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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Jurnal Ilmu Kimia Universitas Brawijaya The Journal of Pure and Applied Chemistry Research Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Jurnal Surya Masyarakat Journal Syifa Sciences and Clinical Research (JSSCR) Fullerene Journal of Chemistry Jurnal Gema Ngabdi ACADEMICS IN ACTION Journal of Community Empowerment Abdimas UNWAHAS : Jurnal Pengabdian Masyarakat UNWAHAS Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) Jurnal Surya Masyarakat PROFESSIONAL HEALTH JOURNAL JIFI (Jurnal Ilmiah Farmasi Imelda) Indonesian Journal of Community Dedication in Health (IJCDH)
Janatun Na’imah
Program Studi D3 Farmasi, Fakultas Kesehatan, Universitas Muhammadiyah Gresik, Gresik, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : The Journal of Pure and Applied Chemistry Research

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Studies of In Vitro and In Silico of Immobilized Xylanase on Zeolite Matrix Activated with Hydrochloric Acid Janatun Na’imah; Sasangka Prasetyawan; Arie Srihardyastutie
The Journal of Pure and Applied Chemistry Research Vol 6, No 3 (2017): Edition of September - December 2017
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.483 KB) | DOI: 10.21776/ub.jpacr.2017.006.03.335

Abstract

Xylanase is a hydrolase enzyme that can hydrolyze hemicellulose into xilo-oligosaccharide and xylose. This research is aimed to investigate the in vitro and in silico xylanase (isolated from the fungus Trichoderma viride) immobilized on the zeolite matrix activated with HCl 0.4 M solution. The study was performed using in silico docking molecular methods to investigate the interaction between the xylanase primarily to its ligand. Xylanase activity was determined by reducing sugar produced (xylose) by 1 mL of enzyme per minute. The optimum conditions of immobilized xylanase were measured according to the time agitation and concentration of xylanase. The time variation agitation used were 1, 2, 3, 4, and 5 hours, while variations in the concentrations of a xylanase used were 11.500, 15.653, 20.444, 25.875, and 31.944 mg/mL. The optimum conditions of immobilized xylanase was obtained in the shaking time for 3 hours at a concentration of xylanase 15.653 mg/mL and immobilized xylanase activity generated at 46.755 μg.g-1.min-1. Immobilized xylanase activity was greater than the purified xylanase (15.976 μg.mL-1.min-1). These results were due to the cofactors Al (AlO4) and Si (SiO4) of zeolite was able to increase the kinetic energy caused the reaction rate between xylanase with the larger substrates. Cofactor also increased the kinetic energy and can enhance the rate of reaction between a xylanase with its substrate, in order to give greater activity. Immobilized xylanase was stable and its reusability as much as 6 times which afforded the activity 21.331 μg.g-1.min-1 and the efficiency of 56.77%.
Co-Authors Anna Roosdiana Arie Srihardyastutie Chanif Mahdi Diah Ratnasari Diah Ratnasari Dini Kesuma Faidah, Anis Heru Baskoro indahsari, dita dwi Nasyanka, Anindi Lupita Norainny Yunitasari Nur Dya Puspitasari Pemta Tiadeka Riskha Aulia Sasangka Prasetyawan shofiyah, lailatus Sholikha, Rizkia Ayu Siti Asiyah Siti Nur Asiyah Siti Nur Asiyah