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All Journal Jurnal Ilmu Dasar JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION)
Maryana, Rosalia
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Agriculture, Biological Sciences & Forestry Control & Systems Engineering Environmental Science Mathematics

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Production of The Entomopathogenic Fungi Beauveria bassiana using Starch Rich Substrate and Its Infectivity against Tribolium castaneum Afifah, Lutfi; Afifah, Dhiya Mutsla; Surjana, Tatang; Kurniati, Anik; Maryana, Rosalia
Jurnal ILMU DASAR Vol 23 No 2 (2022)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v23i2.21435

Abstract

The entomopathogenic fungi of Beauveria bassiana is known to infect the Tribolium castaneum pest. Mass production of B. bassiana is currently carried out using alternative media, the amount of conidia produced on alternative media is quite large and can tolerate temperature. This research aims to select alternative media for the propagation of B. bassiana to gain germination, conidia density, and virulence of the best alternative media concentration against the pest of T. castaneum. The method used was a compiled in the Completely Randomized Design (RAL) at all stages of research. In the first stage of alternative media selection consisting of 4 treatments in 6 replications: A (PDA); B (peanuts); C (soybean); D (old coconut meat). The second stage of alternative media infectivity consisting of 5 treatments in 5 replications: positive control (synthesis insecticide); negative control (aquades); 107; 108; 109 spores/ml. The results achieved that alternative media selection given a highest colony diameter is 4.39 cm on soybean, the highest conidia density is 5,61 x 108 spores/ml on peanuts, the highest germinating power is 31.76% on peanuts. Furthermore, the infectivity of alternative media obtained on peanut media had a significant effect on the mortality of T. castaneum (36.00%) at a concentration of 109 spores/ml, LC50 was obtained at 5.6 x 105 spores/ml, and LT50 within 5.42 days. The results indicated that the peanut media was a good propagation medium of B. bassiana and it was necessary to find other alternatives to increase the infectivity of B. bassiana against T. castaneum.
Identifikasi dan Penentuan Pohon Filogenetik Spodoptera frugiperda Asal Jawa Berdasarkan Analisis Sekuen MtDNA COI Widarti, Ani; Tauruslina, Enie; Faridah, Idah; Bagariang, Willing; Suyanto, Hadi; Mahmudah, Didah; Susanti, Rista; Maryana, Rosalia; Carwika, Carwika
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 6 No. 1 (2022): June 2022
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.6.1.43-52.2022

Abstract

Molecular confirmation of the existence of Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) on Java Island has not been widely reported. This study aimed to identify the armyworm from Java Island molecularly based on DNA mitochondrial cytochrome oxidase I (MtDNA COI). There were six armyworms collected from West Java Province (Cirebon - JWB1, Garut - JWB2), Central Java Province (Wonogiri - JWTH), East Java Province (Gresik - JWTR), DI Yogyakarta Province (Bantul - DIY), and Banten Province (Serang - BNT). The results showed that the fall armyworm originating from Java was verified as Spodoptera frugiperda. There were species variations between them, which had species similarity between regions and countries, namely the species similarity of S. frugiperda Java and West Sumatra, as well as to China, Korea, and India. S. frugiperda from Central Java (JWTH), Banten (BNT), West Java 1 (JWB1), and Yogyakarta (DIY) had species similarity to S. frugiperda from Solok (No. Acc. MW876212.1), West Pasaman (No. Acc. MW876211.1), China (No.Acc. MK591010.1) and Korea (No.Acc. MN427930.1). Meanwhile, S. frugiperda West Java (JWB2) and East Java had species similarity to S. frugiperda from India (No. Acc. MT732476.1), Padang Pariaman (No. Acc. MW876208.1), and Tanah Datar (No. Acc. MW876210.1).
Co-Authors Afifah, Dhiya Mutsla Bagariang, Willing Carwika, Carwika Faridah, Idah Kurniati, Anik Lutfi Afifah, Lutfi Mahmudah, Didah Surjana, Tatang Susanti, Rista Suyanto, Hadi Tauruslina, Enie Widarti, Ani