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All Journal Health and Medical Journal Berita Biologi
Setyawan, Ryan Haryo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Diagnostic Value of Saliva RT-PCR Test within Suspected SARS-CoV-2 Cases in Indonesia Putra, Andika Chandra; Zaini, Jamal; Ridwanuloh, Asep Muhammad; Nugroho, Herjuno Ari; Setyawan, Ryan Haryo; Idris, Idris; Setiawan, Ruby; Sushadi, Pangda Sopha; Wulandari, Ari Sulistyo; Zannati, Anky; Indriawati, Indriawati; Erdayani, Eva; Wahyuni, Wahyuni; Agustiyanti, Dian Fitria; Wisnuwardhani, Popi Hadi; Saniyyah, Zahrah; Azika, Wira Norman; Haryanto, Budi; Utomo, Ahmad Rusdan Handoyo; Ningrum, Ratih Asmana
Health and Medical Journal Vol 6, No 2 (2024): HEME May 2024
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/heme.v6i2.1494

Abstract

Introduction: The ongoing SARS-CoV-2 pandemic has profoundly emphasized the pressing need for accurate and reliable diagnostic procedures. Given the potential health risks associated with nasopharyngeal swabs, there has been growing interest in seeking alternative diagnostic mediums. In this context, our study delved into evaluating saliva as a potential diagnostic tool, simultaneously assessing its efficiency in relation to patient demographics and their exhibited clinical symptoms. Methods: Spanning from May to December 2020, we conducted a comprehensive cross-sectional analysis. We meticulously examined medical records to gather insights on patient characteristics, existing health conditions, onset of symptoms, clinical manifestations, and compared the results obtained from both salivary and nasopharyngeal RT-PCR tests for SARS-CoV-2. Results: Among the individuals suspected of SARS-CoV-2 infection, the mean age stood at 52.4 years, with males representing 60.3% of this group. Interestingly, a significant 76.9% reported underlying health conditions, predominantly hypertension and diabetes. The most commonly reported symptoms encompassed respiratory challenges, notably coughing and shortness of breath, succeeded by symptoms like nausea, fever, and a general sense of fatigue. The performance of saliva tests, in terms of accuracy, appeared to be significantly influenced by the timing of symptom emergence. Conclusion: The RT-PCR tests utilizing saliva samples demonstrated considerable promise, especially during the early stages of symptom manifestation, providing a reliable alternative to traditional nasopharyngeal swabs. The findings suggest a superior diagnostic sensitivity when utilizing saliva during the initial phases of a SARS-CoV-2 infection.
POTENSI PREBIOTIC DARI EKSTRAK JAMUR TIRAM PUTIH (PLEUROTUS OSTREATUS) Setyawan, Ryan Haryo; Saskiawan, Iwan; Widhyastuti, Nunuk; Kasirah, Kasirah; Mulyadi, Mulyadi
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.806

Abstract

Nowadays, people are more aware of a healthy lifestyle and demand more functional food products. It leads to the raising of prebiotics and their health benefit, such as maintaining digestive tract health, decreasing heart disease and obesity risk, and improving the immune system. Since people need to diversify healthy food product, the pursuit of novel prebiotic ingredient which is potentially incorporated into functional food product needs to be done. One substance that has potency is β-glucan from white oyster mushroom or Pleurotus ostreatus. Accordingly, the aim of this study is to observe the prebiotic potency of white oyster mushroom extract. The study started by extracting β-glucan from white oyster mushroom powder by hot water extraction and subsequently proceeded into alkaline extraction. After that, β-glucan content of extracts and residue were measured by Megazyme® β-glucan assay kit and supplemented in glucose-free growth media to see whether it can be utilized by probiotic Lactobacillus plantarum Dad-13 and pathogen Escherichia coli InaCC B-4. After prebiotic index, prebiotic activity score, pH, and titratable acidity of each extract were compared to FOS and inulin, it showed that β-glucan from water extract of P. ostreatus has the potency to become a novel prebiotic substance. It has 37.15+1.27 g/100g β-glucan content, 1.42+0.05 prebiotic index, and 0.91+0.01 prebiotic activity score. It could be utilized by probiotic to produce organic acid, such as lactic acid as well.
Co-Authors Agustiyanti, Dian Fitria Anky Zannati Azika, Wira Norman Budi Haryanto Erdayani, Eva Herjuno Ari Nugroho, Herjuno Ari Idris Idris Indriawati, Indriawati IWAN SASKIAWAN Kasirah, Kasirah Mulyadi Mulyadi NUNUK WIDHYASTUTI Popi Hadi Wisnuwardhani, Popi Hadi Putra, Andika Chandra RATIH ASMANA NINGRUM Ridwanuloh, Asep Muhammad Saniyyah, Zahrah Setiawan, Ruby Sushadi, Pangda Sopha Utomo, Ahmad Rusdan Handoyo wahyuni wahyuni Wulandari, Ari Sulistyo Zaini, Jamal