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All Journal Narra J Quality : Jurnal Kesehatan
Ethica, Stalis N.
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Detection of Pseudomonas aeruginosa pus wound isolate using a polymerase chain reaction targeting 16S rRNA and gyrB genes: A case from Indonesia Jamaluddin, Indra P.; Musa, Susan H.; Ethica, Stalis N.; Ansori, Arif NM.; Yosephi, Valensa; Atmaja, Peter Y.; Murtadlo, Ahmad AA.; Sahadewa, Sukma; Durry, Fara D.; Rebezov, Maksim; Derkho, Marina; Naw, Sin W.; Zainul, Rahadian; Rachmawati, Kadek
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.774

Abstract

Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and gyrB genes in P. aeruginosa using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identified with molecular identification. Sequencing and BLAST analysis were carried out to determine the similarity of gene identity by comparing sample sequence with other isolate sequences on the Gene Bank. The results of molecular identification showed amplification DNA band of around 934 base pairs (bp) for 16S rRNA and 225 bp for gyrB gene. The BLAST program demonstrated that the sample had 99.89% similarity with P. aeruginosa strain XC4 (accession code ON795960.1) for the 16S rRNA gene. Meanwhile, the gyrB gene exhibited 99.10% similarity with the P. aeruginosa strain PSA-1.2 (accession code KP172300.1).
BIOPROSPECT OF BACTERIAL FIBRINOLYTIC PROTEASE FROM BEKASAM OF LONGTAIL TUNA AS ANTITHROMBOTIC AGENT: LITERATURE REVIEW AND BIBLIOGRAPHY STUDY Trianes, Juwy; Ethica, Stalis N.; Afriansyah, M. Ardi; Darmawati, Sri; Rahmani, Nanik; Zilda, Dewi S.
Quality : Jurnal Kesehatan Vol. 18 No. 2 (2024): Quality : Jurnal Kesehatan
Publisher : Politeknik Kesehatan Kemenkes RI Jakarta I

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36082/qjk.v18i2.2016

Abstract

Introduction:Cardiovascular disease (CVD) is the leading cause of death worldwide, with thrombosis being a significant risk factor. Fibrinolytic proteases have potential as antithrombotic agents and could be developed into CVD drugs. Indonesia's traditional fermented products, such as fish Bekasam, are rich in microorganisms, including fibrinolytic protease-producing bacteria, yet their potential for CVD treatment is underexplored. Fish paste in Indonesia serves as a protein source and a reservoir of bacterial diversity, which could aid in discovering new antithrombotic agents. Objective: This literature review examines research trends over the past decade on fibrinolytic protease-producing bacteria in traditional fermented products, with a focus on Bekasam made from Longtail Tuna. This fermented food is an alternative source for obtaining bacteria with antithrombotic properties. Methods: Data for this review were sourced from Google Scholar, PubMed, and the dimension.ai database, using the keyword "bacterial fibrinolytic protease" from 2015 to 2024. Visualization of global research trends was performed using VOS viewer software. Results: The review found a scarcity of studies on fibrinolytic proteases from Bekasam bacteria. Lactic acid bacteria involved in Bekasam fermentation possess proteolytic enzymes that degrade fish protein into peptides and amino acids, potentially offering antithrombotic properties. This suggests natural protease sources from traditional fermented foods have significant biomedical potential. Research on fibrinolytic protease-producing bacteria from Bekasam in Indonesia is limited and requires further development. Exploring these local fermented products could yield innovative sources for thrombosis treatment
Co-Authors Afriansyah, M. Ardi Ansori, Arif NM. Atmaja, Peter Y. Derkho, Marina Durry, Fara D. Jamaluddin, Indra P. Kadek Rachmawati Murtadlo, Ahmad AA. Musa, Susan H. NANIK RAHMANI Naw, Sin W. Rahadian Zainul Rebezov, Maksim Sri Darmawati Sukma Sahadewa, Sukma Trianes, Juwy Yosephi, Valensa Zilda, Dewi S.