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All Journal Pharmaciana: Jurnal Kefarmasian Jurnal Kesehatan Anakes: Jurnal Ilmiah Analis Kesehatan Kolaborasi Jurnal Pengabdian Masyarakat Jurnal Pengabdian kepada Masyarakat Biomedika
Muflihah, Ahdiah Imroatul
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Religion Humanities Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Gambaran Morfologi Sel Neutrofil Pada Pewarnaan Giemsa dengan Variasi Waktu Pada Larutan Pengencer Akuades Muflihah, Ahdiah Imroatul; Destiawan, Rian Anggia; Wijaya, Anas Fadli; Widia, Leny Yulia; sufi, Queen Nurul; Azizah, Laila Camelia Nur; Makki, Alisa Alfiya
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 1 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i1.1470

Abstract

Giemsa staining is a microscopic staining technique that aims to identify the morphology of leukocyte cell types. One of the methods that uses giemsa staining is the method of examining peripheral blood smear preparations which aims to evaluate the calculation of leukocyte type. The dyeing quality of giemsa is greatly influenced by the type of diluent material, namely buffer and aquadest. This study aims to determine the morphological picture of neutrophil cells in giema staining with time variations in aquadest diluent solution. The method of this study was to take blood from adults aged 20-25 years and had no history of illness as many as 24 samples. It was divided into 2 dilution groups, namely pH buffer 6.8 and aquadest, where each dilution was divided into 4 groups, namely group 1 soaking the peripheral blood smear preparation for 10 minutes, group 2 soaking the peripheral blood smear preparation for 20 minutes, group 3 soaking the peripheral blood smear preparation for 30 minutes, group 4 soaking the peripheral blood smear preparation for 40 minutes. Then a qualitative analysis was carried out, namely by comparing the background of the preparation and the morphology of neutrophil cells microscopically in each group. The results of this study are that microscopically groups 1 and 2 have a clear preparation background, while in groups 3 and 4 the preparation background is dirty and there are neutrophil granules and morphology in groups 1 and 2 show dark blue neutropnhil cells, groups 3 and 4 show purple and bluish-purple neutrophil cells. The conclusion of this study is based on the background of the preparation, and the morphology of neutrophil cells, the group that has the best results is groups 1 and 2. Key words : Aquadest, eosinophil cells, eutrophil cells, Giemsa staining
Pemeriksaan Kesehatan Dan Edukasi Kesehatan Masyarakat Desa Kamal Dalam Upaya Mengontrol Kadar Gula Darah Aggustin, Ayu Tri; Wijaya, Anas Fadli; Muflihah, Ahdiah Imroatul; Hidayati, Sholihatil
PRAXIS: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 3 (2024): PRAXIS
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47776/praxis.v2i3.943

Abstract

This community service activity is a form of effort to support the government program "Healthy Communities that are Independent and Just. The target subjects for partners in this community service activity are the people in Kamal Village, Arjasa District, and Jember Regency. Preliminary studies have been carried out and show that the incidence rate in the village is very high. Thus, health checks and health promotion in an effort to control blood sugar levels through the use of natural germplasm are very necessary. The health checks that will be carried out include random blood sugar checks (GDS). The education provided can include understanding the causes, signs and symptoms, risk factors, and types of healthy nutrition to prevent and control complications of diabetes mellitus. Early screening can be done by carrying out laboratory examinations in the community. After the examination, it was discovered that some people experienced hyperglycemia and some experienced hypoglycemia. Through this activity, the public can understand the material presented and can find out their health status so that early prevention can be carried out.
Systematic Review: Tantangan Pelayanan Pengobatan Pasien TB Saat Pandemi Covid-19 Muflihah, Ahdiah Imroatul; Martha, Evi
Jurnal Kesehatan Vol 13 No 1 (2022): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v13i1.2565

Abstract

The Covid-19 pandemic is affecting global health issues. The interaction between Covid-19 patients and Tuberculosis makes it the number one cause of death worldwide. Numerous deaths caused by Covid-19 are reported every day in Indonesia, leading to a reduced number of TB patients who require routine treatment to health services in the community. Therefore, this review aimed to identify challenges in treatment services for TB patients during the Covid-19 pandemic. The study design used was a systematic review to identify the challenges in treatment services for TB patients during the Covid-19 pandemic. The challenges are a low-capacity health system, lack of knowledge about health, less-optimum treatment for TB patients due to social restrictions, limited regular visits to health services, and low TB case detection. To prevent negative impacts in reduced treatment for TB patients, telemedicine can be used, proper knowledge can be provided through social media, and consistent policies can be implemented to strengthen the weakening health system.
Immunomodulator Effect of Cnidoscolus aconitifolius Leaves Extract on CD4+ and CD8+ Expression in Salmonella typhimurium infected mice Hidayati, Sholihatil; Susanti, Dhina Ayu; Destiawan, Rian Anggia; Muflihah, Ahdiah Imroatul; Handayani, Rizka; Anggitasari, Wima
Pharmaciana Vol. 14 No. 1 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i1.27050

Abstract

Typhoid fever is a common health problem in the community caused by Salmonella bacteria. The incidence rate of this infection will increase if a person's immune system is weakened. Plant extracts are generally considered to be potential immunomodulatory agents developed, which have smaller side effects. Research shows that Cnidoscolus aconitifolius leaves have medicinal properties including as hepatoprotective, antidiabetic, and anticardiovascular. The results of the antioxidant activity test show the results that Cnidoscolus aconitifolius leaves extract (CAE) has potential as an antioxidant with a very strong category. This study was conducted to determine the effect of giving Japanese papaya leaf extract on CD4+ and CD8+ expression in Babl/c mice induced by Salmonella typhimurium bacteria. The study was started by preparing 70% ethanol extract from Cnidoscolus aconitifolius leaves and preparing 30 Babl/C mice as experimental animals which were divided into 6 groups (healthy control, negative control, positive control and treatment group by giving CAE dose of 100 mg/kgbw, 200 mg/kgbw and 400 mg/kgbw). Induction was carried out by oral infection with Salmonella thypimurium bacteria. After 3 days the infected mice were treated orally once a day for 7 days. Evaluation of CD4+ and CD8+ expression was carried out using the flowcytometer method of the lymph organs. Data analysis was carried out by the Anova test followed by the post hoc test (Tukey) using the SPSS for Windows application. The results showed that giving CAE at doses of 100 mg/kgbw, 200 mg/kgbw and 400 mg/kgbw could increase the expression ratio of CD4+ and CD8+, whereas giving CAE at a dose of 400 mg/kgbw showed significantly different results (p<0.05) to the negative control. This shows that the CAE has potential as an immunomodulatory agent that can improve immune function.
Sosialisasi Peningkatan Gerakan 1 Rumah 1 Jumatik Untuk Pengendalian Demam Berdarah Dengue Mufidah, Hartalina; Agustin, Ayu Tri; Wijaya, Anas Fadli; Muflihah, Ahdiah Imroatul
Kolaborasi: Jurnal Pengabdian Masyarakat Vol 5 No 3 (2025): Kolaborasi: Jurnal Pengabdian Masyarakat
Publisher : Yayasan Inspirasi El Burhani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56359/kolaborasi.v5i3.511

Abstract

Introduction: Dengue Hemorrhagic Fever remains a significant health problem in Jember Regency. Jember Regency is among the top three districts with the highest dengue cases in East Java, reporting 781 cases, alongside Malang Regency was 1,224 cases and Ngawi Regency was 673 cases. It situation may be attributed to the lack of public awareness about maintaining a mosquito-free environment and the suboptimal implementation of the "1 House 1 Mosquito Monitor" (G1R1J). Kamal Village is one of the areas with environmental conditions conducive to mosquito breeding and the spread of Dengue Hemorrhagic Fever. Socializing the "1 House 1 Mosquito Monitor" is crucial to increasing public knowledge about the causes of Dengue Hemorrhagic Fever and the importance of Jumantik's role. Objective: The purpose of this service was promote the "1 House 1 Mosquito Monitor" and conduct screening IgM/IgG in Duplang Hamlet, Kamal Village, Arjasa District, Jember Regency Method: This public service was conducted by by socializing the definition of dengue disease, symptoms, prevention and the 1 House 1 Mosquito Monitor using leaflet. Then it was followed by IgG and IgM assay using Rapid test. The number of residents who participated was 16 people. Result: Socialization of One House One Mosquito Monitor were 3M Plus Dengue Prevention (Drain, Cover, Bury), control the mosquito breeding, and Larvacide applications. The IgG and IgM test results showed negative Conclusion: This community service was the successful socialization of the "1 House 1 Mosquito Monitor". None of them had been exposed to the dengue virus.
Effect of Lime Juice (Citrus Aurantifolia) on Blood Urea Nitrogen and Creatinine Levels in Wistar Rats Induced by Gentamicin Sufi, Queen Nurul; Mufidah, Hartalina; Muflihah, Ahdiah Imroatul; Hidayati, Sholihatil
Biomedika Vol 17, No 1 (2025): Biomedika Februari 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Gentamycin is an antibiotic that has the effect of acute kidney failure. Increased levels of BUN and Creatinine can characterize acute kidney failure. Lime juice (Citrus aurantifolia) contains flavonoid compounds as antioxidants that can regenerate kidney function. This study aims to determine the difference in BUN and Creatinine levels before and after being given lime juice. This study uses an experimental study design with a pretest-postest design. In the model mice, gentamicin was induced at a dose of 60 mg/KgBB for 10 days, and then BUN and creatinine levels were checked. This study was divided into 5 groups, including the negative, healthy, and treatment groups 1, 2, and 3, with a dose of lime juice 0.5 ml/KgBB, 1.0 ml/KgBB, and 1.5 ml/KgBB. The results showed that the average change in BUN levels before and after between groups was significant (sig < 0.05), and the average change in creatinine levels before and after between groups was significant (sig < 0.05). This study concluded that the average decrease in BUN levels in treatment group 3 was significant by 7.9 mg/dl, and the average decrease in creatinine levels in treatment groups 2 and 3 was significant by 0.8 mg/dl.
Co-Authors Aggustin, Ayu Tri Agustin, Ayu Tri Anas Fadli Wijaya Azizah, Laila Camelia Nur Destiawan, Rian Anggia Evi Martha Hidayati, Sholihatil Makki, Alisa Alfiya Mufidah, Hartalina Rizka Handayani sufi, Queen Nurul Susanti, Dhina Ayu Widia, Leny Yulia Wima Anggitasari