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All Journal Jurnal Biosains Pascasarjana Jurnal Penelitian Kesehatan Suara Forikes Jurnal Kesehatan Prima Indonesian Journal of Global Health research Medicra (Journal of Medical Laboratory Science/Technology) Anakes: Jurnal Ilmiah Analis Kesehatan JURNAL BIOSHELL Jurnal Pengabdian Masyarakat (ABDIRA) Jurnal EduHealth Jurnal Kesehatan Kolaborasi Jurnal Pengabdian Masyarakat Prisma Sains: Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Jurnal Pengabdian Masyarakat Waradin Jurnal Kesehatan Amanah Jurnal Pengabdian kepada Masyarakat Innovative: Journal Of Social Science Research Jurnal Pengabdian Kepada Masyarakat: Kesehatan (JPKMK) Faedah: Jurnal Hasil Kegiatan Pengabdian Masyarakat Indonesia Jurnal Pengabdian Masyarakat Kesehatan Terkini
Anas Fadli Wijaya
Universitas Dr. Soebandi
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Regulasi Reseptor Cytotoxic T Lymphocyte Associated Protein 4 Limfosit T Terhadap Kanker dan Autoimun: Literature Review Rian Anggia Destiawan; Anas Fadli Wijaya; M Elyas Arif; Septyana Eka Rahmawati
Jurnal Biosains Pascasarjana Vol. 23 No. 2 (2021): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v23i2.2021.49-54

Abstract

Cytotoxic T Lymphocyte Associated Protein 4 (CTLA-4) adalah reseptor limfosit T yang berikatan dengan B7-1 dan B7-2 dari sel dendritik. CTLA-4 berperan menghambat kerja limfosit T. CTLA-4 diekspresikan oleh limfosit T pada fase istirahat atau setelah teraktivasi. CTLA-4 ditemukan pada sel dendritik, monosit, limfosit B, limfosit T, granulosit, dan stem sel CD34+. CTLA-4 berperan penting dalam perkembangan tumor, kanker serta autoimun. Literatur review ini bertujuan untuk membahas regulasi CTLA-4 limfosit T terhadap penyakit kanker dan autoimun. Metode: pencarian sistematis pada tiga database yaitu NCBI, science direct, dan nature dengan kata kunci “CLTA-4 and T cell and cancer and autoimmune” tahun 2021. Hasil: didapatkan 6 pilihan intervensi untuk mengurangi ekspresi CTLA-4 dan 1 intervensi untuk meningkatkan ekspresi CTLA-4. Kesimpulan: berdasarkan analisis terhadap 7 artikel penelitian, penurunan ekspresi CTLA-4 dapat menghambat pertumbuhan kanker dan peningkatan ekspresi CTLA-4 dapat menghambat berkembangnya autoimun maka dapat. 
NATURAL MEDIA FOR THE GROWTH OF CANDIDA ALBICANS CAUSES OF CANDIDIASIS BY ARTOCARPUS COMMUNIS Yunan Jiwintarum; Urip Urip; Anas Fadli Wijaya; Maruni Wiwin Diarti
Jurnal Kesehatan Prima Vol 11, No 2 (2017): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v11i2.10

Abstract

a candida albicans requires a source of nutrients especially carbohydrates that are sufficient for its growth. Semi-synthesis media is the most common medium for growing Candida albicans. Semi-synthesized media composes of natural and synthesis materials. An abundance of biological sources in nature with higher carbohydrate content than natural ingredients on semi-synthesized media encourage to find the materials of alternative media from different carbohydrate sources, particularly grains, one of them is breadnut seed. This study aimed to find out a potential of Artocarpus communis as an alternative media for the growth of Candida albicans causes of candidiasis. This study was true experiment, used 6 replications and 4 treatments with PDA as control media, the media of Artocarpus communis with concentration of 10%, 20%, and 30%. The data of Candida albicans growth was tested statistically used One Way Anova with 95% confidence level or α = 0.05. The result indicated that a number of Candida albicans growth on PDA media was 13.7 colonies, on alternative media of Artocarpus communis with the concentration of 10% was an average of 13.5 colonies, the concentration of 20% was an average of 16.3 colonies and the concentration of 30% was an average of 24 colonies. The conclusion: the Artocarpus communis can be employed as an alternative natural media of Candida albicans growth.
EFFECT OF BLACK GRAPE EXTRACT ON CD4+ and CD8+ EXPRESSION IN MICE INFECTED WITH Salmonella typhimurium rian anggia destiawan; septyana eka rahmawati; anas fadli wijaya; Ahdiah Imroatul Muflihah; Mutia Hariani Nurjanah; Sholihatil hidayati; Ni Ketut Yuliana sari
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.54-63

Abstract

Salmonelosis is a disease caused by the Salmonella sp. that causes a decrease in CD4+ expression. Black grape can boost the immune system through CD4+/CD8+ proliferation. The purpose of this study was to evaluate the activity of black grape extract to CD4+ and CD8+ expression in mice infected with Salmonella typhimurium. Research method is extract of black grapes, Salmonella typhimurium 108 infection, bacterial evaluation, extract therapy, flowcytometery examination, and 95% Anova test. The results of Anova test showed that the expression of CD4+ and CD8+ is not different. Biologically, an increase in CD4+ and CD8+ expression at doses of 100 mg/KgBB. A decrease in CD4+ and CD8+ expression at doses of 200 mg/KgBB and 400 mg/KgBB. Conclusion, biologically, black grape extract can increase the expression of CD4+ and CD8+ at low doses, as well as may decrease the expression of CD4+ and CD8+ at moderate to high doses.
Antimicrobial Test of Telang Flower Extract (Clitoria ternatea L.) against the Growth of Candida albicans Anas Fadli Wijaya; Rian Anggia Destiawan; Nosa Ika Cahyariza
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol 11, No 1: January 2023
Publisher : IKIP Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/j-ps.v11i1.6857

Abstract

Candidiasis is an acute and subacute fungal infection caused by the Candida group, especially Candida albicans. Infectious diseases due to microbes can be treated using modern drugs, namely antifungals. In some cases, antimicrobials often develop resistance due to improper administration. The occurrence of such resistance encourages further research on new active ingredients that can be used as antimicrobial alternatives, especially from plant sources containing flavonoids. The purpose of this study is to determine the effect of antimicrobial content of ethanol extract 70% of Telang flowers on the growth of C. albicans. This research is a laboratory experimental research with post test control group design only method. Determination of antimicrobial activity is carried out by the pitting method. Telang flower extract that has been diluted with 10% DMSO, taken as much as 30µl then dripped into the well. Positive control using antimicrobial ketocenazole and negative control using DMSO 10% which was dripped on the well as much as 30µl. Based on the results of observations, the inhibition zone is only formed in positive control with an average of 15.5 + 0.4 mm. The results of ethanol extract of 70% Telang flowers that have been conducted did not show antimicrobial activity against fungal growth C. albicans. The absence of this inhibitory zone is due to the unknown concentration of active compounds of Telang flowers that are responsible as antimicrobials, so it is necessary to conduct research on the search for concentrations of active compounds that have the ability as antimicrobials.
UJI EFEKTIVITAS JENIS MASKER BERDASARKAN PERTUMBUHAN BAKTERI PADA MEDIA NUTRIENT AGAR Anas Fadli Wijaya; Nosa Ika Cahyariza; Rian Anggia Destiawan
Jurnal Kesehatan Vol. 10 No. 1 (2022): April : Health Journal “Love That Renewed”
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55912/jks.v11i1.109

Abstract

Mikroorganisme patogen dapat tersuspensi dalam arus udara dan dapat menempuh jarak yang cukup jauh. Pencegahan terjadinya infeksi melalui udara dapat dilakukan dengan berbagai cara, salah satunya penggunaan masker. Tujuan dari penelitian ini adalah untuk mengetahui efektifitas berbagai variasi masker berdasarkan jumlah pertumbuhan bakteri pada media nutrient agar. Penelitian ini memiliki sifat eksperimental laboratorium dengan desain yang digunakan adalah post test control group design only. Dari ketiga masker uji yang digunakan (surgical masker, masker kain, dan KN95) tidak ditemukan adanya pertumbuhan bakteri pada media nutrient agar setelah diinkubasi 1x24 jam, bakteri hanya tumbuh pada kontrol yaitu hembusan tanpa masker dengan jumlah pertumbuhan 3 ± 0,7 koloni.
Media Alternatif Tepung Terigu Tinggi Protein Untuk Pertumbuhan Jamur Escherichia coli Anas Fadli Wijaya; Rian Anggia Destiawan
Jurnal Kesehatan Vol. 8 No. 2 (2020): Oktober : Health Journal “Love That Renewed”
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55912/jks.v8i2.143

Abstract

Escherichia coli infection is caused by contaminated food and drinking water, or direct contact with someone who is sick or with animals carrying the bacteria. Diagnosis of diseases caused by E. coli can be done in several ways, but in general it is done clinically, then strengthened by microscopic examination and culture. Commercial media that is often used for culturing bacteria is Nutrient Agar (NA). NA media is a synthetic medium with a high content of carbohydrates and proteins that support bacterial growth. One ingredient that has a high content of protein and carbohydrates is high-protein wheat flour. The purpose of this study was to determine the potential of high-protein wheat flour as an alternative medium for the growth of E. coli bacteria. This study was a laboratory experimental study using the post test control group design only method using 16 replications and 2 treatments, namely NA medium as a control and high protein wheat flour as an alternative medium. The results showed that the average number of E. coli growth on NA media was 336.5 colonies while on alternative high-protein wheat flour media it was 15.19 colonies. The value of p = 0.000 with a 2-way significance level (α = 0.05) then p <α. So there is a significant difference between the number of E. coli bacteria colonies in NA media and alternative high protein wheat flour media. Alternative medium for high-protein wheat flour takes a very long time to grow E. coli bacteria so it is not maximally used as an alternative medium for the growth of E. coli.
Pengaruh Penambahan Fruktosa Pada Media Potato Dextrose Agar (PDA) Terhadap Pertumbuhan Jamur Candida Albicans Anas Fadli Wijaya; Rian Anggia Destiawan
Jurnal Kesehatan Vol. 8 No. 1 (2020): April : Health Journal “Love That Renewed”
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55912/jks.v8i1.144

Abstract

Candida is a fungal infection caused by one of the Candida species, the most common being Candida albicans. Diagnosis of candidiasis due to C. albicans can be done in various ways, but the gold standard for examination is by culture. Specimen culture is carried out by planting on mushroom media. Potato Dextrose Agar (PDA) media is the most commonly used medium for culturing C. albicans. The amount of nutrient content in the media affects the level of fertility and incubation time of the microbes grown on the media. In PDA media, the type of sugar (dextrose) used for nutrition is monosaccharide. There are many types of monosaccharides, one of which is fructose. The purpose of this study was to determine the effect of adding fructose to PDA media on the growth of C. albicans. This study was a laboratory experimental study using the post test control group design only method using 16 replications and 2 treatments, namely PDA media without the addition of fructose and PDA media with the addition of fructose. The results showed that the average number of C. albicans growth on PDA media without the addition of fructose was 376.56 colonies while on PDA media with the addition of fructose it was 289.19 colonies. The value of p = 0.000 with a 2-way significance level (α = 0.05) then p <α. So there is a significant difference between the number of C. albicans fungal colonies on PDA media with and without the addition of fructose. The addition of fructose causes the growth of C. albicans to be slower than without the addition of fructose.
Media Alternatif Tepung Maizena Untuk Pertumbuhan Jamur Trichophyton rubrum Anas Fadli Wijaya; Rian Anggia Destiawan
Jurnal Kesehatan Vol. 9 No. 2 (2021): Oktober : Health Journal “Love That Renewed”
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55912/jks.v9i2.145

Abstract

Infectious diseases due to microbes in Indonesia are mostly caused by fungal infections. One of these microbes is Trichophyton rubrum. There are several ways to diagnose dermatophytosis, but in general it is done clinically, then confirmed by microscopic examination and culture. The commercial media that is often used for culturing mushrooms is Potato Dextrose Agar (PDA). PDA media is a semi-synthetic medium with a high carbohydrate content. One ingredient that contains high carbohydrates is cornstarch. The purpose of this study was to determine the potential of cornstarch as an alternative medium for the growth of the fungus T. rubrum. This research is a laboratory experimental study with the post test control group design only method using 16 replications and 2 treatments, namely PDA media as a control and cornstarch media as an alternative medium. The results showed that the average number of T. rubrum growth on PDA media was 163.94 colonies while on alternative media cornstarch was 35.51 colonies. The value of p = 0.000 with a 2-way significance level (α = 0.05) then p <α. So there is a significant difference between the number of T. rubrum fungus colonies on PDA media and cornstarch alternative media. Cornstarch alternative media can be used as an alternative medium for the growth of T. rubrum but not as optimal as PDA media.
Peningkatan Pengetahuan dan Skrining Penyakit Infeksi dan Degeneratif: Tuberkulosisi dan Diabetes Melitus: Increased Knowledge and Screening of Infectious and Degenerative Diseases: Tuberculosis and Diabetes Mellitus Wijaya, Anas Fadli; Destiawan, Rian Anggia; Agustin, Ayu Tri
Jurnal Pengabdian Kepada Masyarakat: Kesehatan Vol. 3 No. 4 (2023): Desember
Publisher : Sekolah Tinggi Ilmu Kesehatan Notokusumo Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstrak: Kabupaten Jember merupakan salah satu kabupaten yang masih memiliki masalah terhadap penyakit tuberkulosis dan diabetes melitus di Indonesia, sehingga perlu adanya edukasi dan pemeriksaan terhadap penyakit tuberkulosis dan diabetes melitus kepada masyarakat.  Pengabdian masyarakat ini  bertujuan  untuk  untuk meningkatkan pengetahuan dan skrining penyakit tuberkulosis dan diabetes melitus. Metode yang digunakan yaitu  metode Participation Action Research  (PAR)  melalui  kegiatan perencanaan, intervensi (ceramah,   diskusi   dan   pemeriksaan)terkait  penyakit tuberkulosis dan diabetes melitus dan evaluasi. Setelah dilakukan pemberian edukasi kepada respondens, terdapat peningkatan pengetahuan masyarakat terhadap penyakit tuberkulosis dan diabetes melitus yaitu mencapai 100% dan dari hasil skrining melalui pemeriksaan darah didapatkaan rerata masyarakat yang memiliki penyakit diabetes melitus sebesar 16,7% dari total responden.   Abstract: Jember Regency is one of the districts that still has problems with tuberculosis and diabetes mellitus in Indonesia, so there is a need for education and examination of tuberculosis and diabetes mellitus to the community.  This community service aims to increase knowledge and screening of tuberculosis and diabetes mellitus. The method used Participation Action Research (PAR) method through planning, intervention (lectures, discussions and examinations) related to tuberculosis and diabetes mellitus and evaluation. After providing education to respondents, there was an increase in public knowledge about tuberculosis and diabetes mellitus, reaching 100% and from the results of screening through blood tests, the average number of people who had diabetes mellitus was 16.7% of the total respondents.
Gambaran Morfologi Sel Neutrofil Pada Pewarnaan Giemsa dengan Variasi Waktu Pada Larutan Pengencer Akuades Muflihah, Ahdiah Imroatul; Destiawan, Rian Anggia; Wijaya, Anas Fadli; Widia, Leny Yulia; sufi, Queen Nurul; Azizah, Laila Camelia Nur; Makki, Alisa Alfiya
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 1 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i1.1470

Abstract

Giemsa staining is a microscopic staining technique that aims to identify the morphology of leukocyte cell types. One of the methods that uses giemsa staining is the method of examining peripheral blood smear preparations which aims to evaluate the calculation of leukocyte type. The dyeing quality of giemsa is greatly influenced by the type of diluent material, namely buffer and aquadest. This study aims to determine the morphological picture of neutrophil cells in giema staining with time variations in aquadest diluent solution. The method of this study was to take blood from adults aged 20-25 years and had no history of illness as many as 24 samples. It was divided into 2 dilution groups, namely pH buffer 6.8 and aquadest, where each dilution was divided into 4 groups, namely group 1 soaking the peripheral blood smear preparation for 10 minutes, group 2 soaking the peripheral blood smear preparation for 20 minutes, group 3 soaking the peripheral blood smear preparation for 30 minutes, group 4 soaking the peripheral blood smear preparation for 40 minutes. Then a qualitative analysis was carried out, namely by comparing the background of the preparation and the morphology of neutrophil cells microscopically in each group. The results of this study are that microscopically groups 1 and 2 have a clear preparation background, while in groups 3 and 4 the preparation background is dirty and there are neutrophil granules and morphology in groups 1 and 2 show dark blue neutropnhil cells, groups 3 and 4 show purple and bluish-purple neutrophil cells. The conclusion of this study is based on the background of the preparation, and the morphology of neutrophil cells, the group that has the best results is groups 1 and 2. Key words : Aquadest, eosinophil cells, eutrophil cells, Giemsa staining
Co-Authors Ade Sucipto Aggustin, Ayu Tri Agustin, Ayu Tri AHDIAH IMROATUL MUFLIHAH Alisa Alfiya Makki Arif Santoso Azizah, Laila Camelia Nur Elnaja, Dinar Anisa Fuzianingsih, Eka Novia Hanifiyah, Fitroh Hayati, Farhatul Hidayati, Larantika Hidayati, Sholihatil Holifah, Siti Nur Humairoh, Riska Tsamarah Ikhsan, Ahmad Mufti Kamaliyah, Indy Kumalasari, Nurul Chamidah Laila Camelia Laila Camelia Nur Azizah Laksono, Eko Budi Lenny Yulia Widia Sari Leny Yulia Widia M Syaifuddin M. Elyas Arif Budiman Makki, Alisa Alfiya Maruni Wiwin Diarti Meri, Meri Mufidah, Hartalina Mutia Hariani Nurjanah Mutiurrohman, Achmad Ni Ketut Yuliana Sari Nosa Ika Cahyariza Nosa Ika Cahyariza Nurjanah, Mutia Hariani Pratikah Verdianti Purwanti, Ananda Kamila Nurcahya Putri, Diva Amalia Queen Nurul Sufi Rahma Diyan Martha Rahmawati, Ayu Sintya Citra Rahmawati, Septyana Eka RIAN ANGGIA DESTIAWAN Rian Anggia Destiawan Romaidha, Iqlila Rossa, Handita Emilia Safitri, Yunita Diyah Sholihatil Hidayati Sigit Purwanto Siswidiani, Maya Diva Siti Maimunah Sofia Soleha, Nurmaidatus sufi, Queen Nurul Sulastri Sulastri Tilarso, Dara Pranidya Urip Urip Wafa, Muhammad Ali Widayanti, Sindi Dwi Widia, Leny Yulia Yunan Jiwintarum