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Isolasi dan Karakterisasi Kapang Filoplan serta Serasah Daun di Lingkungan Laboratorium Biologi Universitas Diponegoro Dengan Metode Contact Plate Mahardika, Wahyu Aji; Dion, Romario; Qoys Naufal, Mochammad Fa’iq; Ramadhany, Warih; Lunggani, Arina Tri
Bioma : Berkala Ilmiah Biologi Vol. 23, No 1, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.1.6-10

Abstract

Fungi can be present on various substrates in the surrounding environment. The presence of these molds takes advantage of the nutrients that are attached to or contained in the substrate. The purpose of this study was to isolate and obtain various types of molds from various substrates. The method used in this study is a contact plate by attaching it to various objects or substrates, namely leaf litter, composter walls, tables, glass, and cabinets. The fungi obtained were 6 isolates, including those from the genus Aspergillus, Penicillium, Curvularia, and Alternaria,
Analisis Cemaran Kapang dan Khamir pada Jamu Serbuk Instan Jahe Merah dan Temulawak Dion, Romario; Purwantisari, Susiana
Berkala Bioteknologi Vol. 3 No. 2 November 2020
Publisher : Berkala Bioteknologi

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Abstract

ABSTRACTInstant herbal medicine is a powder or granule form of herbal medicine that is usually made from sugar and spices which are combined with or without the addition of other food ingredients and food additives. Powdered herbal medicine has the advantage that it is practical and fast in serving, and has a relatively long shelf life. However, poor processing of herbal powder can lead to microbial contamination such as mold and yeast. This study aims to determine the presence of mold and yeast contamination contained in the red ginger and ginger powder herbs. The steps taken in this study were sampling, preparation of tools and materials, homogenization and sample dilution, and testing for mold and yeast levels. This research was conducted for 2 months from August to September 2020 at the Biotechnology Laboratory, Department of Biology, Faculty of Science and Mathematics, Diponegoro University. The results showed that the mold and yeast levels found in the red ginger powder herbal sample were 1 x 101 colonies / g, while the mold and yeast levels found in the ginger powder herbal medicine were 21 x 101 colonies / g. of the two samples have met the standards of BPOM RI Regulation Number 32 of 2019 so that it can be concluded that the red ginger and ginger powder herbs are still safe for consumption.Keyword :  Herbal powder, Contaminants, Molds and Yeasts
In silico Exploration of Phospholipase A2 Inhibitor Compounds from Lufariella variabilis as Antivenom of Ophiophagus hannah Dion, Romario; Ewaldo, Muhammad Farrel; Fauzaan, Muhammad Faishal; Wandi, Ilham Aris; Asih, Rina Sari
Biotropika: Journal of Tropical Biology Vol. 10 No. 1 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.01.06

Abstract

The King Cobra (Ophiophagus hannah) is a venomous snake found in Southeast Asia and South Asia. Globally, it is estimated that there are 81,000 to 138,000 cases of snakebite deaths from 1.8 million to 2 million snakebite cases. The limited availability of antivenom is a problem in handling snake venom poisoning. Exploration of natural ingredients is needed as a preventive measurement from the spread of toxins when they are inside the body. Exploration could be carried out by utilizing natural metabolite compounds that can be inhibitors of the phospholipase A2 (PLA2) enzyme. Luffariella variabilis is known as a marine organism that can produce sesterterpenoid compounds and has the potential as an inhibitor of the phospholipase A2 enzyme. This study aims to explore the potential of sesterterpenoid compounds produced by Luffariella variabilis as an in silico inhibitor of phospholipase A2. Several methods used in this research are molecular docking simulation, toxicity test using pkCSM and Toxtree, and chemical bond analysis using Discovery Studio. The results showed that the manoalide compound was the most potent compound of the other sesterterpenoid compounds in its ability to become a snake antivenom candidate.