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All Journal Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Indonesian Journal of Cancer Chemoprevention Mutiara Medika: Jurnal Kedokteran dan Kesehatan Jurnal Ilmu Kefarmasian Indonesia Jurnal Kedokteran Meditek
Puspita Eka Wuyung
Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Peranan Gen HOXA10 terhadap Infertilitas Terkait Endometriosis Salinah Salinah; Ria Kodariah; Puspita Eka Wuyung
JURNAL KEDOKTERAN DAN KESEHATAN Vol 7, No 1 (2020)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (572.459 KB) | DOI: 10.32539/JKK.V7I1.8294

Abstract

Endometriosis merupakan penyakit yang bersifat progresif, kronik, rekuren dan hingga saat ini etiologi pastinya belum diketahui. Gejala yang ditimbulkan endometriosis bervariasi mulai dari asimptomatik, nyeri pelvis hingga infertilitas. Beberapa studi terakhir di bidang biologi molekular menunjukkan adanya kemungkinan endometriosis sebagai penyakit epigenetik dan salah satu gen yang diketahui mengalami perubahan epigenetik adalah gen HOXA10. Gen HOXA10 berperan mengatur reseptivitas endometrium selama proses implantasi. Keberhasilan proses implantasi merupakan salah satu faktor yang berperan pada fertilitas wanita dan Gen HOXA10 memiliki peranan penting dalam proses implantasi tersebut. Metilasi gen HOXA10 mengalami peningkatan yang signifikan pada endometrium eutopik wanita yang mengalami endometriosis. Pada endometrium wanita yang mengalami endometriosis terdapat penurunan ekspresi HOXA10 yang signifikan dan hal tersebut mengindikasikan adanya gangguan pada reseptivitas uterus yang dapat berperan dalam menurunnya tingkat kesuburan.  
Differences in MMP-2 Expression in High- and Low-Grade MPNST and its Correlation with Prognostic Factors Niniek Hardini; Nurjati Chairani Siregar; Puspita Eka Wuyung
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 2 (2021): July
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i2.10882

Abstract

Malignant peripheral nerve sheath tumor (MPNST) is a soft tissue sarcoma, which is difficult to distinguish from other spindle cell sarcomas. MPNST is hostile, with a high recurrence, and tends to metastasize hematogenously, especially to the lungs. A phase of the metastasis is a degradation of the extracellular matrix, where Matrix Metalloproteinase (MMP) plays an essential role in this process. Gelatinase-type MMP, MMP-2 and MMP-9, can degrade basal membrane and fibrillar collagen to open the invasion pathway. MMP-2 can degrade more collagen and non-collagen extracellular matrix than MMP-9. Therefore, the study aimed to see the relationship between MMP-2 overexpression and histopathological malignancy grading and other clinical prognostic variables. The study was conducted by immunohistochemical staining of MMP-2 in 39 cases, consisting of 19 cases of low-grade MPNST and 20 cases of high-grade MPNST. Subsequently, an analysis of the relationship between MMP-2 overexpression and the malignancy grading and clinical variables was performed, such as age, sex, and tumor size and location. MMP-2 overexpression was seen in 19 (95%) cases of high grade and three (15.8%) cases of low-grade MPNST (p 0.000). The study also found a significant relationship between MMP-2 overexpression and histopathology grading, which may be helpful to define the prognosis.
Hewan Model Kanker Ovarium untuk Studi Preklinik dan Pengembangan Obat Kanker Ovarium Ni Made Dwi Sandhiutami; Puspita Eka Wuyung; Wawaimuli Arozal; Melva Louisa; Deni Rahmat
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.923 KB) | DOI: 10.35814/jifi.v17i2.734

Abstract

Treatment for ovarian carcinoma is still far from optimal, animal models are still needed to study human epithelial ovarian cancer. Animal models of ovarian cancer are very important for understanding the pathogenesis of the disease and for testing new treatment strategies. Ovarian carcinogenesis models in mice have been modified and repaired to produce preneoplastic lesions and neoplastic ovaries that are pathogens resembling human ovarian cancer. Although spontaneous ovarian tumors in mice have been reported, some of the shortcomings of existing studies preclude their use as animal models of ovarian cancer. Because of this, many efforts have been made to develop animal models that are relevant for ovarian cancer. Experimental animal models are developed accurately to represent cellular and molecular changes associated with the initiation and development of human ovarian cancer. Accurate experimental models have significant potential in facilitating the development of better methods for early detection and treatment of ovarian cancer. Several animal models of ovarian cancer have been reported, including manipulation of various reproductive factors or exposure to carcinogens. The latest advance in ovarian cancer modeling is using genetically engineered mice.
The Effectiveness of Topical 5-fluorouracil Treatment on Mouse Skin Squamous Cell Precancerous Lesions through Caspase-3 Expression Siti Nurkasanah; Aida S.D. Hoemardani; Evlina Suzanna Sinuraya; Puspita Eka Wuyung
Indonesian Journal of Cancer Chemoprevention Vol 13, No 1 (2022)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev13iss1pp12-21

Abstract

Skin cancer is a disease that develops in the epidermis of the skin and can be invasive, such as squamous cell carcinoma (SCC). Early detection of squamous cell precancerous can prevent these lesions from progressing to invasive SCC and increase the effectiveness of therapy. 5-fluorouracil (5-FU) is an antimetabolite compound as a pyrimidine DNA/RNA antagonist molecule that induces cell apoptosis. The main objective of this study was to evaluate the effectiveness of the topical 5-FU cream (Dharmais NCH) compared to imiquimod 5% on apoptosis through the expression of caspase-3 in precancerous squamous cells of mouse skin induced by 7,12-dimethylbenzen[a]-anthracene (DMBA)/croton oil treatment. This research assess three differences concentration of 5-FU include 1%, 2%, and 5% on 24 wild type mouse divided into 6 groups including positive control (with carcinogenesis but without treatment), negative control (without treatment; normal), carcinogenesis with treatment 5-FU cream (1%, 2%, and 5%) or 5% imiquimod cream. Two-stages carcinogenesis induced by DMBA and followed by croton oil. The expression of caspase-3 was analyzed using immunohistochemistry. Statistical analysis was performed by one-way ANOVA using SPSS version 23. The induction of two-stages of carcinogenesis (weeks 1 to 10) caused papilloma lesions on the skin of mouse. Furthermore, 5-FU treatment for 4 weeks (weeks 11 to 14) showed a decrease in the cumulative number of papillomas (p<0.05) and immunohistochemical analysis showed caspase-3 expression on 5-FU treatments (1%, 2%, and 5%) was not significantly different from the imiquimod treatment (p>0.05). The apoptotic effect of 5-FU treatment on precancerous skin squamous cell lesions in mouse was not significantly different from the standard treatment using imiquimod. This suggests that 5-FU treatment has potential as a future therapy in squamous cell precancerous skin lesions.Keywords: 5-fluorouracil, caspase-3, squamous cell precancerous, skin, topical treatment.
Peranan miRNA-200 dalam tahapan metastasis kanker Salinah; Puspita Eka Wuyung
Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Vol. 6 No. 1 (2019): Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universi
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/jkk.v6i1.113

Abstract

MikroRNA merupakan bagian dari keluarga besar non-coding RNA yang secara negatif meregulasi ekspresi gen pengkode protein paska transkripsi melalui pasangan basa antara 5’ seed region miRNA dengan 3’ untranslated region dari mRNA. Berbagai studi telah menunjukkan adanya peran penting miRNA pada perkembangan kanker. Keluarga miR-200 termasuk mikroRNA yang berperan dalam inisiasi hingga tahap metastasis kanker. miR-200 terdiri dari 5 anggota yaitu miR-200a, -200b, -200c, -141, dan -429. Keluarga miR-200 berperan pada tiap tahapan metastasis yaitu mulai dari pertumbuhan tumor, angiogenesis, invasi ke jaringan sekitar, transisi dari epitelial ke mesenkimal, migrasi sel tumor, intravasasi sel tumor, kemampuan survival sel tumor di dalam sirkulasi, dan ekstravasasi sel tumor serta kolonisasinya.
Kemiripan Sekuen Mouse Mammary Tumor Virus dengan Human Mammary Tumor Virus pada Kanker Payudara Shintia Christina; Puspita Eka Wuyung
Jurnal Kedokteran Meditek Vol. 17 No. 45 September - Desember 2011
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v17i45.892

Abstract

Abstrackhuman breast cancer incidence varied around the world, where the incidence in Asia is lower than western. Different types if mice in both parts of the world suggestive associated with the incidence of human breast cancer, in which M.domesticus the main carrier of virus more widely available in western. Mouse Mammary Tumor Virus (MMTV) is a type B retrovirus that causes breast cancer in mice. MMTV has a genome consisting of longterminal Repeat (LTR), Pol, Gag, Pro, and Env. MMTV has the ability to tumorigenesis by insertion mutagenesis and may affect genes that are close to the insertion. Transmission of this virus occurs through two channels, namely endogenous and exogenous. Endogenous pathways through the germline, while the exogenous pathway the milk. Several studies have shown the existence of sequence similarity in Env and MMTV LTR by human breast cancer, so that suspected a link between MMTV in human breast cancer. The presence of MMTV in the rate of progression of disease was also found in line, where the higher the degree of malignancy of a tumor also increased the number of MMTV sequences. However, until now we do not know how the MMTV can infect humans, so its role as a cause of human breast cancer is uncertain.Key words : MMTV, breast cancer
Co-Authors Aida S.D. Hoemardani Arleni Bustami Cicilia Febriani Hayuningrum Deni Rahmat Ening Krisnuhoni Evlina Suzanna Sinuraya Heri Wibowo Irwina Eka Deraya Liem, Isabella Kurnia Melva Louisa Muharam Natadisastra Ni Made Dwi Sandhiutami Niniek Hardini Nurjati Chairani Siregar Reni Oktavina Ria Kodariah Ria Kodariah Salinah Salinah Salinah Shintia Christina Siti Nurkasanah Wahyu Pangestuti Lestari Wawaimuli Arozal Zakiyah Zakiyah