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Hubungan Ekspresi Interleukin-17 dan Indeks Proliferasi Ki67 pada Karsinoma Payudara Ika Kartika,; Theodorus; Citra Dewi; Nyiayu Fauziah Kurniawati
Majalah Patologi Indonesia Vol 30 No 1 (2021): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundTumor cells have the ability to change the phenotype of inflammatory cells in the tumor environment into protumoral factors. One ofthe mediators produced such as interleukin 17 (IL-17) also plays a role in increasing tumor proliferation and invasion. Similar to IL-17,Ki67 has generally been used as a marker to assess the extent of breast carcinoma proliferation. This is the first study to determinethe relationship of IL-17 expression with Ki67 proliferation index of breast carcinoma patients.MethodsThis is a case series study using 38 paraffin block samples of breast carcinoma patients. The clinicopathological characteristicsdocumented are age, histopathological subtype and grade, TILs (tumor infiltrating lymphocytes) and molecular subtypes. Interleukin17 and Ki67 expressions were assessed using immunohistochemistry examination and data were analysed using the Pearson andSpearman test to assess correlations between the two variables.ResultsGeneral characteristics of the sample in this study were breast cancer patients aged >40 years (73.7%), histopathological subtypesof non-specific invasive carcinoma (84.2%), high tumor rates (78.9%), non-dominant TILs (94.7%) and molecular subtype luminal B(55.3%). There is no relationship between IL-17 expression and Ki67 proliferation index (p 0.72 and OR of 1.417), even though lowerexpression of IL-17 showed a higher Ki67 proliferation index.ConclusionExpression of IL-17 cannot describe the Ki67 proliferation index in breast carcinoma. Studies using another analytical techniques andlarge sample size are suggested to obtain more accurate results.

Analisis Ekspresi Interleukin-17 pada Karakteristik Klinikopatologi Karsinoma Payudara Nyiayu Fauziah Kurniawati; Ika Kartika; Citra Dewi; Theodorus
Majalah Patologi Indonesia Vol 30 No 1 (2021): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundInteraction between cancer cells and tumor microenvironment plays roles in cancer proliferation and invasiveness. Many type ofcytokine produced by inflammatory cells in tumor stroma could stimulate tumor proliferation and invasiveness, such as interleukin-17.The aim of this study is to determine the correlation between IL-17 expression and clinicopathological characteristics of breast cancer.MethodsSerial cases study was performed by enrolling 40 paraffin block of breast cancer patients. Clinicopathological characteirstics werenoted, including age, histopathological subtype and grade, TILs, lymphovascular invasion and molecular subtype. TILs were scoredby denomination area of stromal tissue occupied by mononuclear cells over total stromal area. Expression of IL-17 was examined byusing immunohistochemistry with anti-IL-17 antibody and counted from stromal mononuclear cells cytoplasm staining from five areas.The average value was used as a cut-off point in determining level of expression. Data were analysed by using SPSS v.22.ResultsMajority of the patients were more than 40 years old (75%), histopathological grade III (77.5%), without lymphovascular invasion(62.5%), luminal B molecular subtype (57.5%), and non-dominant TILs (95%). IL-17 expression was not significantly correlated withclinicopathological characteristics with p value=0.079, 0.600, 0.739, 0.883 and 1.000 respectively for age, histological grade,lymphovascular invasion, molecular subtype and TILs.ConclusionInterleukin-17 expression and clinicopathological on characteristics of breast carcinoma between histopathological grade, TILs, andlymphovascular invasion showed no significant differences. There was a tendency that the higher IL-17 expression, the higherhistopathological grade, the lower TILs and positive lymphovascular invasion

Mastitis Granulomatosa: Sebuah Tantangan Diagnosis Citra Dewi; Ella Amalia; Nyiayu Fauziah Kurniawati; Maria Ulfa, Maria Ulfa,; Eka Putra Pratama; Krisna Murti
Majalah Patologi Indonesia Vol 31 No 1 (2022): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundGranulomatous mastitis (GM) is often found in reproductive-age women. These patients are treated by the administration ofantituberculosis drugs. It is difficult to determine the exact cause of GM cytologically and histopathologically. Various etiologies i.e.,tuberculosis, sarcoidosis, foreign bodies and immunological diseases may provide granulomatous inflammation features in thetissue. High-sensitivity tests such as PCR can help to detect the presence of Mycobacterium tuberculosis (MT) as one of possibleetiologic agent. Accurate diagnosis will improve the treatment quality thus a better quality of life. The aim of this study was todetermine whether the granulomatous mastitis patients who referred to the Department of Anatomic Pathology Faculty of MedicineUniversitas Sriwijaya/Dr. Moh. Hoesin Palembang Hospital were caused by MT.MethodsA descriptive observational study was performed. Thirty paraffin blocks of GM patients during the period of 1st October 2018 to 29thSeptember 2020 were collected. DNAs were extracted from tissue scrapings of the slides. Then, PCR analysis was conducted usinga specific primer IS6110 with around 200 bp PCR product.ResultsThe mean age of the patients was 40.8 years (range: 23-67 years). There were six samples (20%) of GM produced the expectedamplicon. However, only three of six samples morphologically consistent with tuberculosis.ConclusionGranulomatous inflammatory reactions in the breast are not only caused by tuberculosis. Further study is needed to determine theexact cause of this lesion. Despite high costs, PCR examination may become an alternative approach in determining the etiology ofGM.

PEMANFAATAN EKSTRAK BUNGA ASOKA SEBAGAI ALTERNATIF PENGGANTI EOSIN PADA PEWARNAAN HEMATOXYLIN EOSIN Haliza, Nur; Sari, Indah; Hartati, Dewi; Kurniawati, Nyiayu Fauziah
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 17 No 1 (2025): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v17i1.2707

Pembuatan preparat jaringan menggunakan pewarnaan hematoksilin eosin. Harga reagensia yang digunakan relatif mahal sehingga berpotensi untuk pemanfaatan bahan baku lokal dalam menggantikan pewarnaan eosin. Bunga Asoka merah (Ixora coccinea) mengandung glikosida antosianin berwarna merah yang dapat digunakan sebagai alternatif pengganti eosin. Tujuan penelitian untuk memanfaatkan ekstrak bunga asoka yang ramah lingkungan sebagai pengganti eosin dalam pewarnaan hematokxylin eosin. Desain penelitian yang digunakan adalah true experimen design. Teknik sampling pada penelitian menggunakan Nonprobability dalam bentuk Purprosive sampling. Sampel berupa jaringan payudara (mammae) manusia berjumlah 32 preparat jaringan untuk melihat hasil pemeriksaan menggunakan alternatif bunga asoka sebanyak 16 preparat dan eosin sebagai kontrol sebanyak 16 preparat. Data hasil pemeriksaan akan diolah secara analisis deskriptif berdasarkan hasil skor penilaian preparat sediaan jaringan. Pada kontrol pewarnaan hematoxylin eosin 100% menunjukkan kualitas sediaan yang sangat baik dengan skor adalah (4), menghasilkan warna biru pada inti sel sangat jelas, warna merah muda sangat jelas, dan dapat didiagnosis, sedangkan untuk pewarnaan esktrak bunga asoka 93,8% menunjukkan kualitas sediaan kurang baik dengan skor adalah (2) dan (1), menghasilkan biru pada inti sel, kurang warna merah muda tetapi masih dapat didiagnosis. Hasil uji Chi-Square diperoleh p-value 0.000<0.05 menunjukan bahwa terdapat perbedaan signifikan dari kedua proses pewarnaan menggunakan ekstrak bunga asoka dengan eosin.

Mastitis Granulomatosa: Sebuah Tantangan Diagnosis Dewi, Citra; Amalia, Ella; Kurniawati, Nyiayu Fauziah; Maria Ulfa,, Maria Ulfa,; Pratama, Eka Putra; Murti, Krisna
Majalah Patologi Indonesia Vol. 31 No. 1, Januari 2022
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v31i1.492

BackgroundGranulomatous mastitis (GM) is often found in reproductive-age women. These patients are treated by the administration ofantituberculosis drugs. It is difficult to determine the exact cause of GM cytologically and histopathologically. Various etiologies i.e.,tuberculosis, sarcoidosis, foreign bodies and immunological diseases may provide granulomatous inflammation features in thetissue. High-sensitivity tests such as PCR can help to detect the presence of Mycobacterium tuberculosis (MT) as one of possibleetiologic agent. Accurate diagnosis will improve the treatment quality thus a better quality of life. The aim of this study was todetermine whether the granulomatous mastitis patients who referred to the Department of Anatomic Pathology Faculty of MedicineUniversitas Sriwijaya/Dr. Moh. Hoesin Palembang Hospital were caused by MT.MethodsA descriptive observational study was performed. Thirty paraffin blocks of GM patients during the period of 1st October 2018 to 29thSeptember 2020 were collected. DNAs were extracted from tissue scrapings of the slides. Then, PCR analysis was conducted usinga specific primer IS6110 with around 200 bp PCR product.ResultsThe mean age of the patients was 40.8 years (range: 23-67 years). There were six samples (20%) of GM produced the expectedamplicon. However, only three of six samples morphologically consistent with tuberculosis.ConclusionGranulomatous inflammatory reactions in the breast are not only caused by tuberculosis. Further study is needed to determine theexact cause of this lesion. Despite high costs, PCR examination may become an alternative approach in determining the etiology ofGM.

PKM Bantuan Hidup Dasar (BHD) untuk meningkatkan pengetahuan menolong korban henti jantung di Pondok Pesantren Modern IGM AL-Ihsaniyah Palembang. Mastan, Soraya Sagita Desmaradd; Rizki, Aulia Annisa; Kurniawati, Nyiayu Fauziah; Oktari, Desyani; Ramkita, Nora
Jurnal Abdimas Mandiri Vol. 10 No. 1 (2026): On Progress
Publisher : UNIVERSITAS INDO GLOBAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36982/jam.v10i1.6817

Henti jantung merupakan kondisi kegawatdaruratan medis yang dapat menyebabkan kematian dalam waktu singkat apabila tidak segera ditangani. Tindakan Bantuan Hidup Dasar (BHD) terbukti mampu meningkatkan peluang keselamatan korban henti jantung, namun pengetahuan dan keterampilan masyarakat awam, termasuk santri di lingkungan pondok pesantren, masih terbatas. Kegiatan Pengabdian kepada Masyarakat ini dilaksanakan sebagai bagian dari peringatan International Pathology Day dengan tujuan meningkatkan pengetahuan dan keterampilan santri, ustad, dan ustadzah mengenai Bantuan Hidup Dasar sebagai upaya meningkatkan kesiapsiagaan dalam menolong korban henti jantung di Pondok Pesantren Modern IGM Al-Ihsaniyah Palembang. Metode pelaksanaan kegiatan meliputi edukasi kesehatan melalui ceramah interaktif, demonstrasi audiovisual, serta praktik langsung Bantuan Hidup Dasar menggunakan manekin. Evaluasi keberhasilan kegiatan dilakukan menggunakan kuesioner pre-test dan post-test untuk menilai tingkat pengetahuan peserta sebelum dan sesudah dilakukannya edukasi. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan yang signifikan, ditandai dengan peningkatan kategori pengetahuan baik dari 14,7% sebelum edukasi menjadi 66,2% setelah edukasi, serta penurunan kategori pengetahuan kurang dari 58,8% menjadi 4,4%. Temuan ini menunjukkan bahwa edukasi Bantuan Hidup Dasar yang disertai demonstrasi dan praktik langsung efektif dalam meningkatkan pemahaman dan kesiapsiagaan peserta. Kegiatan ini dapat berkontribusi dalam meningkatkan kemampuan masyarakat pondok pesantren dalam memberikan pertolongan awal pada kejadian henti jantung sebelum bantuan medis lanjutan tiba.

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