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All Journal Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) MEDIC MEdical Dedication Jurnal Karya Abdi Masyarakat Electronic Journal Scientific of Environmental Health And Diseases Riset Informasi Kesehatan Journal of Medical Studies Neurona

Lipinwati ., Lipinwati

FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Author-ID : 560407

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Other

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Diagnosis Molekuler Human Papilloma Virus (HPV) Penyebab Kanker Serviks Lipinwati .
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 2 No. 1 (2014): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

ABSTRACT Cervical cancer is still the one of major public health problem in the world whether an effective screening programs using the Papanicolaou test to detect precancerous lesions has reduced the number of cases of cervical cancer in the developed countries but not in developing countries. The sensitivity and specificity of the Pap smear depends on observer’s skills to recognize and classify variations of abnormal cells. Human Papilloma Virus (HPV) caused cervical cancer has been proved, In some study have found HPV DNA in 99.7 % of all cervical carcinomas. HPV has been found to be more than 100 genotypes , which are divided into high-risk HPV and low-risk HPV The development of early diagnosis for the detection of HPV infection still has been a problem, where the cytology and colposcopy to identify lesions at an advanced stage, therefore the molecular engineering approach seems to be more successful in early diagnosing cervical cancer. This technique of molecular HPV DNA sequences are Southern Blot Hybridization, Hybrid Capture II Assay, Polymerase Chain Reaction, and PCR - Reverse Line Blot. The Accuracy of molecular diagnostic techniques is required to provide further information for patient how the management and maintenance of cervical cancer. Keywords: Cervical cancer, HPV, screening, molecular techniques

BACTERIOLOGICAL TEST OF WATER FROM DUG WELL THAT DOES NOT FULFILL REQUIREMENT IN THE WORKING AREA PUTRI AYU HEALTH CENTERS, SUB TELANAI PURA OF JAMBI, 2015 Lipinwati - -; Aromatika L; Armaidi Darmawan
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 4 No. 1 (2016): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Abstract Background: The incidence diarrhea in Jambi is still quite high, especially in infants with 10936 cases. The highest incidence was in Putri Ayu health centers. One of the associated factors with diarrhea is water supply does not fulfill requirement. In the working area of Putri Ayu health centers, most people use dug well as one of the sources of water supply, but there are some dug wells have unfulfill requirement physical conditions. Dug well water is vulnerable to contamination by bacteria. The purpose of this study was to know the MPN index of coliform bacteria, the microscopic images of coliform bacteria and the quality of dug well water. Methode : This study was a descriptive with laboratory experimental design. The samples were 25 unfulfill requirement dug well water that conducted using proportionate stratified random sampling method from April to July 2015. Result : From 25 samples, there are 21 water samples (84%) has a number of coliform that over the maximum levels are allowed by SK. Dirjen PPM and PLP No. 1/PO.03.04.PA.91 and SK JUKLAK PKA 2000/2001, that is 50 MPN/100 ml. From microscopic images, there are 19 samples (76%) had gram-negative bacteria with red and short rod-shaped and six other samples (24%) is a gram-positive bacteria with purple and cocci shaped. Conclusion: There are coliform bacteria contamination in dug well water in the working area of Putri Ayu health centers. This result might be influenced by physical condition of dug well. Keywords: Dug well water, coliform, MPN

Bacterial Profile and Antibiogram of Hospital-Acquired Pneumonia and Ventilator-Associated Pneumonia Patients in ICU of Raden Mattaher Hospital Jambi Sotianingsih Sotianingsih; Samsirun H; Lipinwati Lipinwati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 3 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i3.1596

Pneumonia is defined as an inflammation of the lungs caused by microorganisms (bacteria, viruses, fungi, parasites). This research aimed to determine the pneumonia-causing bacteria along with the sensitivity and the antibiotic resistance test. This research was a descriptive study with samples of ICU pneumonia patients at Raden Mattaher Regional Hospital during the study period. All samples were consecutively selected. Samples for blood culture were incubated in the BactAlert device, whereas the sensitivity test was then performed using Vitex instruments. Sputum was previously enriched with BHI media and then cultured on culture media, and sensitivity test with the Vitex instruments was carried out. Of the 354 ICU patients during the study period, 30 patients (11.8%) had pneumonia, but only 19 patients could undergo sputum culture. Five of 19 patients were infected with Gram-positive bacteria, and 14 patients were infected with Gram-negative bacteria. The most commonly found bacteria were Klebsiella pneumonia (36.84%), followed by Acinetobacter baumanii (21.05%) and Pseudomonas aeruginosa (10.53%). Gram-negative bacteria obtained from sputum culture in this study were resistant to almost all antibiotic groups, especially penicillin, cephalosporin, quinolone, and tetracycline groups. Gram-positive bacteria obtained from sputum culture in this study were resistant to the penicillin antibiotic. The most commonly found bacteria were Klebsiella pneumonia (36.84%), followed by Acinetobacter baumanii (21.05%) and Pseudomonas aeruginosa (10.53%). The bacteria cultured from the sputum showed multidrug resistance mainly to the penicillin and cephalosporin antibiotic. This research data can be used to consider the treatment of pneumonia patients to decide more appropriate therapy.

PENINGKATAN PENGETAHUAN KESEHATAN REPRODUKSI DAN PENYAKIT MENULAR SEKSUAL PADA SISWA/I SMA NEGERI 5 KOTA JAMBI Lipinwati Lipinwati; Maria Estela Karolina; Resty Hardianty Lestari
Medical Dedication (medic) : Jurnal Pengabdian kepada Masyarakat FKIK UNJA Vol. 1 No. 2 (2018): MEDIC: Medical Dedication
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/medicaldedication.v1i2.4952

ABSTRAK Pendahuluan : seksualitas merupakan sesuatu yang berkaitan dengan reproduksi serta semua aspek yang berkaitan dari sisi fisik, biologis, psikis serta sosial yang berhubungan dengan proses reproduksi pada manusia. Masa remaja adalah masa dimana seorang individu mengalami peralihan dari masa kanak- kanak ke masa dewasa disertai dengan perubahan baik fisik, psikis maupun perilaku secara radikal, sehingga remaja mulai bertanya-tanya mengenai berbagai fenomena yang terjadi di lingkungan sekitarnya sebagai dasar bagi pembentukan nilai diri mereka. Metode : kegiatan ini dilakukan dengan metode penyuluhan, yaitu dengan memberikan edukasi secara langsung pada Siswa dan Siswi SMA Negeri 5 Kota Jambi mengenai materi Kesehatan Reproduksi Remaja dan Infeksi Menular Seksual Hasil : Berdasarkan analisis data tersebut dapat disimpulkan bahwa terdapat peningkatan pengetahuan setelah diberikan penyuluhan tentang Kesehatan Reproduksi dan Penyakit Menular Seksual pada Siswa/I SMA Negeri 5 Kota Jambi. Kesimpulan : Penyuluhan kesehatan adalah salah satu bentuk promosi kesehatan yang sederhana dan dapat mencakup sasaran yang luas. Dari hasil penyuluhan tentang Pengetahuan Kesehatan Reproduksi Remaja dan Penyakit Infeksi Menular Seksual yang telah dilakukan, dapat diambil kesimpulan bahwa terdapat perbedaan nilai rata-rata sebelum dan setelah dilakukan penyuluhan kesehatan tentang Kesehatan Reproduksi dan Penyakit Infeksi Menular Seksual. Keywords: Kesehatan Reproduksi, Penyakit Menular Seksual

SKRINING KELAINAN KULIT PADA PELAJAR PONDOK PESANTREN NURUL IMAN DI KOTA JAMBI Lipinwati Husman; Rhonaz Putra Agung; Maya Arini Hestiningtyas; Maria Estela Karolina; Mara Imam Taufik Siregar
Medical Dedication (medic) : Jurnal Pengabdian kepada Masyarakat FKIK UNJA Vol. 5 No. 2 (2022): MEDIC. Medical dedication
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Pondok pesantren sering dihadapkan pada masalah Kesehatan dengan berbagai macam jenis penyakit seperti penyakit kulit dan TB. Kelainan kulit berpotensi menular jika salah satu pelajar tidak menjalankan Perilaku Hidup Bersih dan Sehat (PHBS). Pemeriksaan langsung secara mikroskopis merupakan pemeriksaan yang paling mudah, murah dan spesifik . Untuk itu tim pengabdian masyarakat Fakultas Kedokteran dan ilmu Kesehatan Universitas Jambi (FKIK UNJA) memberikan penyuluhan mengenai PHBS, melakukan pemeriksaan kerokan kulit, dan pengobatannya. Penyuluhan PHBS, Pemeriksaan langsung kerokan kulit dilakukan dengan mengerok bagian kelainan kulit menggunakan skalpel diatas objek gelas dan diberikan 1 tetes larutan KOH 10%, Pembacaan hasil menggunakan mikroskop, dan pengobatan yang diberikan yang sesuai. Pengabdian dilaksanakan 24 September 2022 di ponpes Nurul Iman. Penyuluhan diikuti oleh 285 orang pelajar MTs. Nurul Iman, Kota Jambi dan peserta yang melakukan pemeriksaan langsung kerokan kulit ada 23 orang. Pada pelajar dengan kelainan kulit terbanyak adalah laki-laki, umur 13 tahun. pemeriksaan langsung kerokan kulit banyak yang tidak ditemukan spora/hifa/tungau, dan diagnosis scabies terbanyak di antara jenis kelainan kulit lain. Adanya peningkatan pengetahuan para pelajar mengenai PHBS, telah dilakukan pemeriksaan langsung kerokan kulit, dan pemberian terapi sehingga diharapkan dapat mencegah penularan penyakit kulit. Keyword: Kelainan kulit, Pondok pesantren, Kerokan kulit

Gambaran Obat Tradisional yang Digunakan Penderita Malaria di Wilayah Puskesmas Simpang IV Sipin Kota Jambi 2014 Darmawan, Armaidi; ., Lipinwati
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 2 No. 2 (2014): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

ABSTRACT Background: Traditional medicine is the ingredients derived from plants, animals, minerals or mixtures of these materials. Malaria is an infectious disease caused by Plasmodium parasites that invade erythrocytes and characterized by the discovery of asexual blood forms. At Jambi province, the morbidity due to malaria is still quite high. There are nine districts and two municipalities which is a malaria endemic area. The proportion use of traditional medicine for malaria is still unknown include Jambi province. The number of people and the efficacy of using a traditional medicine remains unknown. This research designed in order to describe the use of traditional medicine in patients with malaria. Methods: The type of this research is descriptive quantitative research. The study was conducted at the Puskesmas Simpang IV Sipin Jambi. The entire study of population malaria patients that visiting Puskesmas Simpang IV Sipin and home visit patients who had suffered from malaria in the month of November 2013-February 2014. The number of samples in this study is 70 respondents. Data were analyzed by univariate analysis. Result: From the results, users of traditional medicine as much as 34.5%, traditional types of drugs most widely used is the use of sambiloto (27.1%), the most reason way the patients take traditional medicine because the side effects are relatively small (35.7%), the most way to mix the ingredients is by blend the ingredients together boiled it whit water, after that patients drunk the potions (80%), the most way to find the ingredients is looking around the house (80%), the most duration of using traditional medicine expressed by respondents is for 3 days (41.4%), and 95.7% expressed the feeling that the traditional treatment is efficacious. Conclusion: The results stated that the description of the use of traditional medicine in Puskesmas Simpang IV Sipin is 34.5%. Keywords: Traditional medicine, malaria

EFEKTIVITAS MENCUCI TANGAN DENGAN SABUN CUCI TANGAN CAIR BERBAHAN AKTIF TRICLOCARBAN PADA MAHASISWA FAKULTAS KEDOKTERAN UNIVERSITAS JAMBI ANGKATAN 2015 lipinwati, lipinwati; Siska, meliana; Permana, Oki
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 5 No. 1 (2017): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Abstract Background: Hand hygiene is essential to prevent various infectious diseases caused by bactery of hands which can contaminate hands in a short time during daily activities. Indonesian people who wash their hands with soap only 12%. One of the methods hand washing in removing dirt and germs on the hands is Seven-step hand washing. Many types of soaps are used by people to wash their hands and Triclocarban is one of the active ingredient of hand washing soap. The aim of this study was to determine the effectiveness of seven-step of hand-washing with liquid Triclocarban soap in hand hygiene.Method : This study was an analytic study by using one group pretest-posttest experiment. The study took place in biomedic laboratorium Faculty of Medicine and Health Sciences University Jambi from January to March 2016. The samples were 36 swab cottons stick from sidelines right fingers students before and after handwash then analyzed with statistic. Result : The average number of bacterial colonies before and after hand wash were 28,41 and 7,12 CFU/Cm2. The study was considered significant because the results of Wilcoxon test show p<0.15 was 0,000. The bactery found mostly was cocci gram-positive and the least was bacilli gram-negative. Conclusion : Seven-steps hand washing with liquid triclocarban soap is effective in maintaining the cleanliness of hands. Keywords : Hand wash with soap, Seven steps hand washing, Triclocarban Abstrak Latar Belakang : Kebersihan tangan penting untuk mencegah penyakit infeksi karena mikroba mengkontaminasi tangan dalam waktu yang singkat dalam aktivitas sehari-hari. Rata-rata hanya 12% masyarakat Indonesia yang mencuci tangan pakai sabun. Metode cuci tangan tujuh langkah adalah metode cuci tangan paling lengkap dalam menghilangkan kotoran dan kuman yang ada di tangan. Banyak jenis sabun yang digunakan untuk mencuci tangan, salah satunya sabun cuci tangan berbahan aktif Triclocarban. Tujuan penelitian ini adalah untuk mengetahui efektivitas mencuci tangan tujuh langkah dengan sabun cuci tangan cair Triclocarban dalam menjaga kebersihan tangan. Metode : Jenis penelitian ini adalah penelitian analitik one group pretest-posttest eksperimen. Lokasi di laboratorium Biomedik FKIK Universias Jambi dari Januari sampai Maret 2016. Sampel penelitian ini adalah usapan kuman dengan kapas lidi di sela-sela jari tangan kanan mahasiswa sebelum dan sesudah cuci tangan sebanyak 36 orang yang kemudian dianalisis dengan menggunakan uji wilcoxon. Hasil : Jumlah koloni bakteri rata-rata sebelum dan sesudah cuci tangan adalah 28.41 dan 7.12 CFU/Cm2. Penelitian ini dianggap bermakna karena pada hasil analisis uji Wilcoxon didapatkan nilai p < 0,15 yakni 0,000. Bakteri yang paling banyak didapatkan adalah kokus gram positif dan paling sedikit batang gram negatif. Kesimpulan : Mencuci tangan tujuh langkah dengan sabun cuci tangan cair Triclocarban efektif dalam menjaga kebersihan tangan. Kata Kunci : CTPS, Cuci tangan tujuh langkah, Triclocarban

PENGETAHUAN PASIEN HIPERTENSI TERHADAP DIET RENDAH GARAM SEBELUM DAN SESUDAH DIBERIKAN KONSULTASI GIZI DI POLI GIZI RUMAH SAKIT RADEN MATTAHER TAHUN 2017 Lipinwati, Lipinwati
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 5 No. 2 (2017): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

ABSTRAK Latar Belakang : Tahun 2013 jumlah penduduk di indonesia yang menderita hipertensi sebanyak 65 juta jiwa dari 252 juta penduduk. Dalam penatalaksanaan non-farmakologi hipertensi, diet rendah garam sangat diperlukan dapat menurunkan sebanyak 2 -8 mgHg tekanan darah. Pengetahuan yang baik tentang diet rendah garam dapat mengontrol tekanan darah bagi pasien hipertensi dengan mengkomsumsi garam tidak lebih dari 2,4 g/hari. Pasien hipertensi sebaiknya diberikan konsultasi gizi untuk meningkatkan pengetahuan tentang diet rendah garam. Dari hasil data rekam medik RSUD raden Mattaher pada januari-maret 2016 jumlah kasus baru pasien hipertensi 60 pasien dengan jumlah kunjungan 586 pasien dan tidak semua pasien mendapatkan konsultasi gizi. Tujuan penelitian ini mengetahui perbedaan ni lai pengetahuan pasien hipertensi sebelum dan sesudah diberikan konsultasi gizi. Metode : Desain penelitian ini adalah Pre- ekperimen dengan one grup pretest posttest design. Teknik pengambilan sampel menggunakan purposive sampling. Sampel adalah 20 pasien hipertensi yang melakukan konsultasi gizi di RSUD Raden Mattaher. Sebelum dan sesudah konsultasi gizi pasien diminta menjawab kuesioner yang terdiri dari 12 pertanyaan. konsultasi diet rendah garam diberikan oleh ahli gizi sesuai dengan materi yang telah ditetapkan. Analisis data menggunakan uji t. Hasil : Perbedaan rata-rata skor sebelum diberikan konsultasi gizi adalah 67,90 dan rata -rata skor sesudah diberikan konsultasi gizi adalah 87,90 rata -rata skor pengetahuan sebelum dan sesudah diberikan konsultasi gizi adalah 20,00 dengan nilai signifikansi 0,000<0,05.Dari hasil analisis data dengan uji t didapatkan ada perbedaan pengetahuan sesudah diberikan konsultasi gizi Kesimpulan : Ada peningkatan pengetahuan setelah diberikan konsultasi gizi. Kata Kunci : Hipertensi, Diet Rendah Garam, Konsultasi Gizi ABSTRACT Background: In 2013 total population in Indonesia suffering from hypertension as much as 65 million people from 252 million population. In the non-pharmacological management of hypertension, a low-salt diet can reduce as much as 2-8 mgHg blood pressure. A good knowledge of low-salt diet can control blood pressure for hypertensive patients by consuming no more 2.4 g/ day. Hypertensive patients should be given nutritional assistance to improve knowledge about low-salt diet. From the results of medical record at Regional Public Hospital Raden Mattaher in january to March 2016 the number of new cases of hypertensive patients 60 patients with the number of visits 586 patients but not all patients get nutrition consultation. The purpose of this study was to know the difference in the value of knowledge of hypertensive patients before and after being given nutritional consultation. Methods: This Pre- experiment study used one group pretest posttest design. Sampling technique using non probability sampling that purposive sampling. With 20 hypertensive patients, who conducted nutritional counseling at Regional public hospital Raden Mattaher. Before and after the nutrition consultation the patient was asked to answer a questionnaire used consist 12 questions. Low-salt diet consultancy is given by nutritionists in accordance with the material that has been set. Data analysis with t test. Result: The mean difference score before given a nutritional consultation was 67.90 and the mean score after given a nutritional consultation was 87.90. The mean difference between the pre and post nutritional score was 20.00 with a significance value of 0.000 <0.05. From the results of data analysis with t test there is a difference of knowledge after given a nutritional consultation. Conclusion: There is improvement knowledge after being given a nutrisional consultation. Keywords: Hypertension, Low Salt Diet, Nutrition Consultation

Uji Kualitas Air Minum Isi Ulang di Kota Jambi JMJ, Jurnal; Lipinwati, Lipinwati; Darmawan, Armaidi; Kusdiyah, Erni; Karolina, Maria Estela
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 4 No. 2 (2016): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Abstract Background: Clean water for drinking is rare where the source has been contaminated with various kinds of waste, such as disposal of organic waste, household and toxic waste from the industry, so that ground water is also not safe to become a drinking water because it has been contaminated from the septic tank or surface water. Bottled drinking water is a choice for clean drinking water but the price of bottled drinking water is high enough and makes consumers look for the cheaper and the new alternatives such as refill drinking water. Depo drinking water refills continue to increase in line with the public needs of drinking water quality and safe for consumption, though cheaper, not all depo of drinking water refills are guaranteed product security. This study is to know quality test of the drinking water refill in Jambi City Method: This descriptive study with experimental research laboratory design was conducted in Jambi city with 62 samples from 11 sub-districts in Jambi city and conducted in Biomedical Laboratory of FKIK UNJA. Water samples were conducted by 3 stages of examination, ie the prediction test, the strengthening test and the complementary test with 5 tubes. This research was conducted in May - October 2016. Data study were shown in tables. Result: The results showed that the drinking water refill category either amounted to 20 depots (32.26%) and drinking water refill the bad category amounted to 42 depots (67.74%). of 42 samples of refill drinking water with positive probability test results, there were 16 samples (38,10%) containing faecal coliform, and 27 samples (64,29%) containing non-faecal coliform. Conclusion: Drinking Water Refills in Jambi City are not all free from koliform bacteria. Keywords: Drinking Water Refill, Bacteriological Test, Most Probable Number Abstrak Latar Belakang: Air bersih yang layak minum kian langka untuk dijumpai dimana sungai-sungai yang menjadi sumbernya sudah tercemar berbagai macam limbah, seperti buangan sampah organik, rumah tangga hingga limbah beracun dari industri. Air tanah juga sudah tidak aman dijadikan bahan air minum karena telah terkontaminasi rembesan dari tangki septik maupun air permukaan. Air minum dalam kemasan (AMDK) kini menjadi pilihan pemakaian air bersih namun harga AMDK cukup tinggi dan membuat konsumen mencari alternatif baru yang murah seperti air minum isi ulang. Depo air minum isi ulang terus meningkat sejalan dengan keperluan masyarakat terhadap air minum yang bermutu dan aman untuk dikonsumsi, meski lebih murah, tidak semua depo air minum isi ulang ini terjamin keamanan produknya. Penelitian ini bertujuan untuk mengetahui uji Kualitas bakteriologis Air Minum isi Ulang di Kota Jambi. Metode: Penelitian deskriptif dengan rancangan penelitian eksperimen laboratorium ini dilakukan pada 62 sampel dari 11 kecamatan kota Jambi. Pengujian bateriologis dilakukan menggunakan Most Probable Number yang terdiri dari 3 tahap (uji penduga, uji penguat dan uji pelengkap) dengan seri 5 tabung pada sampel air minum yang dilaksanakan di Laboratorium Biomedik FKIK UNJA. Penelitian ini dilakukan pada bulan Mei – Oktober 2016. Data disajikan dalam bentuk tabel. Hasil:. Hasil penelitian memperlihatkan bahwa air minum isi ulang kategori baik berjumlah 20 depot (32,26%) dan air minum isi ulang kategori buruk berjumlah 42 depot (67,74%). Dari 42 sampel air minum isi ulang dengan hasil uji penduga positif, terdapat 16 sampel (38,10%) yang mengandung koliform fekal, dan 27 sampel (64,29%) yang mengandung koliform non-fekal. Kesimpulan: Air Minum isi Ulang yang ada di Kota Jambi tidak semuanya bebas dari koliform fekal. Kata Kunci: Air Minum Isi Ulang, Uji Bakteriologis, Most Probable Number

PENGARUH PEMBERIAN EKSTRAK ETHANOL BIJI BUAH PINANG (ARECA CATECHU L.) TERHADAP PERTUMBUHAN STAPHYLOCOCUS AUREUS SECARA IN VITRO Baiti, MIftakhul; Elfrida, Solha; Lipinwati, Lipinwati
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol. 6 No. 1 (2018): JAMBI MEDICAL JOURNAL Jurnal Kedokteran Dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

ABSTRACT Background: Infectious disease is known as a global concern because of the irrational, excessive and long term use of antibiotics especially in Staphylococcus aureus infection. The unecessarily use of antibiotics can creat a resistance issue such as MSSA, MRSA, VISA and VRSA. So as a new alternative that is being developed in medicine, herbal plants are used. Areca nut (Areca catechu. L) is a herbal plant that is found in Indonesia, especially in Province of Jambi, betel nut is one of the best in the world. The aim of this study are to investigate phytochemical components and inhibition effect Staphylococcus aureus ATCC 25923 using dry ethanol extract of betel nuts at concentrations 20%, 30%, 40% and 50% in vitro. Method: Antibacterial sensitivity tests against Staphylococcus aureus ATCC 25923 were performed using dry ethanol extract of betel nuts (Areca catechu. L) with various concentrations on each group. Group I is treated nut with a concentration of 20%, Group II with a concentration of 30%, Group III with a concentration of 40%, Group IV with a concentration of 50%, the group V as a negative control (distilled) and group VI as a positive control that was given amoxicillin clavulanate 30μg. Then the inhibitory effects of these treatments are measeure and classified by the Davis and Stout classification in 1971. The data analysis began with Saphiro Wilk test and then with levent test statistic. Because distributed data is not normal, the analysis continued with Kruskal Wallis test and Post Hoc test. Results: Results of this study showed that the ethanol extract of betel nuts can inhibit the growth of S. aureus, which is a concentration of 20% with a diameter of 13,63mm, concentration of 30% with a diameter of 15mm, concentration of 40% with a diameter of 15,5mm, concentration of 50% with a diameter of 14,7mm compared to the positive control 30μg amoxicillin clavulanate in inhibiting the growth of S. aureus with a diameter of 34,25mm. Conclusion: The ethanol extract of betel nuts can inhibit the growth of Staphylococcus aureus in vitro. Areca seed extract with concentration of 40% is the optimum concentration to inhibit the growth of Staphylococcus aureus. Keywords: Staphylococcus aureus, Areca catechu. L, Sensitivity Test. ABSTRAK Latar Belakang : Penyakit infeksi merupakan masalah yang menjadi perhatian global, oleh karena penggunaan antibiotik yang irasional, berlebihan dan dalam jangka waktu yang lama, terurama infeksi yang disebabkan oleh Staphylococcus aureus. Hal ini dapat menimbulkan masalah baru resistensi antibiotik seperti MSSA, MRSA, VISA dan VRSA. Maka sebagai alternatif baru yang sedang dikembangkan dalam pengobatan yaitu dengan menggunakan tanaman herbal. Pinang (Areca catechu. L) merupakan tanaman herbal yang banyak ditemukan di Indonesia terutama Propinsi Jambi, pinang ini merupakan salah satu pinang terbaik di dunia. Tujuan dari penelitian ini adalah untuk mengetahui komponen fitokimia dan daya hambat ekstrak ethanol biji buah pinang terhadap pertumbuhan Staphylococcus aureus ATCC 25923 secara in vitro pada konsentrasi 20%, 30%, 40% dan 50%. Metode : Ekstrak ethanol biji buah pinang kering (Areca catechu. L) dilakukan uji sensitifitas antibakteri terhadap Staphylococcus aureus ATCC 25923 dengan berbagai konsentrasi pada masing – masing kelompok. Kelompok I yaitu perlakuan pinang dengan konsentrasi 20%, kelompok II dengan konsentrasi 30%, kelompok III dengan konsentrasi 40%, kelompok IV dengan konsentrasi 50%, kelompok V sebagai kontrol negatif (Aquades) dan kelompok VI sebagai kontrol positif dengan diberikan Amoksisilin Klavulanat 30µg. Kemudian diukur efek inhibisi dari perlakuan tersebut dan digolongkan berdasarkan penggolongan Davis and Stout 1971. Analisis data diawali dengan uji Saphiro-Wilk dan dilanjutkan dengan uji Levene, oleh karena data terdistribusi tidak normal maka dilakukan uji Kruskal Wallis dan uji Post Hoc. Hasil : Hasi penelitian menunjukkan bahwa ekstrak ethanol biji buah pinang dapat menghambat pertumbuhan S. aureus, yaitu konsentrasi 20% dengan diameter 13,63mm, konsentrasi 30% dengan diameter 15mm, konsentrasi 40% dengan diameter 15,5mm, konsentrasi 50% dengan diameter 14,7mm. Terdapat pengaruh Areca catechu. L dalam menghambat pertumbuhan Staphylococcus aureus ATCC 25923. Kesimpulan : Ekstrak ethanol biji buah pinang dapat menghambat pertumbuhan Staphylococcus aureus secara in vitro. Konsentrasi ekstrak biji buah pinang 40% merupakan konsentrasi optimum dalam menghambat pertumbuhan Staphylococcus aureus. Kata Kunci : Staphylococcus aureus, Areca catechu. L, Uji Sensitifitas.

Co-Authors Ahmad Syauqy Ahmad Syauqy Aidil Rahmat Ilham Amatullah, Afifah Armaidi Darmawan Aromatika L Attiya Istarini ATTIYA ISTARINI Attiya Istarini Baiti, MIftakhul Denok Tri Hardiningsih dewi, hasna Efendy, Makruf Ekaputri, Tia Wida Elfrida, Solha Erny Kusdiyah Esa Indah Ayu, Esa Indah Fadillah, Tanty Fairuz Quzwain Fairuz, Fairuz Fitriyanti Fitriyanti Fitriyanti Hafizah Hafizah Hanina Hanina, Hanina Hasna Dewi Hestiningtyas, Maya Humaryanto, Humaryanto Iskandar, Mirna Marhami Ivo Amrina Rasyada JMJ, Jurnal Juanda, Syahreza Hadi Juniati, Rianita Kusdiyah, Erni Lestari, Nur Afni Lisa, Mei Mara Imam Taufik Siregar Maria Estela Karolina Maya Arini Hestiningtyas MIFTAHUL JANNAH Miftahurrahma Miranda, Melly Mirna Iskandar Natasia Ulfa, Regina Nindya Aryanty Nur Afni Lestari NURIYAH Nuriyah Nuriyah Nuriyah, Nuriyah Nyimas Natasha Ayu Shafira Patrick William Gading Permana, Oki Putri Sari Wulandari Quzwain, Fairuz Chatib Resty Hardianty Lestari Rhonaz Putra Agung Rita Halim Samsirun H Samsirun Halim Simanjuntak, Charles Apul Siregar, Mara Imam Taufiq Siska, meliana Sotianingsih Sotianingsih Sotianingsih, Sotianingsih Suzan, Raihanah Syofira, Novianti Tengku Arief Buana Perkasa Wahyu Indah Dewi Aurora Zikri, Rahmat

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