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All Journal Bioma : Berkala Ilmiah Biologi BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Mahardika, Wahyu Aji
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Veterinary

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Isolasi dan Karakterisasi Kapang Filoplan serta Serasah Daun di Lingkungan Laboratorium Biologi Universitas Diponegoro Dengan Metode Contact Plate Mahardika, Wahyu Aji; Dion, Romario; Qoys Naufal, Mochammad Fa’iq; Ramadhany, Warih; Lunggani, Arina Tri
Bioma : Berkala Ilmiah Biologi Vol. 23, No 1, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.1.6-10

Abstract

Fungi can be present on various substrates in the surrounding environment. The presence of these molds takes advantage of the nutrients that are attached to or contained in the substrate. The purpose of this study was to isolate and obtain various types of molds from various substrates. The method used in this study is a contact plate by attaching it to various objects or substrates, namely leaf litter, composter walls, tables, glass, and cabinets. The fungi obtained were 6 isolates, including those from the genus Aspergillus, Penicillium, Curvularia, and Alternaria,
RECENT COLLECTION OF THE POISONOUS MUSHROOM, Clarkeinda trachodes (Berk.) FOR INDONESIA Permana Putra, Ivan; Nurhayat, Oktan Dwi; Mahardika, Wahyu AJi; Sari, Okta Yulia
BIOTROPIA Vol. 31 No. 1 (2024): BIOTROPIA Vol. 31 No. 1 April 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.1.2172

Abstract

Clarkeinda trachodes is an interesting agaric and has been acknowledged as one of the poisonous mushrooms worldwide. This species has mentioned to be found in tropical regions of Asia, including Indonesia. However, no clear record has been found regarding the primary information and herbarium collection of this species in Indonesia. During our regular mushroom foraging at IPB University campus forest Indonesia, the basidiomata of Clarkeinda was encountered. The current study aimed to confirm the taxonomical position of our specimens based on morphological and molecular evidence. Fresh basidomata was observed on the macro- and micromorphological features. The molecular analysis and phylogenetic tree construction were done based on ITS 1/2 sequence. The combination of morphological and molecular evidence confirmed our specimen as C. trachodes. Morphologically, Clarkeinda trachodes BO24637 can be determined by a large basidiocarp of Agaricales, prominent pellicle on disc pileus, notable ring, reddish brown context and stipe when injured, and truncate apex of basidiospores. The BLAST result showed that our specimen has a high similarity to C. trachodes as the top hits. In addition, the phylogenetic tree constructed by ITS sequence committed specimen BO24637 as C. trachodes with 100% BS value. The current study resolves a clear and accessible document of C. trachodes in Indonesia. In addition, we provided the morphological and molecular information as well as herbarium collection of C. trachodes in Indonesia, which can be used for future study of this species.
Co-Authors Arina Tri Lunggani Dion, Romario Ivan Permana Putra Nurhayat, Oktan Dwi Qoys Naufal, Mochammad Fa’iq Ramadhany, Warih Sari, Okta Yulia