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All Journal Jurnal Biosains Pascasarjana Narra J
Yosephi, Valensa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Detection of Pseudomonas aeruginosa pus wound isolate using a polymerase chain reaction targeting 16S rRNA and gyrB genes: A case from Indonesia Jamaluddin, Indra P.; Musa, Susan H.; Ethica, Stalis N.; Ansori, Arif NM.; Yosephi, Valensa; Atmaja, Peter Y.; Murtadlo, Ahmad AA.; Sahadewa, Sukma; Durry, Fara D.; Rebezov, Maksim; Derkho, Marina; Naw, Sin W.; Zainul, Rahadian; Rachmawati, Kadek
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.774

Abstract

Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and gyrB genes in P. aeruginosa using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identified with molecular identification. Sequencing and BLAST analysis were carried out to determine the similarity of gene identity by comparing sample sequence with other isolate sequences on the Gene Bank. The results of molecular identification showed amplification DNA band of around 934 base pairs (bp) for 16S rRNA and 225 bp for gyrB gene. The BLAST program demonstrated that the sample had 99.89% similarity with P. aeruginosa strain XC4 (accession code ON795960.1) for the 16S rRNA gene. Meanwhile, the gyrB gene exhibited 99.10% similarity with the P. aeruginosa strain PSA-1.2 (accession code KP172300.1).
THE ROLE OF PROBIOTICS AS ADJUVANT THERAPY IN INFLAMMATORY CARDIOVASCULAR DISEASES Yosephi, Valensa; Syakdiyah, Noer Halimatus; Atmaja, Peter Yustian; Soegiarto, Gatot
Jurnal Biosains Pascasarjana Vol. 26 No. 2 (2024): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v26i2.2024.152-164

Abstract

Cardiovascular diseases are the leading cause of death worldwide. Probiotics are live microorganisms that can provide health benefits to the host when administered in adequate amounts. The anti-inflammatory effects of probiotics were initially studied in Crohn's disease (CD) and ulcerative colitis (UC). This research aim to provide scientific evidence and explanation on the effects of probiotic administration as adjuvant therapy for inflammatory diseases in cardiovascular health. The study employed a descriptive approach using the systematic literature review method on PubMed, Scopus, SAGE, and Web of Science. A total of 13 articles were included in this study. The probiotics used included Bifidobacterium in one clinical trial, Lactobacillus in eight clinical trials, Enterococcus in one clinical trial, a combination of Bifidobacterium and Lactobacillus in one clinical trial, and a combination of Lactobacillus and Propionibacterium in one clinical trial.
Co-Authors Ansori, Arif NM. Atmaja, Peter Y. Atmaja, Peter Yustian Derkho, Marina Durry, Fara D. Ethica, Stalis N. Gatot Soegiarto Jamaluddin, Indra P. Kadek Rachmawati Murtadlo, Ahmad AA. Musa, Susan H. Naw, Sin W. Noer Halimatus Syakdiyah Rahadian Zainul Rebezov, Maksim Sukma Sahadewa, Sukma