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All Journal Khizanah al-Hikmah : Jurnal Ilmu Perpustakaan, Informasi, dan Kearsipan Universa Medicina Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Jurnal Plastik Rekonstruksi Journal of Global Pharma Technology Biomedika
Liem, Isabella Kurnia
Departemen Anatomi Fakultas Kedokteran Universitas Indonesia Indonesia Museum Of Health And Medicine IMERI Fakultas Kedokteran Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Neuroscience Public Health Other

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Cullin 1 is not associated with late-onset preeclampsia Samara, Tjam Diana; Liem, Isabella Kurnia; Prijanti, Ani Retno; Andrijono, Andrijono
Universa Medicina Vol 38, No 1 (2019)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (653.458 KB) | DOI: 10.18051/UnivMed.2019.v38.4-9

Abstract

BackgroundLate-onset preeclampsia (PE) is preeclampsia occurring after 34 weeks of gestational age or later.  Cullin 1 (CUL1), a proangiogenic protein, is expressed in the placenta, where an imbalance between proangiogenic and antiangiogenic proteins during gestation can cause disturbance of trophoblast invasion. This defect results in vascular ischemia that may produce preeclampsia. The objective of this study was to determine the correlation between CUL1 as proangiogenic factor and late-onset preeclampsia. MethodsThis study was of analytical observational cross-sectional design and involved 44 preeclampsia patients with ³34 weeks of gestational age (late-onset PE). The CUL1 level in the subjects’ sera, taken before they gave birth, and in homogenates of their placenta, obtained per vaginam or by cesarean section, were examined by the ELISA technique.  Statistical analysis was performed with the Spearman correlation test with significant p value of <0.05.ResultsMedian maternal age was 31 years and median gestational age was 37 weeks.  Median serum CUL1 was 41.78 pg/mL and median placental homogenate CUL1 was 32.24 pg per milligram of total placental tissue protein. There was no significant correlation between serum CUL1 level and late-onset preeclampsia (r=-0.281; p=0.065). There was also no significant correlation between placental CUL1 level and late-onset preeclampsia (r=-0.166; p=0.281).ConclusionSerum CUL1 and placental CUL1 were not correlated with late-onset preeclampsia. However, this study indicated that low serum CUL1 tends to prolong gestational age in preeclampsia.
Comparison of matrix metalloproteinase-9 and E-cadherin expression in early- and late-onset preeclampsia Samara, Tjam Diana; Wibowo, Heri; Liem, Isabella Kurnia; Prijanti, Ani Retno; Andrijono, Andrijono
Universa Medicina Vol. 40 No. 3 (2021)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2021.v40.200-206

Abstract

BACKGROUNDPreeclampsia (PE) is one of the most common pregnancy complications worldwide. Turnover of villous trophoblast is affected by impaired placental perfusion in preeclampsia. Among the various factors that influence pro and antiangiogenic factors in trophoblast invasion of PE are E-cadherin and matrix metalloproteinase-9 (MMP-9). The current classification scheme differentiates PE into two variants early-onset (EO) and late-onset (LO) PE. The aim of this study was to compare MMP-9 and E-cadherin expression between early- (EO) and late-onset (LO) PE. METHODSThis study used a cross-sectional design involving 26 women with gestational age <34 weeks (EO) and 38 women with gestational age ≥34 weeks (LO) from PE patients. Placentas born to preeclamptic mothers were taken in the form of small pieces of the maternal side to measure the levels of MMP-9 and E-cadherin by the ELISA method. Statistical analysis was assessed using the Mann Whitney and independent t-test with a significant p value <0.05. RESULTSSemiquantitative proteinuria levels were significantly higher in EO-PE group compared to LO-PE group (p=0.000). Mean E-cadherin levels were significant lower in the EO-PE group (125.94 ± 54.22 pg/mg) compared to LO-PE group (157.95 ± 54.12 pg/mg) (p=0.024). However, there was no significance difference in median MMP-9 levels between EO-PE group and LO-PE group (p=0.376). CONCLUSIONThis study demonstrate that E-cadherin had lower levels in preeclampsia patients who gave birth <34 weeks. This study indicated that lower levels of e-cadherin can lead to early delivery in preeclampsia patients.
ANATOMY VISUAL LEARNING: A NEW MODALITY TO ENHANCE NEUROANATOMY LEARNING IN FIRST-YEAR MEDICAL STUDENTS Sasanthy Kusumaningtyas; Mochamad Iskandarsyah Agung Ramadhan; Ria Margiana; Elvan Wiyarta; Reynardi Sutanto; Isabella Kurnia Liem
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 10, No 1 (2021): Maret
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpki.53276

Abstract

Background: In gaining knowledge and comprehension of neuroanatomy, medical students require not only memorizing the anatomical structures but also understanding the spatial relationships. Along with the cadaver prosection usage, we proposed an anatomy visualization learning (AVL) as possible modality to fulfill this need, yet this has to be proven. This study aimed to know whether AVL can improve student knowledge in neuroanatomy and give a positive perception to the student. Methods: A quantitative and qualitative studies were performed to the first-year medical students (n=46) of Faculty of Medicine Universitas Indonesia who were randomly divided into two groups, an interventional (n=23) and a control (n=23) group. Both groups enrolled in the neuroanatomy learning module, with additional two sessions (two hours/session) of AVL using 3D anatomy visualization table for the intervention group. Quantitative study to know the knowledge improvement was done by comparing the pre-test, post-test and gain scores between groups. Student’s perception of the learning method was done using a questionnaire on a Likert scale. Qualitative study was done using open questions. Results: The median value of the pre-test score (8 [0-30] vs. 4 [0-42]; p=0.869) and the post-test scores (44 [30-82] vs. 40 [8-84]; p=0.068) between the interventional vs control group were not different. Nevertheless, the interventional group gained higher scores than the control group (41.6±18.06 vs. 29.8±20.47; CI 95%; p<0.05). Participants (the interventional group) had a positive perception about the AVL usage for neuroanatomy learning. Conclusion: AVL can be considered as a new approach modality for neuroanatomy learning.
Prospect of umbilical cord mesenchymal stem cell culture waste in regenerative medicine Jeanne Adiwinata Pawitan; Melisa Leviana; Dewi Sukmawati; Isabella Kurnia Liem; Ria Margiana; Twidy Tarcisia
Journal of Global Pharma Technology Volume 9 Issue 07
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstract: Objective:to analyzevarious growth factor (GF) content in umbilical cord derived mesenchymal stem cell (UC-MSC) culture waste at harvest.Methods: We cultured UC- MSCs in complete medium, and collected the medium waste at harvests.  We noted the time of harvests, and measured the concentrations of EGF, VEGF, NGF and Pl GF in the various medium wastes using ELISA method. We grouped GF concentrations according to various culture lengthsat harvest, and calculated themean values and standard deviations of the various GF. We compareddescriptively the GF concentrations at various culture lengths at harvests to conclude which condition yielded the overall highest GF concentration.Results: EGF, VEGF and PlGF concentrations were highest at harvest day-3, whileNGF concentration was the highest at harvest-day-5, respectively. Therefore, overall highest concentration of most GF was at harvest day 3.Conclusion:UC-MSC culture waste at harvest day-3 containedEGF, VEGF, NGF and PlGF, which might be used in regenerative medicine.
Satisfaction Evaluation of Museum Integration with Medical Student Practicum Modules Isabella Kurnia Liem; Dini Fitriyanti; Aldila Amini Nasrul; Marcell Fablius Jonathan
Khizanah al-Hikmah : Jurnal Ilmu Perpustakaan, Informasi, dan Kearsipan Vol 11 No 1 (2023): June
Publisher : Program Studi Ilmu Perpustakaan UIN Alauddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/kah.v11i1.cf3

Abstract

The Indonesia Museum of Health and Medicine (iMuseum), since 2019, has been carrying out the Growth and Development Module Integration Practicum for medical students. This practicum integrates basic medical science (Embryology) and clinical (Children's Health) within the scope of human development, from conception to adulthood. To assess the success of the program, an evaluation of student satisfaction questionnaire data has been carried out on the implementation of the 2020-2022 practicum in a cross-sectional manner. A total of 449 respondent data were successfully processed, consisting of FKUI internal (n=308) and external (n=141) student data. Overall, students have good satisfaction, (3.79; SD 0.36). External students have higher satisfaction (3.87; SD 0.23) compared to internal students (3.78; SD 0.32); independent-t test; t=3.395 (t>1.96); p=0.001 (p<0.05). However, this satisfaction score is not correlated with the value post-test (data for the External group 1; Spearman's test p=0.412 [p>0.05]; rs=0.102). Mark post-test the average reached the number A, namely 86.08 (SD 7.77). It was concluded that the Growth and Development Module Integration Practicum has provided satisfaction to medical students properly.
EFFECT OF PRE-NATAL INTERMITTENT MONOTONOUS AND RHYTHMIC AUDITORY EXPOSURES ON BRAINSTEM NUCLEI PLASTICITY OF ONE DAY-OLD CHICK Dodik Nursanto; Isabella Kurnia Liem; Ahmad AuliaJusuf; Deswaty Furqonita
Biomedika Vol 15, No 2 (2023): Biomedika Agustus 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v15i2.3306

Abstract

ABSTRACT Environmental auditory exposure in a form of both enrichment and stressor environmental can differently modulate the development of auditory system Prenatal chronic noise exposure caused neurogenesis and neuroplasticity disorders in the brain's auditory pathway resulting in neurocognitive impairment. Meanwhile exposure to prenatal music positively modifies morphological and biochemical developments in the brain's auditory pathway that supports neurogenesis and neuroplasticity. This study aimed to determine the plasticity of brainstem nuclei after exposure to music, noise, and a combination of both. This research used an experimental method using 24 female chicks as subjects. Subjects were obtained from eggs after incubation, which were divided into 4 groups: control, music, noise, and combined noise and music. Brain stem nuclear plasticity was measured by total number of nuclei, neuronal nuclear area, and synaptophysin expression as parameters. Prenatal music exposure significantly increased the total number of neurons, neuronal nuclear area, and synaptophysin expression in brain stem nuclei (p < 0.001), whereas combined and noise exposure significantly decreased these three plasticity parameters (p < 0.001). In conclusion, prenatal music exposure potential to increase neuroplasticity of brainstem nuclei for better neurocognitive function ABSTRAK Paparan pendengaran lingkungan dalam bentuk enrichment dan stresor lingkungan dapat memodulasi perkembangan sistem pendengaran secara berbeda. Paparan kebisingan kronis prenatal menyebabkan gangguan neurogenesis dan neuroplastisitas pada jalur pendengaran otak sehingga mengakibatkan gangguan neurokognitif. Sementara itu, paparan musik prenatal secara positif mengubah perkembangan morfologi dan biokimia pada jalur pendengaran otak yang mendukung neurogenesis dan neuroplastisitas. Penelitian ini bertujuan mengetahui plastisitas nucleus batang otak setelah paparan musik, kebisingan, dan kombinasi keduanya. Penelitian ini menggunakan metode eksperimen dengan menggunakan 24 ekor anak ayam betina sebagai subjeknya. Subjek diperoleh dari telur setelah diinkubasi, yang terbagi dalam 4 kelompok: kontrol, musik, kebisingan, dan gabungan kebisingan dan musik. Plastisitas nukleus batang otak diukur dengan parameter jumah total nukleus, luas neuronal nuclear area, dan ekpresi synaptophysin. Paparan musik prenatal meningkatkan jumlah total neuron, luas neuronal nuclear area, dan ekspresi synaptophysin di inti batang otak secara signifikan (p< 0,001), sedangkan paparan bising dan kombinasi secara signifikan menurunkan ketiga parameter plastisitas tersebut (p < 0.001). Kesimpulannya, paparan musik prenatal berpotensi meningkatkan neuroplastisitas inti batang otak untuk fungsi neurokognitif yang lebih baik
Application Of Allo Mesenchymal Stem Cells On Chronic Burn Wound: Case Series Report Wardhana, Aditya; Liem, Isabella Kurnia; Feroniasanti, Lauda; Pudjiti, Dyah Juliana; Mujadid, Fajar; Kispa, Tera; Novialdi, Novialdi
Jurnal Plastik Rekonstruksi Vol. 4 No. 1 (2017): Jurnal Plastik Rekonstruksi
Publisher : Lingkar Studi Bedah Plastik Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (640.754 KB) | DOI: 10.14228/jpr.v4i1.217

Abstract

Background : Severe burns are among the commonly occurring trauma with lethal outcome. One of the important aspects of severe burn therapy is to quickly achieve wound healing. Previous reports indicated that mesenchymal stem cells (MSCs) therapy contributes in facilitating better wound healing. In this report, we investigated the effects of MSCs derived from human bone marrow and umbilical cord on wound healing in patients with severe burns and its mechanism. Method : We performed human bone marrow and human umbilical cord MSCs therapy on 3 severe burns patients. Two of the patients had inadequate donor to close raw surface with skin graft, whilst one patient had infected chronic burn wound which have failed to epithelialize despite repeated attempts of skin graft and wound care. Result : We observed that MSCs therapy significantly accelerated wound healing. The effects after MSCs migrated into wound were decreased infiltration of inflammatory cells and faster epithelialization. Conclusion : This study suggests that MSCs therapy has positive effects in improving wound healing in severe burns patients. Data provided by this research may serve as theoretical basis for further study of MSCs application in burn wound therapy.
UC-MSCs Secretome Induces Proliferation of CD4+ T Cells, CD8+ T Cells, NK Cells, and Increases sPD-1 Levels in Severe COVID-19’s Whole Blood Soleha, Winna; Wibowo, Heri; Abdullah, Murdani; Pradipta, Saraswati; Syari, Lucky Novita; Liem, Isabella Kurnia; Bustami, Arleni; Rozaliyan, Anna
Molecular and Cellular Biomedical Sciences Vol 9, No 1 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i1.538

Abstract

Background: Clinical features of severe coronavirus disease 2019 (COVID-19) predominantly include respiratory symptoms and exacerbated multi-organ complications, especially in patients with comorbidities. Cellular immunity, including lymphocytes, is a critical factor in combating SARS-CoV-2 infection. However, immune dysregulation occurs in severe COVID-19 patients, characterized by cytokine storm and lymphopenia. The effectiveness of mesenchymal stem cell (MSC) therapies for COVID-19 is being assessed. The secretome released by MSC functions similarly to the cells themselves as an immunomodulator, offering potential advantages in terms of safety and cost-effectiveness. This study was conducted to assess the effect of umbilical cord MSC-derived (UC-MSC) secretome treatment on lymphocyte count and soluble programmed cell death-1 (sPD-1) levels in severe COVID-19 patient's whole blood.Materials and methods: Twelve whole blood samples from healthy individuals and severe COVID-19 patients were analyzed for lymphocyte count and functional activation using flow cytometry, along with sPD-1 level measurement in pre-treatment and post-secretome conditions.Results: The lymphocyte count in severe COVID-19 patients was significantly decreased, particularly for T cells and NK cells, indicating lymphopenia. Following secretome treatment, CD4+ T cell counts significantly increased compared to pre-treatment, although this change was not significant in the negative control group. Additionally, there was a minimal reduction in B cell count and an increase in sPD-1 levels. Elevated sPD-1 may alleviate T cell exhaustion by interfering with PD-1 binding to programmed death-ligand 1 (PD-L1).Conclusion: Administration of UC-MSC secretome to the whole blood of severe COVID-19 patients suggested immune improvement, with significant increases in CD4+ T cell counts, enhanced B cell survival, and elevated sPD-1 levels. Keywords: COVID-19, cellular immunity, lymphocytes, secretome, MSC
Co-Authors Aditya Wardhana Ahmad Aulia Jusuf Aldila Amini Nasrul Andrijono Andrijono Ani Retno Prijanti Arleni Bustami Deswaty Furqonita Dewi Sukmawati Dini Fitriyanti Dodik Nursanto Elvan Wiyarta Ening Krisnuhoni Fanny Oktorina Feroniasanti, Lauda Firda Asma'ul Husna Heri Wibowo Irwina Eka Deraya Jeanne Adiwinata Pawitan Kispa, Tera Luh Putu Ratna Sundari Marcell Fablius Jonathan Melisa Leviana Mochamad Iskandarsyah Agung Ramadhan Mujadid, Fajar Murdani Abdullah Novialdi . Pradipta, Saraswati Pudjiti, Dyah Juliana Puspita Eka Wuyung Puspita Eka Wuyung Radietya Alvarabie Reni Oktavina Reynardi Sutanto Ria Kodariah Ria Kodariah Ria Margiana Ria Margiana Rozaliyan, Anna Sasanthy Kusumaningtyas Soleha, Winna Syari, Lucky Novita Tjam Diana Samara Twidy Tarcisia Zakiyah Zakiyah