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Lutfi Afifah, Lutfi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Industrial & Manufacturing Engineering Mathematics Social Sciences

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INVEKTIVITAS CENDAWAN ENTOMOPATOGEN Beauveria bassiana UNTUK MENGENDALIKAN HAMA BOLENG Cylas formicarius F Amelia, Nada; Afifah, Lutfi; Sugiarto; Kurniati, Anik
Jurnal Agrotech Vol 13 No 1 (2023)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/agrotech.v13i1.108

Abstract

Hama boleng (Cylas formicarius) merupakan hama utama pada tanaman ubi jalar dan dapat menurunkan hasil sebanyak 100%. Beauveria bassiana merupakan cendawan entomopatogen yang berpotensi untuk mengendalikan hama tanaman. Efikasi B. bassiana dipengaruhi oleh produksi toksin yang terdiri dari beauvericin, bassianin, bassiacridin, bassianolide, cyclosporine, oosporein, dan tenellin yang dapat mengganggu sistem syaraf dan membunuh serangga sasaran. Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi cendawan entomopatogen B. bassiana untuk mengendalikan imago C. formicarius. Metode penelitian yang digunakan adalah rancangan acak lengkap (RAL). Terdapat 4 (empat) perlakuan yaitu kontrol (akuades); 107; 108; 109 konidia/ml dalam 6 (enam) kali ulangan. Pengamatan dilakukan setiap hari selama 10 hari setelah aplikasi (hsa) dengan menghitung 10 imago C. formicarius yang mati akibat disemprot dengan suspensi cendawan B. bassiana sebanyak 1 ml/perlakuan. Data yang diperoleh berupa persentase mortalitas dan LT50 hama C. formicarius dan dilanjutkan dengan uji lanjut Beda Nyata Terkecil (BNT) 5% pada pengamatan mortalitas dan LT50 dengan analisis uji probit. Hasil penelitian menunjukkan bahwa kerapatan konidia B. bassiana 109 konidia/ml memberikan hasil paling efektif terhadap mortalitas C. formicarius sebesar 53% dengan nilai LT50 yang diperoleh 7,6 hari. Dengan demikian, cendawan entomopatogen B. bassiana sebagai agens hayati C. formicarius layak untuk dikembangkan.
VIRULENSI CENDAWAN ENTOMOPATOGEN Beauveria bassiana TERHADAP WERENG BATANG COKLAT Nilaparvata lugens Stal Ihsan, Alya Khairani; Afifah, Lutfi; Sugiarto; Kurniati, Anik
Jurnal Agrotech Vol 13 No 1 (2023)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/agrotech.v13i1.136

Abstract

Penurunan hasil padi akibat serangan Nilaparvata lugens Stal dapat dilakukan pengendalian dengan agens hayati untuk menghindari resistensi pada Wereng Batang Coklat (WBC). Cendawan entomopatogen Beauveria bassiana adalah salah satu Agens hayati dengan kisaran inang yang luas. Penelitian ini bertujuan untuk mengetahui virulensi cendawan entomopotagen B. bassiana terhadap pengendalian N. lugens. Penelitian ini menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL) faktor tunggal yang terdiri dari 5 perlakuan yang diulang 4 kali: Kontrol (Akuades); 106 konidia/ml; 107 konidia/ml; 108 konidia/ml; 109 konidia/ml. Pengamatan dilakukan selama 7 hari setelah aplikasi (hsa) secara berturut-turut dengan menghitung 10 nimfa instar 3 N. lugens yang mati akibat penyemprotan 1 ml/perlakuan suspensi cendawan B. bassiana. Hasil penelitian menunjukkan konsentrasi suspensi konidia B. bassiana 109 konida/ml memberikan hasil tertinggi pada mortalitas B. bassiana hingga 95% dengan nilai LC50 (5,1 x 108 konidia/ml). B. bassiana terbukti virulen terhadap N. lugens dan menyebabkan mortalitas yang tergolong tinggi B. bassiana sehingga dapat menjadi rekomendasi alternatif pengendalian wereng batang coklat.
INTENSITAS PENYAKIT TANAMAN PADI (Oryza sativa L.) VARIETAS CIHERANG PADA APLIKASI BEBERAPA TEKNIK PENGENDALIAN Sandi Ramadhan; Afifah, Lutfi; Satriyo Restu Adhi; Budi Irfan
Jurnal Agrotech Vol 13 No 2 (2023)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/agrotech.v13i2.148

Abstract

Teknik pengendalian penyakit tanaman dapat memengaruhi intensitas penyakit. Penelitian ini bertujuan mendapatkan teknik pengendalian terbaik yang dapat menekan intensitas penyakit pada tanaman padi (Oryza sativa L.) varietas Ciherang. Metode penelitian yang digunakan adalah metode eksperimen, dengan rancangan acak kelompok (RAK) faktor tunggal, terdiri dari 4 perlakuan dan 6 ulangan. Perlakuan teknik pengendalian terdiri dari kontrol (K), pengendalian kombinasi (PK), pengendalian biointensif (PB) dan pengendalian sintetis (PS). Data hasil pengamatan dianalisis dengan sidik ragam dan apabila hasilnya berbeda nyata, maka diuji lanjut dengan uji beda nyata terkecil (BNT) pada taraf 5%. Hasil penelitian menunjukkan, terdapat pengaruh nyata pada intensitas penyakit blas (Pyricularia oryzae), hawar daun bakteri (Xanthomonas oryzae), dan hawar daun pelepah (Rhizoctonia solani). Perlakuan PS memberikan hasil terendah terhadap insidensi penyakit blas (39,3-37,6%), insidensi hawar daun bakteri (15,5-54,5%), insidensi hawar daun pelepah (0,3-60,6%), severitas penyakit blas (31,1-51,1%), severitas penyakit hawar daun bakteri (14,5-39,0%), dan severitas hawar daun pelepah (0,2-25,8%).
Kelimpahan dan Keanekaragaman Arthropoda dengan Teknik Pengendalian Berbeda Pada Pertanaman Padi (Oryza sativa L.) Varietas Ciherang Taufiqullathif, Muhammad Hammad; Afifah, Lutfi; Saputro, Nurcahyo Widyodaru; Irfan, Budi
JURNAL AGROPLASMA Vol 10, No 2 (2023): JURNAL AGROPLASMA VOLUME 10 NO 2 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i2.4819

Abstract

Arthropods Abundance and Diversity Using Different Pest Control on Paddy Cultivation (Oryza sativa L.) Ciherang Varieties. Efforts to increase and maintain rice production face challenges due to attacks by arthropod pests. The most widely applied pest control by farmers is by using synthetic insecticides. However, synthetic insecticides affect the abundance of arthropods and are bad for the environment. This study aimed to obtain the lowest pest control technique on the abundance of arthropod pests, the best for natural enemiesand arthropod diversity, dominance, and evenness. The experiment design was Randomized Block Design (RBD) with 6 replications and 4 treatments including Untreated (UT), Combination Treatment (CT), Biointensive Treatment (BT), and Synthetic Treatment (St). Arthropod samples were taken 10 times from 1 – 10 WAP using 3 traps namely yellow sticky trap, sweep net, and vacuum trap. The data were analyzed using variance, and if the results were significantly different, it was followed by the Least Significant Difference (LSD) at 5% level. The results of the analysis showed that ST gave the lowest abundance to the average abundance (741,83), yellow sticky trap (337.67) and sweep net (323.00) significantly different from other treatments. But was not significanty diffirent to the vacuum trap (81.17). The index values of diversity, dominance, and evenness for each role of arthropods are classified as medium, low, and medium. This indicates that the agroecosystem is in a state of balance. Keywords:Abundance, Diversity, Biointensive treatment,IPM, Arthropods
Infektivitas Cendawan Entomopatogen Lecanicillium lecanii Terhadap Mortalitas Hama Penggerek Ubi Jalar Cylas formicarius (Fabricius) Dewi, Putri Kemala; Afifah, Lutfi; Surjana, Tatang; Kurniati, Anik
JURNAL AGROPLASMA Vol 9, No 2 (2022): AGROPLASMA VOL 9 NO 2
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v9i2.3177

Abstract

Salah satu faktor yang mempengaruhi dalam penurunan hasil dan kualitas ubi jalar adalah serangan hama Cylas formicarius. Cendawan entomopatogen Lecanicillium lecanii merupakan salah satu agens hayati yang dapat digunakan untuk mengendalikan berbagai jenis serangga hama. Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi cendawan entomopotagen L. lecanii untuk mengendalikan imago C. formicarius. Metode yang digunakan adalah metode eksperimen dan rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) faktor tunggal yang terdiri dari 4 perlakuan dalam 6 kali ulangan : Kontrol (Aquades); 106 konidia/ml; 107 konidia/ml; 108 konidia/ml. Pengamatan dilakukan setiap hari selama 10 hari setelah aplikasi (hsa) dengan menghitung 10 imago C. formicarius yang mati akibat disemprot dengan suspensi cendawan L. lecanii sebanyak 1 ml/perlakuan. Hasil penelitian menunjukkan bahwa kerapatan konidia L. lecanii 108 konida/ml memberikan hasil paling efektif terhadap mortalitas C. formicarius sebesar 50% dengan nilai LT50 sebesar 10,024 hari. Dengan demikian, cendawan entomopatogen L. lecanii sebagai agens hayati C. formicarius layak untuk dikembangkan lebih lanjut.
Respon Pertumbuhan dan Karakteristik Miselia F3 Isolat FP007 Jamur Merang (Volvariella volvaceae) FAPERTA UNSIKA Pada Media yang Berbeda secara In Vitro. Apriliahetty, Laila Ais; Afifah, Lutfi; Samaullah, Mohammad Yamin; Lestari, Ani
JURNAL AGROPLASMA Vol 10, No 2 (2023): JURNAL AGROPLASMA VOLUME 10 NO 2 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i2.4381

Abstract

Straw mushroom (Volvariella volvaceae) is a horticultural product and one of the many tropical and subtropical mushroom species known and in demand by the people of Indonesia. Currently the growth of straw mushroom is still low, this is because the quality seeds are not sufficient and the price is relatively expensive. Quality seeds are not only determined from superior mushroom broodstock, but also determined by the quality of the pure culture, the production of pure culture is constrained by the type of material for making in vitro media. The purpose of this study was to obtain the type of in vitro media that gave the highest growth response and features of the F3 mycelial isolate FP007 mushroom Faperta Unsika. The research method used was by using a single factor Complete Randomized Design (CRD), there were 5 replications and 7 treatments consisting of: 100% PDA (A), 100% Charcoal Husk (B), 100% Husk (C), 80 PDA % + Husk 20% (D), PDA 80% + Charcoal Husk 20% (E), PDA 60% + Husk 20% + Charcoal Husk 20% (F), and Husk 50% + Charcoal Husk 50%. The results of this study showed that 80% PDA media + 20% rice husk charcoal had the highest effect on the growth response and characteristics of the F3 mycelia isolate FP007 straw mushroom Faperta Unsika with a mushroom mycelia growth diameter of 7 cm. Rice husk charcoal media can be used as a good additive for the manufacture of growing media in vitro. Keywords : Charcoal husk, rice husk, pure culture, straw mushroom.
Respons Karakter Agronomi pada Tanaman Jagung (Zea mays) Varietas Pioneer 36 Akibat Aplikasi berbagai Teknik Pengendalian Organisme Pengganggu Tumbuhan Ardian, Fahmi; Afifah, Lutfi; Sugiarto, Sugiarto; Prabowo, Dwi Priyo; Irfan, Budi; Widiawan, Aditia Bagus
JURNAL AGROPLASMA Vol 9, No 2 (2022): AGROPLASMA VOL 9 NO 2
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v9i2.3165

Abstract

The application of plant pest management techniques can affect agronomic character of crops, because there are active ingredients that acts as biofertilizer and biostimulant. This study aims to obtain the best agronomic character as a result the application of various active ingredientson corn crops (Zea mays) of the variety of Pioneer 36. The research method used was an experimental method, with a single-factor group randomize design, consisting of 4 treatments and 6 replications. The treatments of plant pest management consists of control (C), combination management (CM) a.i. PGPR, macro nutrient and micro nutrient; biointensive management (BM) a.i. Trichoderma hamatum and rice husk ash; synthetic management (SM) a.i. pyraclostrobin. The data obtained were analyzed variance and if the results were significantly different, it was followed by the least significant difference (LSD) at the level of 5%. The results showed that, the CM treatments gave the highest results on plant height (10,66 cm-248,78 cm) and the number of rows per cob (14,18). Meanwhile, the SM treatments gave the highest results on the length of cob without corn husk (17,44 cm) and the diameter of cob without corn husk (4,13 cm).Key words: PGPR, Trichoderma hamatum, rice husk ash, pyraclostrobin.
Uji Efektivitas Aplikasi Cendawan Entomopatogen Metarhizium anisopliae Terhadap Mortalitas Wereng Batang Coklat (Nilaparvata lugens Stal.) Prisilya, Erika; Afifah, Lutfi; Sugiarto, Sugiarto; Kurniati, Anik
JURNAL AGROPLASMA Vol 10, No 2 (2023): JURNAL AGROPLASMA VOLUME 10 NO 2 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i2.4503

Abstract

Nilaparvata lugens is one of the harmful pests that causes the quantity and quality of rice yields to decrease. The use of chemical pesticides to control pests has a negative impact on the environment and farmers. Biological control using the entomopathogenic fungus Metarhizium anisopliae safe to use to treat pest attacks. The purpose of this study was to determine the effectiveness of using the entomopathogenic fungus M. anisopliae on mortality N. lugens. The method used in this study is an experimental method with a single factor Completely Randomized Design (CRD). This study used liquid suspension conidia density of M. anisopliae consisting of 5 treatments and 4 repetitions: A (sterile distilled water) as control; B (density 106 conidia/ml); C (density 107 conidia/ml); D (density 108 conidia/ml); E (density 109 conidia/ml). Each experimental unit was infested with 10 instar 3 N. lugens and observed mortality for 7 days after application (hsa). The highest mortality calculation result for N. lugens was shown by application of M. anisopliae suspension at density 10 9 conidia/ml of 97,50% and probit analysis yielded an LC50 value of 8,78 x 108 conidia/ml which killed 50% N. lugens pests. Biological control using the entomopathogenic fungus M. anisopliae is effective for controlling N.lugens and high conidia density can infect the pest with higher mortality.
Uji Ketahanan Beberapa Calon Varietas Jagung Manis (Zea mays L. saccharata Sturt.) Hibrida F1 UNSIKA Terhadap Penyakit Penting Jagung di Purwakarta Dimaspatti, Satrio; Syafi’i, Muhammad; Afifah, Lutfi; Rakhman, Fathu
JURNAL AGROPLASMA Vol 10, No 1 (2023): JURNAL AGROPLASMA VOLUME 10 NO 1 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i1.3658

Abstract

One of the causes of the decreased production of sweet corn (Zea mays L. saccharata Sturt.) is the attack  by disease. Some important diseases that attack sweet corn is leaf blight and leaf rust. Use of hybrid varieties one of the solution which can be used to reduce the crop loss caused by disease. This study aimed to testing the level of resistance of sweet corn hybrid candidates against important corn disease. The research was conducted in the Ambu Garden, Pusakamulya Village, Kiarapedes sub District, Purwakarta District, from July 2021 to September 2021. The design used was a non-factorial randomized block design (RAK) with 33 genotypes of prospective hybrids and 3 commercial comparison varieties and 3 replications. The test result on leaf blight and leaf rust there are several hybrids candidate shows a resistant to moderately resistant reaction. From testing against leaf blight, there are 8 hybrids candidate showed a resistant reaction to moderately resistant. The test result against leaf rust disease, there are 9 hybrids candidate showed resistant to moderately resistant reaction. Overall, the test results against leaf blight disease and leaf rust disease there are  six candidates of sweet corn hybrids that show resistant reaction against both disease, there is UNSIKA 33, 35, 36, 37, 41, and 44 with an average attack intensity of 20,00%. Keywords: Hybrid varieties,Leaf blight, Leaf rust, Sweet corn
Kelimpahan Serangga Pada Berbagai Perangkap Dengan Beberapa Teknik Pengendalian Berbeda Pada Pertanaman Jagung Pioneer 36 Iswara, Dana; Afifah, Lutfi; Abadi, Slamet; Prabowo, Dwi Priyo; Irfan, Budi; Widiawan, Aditya Bagus
JURNAL AGROPLASMA Vol 9, No 2 (2022): AGROPLASMA VOL 9 NO 2
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v9i2.3173

Abstract

Serangga hama adalah hewan yang dapat menyebabkan kerugian pada petani. Pengendalian serangga hama dapat menggunakan aplikasi pestisida ataupun menggunakan perangkap. Tujuan dari penelitian ini adalah untuk mengetahui kelimpahan serangga pada masing masing perangkap terhadap beberapa teknik pengendalian berbeda. Metode eksperimental yang digunakan yaitu dengan menggunakan Rancangan Acak Kelompok (RAK) faktor tunggal, terdapat 6 ulangan dan 4 perlakuan yang terdiri dari : Pengendalian  Sintetik (PS),Pengendalian Kombinasi (PK), Pengendalian Biointensif (PB), dan Kontrol (K). Pengambilan sampel serangga dilakukan sebanyak 13 kali pada 1-13 mst dengan menggunakan 3 jenis perangkap yaitu lubang jebakan (pitfall trap), perangkap kuning lengket (yellow sticky trap) dan jaring serangga (sweep net). Data yang didapatkan dianalisis dengan sidik ragam, dan apabila hasilnya berbeda nyata dilanjut dengan uji Beda Nyata Terkecil (BNT) pada taraf 5 %. Hasil penelitian menunjukan kelimpahan tertinggi terdapat pada petak kontrol (26.935 individu) dan terendah pada pengendalian  sintetik (15.788 Individu). Pengendalian sintetik berbeda nyata dengan pengendalian biointensif, pengendalian kombinasi dan kontrol pada rerata kelimpahan keseluruhan,  yellow sticky trap dan pada rerata kelimpahan pitfall trap pengendalian biointensif tidak berbeda nyata dengan perlakuan pengendalian sintetik tetapi berbeda nyata dengan pengendalian kombinasi dan kontrol. Pada sweep net mengahasilkan tidak berbeda nyata untuk semua pengendalian akan tetapi pengendalian sintetik memberikan rerata kelimpahan terendah yaitu 481.33 individu. Pada yellow sticky trap memberikan kelimpahan tertinggi yaitu 69.239 individu dibandingkan pitfall trap (9.835 individu) dan sweep net (14.347 individu). Hal ini menunjukan penggunaan pestisida sintetik mampu menurunkan kelimpahan serangga. Kata Kunci : Kelimpahan, Pengendalian, Perangkap, Serangga
Co-Authors Abadi, Slamet Abubakar Abubakar Adelita, Shafa Adhi, Satriyo R. Afifah, Dhiya Mutsla Alfarizi, Muhammad Zuhal Alwi, Fauzan Amelia, Nada Ani Lestari Apriliahetty, Laila Ais Ardian, Fahmi Aulia, Gebby Reza Bahruzin Bahruzin, Bahruzin Bhekti, Lintang Cahya Budi Irfan Dimaspatti, Satrio Dodin Koswanudin Enri, Ultach Hasanah, Siti Nissaul Hidayah, Vania Utami Ihsan, Alya Khairani Irfan, Budi Iswara, Dana Khoerunnisa, Khoerunnisa Khoirunnisya, Hylda Aulia Kholifatunnisa, Fani Sri Kulsum, Siti Yasmin Fauzia Kurniati, Anik Latifah, Lia Maryana, Rosalia Mubarokah, Annafs Zakiyyah Mustikasari, Fauzia Mutmainah, Fionni Sholihah Nopiani, Siti Nurcahyo W. Saputro Prabowo, Dwi Priyo Pramudyawardani, Estria Furry Pratiwi, Nabila Dian Prisilya, Erika Putri Kemala Dewi Rahmadianti, Shifani Shintya Rakhman, Fathu Ratna Sari Dewi Roza, Celvia Samaullah, Mohammad Yamin Sandi Ramadhan Saputro, Nurcahyo W. SATMOKO ADI, AGUS Satriyo Restu Adhi Senja, Safira Nurmala Subagyo, Vani Nur Oktaviany Sugiarto Sugiarto S Sugiono, Darso Surjana, Tatang Syafi’i, Muhammad Syafi’i, Muhammad Taufiqullathif, Muhammad Hammad Ultach Enri Widiawan, Aditia Bagus Widiawan, Aditya Bagus Yuliani, Desi Yustiano, Anton