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All Journal Jurnal Biosains Pascasarjana ILKOM Jurnal Ilmiah Narra J
Yosephi, Valensa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Detection of Pseudomonas aeruginosa pus wound isolate using a polymerase chain reaction targeting 16S rRNA and gyrB genes: A case from Indonesia Jamaluddin, Indra P.; Musa, Susan H.; Ethica, Stalis N.; Ansori, Arif NM.; Yosephi, Valensa; Atmaja, Peter Y.; Murtadlo, Ahmad AA.; Sahadewa, Sukma; Durry, Fara D.; Rebezov, Maksim; Derkho, Marina; Naw, Sin W.; Zainul, Rahadian; Rachmawati, Kadek
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.774

Abstract

Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and gyrB genes in P. aeruginosa using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identified with molecular identification. Sequencing and BLAST analysis were carried out to determine the similarity of gene identity by comparing sample sequence with other isolate sequences on the Gene Bank. The results of molecular identification showed amplification DNA band of around 934 base pairs (bp) for 16S rRNA and 225 bp for gyrB gene. The BLAST program demonstrated that the sample had 99.89% similarity with P. aeruginosa strain XC4 (accession code ON795960.1) for the 16S rRNA gene. Meanwhile, the gyrB gene exhibited 99.10% similarity with the P. aeruginosa strain PSA-1.2 (accession code KP172300.1).
THE ROLE OF PROBIOTICS AS ADJUVANT THERAPY IN INFLAMMATORY CARDIOVASCULAR DISEASES Yosephi, Valensa; Syakdiyah, Noer Halimatus; Atmaja, Peter Yustian; Soegiarto, Gatot
Jurnal Biosains Pascasarjana Vol. 26 No. 2 (2024): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v26i2.2024.152-164

Abstract

Cardiovascular diseases are the leading cause of death worldwide. Probiotics are live microorganisms that can provide health benefits to the host when administered in adequate amounts. The anti-inflammatory effects of probiotics were initially studied in Crohn's disease (CD) and ulcerative colitis (UC). This research aim to provide scientific evidence and explanation on the effects of probiotic administration as adjuvant therapy for inflammatory diseases in cardiovascular health. The study employed a descriptive approach using the systematic literature review method on PubMed, Scopus, SAGE, and Web of Science. A total of 13 articles were included in this study. The probiotics used included Bifidobacterium in one clinical trial, Lactobacillus in eight clinical trials, Enterococcus in one clinical trial, a combination of Bifidobacterium and Lactobacillus in one clinical trial, and a combination of Lactobacillus and Propionibacterium in one clinical trial.
The implementation of forward chaining and certainty factor methods to examine the covid-19 vaccination eligibility Herlina, Herlina; Yosephi, Valensa; Hazriani, Hazriani
ILKOM Jurnal Ilmiah Vol 13, No 3 (2021)
Publisher : Prodi Teknik Informatika FIK Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/ilkom.v13i3.973.307-313

Abstract

Screening for the COVID-19 vaccination is an important and mandatory step to ensure the eligibility status of each candidate for vaccination. The main obstacle experienced was the ineffective screening process (a long process) that resulted in the accumulation of patients. Sometimes it takes one to two hours from registration to receiving the vaccination paper. This situation certainly causes discomfort for both participants and vaccinators. Therefore, the presence of an expert system is needed to overcome these obstacles. The expert system plays a role in simplifying service flow and shortening service time. This article discusses the application of the Forward Chaining method to formulate screening parameters so that they can be used to accurately determine vaccination eligibility. Based on the results of interviews with experts (expert doctors who handle vaccination), 12 rules were obtained from the formulation of 20 historical parameters, which will then serve as a knowledge base for the Covid-19 vaccination screening expert system. The test is carried out by comparing the results of expert screening diagnoses with an expert system using 15 samples of test data. The test results using the Certainty Factor method show that the confidence level is 84% to 99%.
Co-Authors Ansori, Arif NM. Atmaja, Peter Y. Atmaja, Peter Yustian Derkho, Marina Durry, Fara D. Ethica, Stalis N. Gatot Soegiarto Hazriani, Hazriani Herlina Herlina Jamaluddin, Indra P. Kadek Rachmawati Murtadlo, Ahmad AA. Musa, Susan H. Naw, Sin W. Noer Halimatus Syakdiyah Rahadian Zainul Rebezov, Maksim Sukma Sahadewa, Sukma