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All Journal PROSIDING SEMINAR NASIONAL Jurnal Pendidikan Jasmani Indonesia Jurnal Natur Indonesia JURNAL ILMIAH KESEHATAN KEPERAWATAN JURNAL PHOTON Jurnal Ilmiah Kesehatan Keperawatan
Saryono, M.Or. S.Pd. Jas.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Nursing Physics Public Health

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OPTIMASI PRODUKSI DAN IDENTIFIKASI SENYAWA ANTIMIKROB DARI JAMUR ENDOFIT Sporothrix sp LBKURCC43 TANAMAN DAHLIA (Dahlia variabilis) Paslun, -; Saryono, -; Jose, Chiristine
Sistem Informasi Vol 7 No 01 (2016): Jurnal Photon
Publisher : Fakultas MIPA dan Kesehatan Universitas Muhammadiyah Riau

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Abstract

Dahlia tubers contain fungus Sporothrix sp which have antimicrobial activity.The objective of this research was to determine the agitation that effect the activity of antimicrobial in malt extract broth (MEB). The result showed that agitation of 150 rpm have the antimicrobial activity against Staphylococcus aureus with 21.0 mm and Escherichia colii with 12.8 mm. The identification of function group showed the extract contained O-H, C=O and Amide with UV and IR spectroscopy.
IDENTIFIKASI ISOLAT FUNGI ENDOFIT LBKURCC43 BERDASAR SEKUENS ITS rDNA DARI UMBI TANAMAN DAHLIA (DAHLIA VARIABILIS) Hendris, Sefni; Nugroho, Titania T; Saryono, -
Sistem Informasi Vol 5 No 2 (2015): Jurnal Photon
Publisher : Fakultas MIPA dan Kesehatan Universitas Muhammadiyah Riau

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Abstract

Fungi LBKURCC43 merupakan fungi endofit yang diisolasi dari umbi tanaman dahlia berbunga ungu (Dahlia variabilis) di Padang Panjang, Sumatera Barat. Identifikasi secara morfologi isolat tersebut telah dilakukan dan hanya mengidentifikasi pada tingkat genus. Identifikasi secara molekuler dengan menggunakan DNA adalah identifikasi spesies yang lebih tepat digunakan. Sebelum dilakukan analisis filogenetik secara molekuler berdasarkan sekuens DNA ribosomal pada daerah ITS-1 dan ITS-2, dilakukan ekstraksi DNA dan amplikasi PCR ITS rDNA yang baik untuk sekuensing. DNA kromosomal diisolasi menggunakan kit Wizard Genomic Purification ex Promega Co (Madison, USA) dari sel miselia berumur tiga hari. Hasil penelitian menunjukkan bahwa DNA berhasil diisolasi dari miselia yang berumur tiga hari sebelum terbentuk spora dan jumlah yang cukup tinggi untuk PCR. DNA kromosomal fungi LBKURCC43 memiliki BM (berat molekul) 10.294 pb. ITS rDNA berhasil diamplifikasi dengan PCR menggunakan pasangan primer ITS5 dan ITS4, suhu annealing untuk 440C dan menghasilkan produk PCR dengan berat molekul 455 pb. Hasil analisis filogenetik daerah ITS-1, ITS-2 dan 5,8S rDNA dari genom fungi LBKURCC43 menunjukkan bahwa spesies dari fungi LBKURCC43 adalah Hanseniaspora uvarum dengan kemiripan identitas mencapai 97%.
PENGARUH SUHU PADA PROSES PENGOMPOSAN PELEPAH SAWIT MENGUNAKAN ISOLAT LOKAL Pseudomonas stutzeri (LBKURCC 54 DAN LBKURCC 59) Apriani, Suci; Awaluddin, Amir; Saryono, -
Sistem Informasi Vol 6 No 01 (2015): Jurnal Photon
Publisher : Fakultas MIPA dan Kesehatan Universitas Muhammadiyah Riau

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Abstract

Saat ini, pelepah kelapa sawit merupakan limbah pertanian yang belum dimanfaatkan secara optimal. Pada penelitian ini, limbah pelepah kelapa sawit dikonversi menjadi kompos dengan cara fermentasi. Proses pengomposan dilakukan menggunakan kombinasi bioaktivator lokal ditambah kotoran ayam yang berfungsi menyediakan nutrisi bagi bioaktivator. Bioaktivator sebagai starter yang digunakan merupakan kombinasi dari dua Isolat yaitu Pseudomonas stutzeri (LBKURCC 54 dan 59) yang disubkultur pada Nutrient Broth dan difermentasi selama 7 hari menggunakan media bibit. Substrat diinokulasi 10 % starter dari total bahan. Untuk mempelajari kemampuan bioaktivator lokal dalam mendegradasi pelepah kelapa sawit dilakukan variasi sebagai berikut: (1) sampel dengan dan tanpa starter (2) sampel dengan dan tanpa pembalikan. Kualitas kompos terbaik yang dihasilkan dari sampel yang menggunakan starter dengan pembalikan tiga hari sekali. Nilai rasio C/N yang merupakan indikator penentu kematangan kompos, menurun hingga akhir pengomposan dan telah sesuai dengan standar SNI yaitu 13,46.
Peran Biologi Molekuler dalam Pengembangan Praktik Keperawatan ., Saryono .
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2011: PROSEDING SEMINAR NASIONAL KEPERAWATAN PPNI JATENG
Publisher : Universitas Muhammadiyah Semarang

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Abstract

ABSTRAK Latar Belakang - Biotogi molekuler merupakan cabang dari ilmu biologi yang menelaah tentang interaksi molekul dalam benda hidup khususnya sel termasuk asam deoksiribonukleat, asam ribonukleat dan protein. Bidang kajian biologi molekuler banyak mengungkap perubahan kimiawi sel (biokimia) dan efek perbedaan genetika pada individu. Kebanyakan perawat menganggap remeh kemampuan pemahaman biologi molekuler dalam praktik keperawatan Tujuan - Review ini bertujuan untuk Mengkaji peran biologi molekuler dalam pengembangan praktik keperawatan. Metode - Metode yang digunakan dalam tulisan ini adalah gagasan ilmiah dari tinjauan lileratur. Diskusi - Pelayanan keperawatan merupakan bagian integral dari pelayanan kesehatan, yang berlandaskan pada konsep sehat-sakit. Fokus praktik keperawatan ditujukan pada upaya pencapaian kondisi yang sempurna, tidak hanya terbebas dari penyakit dan kecacatan. Dalam rangka mencapai kondisi sehat secara sempurna, berbagai teknologi molekuler seperti diagnostik enzimatik, konseling genetik, teknik bayi tabung, dan transplantasi organ telah dikembangkan. Kemajuan dalam bidang. molekuler akan meningkatkan kualitas praktik keperawatan. Temuan kasus dalam praktik keperawatan akan menciptakan inovasi dalam teknik molekuler. Praktik keperawatan menggunakan dasar molekuler untuk melakukan asuhan. kgperawatan, dan asuhan keperawatan akan menemukan kasus baru untuk mengembangka ilmu biologimolekuler. Kata Kunci - Biologi Molekuler, Praktik Keperawatan, Asuhan Keperawatan, Teknologi Molekuler
Produksi Asam Levulinat dari Inulin Umbi Dahlia (Dahlia sp. L) Menggunakan Katalis Asam Klorida Murwindra, Rosa; Linggawati, Amilia; Yanti, Pepi Helza; Awaluddin, Amir; ', Saryono
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

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Abstract

Dahlia is a floriculture plant with tuber that rich of inulin. Inulin is soluble in hot water and it will settle on cool temperature in alcohol. Extraction of result from 100 g dahlia tuber taken from Bukittinggi, West Sumatra is 4% inulin (w/w dahlia tuber). Inulin flour produced is white gray. Qualitative tests with Seliwanoff and Nelson-Somogyi methods shows that inulin positive contained of fructose and reducing sugar. Extracted result of inulin can be converted into levulinic acid using acid catalyst at high temperature. In this experiment, production of levulinic acid will bundle with variate temperature, reaction time and catalyst concentration of hydrochloride acid. The reaction of inulin convertion was done in ampul that burned in oven. Conversion result of 5% inulin to be levulinic acid on heating 150 o C hydrochloride acid concentration 0,1 M about 25 minutes was 12,33% (w/w inulin). On heating 170 o C hydrochloride acid concentration 0,1 M about 15 minutes is 26,25% (w/w inulin), while on heating 150 o C hydrochloride acid concentration 1 M about 15 minutes is 32,28% (w/w inulin). Experiment result showed that concentration of glucose, levulinic acid and formiat acid increase with increasing in reaction reaction temperature while fructose concentration decrease from 55,14 mg/mL to be 0,12 mg/mL. The longer time reaction and the higher concentration of catalyst hydrochloride acid, the lower concentration of glucose and fructose and the larger concentration of levulinic acid and formic acid.
Faktor-Faktor yang Mempengaruhi Produksi Biodiesel dari Minyak Sawit Mentah Menggunakan Katalis Padat Kalsium Karbonat yang Dipijarkan Awaluddin, Amir; ', Saryono; Nelvia, Sri; ', Wahyuni
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

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Abstract

The demand for petroleum has increased recently due to the increase of world population, industries andtransportation. Biodiesel (fatty acids methyl esters) has become attractive because of high price of petroleum,limited recourses of crude oil, and environmental concerns. Most biodiesel is produced by transesterification oftriglycerides of refined/edible type oils using methanol and homogeneous catalyst such NaOH and KOH. The useof heterogeneous calcined CaCO3 catalyst, has advantages such as the ease of phase separation betweencatalyst and biodiesel. This paper presents factors affecting the synthesis of biodiesel from crude palm using thecalcined CaCO3 catalyst . The synthesis is carried out by two steps, the acid-catalyzed pre-esterification of free-fatty acid and followed by base-catalyzed transesterification of triglycerides. A study of optimizing the reactioncondition of the esterification followed by transesterification of crude palm oil (CPO) is performed to obtainmaximum production of biodiesel. Under conditions of catalyst calcination temperature of 9000C, reactor time of1.5 hours, catalyst dosage of 1,5%, reaction temperature of 700C and methanol/oil molar ratio of 9 : 1, the oilconversion is 74,6%. The as-synthesized biodiesel meets the requirements of Indonesian National Standard (SNI)for biodiesel.
HUBUNGAN LAMA KERJA PERAWAT DENGAN KEPUASAN PASIEN PASCA BEDAH DI RUANG PERAWATAN BEDAH RS PKU MUHAMMADIYAH GOMBONG prayitno, timbul; -, saryono; -, sarwono
Jurnal Ilmiah Kesehatan Keperawatan Vol 7, No 2 (2011): JURNAL ILMIAH KESEHATAN KEPERAWATAN
Publisher : LPPM STIKES MUHAMMADIYAH GOMBONG

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Abstract

Currently, nursing care services have been changing dramatically in Indonesia, especially when giving nursing care services at hospital and community. Previously, nursing care services emphasized giving nursing procedure rather than nursing care that practicing problem solving method based on nursing process and nursing science. This situation will force nurses to work harder than previously This research is to find out the correlation between nurse’s job period with post operation patients’ satisfaction in surgery care room of PKU Muhammadiyah Gombong Hospital. It was an analytical research that used cross sectional method. There were 87 respondents used as the samples research. The variables were nurse’s job period as independent variable and post operation patients satisfaction as the dependent variable. The instrument used was questioner. The data were analyzed by using spearman Rho correlation. The finding showed that there was correlation between nurse’s job period with post operation patients’ satisfaction in surgery care room of PKU Muhammadiyah Gombong Hospital with coefficient correlation result = 0.810 by seeing the probability sig value 0.001<0.005. it can be concluded that there is correlation between nurse’s job period with in surgery care room of PKU Muhammadiyah Gombong Hospital. Keywords: nurse’s job period, post operation patients’ satisfaction.
HUBUNGAN ANTARA KEBIASAAN MENGKONSUMSI SEAFOOD DENGAN KADAR KOLESTEROL PADA TENAGA KESEHATAN DI RSUD CILACAP Prayitno, Teguh; -, Saryono; -, Giyatmo
Jurnal Ilmiah Kesehatan Keperawatan Vol 10, No 3 (2014): JURNAL ILMIAH KESEHATAN KEPERAWATAN
Publisher : LPPM STIKES MUHAMMADIYAH GOMBONG

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Abstract

PenyakitJantung Koroner (PJK) adalah penyakit jantung yang timbul akibat penyempitanpada arteri koronaria. Penyebab terbanyak dari penyempitan tersebut adalaharterosklerosis. Peningkatan prevalensi PJK tersebut dipengaruhi oleh beberapafaktor antara lain asupan lemak yang tinggi. Salah satu bahan makanan yangmemiliki kadar lemak yang tinggi adalah seafood.Asupan lemak yang tinggi tersebut akan meningkatkan kadar kolesterol. Fenomenayang penulis jumpai di kalangan tenaga kesehatan RSUD Cilacap adalah masihbanyak tenaga kesehatan di RSUD Cilacap yang memiliki kebiasaan mengkonsumsi seafood secara teratur. Mengetahui hubungan kebiasaan mengkonsumsi seafood dengan kadar kolesterol pada tenaga kesehatan di RSUDCilacap Tahun 2012.Jenis penelitian ini adalahnon eksperimental dengan desain korelasional. Populasi pada penelitian ini adalahseluruh tenaga kesehatan di RSUD Cilacap, sejumlah 329 orang. Teknik sampling yang digunakan adalah purposive sampling, sejumlah 181 orang. Ujianalisis yang digunakan adalah Uji chi square. Ada hubungan yang signifikan antara kebiasaanmengkonsumsi seafood dengan kadar kolesterol pada tenaga kesehatan diRSUD Cilacap Tahun 2012 (pv = 0,000 < a = 0,05). Kata Kunci:  Konsumsi, Seafood, Kolesterol
EFEKTIFITAS FOTOTERAPI 24 JAM DAN 36 JAM TERHADAP PENURUNAN BILIRUBIN INDIRECT PADA BAYI IKTERUS NEONATORUM yuhanidz, harlina; -, saryono; -, giyatmo
Jurnal Ilmiah Kesehatan Keperawatan Vol 7, No 1 (2011): JURNAL ILMIAH KESEHATAN KEPERAWATAN
Publisher : LPPM STIKES MUHAMMADIYAH GOMBONG

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Abstract

One of therapy to reduce the indirect bilirubin level is to use fototherapy. There are several methods to provide a phototherapy, that conducted for 24 hour and 36 hour of phototherapy. This study aimed to identify the differences in the effectiveness of phototherapy 24 hour and 36 hour to the indirect bilirubin level in icterus neonatorum  patient of  PKU Muhammadiyah Hospital Gombong . This research was a quasi-experimental study, Non randomized pre test-post test with control design. The population in this study were all patients who were treated at PKU Muhammadiyah. The amaunt of sample were 50 patient.Data was analyzed by paired t-test and independent t-test. Statistical analysis with independent t-test showed that the t value > t table  (2,741 >1.71), its mean there was a difference between phototherapy 24 hour and 36 hour significantly to indirect bilirubin level in icterus neonatorum patient at RSU PKU Muhammadiyah  Gombong. The 36th hour of phototherapy was more efectifity to reduce indirect billirubin level in icterus neonatorum patient.   Keywords: 36 hour , 24 haur, phototherapy, efectivity.
FAKTOR-FAKTOR YANG MEMPENGARUHI MINAT WANITA USIA SUBUR MEMILIH METODE KONTRASEPSI MOW (METODE KONTRASEPSI WANITA) DI DESA BUTUH setyo, dhini hariyo; -, saryono; Iswati, Ning
Jurnal Ilmiah Kesehatan Keperawatan Vol 7, No 2 (2011): JURNAL ILMIAH KESEHATAN KEPERAWATAN
Publisher : LPPM STIKES MUHAMMADIYAH GOMBONG

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Abstract

Increasing the number of residents is a major issue for countries in the world, especially developing countries like Indonesia. One effort that needs to be done by the government to handle this problem is to encourage and reactivate the Indonesian national program of family planning for. A secure contraception is more effective contraceptive than other contraceptives, but users are still very particular in Butuh Village, Purworejo District. The objective of this research is to find out the factors affecting fertile women interest in choosing tubectomy contraception methods in the Butuh Village Purworejo District.This study was an analytical research with cross sectional approach. The data were collected by using questionnaires. There were 31 respondents taken as the  samples by using purposive sampling. This research show that by using  chi square correlation statistics there are a correlation between the number of children factor (p = 0.010), economic factor (p = 0.002), and socio-cultural factors (p = 0.035) with the interest of fertile women and there is no correlation between knowledge factor (p = 0.778) with fertile women interests in choosing tubectomy contraceptive methods. There are correlation between the number of children, economic, social and cultural with and there is no correlation between knowledge factors with fertile women interests in choosing tubectomy contraceptive methods. Keywords: Family planning, MOW, fertile woman
Co-Authors ', Wahyuni Amilia Linggawati Amir Awaluddin dadi santoso Desi Puspita Sari, Desi dhini hariyo setyo Eka Riyanti Fitri Rohyani giyatmo - harlina yuhanidz Indra Hermawan isma yuniar Jose, Chiristine Ning Iswati Paslun, - Pepi Helza Yanti Rakhmayanto - Rakhmayanto -, Rakhmayanto Rosa Murwindra, Rosa sarwono - siti nurlela Sri Nelvia Suci Apriani TATI NURHAYATI Teguh Prayitno TEGUH PRAYITNO timbul prayitno Titania Tjandrawati Nugroho Tri Sumarsih