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Widyastuti, Havida
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Analisis Serum Iron, Hemoglobin dan Sel Darah Sebagai Penanda Toksisitas Karbon Monoksida Pada Tikus yang Dipapar Rokok Filter Havida Widyastuti; Budi Santoso; Mudyawati Kamaruddin; Widyastuti, Havida
Journal of Health Vol. 12 No. 2 (2025): Journal of Health (JoH) - July
Publisher : LPPM STIKES Guna Bangsa Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.895 KB) | DOI: 10.30590/joh.v12n2.17

Abstract

Carbon monoxide (CO) is a harmful substance when inhaled in excessive amounts, leading to cellular distortions. The presence of CO in the blood forms carboxyhemoglobin, which reduces the overall oxygen-carrying capacity, potentially resulting in hypoxia. In response to hypoxia, the body compensates by stimulating the production of new erythrocytes and increasing iron levels. This study aims to analyze serum iron, hemoglobin, and blood cell levels as sensitive markers of CO toxicity. The study employed a true experimental post-test-only control group design with 25 rats divided into five groups that is one negative control group that not exposed to cigarettes and four treatment groups (1, 2, 3, and 4), which were sequentially exposed to filter cigarettes at rates of 2, 4, 6, and 8 cigarettes per day for 14 days. Spectrophotometric and impedance analysis methods were employed. Data analysis was performed using SPSS with post hoc tests. This studi shows that  significant increase compared to the negative control in hemoglobin was observed in all treatment groups, serum iron in group 4 (181.10 µg/dL), erythrocytes in group 4 (13.04x1012cells/L), and leukocytes in groups 2, 3, and 4 with mean levels of (12.4x109cells/L; 14.03x109cells/L; 15.09x109cells/L), while platelets showed no significant increase compared to the negative control. The study concluded that hemoglobin serves as one of the most sensitive markers of toxicity, demonstrating significance even at the lowest dose of cigarette smoke exposure.
IDENTIFIKASI SOIL TRANSMITTED HELMINT PADA FESES TERNAK BABI MENGGUNAKAN METODE DIRECT SLIDE Solikah, Monika Putri; Widyastuti, Havida
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3880

Abstract

Until now, parasitic diseases that infect humans and animals have not been considered in Indonesia because these diseases do not cause clinical symptoms (subclinical). Gastrointestinal disease caused by worm parasites is a systemic disease that attacks the digestive tract of cattle. Traditional pig farming methods and poor hygiene are the main reasons for the spread of zoonoses that can be transmitted from pigs to humans. This study aims to identify nematode hosts by examining worm eggs in pig feces using the direct slide method. From a total of 20 stool samples, 3 samples were positive for nematode worm eggs. A stool sample that is identified as positive for worm eggs determines the level of parasitic infestation. Worm egg detection was carried out in the laboratory of the Faculty of Health Sciences University and analyzed descriptively, then the number of worm eggs was counted to determine the prevalence of endoparasites.
Co-Authors Anggraeni, Rosmita Astuti, Tri Dyah Budi Santoso Havida Widyastuti Mudyawati Kamaruddin Solikah, Monika Putri