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All Journal Jurnal Analis Kesehatan Jurnal Kesehatan dr. Soebandi
Muflihah, Ahdiah Imroatul
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Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Analisis Pemantapan Mutu Internal Pada Pemeriksaan Trombosit Dan Leukosit Menggunakan Metode Sigma Metric Di Puskesmas X Muflihah, Ahdiah Imroatul; Muhammad Wildanun Ulum; Anas Fadli Wijaya; Hartalina Mufidah
Jurnal Analis Kesehatan Vol. 14 No. 2 (2025): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v14i2.5458

Abstract

Pemantapan mutu internal (PMI) di laboratorium krusial dilakukan untuk pengawasan dan pencegahan kesalahan hasil pemeriksaan, salah satu PMI dilakukan pada pemeriksaan trombosit dan leukosit menggunakan hematology analyzer untuk pencegahan kesalahan baca. Penelitian ini bertujuan untuk menganalisis hasil kontrol kualitas berapa akurasi, presisi, grafik Levey Jenning dengan aturan Westgard dan Sigma Metrik. Metode pada penelitian ini menggunakan metode deksriptif kuantitatif dengan menggunakan data sekunder pemeriksaan kontrol trombosit dan leukosit pada bulan Juli - Oktober 2024. Data kontrol pemeriksaan trombosit dan leukosit dianalisis berdasarkan aturan Westgard dan Sigma Metrik. Hasil pemantapan mutu internal pada penelitian ini menunjukkan bahwa keduanya memiliki tingkat akurasi yang baik karena berada dalam batas bias ±10%. Nilai presisi memenuhi kriteria karena tidak keluar dari nilai CV >5%. Evaluasi grafik Levey–Jennings berdasarkan aturan Westgard tidak menunjukkan pelanggaran aturan. Nilai Sigma Metrik untuk trombosit berada pada kategori sangat baik (>6), sedangkan nilai sigma metrik untuk leukosit berada pada kisaran >2 yang masih dapat dinilai layak untuk digunakan dalam pemeriksaan sampel.
Effect of Telang Flower Infusion on Lymphocyte Cells and Neutrophil Cells (Segment And Band) in Microplastic-Induced Rats Muflihah, Ahdiah Imroatul; Destiawan, Rian Anggia; Sari, Leny Yulia Widia
Jurnal Kesehatan dr. Soebandi Vol 14 No 1 (2026): Jurnal Kesehatan dr. Soebandi
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jkds.v14i1.1057

Abstract

Background:   Microplastics causing tissue damage and increasing the number of lymphocyte cells and neutrophils (segment and band). Telang flower infusion contains flavonoids as antioxidants that can as immunomodulatory. The purpose of this study was to determine the effect of telang flower infusion on the number of lymphocyte cells, band and segment neutrophils. Methods:   Methode begins with the manufacture of telang flower infusion, the manufacture of microplastics (heating bottled drinking water at a temperature of 65oC), the analysis of the size of microplastics using electron microscopes, the analysis of microplastic types using the FTIR method, the grouping of negative control, positive control, and treatment with a dose of 4 ml/250g BW, exposure to microplastics, blood smear, giemsa staining for lymphocyte and neutrophil cell analysis, and statistical test using One-Way ANOVA (p<0.05) with Tuckey HSD (p<0.05). Results:  The results showed that the average number of negative control band neutrophil cells (3.50+4.50), positive control (3.17+3.81), and treatment (5.33+3.61) and the One-Way ANOVA analysis showed values (p=0.81>p=0.05), the average segment neutrophil cells of the negative control (23.67+6.80), the positive control (21.33+5.82), the treatment (24.00+8.19),  and the One-Way ANOVA analysis showed values (p=0.776>p=0.05), negative control lymphocyte cells (68.50+14.84), positive control (72.17+4.99), and treatment (64.00+12.10). The One-Way ANOVA analysis showed a value of (p=0.481>p=0.05). Conclusions:  It can be concluded that telang flower infusion has a tendency to act as an immunomodulator at the biological level characterized by a decrease in lymphocyte cells, an increase in band neutrophil cells and segment nuetrophic cells.
Co-Authors Anas Fadli Wijaya Destiawan, Rian Anggia Hartalina Mufidah Muhammad Wildanun Ulum Sari, Leny Yulia Widia