurrence observed.
Identification and Prevalence of Aspergillus sp Isolated from Bali Dog’s Skin Sudipa, Putu Henrywaesa; Puja, I Ketut; Dharmayuda, Anak Agung Gde Oka; Gunawan, I Wayan Nico Fajar; Sudimartini, Luh Made; Jayanti, Putu Devi; Sukernayasa, I Wayan; Mufa, Romy Muhammad Dary
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 1, JANUARY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i1.21594

Abstract

Aspergillus sp are fungi that commonly caused a systemic respiratory infection in Human and Dog. In Bali, most of the dog are free-roaming dogs, and has poor skin health condition. Some of them are suspected with fungal infection. Aspergillus sp has high risk to infect the dog, because they are available everywhere in environment. Therefore, this research is conducted for adding primary data of Aspergillus sp isolated from dog’s skin and their prevalence. The samples are 15 free-roaming Bali dogs with suspected fungal infection. Identification was carried out by using microscopic and macroscopic observation methods, skin swab was taken from the dog’s skin using the Mackenzie method, and the sterile toothbrush swab gently from the dog’s skin to the Sabouraud Dextrose Agar (SDA) media. The fungi were cultured on SDA media for macroscopic identification for approximately 3-4 days, mostly to observe how the fungal colony grew and its color. Microscopic identification are using adhesive tape, the tape was gently pressed into the fungal colony, the tape was stuck into the surface of object-glass with 3-4 drops of Methylene Blue staining, and the object glass was examined by using a light microscope, the findings of Aspergillus sp fungi are recorded and the data are descriptively presented. The result shows that most Aspergillus species that were found is Aspergillus fumigatus and Aspergillus niger according to their colonies growth and microscopic appearance, and the prevalence from 15 samples were 53.33%. Keywords: Aspergillus sp, fungi, Bali Dogs, Identification, Prevalence
Penyakit tetelo pada ayam lokal – Laporan kasus Hartaputera, I Nyoman Surya Tri; Kencana, Gusti Ayu Yuniati; Adi, Anak Agung Ayu Mira; Sudipa, Putu Henrywaesa; Sulabda, I Nyoman
ARSHI Veterinary Letters Vol. 8 No. 1 (2024): ARSHI Veterinary Letters - February 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.1.9-10

Abstract

Peternakan ayam lokal tradisional, dibandingkan dengan peternakan unggas komersial, menghadapi banyak tantangan terkait penyakit, dengan salah satu ancaman utama adalah virus penyakit tetelo. Laporan kasus ini menggambarkan ayam lokal berumur 8 minggu yang terinfeksi virus Newcastle disease terdeteksi melalui pemeriksaan aspek epidemiologi, patologi, dan virologi. Angka kesakitan di peternakan ini adalah 65%, dengan angka kematian 20% dan angka kematian 30,7%. Hasil pemeriksaan anatomi dan histopatologi menunjukkan adanya perubahan patologis pada berbagai organ. Uji laboratorium menggunakan uji Haemmaglutination-Inhibition dan Haemmaglutination memastikan adanya virus penyakit tetelo. Berdasarkan gejala klinis, temuan patologi anatomi, analisis histopatologi, isolasi virus, dan identifikasi, disimpulkan bahwa ayam yang terkena penyakit tersebut terinfeksi penyakit Newcastle velogenik viscerotropik.
Isolation and Antibiotic Resistance Profile of Klebsiella spp. from the Gastrointestinal Tract of Broiler Chickens in Poultry Farms in Bali Yeocelin Meida Utami; Putu Henrywaesa Sudipa; I Gusti Ketut Suarjana; I Nengah Kerta Besung; Hapsari Mahatmi; I Gede Putu Anggita Raka Saputra; Arine Rayka Khaniya
Advances in Tropical Biodiversity and Environmental Sciences Vol. 9 No. 2 (2025): June 2025
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2025.v09.i02.p02

Abstract

The poultry sector in Bali has experienced substantial growth, accompanied by a notable increase in broiler chicken populations. Information on Klebsiella spp. outbreaks in Bali has not yet been studied, despite the many potential infectious diseases that may affect broiler chickens on a large scale. Klebsiella spp. is an opportunistic pathogen that can induce severe respiratory disease in poultry, carrying a potential risk of zoonotic transmission. Furthermore, the increasing incidence of antibiotic resistance among Klebsiella strains poses a critical challenge to effective therapeutic interventions. This study was conducted to isolate and identify Klebsiella spp. from the gastrointestinal tract of broiler chickens in commercial farms across Bali, and to assess the antimicrobial resistance profiles of the isolates against multiple antibiotic agents. A cross-sectional study with a total of 25 cloacal swabs was conducted, and the swabs were subjected to microbiological analysis. Isolation was performed using selective MacConkey Agar (MCA), followed by Gram staining, biochemical identification, and antibiotic susceptibility testing through the Kirby-Bauer disk diffusion method. Two out of 25 (8%) samples were positively identified as Klebsiella spp. All of these positive isolates showed complete resistance to ampicillin, sulfamethoxazole, erythromycin, ciprofloxacin, and tetracycline. Additionally, the positive isolates also exhibited intermediate resistance against kanamycin, while 50% of the isolates demonstrated susceptibility to chloramphenicol. These findings confirm the presence of multidrug-resistant (MDR) strains among the isolates examined. The outcomes of this research emphasize the urgent need for a rigorous study to identify potential outbreaks caused by Klebsiella spp., throughout commercial farms in Bali and stringent monitoring and regulation of antibiotic usage within the poultry industry to mitigate the proliferation of antimicrobial resistance, thereby safeguarding animal health and preventing potential risks to public health.
Co-Authors Adi, Anak Agung Ayu Mira Anak Agung Ayu Mirah Adi Anak Agung Gde Oka Dharmayuda Anak Agung Gde Oka Dharmayudha Arine Rayka Khaniya Deny Rahmadani, Deny Gunawan, I Wayan Nico Fajar Gusti Ayu Yuniati Kencana Hapsari Mahatmi Hapsari Mahatmi Hartaputera, I Nyoman Surya Tri I Gede Putu Anggita Raka Saputra I Gusti Agung Gede Putra Pemayun I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Ketut Puja I Made Merdana I Made Sukada I Nengah Kerta Besung I Nyoman Suartha I Nyoman Sulabda I Wayan Wirata Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Kadek Karang Agustina Ketut Tono Pasek Gelgel Kristiawan, Vicky Luh Made Sudimartini Mufa, Romy Muhammad Dary Muh. Muazdzamzam Lil Abrori Ni Ketut Suwiti Ni Luh Eka Setiasih Patabang, Denselina Lilis Putu Devi Jayanti Sewoyo, Palagan Senopati Sukernayasa, I Wayan Syafiana Fairizca Yeocelin Meida Utami