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All Journal VALENSI Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan JURNAL KIMIA SAINS DAN APLIKASI Alchemy : Journal of Chemistry Biology, Medicine, & Natural Product Chemistry EduChemia: Jurnal Kimia dan Pendidikan Indonesian Journal of Chemical Science International Journal Of Science, Technology & Management (IJSTM) Jurnal Pengabdian Vokasi
Damar Nurwahyu Bima
Chemistry Department, Faculty Of Sciences And Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang|Diponegoro University|Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Transportation

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Journal : Alchemy : Journal of Chemistry

 1 
Penentuan Aktivitas Antioksidan Senyawa Kuersetin dan Ekstrak Lengkuas Menggunakan HPLC dan UV-Vis Bambang Cahyono; Christiana Suci Prihatini; Meiny Suzery; Damar Nurwahyu Bima
ALCHEMY:Journal of Chemistry Vol 8, No 2 (2020): ALCHEMY: Journal of Chemistry
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v8i2.10594

Abstract

The radical scavenging activity of 1,1-diphenyl-2-picrylhidrazyl (DPPH) is generally carried out using a spectrophotometric method. In this study, the value of the antioxidant activity was compared to the HPLC method. Samples used were pure quercetin and extracts of red galangal (Alpinia purpurata) and white galangal (Alpinia galanga). Analysis of antioxidant activity using a UV/Vis spectrophotometer was carried out at a wavelength of 515 nm. Analysis by HPLC method was carried out using an inverse phase with a UV/Vis detector at 515 nm. The results showed that the radical scavenging activity (IC50) of the pure quercetin produced was nearly the same value for the spectrophotometric method (16.24 ppm) dan the HPLC method (15.24 ppm). Even though, the antioxidant activity of pure quercetin was different from the extract. The red galangal extract gave IC50 of 488.43±1.13 ppm (spectrophotometric method) and IC50 of 68.12±10.19 ppm (HPLC method). The radical scavenging activity (IC50) of white galangal extract using the spectrophotometric method and HPLC method was 462.47±2.98 and 62.17±3.87 ppm, respectively. The allegation of other molecular interference in the radical reduction of the extract resulted in a conclusion that the HPLC is better than the spectrophotometric method for determining antoxidant activity of extract sample. Keywords: Antioxidant, Alpinia purpurata, Alpinia galanga, DPPH  Aktivitas peredaman radikal 1,1-diphenyl-2-picrylhidrazyl (DPPH) pada umumnya dilakukan menggunakan metode spektrofotometer. Dalam penelitian ini, nilai aktivitas antioksidan tersebut telah dibandingkan dengan metode HPLC. Sampel ujinya adalah senyawa kursetin murni dan ekstrak lengkuas merah (Alpinia purpurata) dan lengkuas putih (Alpinia galanga). Analisis aktivitas antioksidan dengan spektrofotometer UV/Vis dilakukan pada panjang gelombang 515 nm. Analisis dengan metode HPLC dilakukan menggunakan fasa inverse dengan detektor UV/Vis pada 515 nm. Hasil penelitian menunjukkan bahwa aktivitas peredaman radikal senyawa murni kuersetin menghasilkan nilai yang hampir sama untuk kedua metode yaitu IC50 sebesar 17,05 ppm untuk metode spektrofotometer dan IC50 sebesar 15,74 ppm untuk metode HPLC. Akan tetapi, nilai aktivitas antioksidan kuersetin jauh berbeda untuk ekstrak. Ekstrak lengkuas merah memberikan IC50 sebesar 488,43±1,13 ppm (metode spektrofotometer) dan 68,12±10,19 ppm (metode HPLC). Aktivitas peredaman radikal ekstrak lengkuas putih dengan metode spektrofotometer dan HPLC dengan IC50 sebesar 462,89±5,38 dan 62,17±3,87 ppm, berturut-turut. Dugaan adanya interferensi molekul lain dalam analisis peredaman radikal terhadap ekstrak ini menghasilkan suatu kesimpulan bahwa metode HPLC lebih baik digunakan dalam analisis antioksidan dibandingkan metode spektrofotometer untuk sampel berupa ekstrak. Kata kunci: Antioksidan, Alpinia purpurata, Alpinia galanga, DPPH
Optimization of Electrocoagulation Conditions in the Degradation Process of Carbofuran Waste (Furadan 3GR) Using Al/Graphite Electrodes Damar Nurwahyu Bima; Kumala Devi; Suhartana Suhartana; Sriyanti Sriyanti
ALCHEMY:Journal of Chemistry Vol 11, No 1 (2023): ALCHEMY: JOURNAL OF CHEMISTRY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v11i1.15360

Abstract

Electrocoagulation is one of the electrochemical methods for treating wastewater and separating pollutants. In this research, the electrocoagulation using Al/Graphite electrodes was applied to artistic wastewater containing carbofuran to see the reduction of its chemical oxygen demand(COD). The electrocoagulation process was chosen in this study based on several advantages, including easy to separate between impurities and the sample and a simple operating system. Several parameters such as electrolysis time, variations in electrolyte concentration, and the effect of pH have been investigated for their impact on the electrocoagulation process. The results showed that the proportion of the best reduction in chemical oxygen demand (COD) was 64.40%. The best conditions were an electrolysis time of 60 minutes, an electrolyte concentration of 0.15 M, and a pH of 10. The results also show that the decrease in chemical oxygen demand (COD) dependson those parameters. These results were investigated further by characterization using UV-Vis spectrophotometry and FTIR spectroscopy. The results of the UV-Vis spectrum showed a decrease in intensity at a wavelength of 275 nm. Analysis of the FTIR spectrum between the initial sample and the results of the electrocoagulation showed that there were similar absorption peaks but with much smaller intensity, indicating that the effluent's pollutants have been deposited.Keywords: Electrocoagulation, Carbofuran, Al/Graphite, COD
Co-Authors Amalina, Nur Dina Ayu Sri Wahyuni Bambang Cahyono Choiril Azmiyawati Christiana Suci Prihatini Deana Wahyuningrum Herayati, Herayati Indah Puspita Sari, Indah Puspita Irma Mulyani Ismiyarto Ismitarto Khairini Pertiwi Kumala Devi Liswinda Zafirah Rahmatia Meiny Suzery Mohd Ridwan Mukhammad Asy'ari Nafisa Khairunisa Ngadiwiyana M.Si. S.Si. Niken Windi Saputri Nor Aida Zubir Nor Basid Adiwibawa Prasetya Nudin, Burhan Purbowatiningrum Ria Sarjono Seno Darmanto Sriatun Sriatun Sriyanti Sriyanti Suhartana Suhartana Yulyani Nur Azizah Yuniarto Yuniarto, Yuniarto Yusuf Umardani