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All Journal Bioma : Berkala Ilmiah Biologi Jurnal Biologi Universitas Andalas
Ramadhany, Warih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

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Isolasi dan Karakterisasi Kapang Filoplan serta Serasah Daun di Lingkungan Laboratorium Biologi Universitas Diponegoro Dengan Metode Contact Plate Mahardika, Wahyu Aji; Dion, Romario; Qoys Naufal, Mochammad Fa’iq; Ramadhany, Warih; Lunggani, Arina Tri
Bioma : Berkala Ilmiah Biologi Vol. 23, No 1, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.1.6-10

Abstract

Fungi can be present on various substrates in the surrounding environment. The presence of these molds takes advantage of the nutrients that are attached to or contained in the substrate. The purpose of this study was to isolate and obtain various types of molds from various substrates. The method used in this study is a contact plate by attaching it to various objects or substrates, namely leaf litter, composter walls, tables, glass, and cabinets. The fungi obtained were 6 isolates, including those from the genus Aspergillus, Penicillium, Curvularia, and Alternaria,
Characterization And Screening Of Protease, Amylase, And Cellulase From Phylloplane Fungi Isolates Of Avicennia marina (Forssk.) Vierh. Mahardhika, Wahyu Aji; Ramadhany, Warih; Lunggani, Arina Tri
Jurnal Biologi Universitas Andalas Vol 9 No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.54-59.2021

Abstract

Mangrove (Avicennia marina) is an important mangrove species, because it has many benefits for human life, including in the ecological scope as a source of food and medicine. These plants can also be used as a source of symbiont mold isolates which can be developed as an alternative to produce bioactive compounds, one of which is enzymes. Enzymes are protein compounds that can catalyze all chemical reactions in biological systems. This study aims to determine whether mangrove symbiont fungi (A. marina) can produce amylase, protease and cellulase enzymes. This research was conducted using a method based on enzyme activity, namely amylase activity with lugol iodine staining, protease activity and cellulase activity with congo red staining. on agar media enriched with 1% skim milk, 1% starch and 1% CMC. The results showed that 4 isolates were able to show potential enzymatic activity to be developed as agents for enzyme production.
Co-Authors Arina Tri Lunggani Dion, Romario Mahardhika, Wahyu Aji Mahardika, Wahyu Aji Qoys Naufal, Mochammad Fa’iq