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All Journal Jurnal Biologi Tropis Dinamisia: Jurnal Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education
Mulyani, Putri Dwi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Social Sciences Other

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Lignolytic Enzyme Activity of Isolated Bacteria from Termite (Coptotermes Sp.) and Milkfish (Chanos chanos Forsskal, 1775) Guts Latifah, Emi; Mulyani, Putri Dwi; Purwestri, Yekti Asih
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.19333

Abstract

Bacteria BSR 2, Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), Brevibacillus sp. (BSR 9), isolated from termite gut and Bacillus licheniformis (BSA B1) isolated from milkfish gut have been known to possess celluloytic activity. However, their lignolytic ability has not been known. This study aimed to determine the lignolytic ability of bacteria isolated from termit (Coptotermes sp.) and milkfish (Chanos chanos Forsskal, 1775) guts and their enzymes characterization. The qualitative test was done through the spot test method, while quantitative assay was performed spectrophotometrically at 335 nm to calculate vanillin concentration. The isolates were grown in Lignin Mineral Medium, then the optical density (OD620) were measured every 24 hours for 5 days using spectrophotometer to determine their growth profile and the best isolation time of the lignolytic enzyme. Based on results, the best lignolytic enzyme isolation time for strains Bacillus licheniformis (BSA B1) and BSR 2 were 5 days, yielding lignolytic enzyme activity of 0.961 ± 0.168 U/mg and 2.176 ± 0.088 U/mg respectively,  while strains Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), and Brevibacillus sp. (BSR 9) were 4 days, yielding of 1.206 ± 0.045 U/mg, 1.162 ± 0.191 U/mg, and 0.896 ± 0.108 U/mg, respectively. The strain BSR 2 showed the highest lignolytic activity compared to other strains. The optimum temperature for lignolytic enzyme activity of BSR 2 was 30 ℃ and the optimum pH was 7. The lignolytic enzyme activity showed that these bacterial isolates can be a chance to be used as new alternative lignolytic enzyme source in commercial bioconversion process.
Potential of Trichoderma harzianum as Environmentally Friendly Natural Pesticides for Controlling Phytophthora in Duku Plants Abdilla, Triyuni Verza; Simamora, Edenia Septefine; Amelia, Zahra Dini; Mulyani, Putri Dwi
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8611

Abstract

Duku fruit production in Jambi has begun to decline, one of the causes is stem cancer. This stem cancer disease is caused by the fungus Phytophthora sp. The symptoms caused by this disease are dry and soft bark like cork, wilted leaves and falling off until the tree finally dies. The purpose of this study was to isolate Phytophthora sp., characterize the morphology of the pathogen and test the ability of Trichoderma to inhibit the growth of Phytophthora sp. The research was conducted by calculating the percentage of inhibition starting from sampling the organs of the duku plant and continuing to the isolation stage. The antagonistic test of Phytophthora sp. against Trichoderma was carried out using the dual culture method (dual culture). The results showed that Phytophthora sp. can be obtained from the bark of plants, with characteristics of colonies that are textured like cotton, white in color, round in shape with uneven edges. Microscopic observation of fungal isolates showed that Phytophthora sp. has morphological characteristics in the form of round-oval conidia, non-septate mycelium and branched conidiophores. Trichoderma has the ability to inhibit the growth of Phytophthora sp. with an inhibitory power of 63.52%.
Training on the Production of Biowash Derak at SMAN 3 Muaro Jambi : Pelatihan Pembuatan Biowash Derak (Detergen Organik Berbahan Dasar Lerak) di SMAN 3 Muaro Jambi Destiana, Anggari Linda; Nugraha, Anggit Prima; Husnudin, Uni Baroroh; Maritsa, Hasna Ul; Mulyani, Putri Dwi; Yusuf, Ashif Irvan
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 9 No. 1 (2025): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v9i1.25254

Abstract

The lerak fruit (Sapindus rarak) possesses significant potential as a natural detergent that can serve as an alternative to chemical-based detergents, which are increasingly concerning due to their detrimental effects on the environment and their impact on soil and aquatic organisms. The saponin content in lerak generates foam and exhibits antibacterial properties, making it environmentally safe for both soil and aquatic life. The objective of this program is to train students and teachers at SMAN 3 Muaro Jambi in producing natural detergent from lerak, thereby supporting extracurricular batik activities while also contributing to the establishment of an environmentally friendly school. The stages of this community service activity include socialization, hands-on training, and evaluation (pre- and post-tests). The results of the satisfaction measurement indicate that all participants were satisfied with the implementation of this community service, with 44.4% expressing high satisfaction and 55.6% being satisfied. The percentage of participant's knowledge regarding the name, benefits, preparation methods, and usage of lerak increased by 86%. The number of participants capable of producing lerak detergent rose significantly, from 4.7% to 85.4%, representing an increase of 80.7%. Overall, all participants expressed satisfaction with this activity and demonstrated an enhancement in their knowledge.
Co-Authors Abdilla, Triyuni Verza Amelia, Zahra Dini Anggari Linda Destiana Hasnaul Maritsa Latifah, Emi Nugraha, A. Prima Simamora, Edenia Septefine Uni Baroroh Husnudin Yekti Asih Purwestri Yusuf, Ashif Irvan