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IDENTIFICATION OF SCCMEC TYPE IN ISOLATE OF METHICILLIN RESISTANT Staphylococcus aureus (MRSA) IN JAMBI BY USING POLYMERASE CHAIN REACTION (PCR) Humaryanto, Humaryanto; Hanina, Hanina; Lipinwati, Lipinwati; Simanjuntak, Charles Apul
Indonesian Journal of Tropical and Infectious Disease Vol. 8 No. 2 (2020)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v8i2.12691

Abstract

Staphylococcal cassette chromosome mec (SCCmec) is one of the mobile genetic elements of Methicillin-Resistant Staphylococcus aureus (MRSA) that carries many resistance genes and allows SCCmec to move from one bacterium to another. Twelve types of SCCmec have been identified throughout the world. Identification of SCCmec type is needed to determine the pattern of MRSA resistance in a particular region. This study aimed to identify the type of SCCmec MRSA from clinical samples. Specifically, this study was conducted at the Biomolecular Laboratory of the Faculty of Medicine and Health Sciences of Jambi University in June 2018-February 2019. Culture was carried out on 100 clinical specimens of festering wound swabs from inpatients at hopitals in Jambi City. A total of 32 samples of Staphytect plus test positive were tested using Cefoxitin disc diffusion method and MecA Polymerase Chain Reaction (PCR). There were 14 samples identified as MRSA isolates, namely twelve samples (85.72%) of SCCmec type III, one sample (7.14%) of SCCmec type II, and one sample (7.14%) of SCCmec type IVb. The results were different from previous studies where all MRSA isolates (100%) in Indonesia were SCCmec type III, although most SCCmec types were still dominated by SCCmec type III. This study concludes that there has been a shift in the content of SCCmec in MRSA isolate originating from hospitals in Jambi city.
Effect of essential oil on hypertrophic scars Rahman, Ave Olivia; Humaryanto, Humaryanto; Purwakanthi, Anati; Simanjuntak, Charles Apul
MEDISAINS Vol 19, No 1 (2021)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v19i1.10192

Abstract

Background: Hypertrophic scars are abnormal scars resulted from a disrupted wound healing process. Hypertrophic scars can affect the body aesthetic of the sufferers, but, on the other hand, conventional therapy has not been optimally effective. The application of essential oil combinations as an alternative therapy for hypertrophic scars has not been scientifically proven.Purposes: This study was to determine the effectiveness of the essential oil as a hypertrophic scar therapy.Methods: This study used a pre and post-test design. As many as 24 subjects aged 17-25 years with hypertrophic scars were given essential oil combinations every morning and evening for six months. Assessment of hypertrophic scars was done using the POSAS  (Patient and Observer Scar Assessment Scale).Results: The results showed that the POSAS score decreased after the essential oil combination administration for six months, 14.40±6.08 vs. 22.67±8.31 (p<0.001).Conclusion: the combination of essential oil oils used in this study reduces hypertrophic scars based on the POSAS assessment.
DETEKSI DINI DAN EDUKASI ORANG TUA TENTANG GANGGUAN TUMBUH KEMBANG BALITA Simanjuntak, Charles Apul; Fitri, Amelia Dwi; S, Nys. Natasha Ayu; Puspasari, Anggelia
Jurnal Karya Abdi Masyarakat Vol. 1 No. 1 (2017): Jurnal Karya Abdi Masyarakat
Publisher : LPPM Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.959 KB) | DOI: 10.22437/jkam.v1i1.3723

Abstract

Orang tua merupakan pihak yang sangat berpengaruh dalam pemantauan maupun untuk pemenuhan kebutuhan tumbuh kembang anak terutama pada lima tahun kehidupan yang merupakan masa keemasan bagi tumbuh kembang anak. Data dari riset menunjukkan peningkatan prevalensi orangtua yang tidak melakukan pemantauan tumbuh kembang anak berkala, padahal pemantauan tumbuh kembang balita selaras dengan deteksi dini gangguan tumbuh kembang balita sehingga intervensi dan simulasi dapat memberikan hasil yang optimal. Oleh karena hal tersebut penulis mengusulkan pengabdian masyarakat ini. Pengabdian masyarakat diselenggarakan di klinik social MER-C didapatkan sampel sebanyak 66 anak dalam kurun waktu Mei hingga Juni 2016. Kegiatan pemantauan tumbuh kembang dilakukan sesuai kurva pertumbuhan WHO dengan pengukuran berat badan dan tinggi badan. Pemantauan perkembangan anak dilakukan dengan instrument KPSP. Edukasi kepada orangtua dilakukan dengan penyuluhan tentang tumbuh kembang anak dan asupan nutrisi yang bergizi dan sesuai. Hasil deteksi dini penilaian pertumbuhan dengan kurva WHO didapatkan sebanyak 53% anak yang diperiksa memiliki perawakan normal dan gizi normal. Sebanyak 31, 7 % anak yang diperiksa memiliki masalah gizi lebih dengan rincian 15,1 % terdeteksi resiko gizi lebih, 4,5 % overweight dan 12, 1 % obesitas. Pemeriksaan perkembangan anak dengan instrument KPSP didapatkan sebanyak 97% anak perkembangannya sesuai dengan usia, hanya 3% atau 2 orang dari 66 anak yang diperiksa nilai KPSP meragukan. Edukasi yang diberikan kepada orang tua berupa pentingnya orangtua memeriksa pertumbuhan dan perkembangan balita, waktu dan kekerapan orangtua melakukan pemeriksaan dan bagaimana memberikan asupan gizi daan stimulasi yang benar dan seimbang sesuai usia anak sehingga tercapai tumbuh kembang anak yang optimal.