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All Journal Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi
Setiawan, Ruby
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Screening of Bacteria Producing Asparaginase Free of Glutaminase and Urease from Hot Springs in West Sulawesi Setiawan, Ruby; Larasati, Dinar Rahmi
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.129 KB) | DOI: 10.15294/biosaintifika.v11i2.17435

Abstract

L-asparaginase catalyzes the hydrolysis of asparagine into ammonia and aspartate. It has been used in chemotherapy for patients with acute lymphoblastic leukemia. L-asparaginase presents in animal, plant and microorganism. Long-term application of this enzyme can induce neurotoxicity due to the affinity towards glutamine and urea. The aim of this research was to find new source of glutaminase and urease-free asparaginase from bacteria. Bacteria were isolated from hot springs located in West Sulawesi using R2A media. The identification was employed by amplifying 16S rRNA gene. Screening of asparaginase was conducted using asparagine as single source of Nitrogen. Out of 21 isolates, 76% were Gram-negatives from the genus of Pseudomonas, Acinetobacter, Bosea, Caulobacter, Sphingomonas and Novosphingobium, while the rest of them were Gram-positives from the genus of Mycobacterium, Brachybacterium, Rhodococcus, and Staphylococcus. Twelve isolates which showed asparaginase activity were Caulobacter flavus HS1YWS2 and HS1XWS3, Acinetobacter sp. HS2XWS5, HS2XWS6, HS2XWS8, HS2YWS11, HS2YWS12, HS2YWS13, HS2ZWS14, HS2ZWS15 and HS2ZWS16. Isolates HS1YWS2 and HS1XWS3 were free of glutaminase and urease and showed the highest activity. This study was the first report of asparaginase activity from Caulobacter flavus. This result can further be used to explore the ability of asparaginase free of glutaminase and urease to treat acute lymphoblastic leukemia.
Screening and Characterization of L-Asparaginase Free L-Glutamianse Produced by Marine Bacterial Isolates Larasati, Dinar Rahmi; Setiawan, Ruby; Wijarnaka, Wijarnaka; Pujiyanto, Sri
Berkala Bioteknologi Vol. 6, No. 2, November 2023
Publisher : Berkala Bioteknologi

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Abstract

L-asparaginase (EC 3.5.1.1) is a potential pharmaceutical enzyme for ALL (Acute Lymphoblastic Leukemia) treatment. However, it can cause side effects due to the activity of enzyme L-glutaminase. Halophilic microorganisms might be potential source of the enzyme L- asparaginase free of L-glutaminase because of these microorganism are adapted to extreme environments which producing biocatalysts with different structures. The enzyme was screened from marine bacterial isolated from surface sea water and marine sediment. The enzyme was produced and characterized for optimum temperature, pH, and the effect of metal ions. The results showed that a total of 96 marine bacterial isolates, three isolates namely Pseudomonas stutzeri, Marinobacter nitratireducens, Vibrio neocaledonicus were detected by producing L asparaginase free L-glutaminase. The highest activity was produced by Marinobacter nitratireducens, 0.887 U/ml. Enzyme production at the 60 hours showed the highest enzyme activity 1,625 U/ml and specific activity 1,700 U/mg. The maximum L-asparaginase activity occurs at temperature 40 °C and pH 8 of Tris HCl buffer. The relative activity of enzymes decreases due to the presence of metal ion K+ 5 mM, and Mg2 +, Ni2+, Cu2+, Zn2+ 1 mM and 5 mM.
Co-Authors Larasati, Dinar Rahmi Larasati, Dinar Rahmi Sri Pujiyanto Wijarnaka, Wijarnaka