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All Journal BERKALA SAINSTEK Dedikasi: Jurnal Pengabdian kepada Masyarakat Journal of Biobased Chemicals (JOBC) InSTEM At Turots: Jurnal Pendidikan Islam
Istiqomah Rahmawati
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Mathematics Physics Social Sciences Other

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Evaluasi Efektivitas Kolom Biofilter dengan Media Campuran untuk Pengolahan Limbah Cair Tahu Eka, Silvia Devi Eka Putri; Mutiara Dewi Rukmana; Istiqomah Rahmawati; Tazkia Maulida Putri; Zahwa Annaya
Journal of Biobased Chemicals Vol. 5 No. 1 (2025): Journal of Biobased Chemicals
Publisher : Program Studi Teknik Kimia Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jobc.v5i1.5595

Abstract

Indonesia's increasing number of tofu industries has led to a rise in waste volume from this sector, requiring exceptional management. Tofu wastewater, a byproduct of tofu production that is no longer utilized, contains a high concentration of organic matter and can adversely affect water supplies if discharged untreated. This study aims to investigate an efficient filtration technique using filtration media for processing tofu wastewater. The biofilter column used in this study was made from a Le Minerale gallon and comprised layers of zeolite, bio balls, bearings, and activated charcoal to filter and purify the water from organic substances. The results showed that while filtration effectively reduced Total Dissolved Solids (TDS) from 995 ppm to 129 ppm after the fourth filtration, it was ineffective in neutralizing the pH of the tofu wastewater, which remained at pH 4 before and after filtration. This indicates that the wastewater remains acidic and cannot be directly discharged into the environment. Additionally, conductivity and salt levels increased after multiple filtrations, possibly due to ion release from the filter materials or saturation effects. This study reveals that the column configuration and filtration materials used were ineffective in removing acidic components from tofu wastewater. Therefore, design adjustments and filtration media selection are needed to achieve better results in treating tofu wastewater.
Strategi Pengemasan dan Pemasaran Lilin Aromaterapi dan Sabun Cuci Piring dari Minyak Jelantah Sella Violina Anggraeni; Naily Fatimatuz Zaroh; Amalia Ramadhan; Mochamad Ghaza Al Ghifari; Richa Febyola Surya Putri; Boy Arief Fachri; Bekti Palupi; Meta Fitri Rizkiana; Istiqomah Rahmawati
Dedikasi:Jurnal Pengabdian kepada Masyarakat Vol. 6 No. 1 (2025): Jurnal Dedikasi
Publisher : Universitas Jayabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31479/dedikasi.v6i1.423

Abstract

Desa Plalangan yang terletak di Kecamatan Kalisat, Kabupaten Jember, merupakan salah satu desa binaan Universitas Jember dengan potensi besar dalam pengembangan produk ramah lingkungan. Masyarakat desa ini sebelumnya telah dilatih untuk mengolah minyak jelantah menjadi sabun cuci piring dan lilin aromaterapi, namun aspek pengemasan dan pemasaran produk masih perlu ditingkatkan agar memiliki nilai jual lebih tinggi. Program pengabdian ini bertujuan untuk memberikan pelatihan strategi packaging dan marketing produk berbasis minyak jelantah agar masyarakat mampu mengembangkan usaha secara mandiri. Metode pelaksanaan meliputi pendalaman mutu produk, perancangan desain kemasan, pelatihan strategi pemasaran offline dan online, serta evaluasi tingkat pemahaman peserta. Hasil kegiatan menunjukkan peningkatan wawasan dan kreativitas warga dalam mendesain kemasan yang menarik dan fungsional, serta pemahaman mengenai cara promosi produk baik melalui penjualan langsung (face to face selling) maupun live selling di platform digital seperti TikTok. Program ini diharapkan dapat memperkuat daya saing produk lokal, meningkatkan pendapatan masyarakat, serta mendukung terwujudnya ekonomi sirkular berbasis lingkungan di Desa Plalangan.
Enhancing Biotechnology Literacy through Kombucha Tea Making Socialization at SMA Negeri 1 Giri Istiqomah Rahmawati; Bekti Palupi; Merymistika Yufrani Afred; Agus Wedi Pratama
Jurnal Inovasi Sains dan Teknologi Untuk Masyarakat Vol. 3 No. 2 (2025): November
Publisher : Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/instem.v3i2.4

Abstract

Biotechnology learning at the secondary school level is often dominated by theoretical approaches, potentially limiting students' understanding of real-life scientific applications. This community service activity aimed to implement the Tri Dharma of Higher Education through socialization and training on kombucha tea making at SMA Negeri 1 Giri, Banyuwangi. The primary objective of this program was to strengthen students' applied biotechnology literacy and foster an entrepreneurial spirit by introducing the business potential of fermented products. The implementation method employed a participatory approach involving 53 students as active participants on March 18, 2025. The activity stages included interactive material delivery regarding SCOBY (Symbiotic Culture of Bacteria and Yeast) microbiology and the health benefits of kombucha, followed by practical demonstrations covering the process from starter inoculation to fermentation. In addition to technical aspects, this activity specifically incorporated training on simple economic analysis, including calculations for raw material costs, production expenses, and the determination of competitive selling prices for a home-industry scale. The results indicated high participant enthusiasm, evidenced by active participation in discussion and quiz sessions. Students successfully gained a deep understanding of the biochemical reactions occurring during fermentation and the potential of kombucha as a functional beverage. Furthermore, the provided business insights transformed students' perspectives, highlighting the high economic value of biotechnology products. It is concluded that the integration of practical training and business insights effectively enhances students' scientific competence while supporting the implementation of the entrepreneurship theme within the Merdeka Curriculum.
Natural Sources Screening for Antimicrobial Agent of Herbs, Spices, and Extracts: A Semi-Qualitative Study Felix Arie Setiawan; Meta Fitri Rizkiana; Bekti Palupi; Istiqomah Rahmawati
JOBC Vol. 1 No. 1 (2020): Journal of Biobased Chemicals
Publisher : University of Jember

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Abstract

Indonesia has been recognized for its rich natural ingredients such as spices,herbs, and extracts for decades. Furthermore, these components also have been used as an herbal medicine for a long time. Meanwhile, the apparent capability comparison of several components of antimicrobial activity has not been updated yet. This study was conducting the comparison of antimicrobial activity for several materials such as noni (Morinda citrifolia), garlic (Allium sativum), celery (Apium graveolens), galangal (Alpinia galangal), ginger (Zingiber officinale), yellow turmeric (Curcuma longa), lime (Citrus aurantifolia), papaya (Carica papaya) leaf, betel (Piper betel) leaf, and cutcherry (Kaempferia galangal) using agar dilution method. Two types of bacteria are used for the test, namely gram-negative bacteria and gram-positive bacteria, with a total of seven bacteria. The media used were TSA (Trypticase Soy Agar) media for gram-negative bacteria and MRSA (Methicillin-Resistant Staphylococcus Aureus) media for gram-positive bacteria. This study had been conducted by using a fast screening method, which is referred to as a semi-qualitative method. Several components, such as noni, lime, and betel leaf, showed a significant result of antimicrobial activity. Otherwise, other compounds, surprisingly, could not suppress bacterial growth.
Quantitative Structure-Activity Relationship Study of Ester-Based Ferulic Acid Derivatives Against Cervical Cancer Cell (HELA) Helda Wika Amini; Istiqomah Rahmawati; Rizki Fitria Darmayanti; Boy Arief Fachri
JOBC Vol. 1 No. 1 (2020): Journal of Biobased Chemicals
Publisher : University of Jember

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Abstract

Quantitative structure-activity relationship (QSAR) has been studied for ferulic acid derivatives to determine the QSAR model able to predict anticancers. The subject of this research was a set of experimentally calculated IC50 value data of 6 ferulic acid derivatives against cervical cancer cells (HELA). QSAR analysis was based on multilinear regression calculation on fitting subset using log (1/IC50) as the dependent variable, and dipole moment, partition coefficient in the n-octanol/water, and atomic net charges of the aromatic carbons as independent variables. The values of the descriptors were obtained from semi-empirical PM3 quantum mechanics calculation. The relationship between log (1/IC50) and the descriptors was described by the result in the QSAR model. The QSAR model for ferulic acid derivatives against HELA cell lines was developed with the statistical parameters of R=0.998; R2=0.999; SE=0.00857;  and F=394. The calculated log (1/IC50) using the QSAR Hansch Model for ferulic acid derivatives has excellent agreement with the experimental data of Log (1/IC50).
Effect of Ethanol Solvents in the Extraction Process of Bioactive Compounds from Brown Seaweed (Sargassum sp.) with the Ultrasound Assisted Extraction Method Slamet Pujianto; Boy Arief Fachri; Erna Subroto; Istiqomah Rahmawati
JOBC Vol. 2 No. 2 (2022): Journal of Biobased Chemicals
Publisher : University of Jember

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Abstract

Sargassum sp., part of brown algae, is traditionally used as animal feed. Nonetheless, Sargassum contains phenolic compounds that promise to be the raw material of natural antioxidants. The work is to extract phenolic compounds from Sargassum sp. in ethanol and to investigate the effect of the process on phenol yields. Several dried Sargassum sp. are milled in a certain size and extraction using ultrasound assisted extraction with ethanol solvent. Process variables are the extraction time (30-50 minutes) and the Ratio of Sargassum sp. to ethanol which is 5:100-15:100 (b/v), and power of 170-190 watts. Phenol compounds are quantified using the error acid method. The DPPH method is performed to check the antioxidant activity. To investigate the influence of the process, surface response methods based on central composite designs are applied in this work. 153.334 mgGAE/g in 30-minute extraction conditions, 170 watts of power, and a ratio between masses and solvents of 0.05. The antioxidant activity (IC50) of Sargassum sp. extract is 87.57 ppm.
Optimization of Total Phenolic Content Analysis of Chayote (Sechium edule) using Ultrasonic-Assisted Extraction and Response Surface Methodology Syahfa Adinda Relyando; Ayu Wulandari; Ditta Kharisma Yolanda Putri; Bekti Palupi; Istiqomah Rahmawati
JOBC Vol. 3 No. 1 (2023): Journal of Biobased Chemicals
Publisher : University of Jember

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Abstract

Chayote (Sechium edule) is a plant from the Cucurbitaceae family that contains several vitamins, such as A, B, and C, and is often used for medicinal purposes. Based on phytochemical analysis, chayote extract contains bioactive compounds like phenolics. Phenolic compounds are secondary metabolites used as cosmetic and medicinal ingredients due to their ability to reduce oxidative stress. This research uses UV-Vis spectrophotometry to determine the chayote extract's total phenolic content and antioxidant activity. Chayote extract was obtained using the Ultrasonic Assisted-Extraction (UAE) method with 96% ethanol solvent. The extraction variables used were time (15, 20, and 25 minutes), amplitude (45, 65, and 85%), and solvent ratio (1:5, 1:10, and 1:15). The determination of total phenolic content was based on the Folin-Ciocalteau method. At the same time, antioxidant activity was determined using DPPH. Determining chayote extraction parameters for total phenolic content using the Response Surface Methodology with a Box-Behnken type. The optimal conditions for chayote extraction were 1.327 mg GAE/g sample for total phenolic content and 29.45% for antioxidant activity under 20 minutes of extraction time, 65% amplitude, and a 1:10 solvent ratio.
Extraction of Phenolic Active Compounds from Coffee Leaves (Coffea sp.) Using the Ultrasound-Assisted Extraction Method and Total Phenol Analysis Nur Aini Dwi Alfinaini; Boy Arief Fachri; Pratamai Shelli; Helda Wika Amini; Istiqomah Rahmawati
JOBC Vol. 3 No. 1 (2023): Journal of Biobased Chemicals
Publisher : University of Jember

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Abstract

Coffea sp. is one of the largest plantation commodities in Indonesia, especially coffee leaves containing phenolic compounds. This research was conducted to extract phenolic compounds using the Ultrasound-Assisted Extraction method with ethanol solvent, which aims to determine the optimum operating conditions of extraction and the influence of variations in amplitude, time, and ratio of solvents used. The experimental design used Design Expert 13 software with the response surface method box-Behnken design. The research variables used were amplitude variations (50%, 60%, and 70%), time (10, 20, and 30 minutes), and solvent ratios (0.1, 0.15, and 0.2 g/mL). Based on our study, these parameters affect the total phenolic content. The model equation for the total phenolic content of coffee leaves obtained is Y = 0.1349 – 0.0016 A – 0.0505 B + 0.0010 C + 0.0018 AB + 0.0043 AC – 0.0018 BC – 0.0004 A2 + 0.0178 B2 – 0.0014 C2 (R2 = 0.9758) with the optimum total phenolic content located in the 17th running of 0.209 mg GAE/g under conditions of 20 minutes, the ratio of material to solvent is 0.2 g/mL, and an amplitude of 50%.
Efficient Anthocyanin Extraction from Aqueous Mixture of Cocoa Peel using Microwave Assisted Extraction (MAE) Method Nurtsulutsiyah; Yakub Hendrikson Manurung; Istiqomah Rahmawati
JOBC Vol. 3 No. 1 (2023): Journal of Biobased Chemicals
Publisher : University of Jember

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Abstract

Anthocyanins are polyphenolic compounds that are abundant in nature, with diversity in various types of plants. This study aimed to optimize the anthocyanin extraction process from cocoa shell waste using the MAE method to obtain the optimum particle size, ingredient/solvent ratio, and extraction time to produce high anthocyanin concentrations. The optimized extraction process variables include the size of the particle (1.05; 1.25; 1.49 and 2.5 x 10-4 m), the ratio of substrate/solvent (4.5; 12.5; 62.5; 79.5 x 10-3 g/mL), time of extraction (2, 4, 10, 14 minutes) and microwave power (100, 275, 450, and 625 watts). The Design Expert vs11 program with the Box-Behnken Design Response Surface Methodology (RSM) was used in the research, and the selection of process conditions was carried out from a combination of factors that resulted in an optimal response. The relationship between variables on the modeled anthocyanin concentration response: Y=0.000486-9.98637E-07A+0.026734B0.000041C-7.58240E-07D-0.000102AB+2.48606E-07AC+2.62878E09AD-0.000539BC+0.000012BD+9.71853E08CD (A: particle size; B: cocoa shell: solvent ratio; C: extraction time; D: microwave power). The optimal response value for anthocyanin concentration was 11.85.10-4 M, and the conditions of the extraction process were the particle size in the extraction process was 0.105 mm, the ratio of cocoa peel mass/ethanol was 0,03125 g/mL, the extraction time was 2 minutes, and the microwave power was 100 W.
Turmeric Leaves Extraction (Curcuma Longa L.) as a Natural Preservative Using Ultrasound-Assisted Extraction (UAE) Method Isni Salma Salsabillah; Rossi Agnessi Pebriana; Bekti Palupi; Ditta Kharisma Yolanda Putri; Istiqomah Rahmawati
JOBC Vol. 4 No. 1 (2024): Journal of Biobased Chemicals
Publisher : University of Jember

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Abstract

Turmeric leaves (Curcuma longa L.) contain phytochemical compounds that can be utilized as natural food preservatives or bioformalin due to their antibacterial properties. The extraction method used in this study is Ultrasound-Assisted Extraction (UAE), to determine the effect of variable sample-solvent ratio, time, and particle size on total flavonoid, tannin, and alkaloid compounds, as well as to determine the optimal shelf life of fresh tuna. This study used sample-solvent ratio variables of 1:10, 1:15, and 1:20; time variables of 10, 20, and 30 minutes; and particle size variables of 60, 80, and 100 mesh. The highest total flavonoid compound was obtained in the variable sample-solvent ratio of 1:20, the particle size of 100 mesh, and the time of 20 minutes at 98.076 mg/L. The highest total tannin compound was obtained in the variable sample-solvent ratio of 1:15, the particle size of 60 mesh, and the time of 10 minutes at 41.697 mg/L. The highest total alkaloid compound was obtained in the variable sample-solvent ratio of 1:10, the particle size of 100 mesh, and the time of 20 minutes at 10.092 mg/L. The optimum curing time for tuna is 36 hours at room temperature with variable sample-solvent ratio, time, and particle size of 1:20 g/mL, 20 minutes, and 100 mesh with 20% concentration. The running has the highest flavonoid compounds, so it can be concluded that flavonoid compounds have a major effect on the preservation process of tuna.
Co-Authors Abdurrahman Auf Afred, Merymistika Yufrani Agus Wedi Pratama Alfinaini, Nur Aini Dwi Alfinaini Alfun Ni’matil Husna Amalia Ramadhan Amelia Permata Ayunda Amellyah Isna Ainaiyah Andreyan Poerwo Negoro ansori ansori AYU WULANDARI Bekti Palupi Boy Arief Fachri Dianavita Fatimah Ditta Kharisma Yolanda Putri Erna Subroto Felix Arie Setiawan Hakiki, Rizki Maulana Helda Wika Amini Hilda Huril Ainia Isni Salma Salsabillah Lutsi, Nurtsulutsiyah Meta Fitri Rizkiana Mochamad Ghaza Al Ghifari Moh. Ariz Iqramullah Mohammad Fathur Rohman Y. Mutiara Dewi Rukmana Mutiara Rengganis Nurul Putri Azhari Naily Fatimatuz Zaroh Nico Dimas Saputro Nur Aini Dwi Alfinaini Nurtsulutsiyah Pratamai Shelli Putri Agustin, Mawardhi Nabilla Ratri Sekaringgalih Relyando, Syahfa Adinda Richa Febyola Surya Putri Rima Arianti Rizki Fitria Darmayanti Rossi Agnessi Pebriana Safinatin Najjah Salma, Isni Salma Salsabillah Sekar Jasmin Azzahra Sella Violina Anggraeni Shariska Putri Devina Silvia Devi Eka Putri Slamet Pujianto Sonya Hakim Raharjo Syahfa Adinda Relyando Syeikh Maulana Bima Suci Tazkia Maulida Putri Tiana Febrianti Eka Prasetyani Tri Elok Setya Megasari Widiyanto Yakub Hendrikson Manurung Yukti Nurani Zahwa Annaya Zuhriah Mumtazah Zulkarnain