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Infektivitas Cendawan Entomopatogen Lecanicillium lecanii Terhadap Mortalitas Hama Penggerek Ubi Jalar Cylas formicarius (Fabricius) Dewi, Putri Kemala; Afifah, Lutfi; Surjana, Tatang; Kurniati, Anik
JURNAL AGROPLASMA Vol 9, No 2 (2022): AGROPLASMA VOL 9 NO 2
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v9i2.3177

Salah satu faktor yang mempengaruhi dalam penurunan hasil dan kualitas ubi jalar adalah serangan hama Cylas formicarius. Cendawan entomopatogen Lecanicillium lecanii merupakan salah satu agens hayati yang dapat digunakan untuk mengendalikan berbagai jenis serangga hama. Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi cendawan entomopotagen L. lecanii untuk mengendalikan imago C. formicarius. Metode yang digunakan adalah metode eksperimen dan rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) faktor tunggal yang terdiri dari 4 perlakuan dalam 6 kali ulangan : Kontrol (Aquades); 106 konidia/ml; 107 konidia/ml; 108 konidia/ml. Pengamatan dilakukan setiap hari selama 10 hari setelah aplikasi (hsa) dengan menghitung 10 imago C. formicarius yang mati akibat disemprot dengan suspensi cendawan L. lecanii sebanyak 1 ml/perlakuan. Hasil penelitian menunjukkan bahwa kerapatan konidia L. lecanii 108 konida/ml memberikan hasil paling efektif terhadap mortalitas C. formicarius sebesar 50% dengan nilai LT50 sebesar 10,024 hari. Dengan demikian, cendawan entomopatogen L. lecanii sebagai agens hayati C. formicarius layak untuk dikembangkan lebih lanjut.

TOXICITY TEST OF Annona muricata L. LEAF EXTRACT AS A BIOPESTICIDE AGAINST MORTALITY OF Spodoptera frugiperda J. E. Smith Khoirunnisya, Hylda Aulia; Afifah, Lutfi; Surjana, Tatang; Yustiano, Anton
JURNAL AGROPLASMA Vol 9, No 2 (2022): AGROPLASMA VOL 9 NO 2
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v9i2.3160

Spodoptera frugiperda is a new invasive pest that can cause losses in corn production, so control measures are needed to suppress its population. The purpose of this study was to obtain the best concentration of botanical pesticide from soursop leaves extract against the fall armyworm (S. frugiperda). The method used is experimental method using a single factor Completed Randomized Design (RAL) consisting of 7 treatments with 4 replications: A (Control); B (Insecticide deltamethrin 1 ml/lt); C (A. muricata leaves extract 20 gr/lt); D (A. muricata leaves extract 30 gr/lt); E (A. muricata leaves extract 40 gr/lt); F (A. muricata leaves extract 50 gr/lt); G (A. muricata leaves extract 60 gr/lt). The treatment effect was analyzed with Analysis of Variance (ANOVA). If the 5% level F test showed significant results, then it proceeded with the DMRT (Duncan Multiple Range Test) further test at a 5% significance level to determine the best treatment. The results achieved from this study were the concentration of soursop leaves extract 60 gr/lt was able to cause 50% mortality of S. frugiperda in less than one day based on the analysis of probit LT50, inhibiting the larvae from stopping eating with a percentage of 75% at 1 Day After Application (DAA), gave the highest mortality of 85% in 3 DAA. A. muricata leaves extract with a concentration of 60 g/lt was recommended to be used because it gave the best results that were not significantly different from the use of synthetic insecticides against S. frugiperda.

Pengaruh Kombinasi Dosis Pupuk Kandang Kotoran Burung Puyuh dan NPK Terhadap Pertumbuhan dan Hasil Tanaman Sawi Hijau (Brassica juncea L.) Varietas Tosakan F1 Rahmah, Alfi Khalifah; Muharam, Muharam; Surjana, Tatang
JURNAL AGROPLASMA Vol 10, No 1 (2023): JURNAL AGROPLASMA VOLUME 10 NO 1 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i1.3680

Green mustard (Brassica juncea L.) is a vegetable plant that is much needed by the community. One of the factors causing the decline in mustard greens production is due to the community's need for mustard greens which is quite high along with population growth which continues to grow and the harvested area for mustard greens is decreasing. The development of mustard greens produced generally still uses inorganic fertilizers so that it is not yet oriented towards organic products or manure. Quail droppings can cause environmental pollution so that it becomes a solution to be used as manure. This study aims to obtain a combination of doses of manure, quail manure and NPK which gives the highest yield for the growth and yield of mustard greens (Brassica juncea L.) Tosakan F1 variety. The research was carried out in an experimental field located at Jalan DarmaBakti, Curug Village, Kiara Residence, West Bogor District, Bogor City. The research site is located at an altitude of 195 meters above sea level. The research took place from December 2021 to February 2022. The research method used was a single factor Randomized Group Design (RBD) consisting of 9 treatments and 3 replicates: A (Control), B (15 tons/ha of manure), C (250 kg /ha NPK), D (5 tons/ha manure + 200 kg/ha NPK), E (10 tons/ha manure + 200 kg/ha NPK), F (15 tons/ha manure + 200 kg/ha NPK), G (5 tonnes/ha of manure + 150 kg/ha of NPK), H (10 tonnes/ha of manure + 150 kg/ha of NPK) and I (15 tonnes/ha of manure + 150 kg/ha of NPK). Data were analyzed using the F-test analysis at the 5% level if there was a significant effect followed by the DMRT (Duncan Multiple Range Test) advanced test at the 5% level. The results of this study showed no significant effect of giving a combination dose of quail manure and NPK on the growth and yield of mustard greens (Brassica juncea L.) of the Tosakan F1 variety.Â Keywords: Mustard green variety Tosakan F1, quail manure fertilizer, NPK fertilizer.

Uji Daya Hasil Lanjutan Galur-Galur Padi (Oryza sativa L) Sawah Irigasi Berpotensi Hasil Tinggi Aulia, Gebby Reza; Afifah, Lutfi; Surjana, Tatang; Pramudyawardani, Estria Furry; Dewi, Ratna Sari; Roza, Celvia
Indonesian Journal of Agrotech Vol. 8 No. 1 (2023): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture Universitas Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33661/jai.v8i1.6344

The continual of the same high yield variety (HYV) is not recommended because it can cause resistance breakage against pest disease. One of the Indonesian Center for Rice Research (ICRR) main tasks is to establish new rice varieties with high yield potential and other better characteristics than the previous HYVs. Yield Test of promising lines aimed to select the yield potential, resistance to major pests, and rice quality to be further tested in a breeding processes. The research was carried out in the ICRR Experimental station, located in Sukamandijaya Village, Subang Regency, West Java. This study used a randomized complete block design with 4 replications consisting of 14 advanced generation lines, namely G1 (BP 30533D-SKI-4-3-1), G2 (BP 19210e-6-2-1- 3), G3 (BP 30531D-SKI-24-3), G4 (BP 30531D-SKI-1-2-3), G5 (BP 30534D-SKI-6-1), G6 (BP 30533D-SKI-4- 3-2), G7 (BP 30485E-SKI-9-5-2-0), G8 (BP 30533D-SKI-29-1-1-Ski), G9 (BP 31507D-SKI-1-1-1) , G10 (BP 30475c-SKI-6-4-1-5), G11 (Koshi CSSL [IR64] SL2129), G12 (BP 30533D-SKI-29-1-1-Skb), G13 (BP 31456D-SKI- 7-1), G14 (BP 31523D-SKI-2-1-0-0), and 2 checks (A (INPARI 33) and B (INPARI 32)). The results of the analysis of variance and the post hoc test used was DMRT test The data were analyzed by analysis of variance on yield did not show a significant difference between the lines tested and the best checks INPARI 33 ( 6.18 t/ha MPD). The G1 and G11 lines showed a moderately resistant response to bacterial leaf blight (HDB) IV, both lines were recommended for further testing

Production of The Entomopathogenic Fungi Beauveria bassiana using Starch Rich Substrate and Its Infectivity against Tribolium castaneum Afifah, Lutfi; Afifah, Dhiya Mutsla; Surjana, Tatang; Kurniati, Anik; Maryana, Rosalia
Jurnal ILMU DASAR Vol 23 No 2 (2022)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v23i2.21435

The entomopathogenic fungi of Beauveria bassiana is known to infect the Tribolium castaneum pest. Mass production of B. bassiana is currently carried out using alternative media, the amount of conidia produced on alternative media is quite large and can tolerate temperature. This research aims to select alternative media for the propagation of B. bassiana to gain germination, conidia density, and virulence of the best alternative media concentration against the pest of T. castaneum. The method used was a compiled in the Completely Randomized Design (RAL) at all stages of research. In the first stage of alternative media selection consisting of 4 treatments in 6 replications: A (PDA); B (peanuts); C (soybean); D (old coconut meat). The second stage of alternative media infectivity consisting of 5 treatments in 5 replications: positive control (synthesis insecticide); negative control (aquades); 107; 108; 109 spores/ml. The results achieved that alternative media selection given a highest colony diameter is 4.39 cm on soybean, the highest conidia density is 5,61 x 108 spores/ml on peanuts, the highest germinating power is 31.76% on peanuts. Furthermore, the infectivity of alternative media obtained on peanut media had a significant effect on the mortality of T. castaneum (36.00%) at a concentration of 109 spores/ml, LC50 was obtained at 5.6 x 105 spores/ml, and LT50 within 5.42 days. The results indicated that the peanut media was a good propagation medium of B. bassiana and it was necessary to find other alternatives to increase the infectivity of B. bassiana against T. castaneum.

Respon Galur Jagung Manis (Zea mays Var. saccharata Sturt) MS-UNSIKA Generasi M8 Terhadap Hama Ulat Grayak (Spodoptera frugiperda) di Dataran Rendah Shadrina, Wahdani Nur; Syafii, Muhammad; Surjana, Tatang
JURNAL AGROPLASMA Vol 11, No 2 (2024): JURNAL AGROPLASMA VOLUME 11 NO 2 TAHUN 2024
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v11i2.5942

Seeds with superior quality can reduce the level of disease and pest attacks and can increase production yields. This research aims to determine differences in resistance of several lines of sweet corn (Zea Mays Var. saccharata Sturt) based on armyworm pest resistance. This research was carried out in one of the lands of Kalihurip Village, Cikampek District, Karawang Regency. The experiment will start from February 2024 to May 2024. The method used is experimental, using a single factor Randomized Block Design (RAK) consisting of 10 treatments and repeated 3 times, so that 30 experimental units are obtained. consisting of: SR1 (MS-UNSIKA- profilik), SR2 (SR-3 Sweet Boy F2), SR3 (SR-1 Latan), M6 (M6 gamma ray radiation dose 200 gy), M9 (M9 gamma ray radiation dose 200 gy ), M10 (M10 gamma ray radiation 200 gy), G11 (F2 mutant Talent variety), G12 (S1 Maestro variety), G13 (F2 Talent variety), G14 (F2 Bonanza variety). The experimental results will be tested using the F test, if there is a real effect then it will be tested further by the Duncan Multiple Range Test (DMRT) at 5% level. The results of the research showed that there was no real difference in the intensity of armyworm attacks on various lines of sweet corn plants in the treatment of various lines of sweet corn on the intensity of armyworm attacks. The Ms-Unsika strain showed the highest resistance, while Var. Maestro showed the highest susceptibility and there was no real correlation between disease and pest attacks on production results in the parent line of sweet corn (Zea mays Var. saccharata Sturt) MS-Unsika generation M8 mutant. Keywords: sweet corn parent lines, pests, crop yield

Intensitas Serangan Penyakit Penting pada Beberapa Galur Jagung Manis (Zea mays Var. saccharata Sturt) MS-UNSIKA Generasi M8 Sulastri, Sindy; Surjana, Tatang; Syafi'i, Muhammad
JURNAL AGROPLASMA Vol 11, No 2 (2024): JURNAL AGROPLASMA VOLUME 11 NO 2 TAHUN 2024
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v11i2.5944

Sweet corn is a holticultural that has an important role as a national food source. Corn serves as an alternative food after rice that is commonly consumed by people in Indonesia. One of the causes of decreased sweet corn productivity is attack on corn plant disease. Some important disease that attack sweet corn is leaf blight and leaf rust. Use a genetically mutated varieties resistant to these diseases is one of solution to increase sweet corn production caused by disease attack. This study aims to obtain the lowest intensity of important disease and the best production result in the MS-UNSIKA generation M8 sweet corn. The research was conducted in one of the lands of Kalihurip Village, Cikampek sub District, Karawang District, from February 2024 to Mei 2024. The research method used was an experimental method, using a Single Factor Randomized Block Design (RAK) consisting of 6 MS-UNSIKA M8 sweet corn lines and 4 comparison varieties which were repeated 3 times in plots measuring 2 m x 2 m.. The results of the study showed significantly different effects of leaf blight attacks at 42 and 49 HST, and leaf rust disease at 56 and 63 HST. The Maestro and Bonanza varieties are the varieties that have the lowest attacks of leaf blight and rust with an average attack intensity of21.40% and 11.08% respectively. The results of this research also show that there is no correlation between leaf blight attacks on the production of sweet corn lines (Zea mays Var. saccharata Sturt). Keywords: sweet corn, leaf blight, leaf rust, important diseease

Uji Efektivitas Beberapa Media Tumbuh Alternatif Limbah Organik dengan Penambahan Tepung Kulit Udang terhadap Pertumbuhan Cendawan Entomopatogen Lecanicillium lecanii Mubarokah, Annafs Zakiyyah; Afifah, Lutfi; Surjana, Tatang; Kurniati, Anik
Paspalum: Jurnal Ilmiah Pertanian Vol. 12 No. 2 (2024)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35138/paspalum.v12i2.756

Alternative control uses the entomopathogenic fungus Lecanicillium lecanii which is propagated using agro-industrial waste as a substrate for the production of locally available conidia. Propagation of L. lecanii was carried out on media supplemented with shrimp shell flour as additional nutrition. This research aims to provide the best alternative media for the growth of L. lecanii. The research was carried out at the Biological Agents Laboratory (BBPOPT), from March to May 2024. The method used was an experiment with a single factor Completely Randomized Design (CRD). This research consisted of 7 treatments in 4 repetitions: K (Corn Media); A (Sugar Bagasse); B (Tea Dregs); C (Sawdust); D (Sugar Bagasse + Shrimp Shell Flour); E (Tea Dregs + Shrimp Shell Flour); F (Sawdust + Shrimp Shell Flour). The results showed that L. Lecanii has a short incubation period, namely one hsi and alternative organic waste media with the addition of shrimp shell flour is more effectively used in the propagation of the L. lecanii fungus and has a significant effect on colony diameter (8.84 cm) in sawdust + shrimp shell flour. Alternative media with the addition of shrimp shell flour is recommended for the growth of the fungus L. lecanii.

PENGARUH PENAMBAHAN TEPUNG KULIT UDANG PADA MEDIA LIMBAH ORGANIK TERHADAP PERTUMBUHAN CENDAWAN Beauveria bassiana Adelita, Shafa; Afifah, Lutfi; Surjana, Tatang
Jurnal Ilmiah Hijau Cendekia Vol 10 No 2 (2025): Jurnal Ilmiah Hijau Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/hijau.v10i2.5581

Beauveria bassiana is one of the biological agents that has a huge potential to control insect pests, the mass production has to be developed in a large scale. In the process of micropropagation, the culture media should contain appropriate and contain nutrition that can produce optimal growth. This study aimed to determine the effect of adding shrimp shell powder to support the growth of B. bassiana. The study was designed using a single factor Completely Randomized Design (CRD) at each stage of research. This stage consists of 7 treatments in repeated 4 times: K (control corn media); A (Sugarcane bagasse); B (Tea dregs); C (Sawdust); D (Sugarcane bagasse + Shrimp shell powder); E (Tea dregs + Shrimp shell powder); F (Sawdust + Shrimp shell powder). The data obtained and analyzed using one way analysis of variant (ANOVA). The results showed that the addition of chitin powder was not significantly effective for the diameter of B. bassiana, but the incubation period was 2 days after inoculation and the diameter given 4,03 cm up to 8,8 cm in general. The addition of chitin powder gave the best physical appearance of B. bassiana.

Karakteristik Biologi dan Preferensi Pakan Spodoptera frugiperda (Lepidoptera: Noctuidae) pada Berbagai Jenis Pakan Hidayah, Vania Utami; Afifah, Lutfi; Surjana, Tatang; Subagyo, Vani Nur Oktaviany
AGRICA Vol. 17 No. 1 (2024): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v17i1.3437

Fall Armyworm (Spodoptera frugiperda) is one of the invasive insect species that cause yield loss in corn crops. This pest can attack other crops, such as food crops, vegetables, legumes, and weeds. This research aims to determine the host suitability of S. frugiperda on several types of host plants. The method used was an experimental method with a single-factor completely randomized design (CRD) consisting of four treatments in five replications using four different types of host plants: maize (Zea mays L.), soybean (Glycine max L.), papaya (Carica papaya L.), and chinese kale (Brassica oleraceae var. acephala). The highest percentage of S. frugiperda attack intensity in the choice test was obtained in the treatment with chinese kale leaves (70%) and the lowest in the papaya leaves treatment (20%). In the feeding activity of 3rd instar larvae, the treatment of maize leaves and soybean leaves showed the fastest feeding activity at 11:00 and 12:00, while in the feeding activity of 4th instar larvae, the treatment of maize leaves and chinese kale leaves showed a fast larval feeding activity with a feeding duration of ± 5-10 minutes. Fall Armyworm can also attack other host plants such as soybeans and chinese kale. It can even endure by feeding on papaya leaves, which are typically used as a botanical pesticide. It is essential to step up surveillance against this pest because there are so many additional possible hosts for it.

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