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EVALUASI METODE SEKSING NURI KEPALA HITAM (Lorius lory LINNAEUS, 1758) DI FASILITAS PENANGKARAN PUSAT PENELITIAN BIOLOGI Nugroho, Herjuno Ari; Zein, Moch. Syamsul Arifin
ZOO INDONESIA Vol 24, No 2 (2015): Desember 2015
Publisher : Masyarakat Zoologi Indonesia

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Penentuan jenis kelamin pada burung monomorfik seperti Nuri Kepala Hitam (Lorius lory) kadang sulit dilakukan, meskipun hal itu penting dalam program penangkaran. Tujuan dari studi ini untuk mengevaluasi reliabilitas dan efektifitas metode seksing untuk Nuri Kepala Hitam berdasarkan pengukuran karakter morfologi (morfometri) dan identifikasi molekuler menggunakan gen CHD1 dari kromosom seks. Analisis diskriminan diterapkan pada ukuran panjang tubuh dan culmen dari 9 spesimen museum. Fungsi yang terbentuk diaplikasikan pada 6 ekor burung hidup dan mampu mengklasifikasikan dengan benar 83,33% individu (5/6). Amplifikasi gen CHD1 mampu menentukan 100% sampel (6/6) dengan jantan menunjukkan pita DNA tunggal (ZZ) dan betina menunjukkan pita DNA ganda (ZW). Berdasarkan perbandingan dan evaluasi diantara kedua metode, metode molekular lebih akurat dan aplikatif digunakan untuk determinasi seksual Nuri Kepala Hitam di Fasilitas Penangkaran, Pusat Penelitian Biologi LIPI dibandingkan dengan metode morfometri. Penelitian ini menganjurkan bahwa teknik molekuler dapat digunakan dalam determinasi kelamin burung monomorfik.

Utilization of Garlic with and without Addition of Enzymes on Black-capped Lory (Lorius lory Linnaeus, 1758) in Captive Breeding Sari, Andri Permata; Nugroho, Herjuno Ari; Maharani, Sinta
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3090

ABSTRACTPhytogenic feed additives are plant-derived product such as herbs, spices, and essential oils used in animal feed to improve their performances and health. Garlic (Allium sativum) is well-known as a spice and herbal medicine. Garlic possesses antimicrobial activity and increase nutrient digestibility in animal. The objectives of this study were to compare the effect of supplementing garlic, exogenous enzyme and combination of both on Black-capped Lory (Lorius lory) in digestibility and bacterial count on excreta. Five Black-capped Lory were used in the study and fed with control diet (K1), control diet supplemented with garlic powder (K2), control diet supplemented with Allzyme SSF (K3), and control diet supplemented with both garlic powder and Allzyme SSF (K4). Variables observed include feed consumption, Apparent Metabolizable Energy (AME) value and bacterial count in excreta. Adding enzyme to the diet increased AME compared to control. AME value of K1 was 3,579 kcal/kg, meanwhile AME value of diet K3 and K4 were 3,690 kcal/kg and 3,619 kcal/kg respectively. Adding garlic to the diet did not give significant improvement to AME value. The highest inhibition of bacterial population was in K2 mash. Combination of allzyme and garlic addition showed decreased in bacterial number.Keywords: Allzyme SSF, AME value, bacterial population, black-capped Lory, garlic

A Preliminary Study of Aphrodisiac Property from Porcupine Tail Meat Ethanol Extract in Male Mice Agusta, Andria; Farida, Wartika Rosa; Nugroho, Herjuno Ari; Wulansari, Dewi; Anita, Syahfitri
ZOO INDONESIA Vol 26, No 1 (2017): Juli 2017
Publisher : Masyarakat Zoologi Indonesia

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This study aim to examine the aphrodisiac activity of ethanol extract from the tail meat of porcupine (Hystrix javanica F. Cuvier, 1823) that is traditionally believed by people in Java (Indonesia) could enhance male vitality and sexual perfor-mance. Twenty sexually inexperienced male mice were randomly divided into four groups and paired with artificially estrus female mice after one hour of drug an extract administration. Two doses of ethanol extract; 150 and 750 mg/kg were administered (p.o) to male mice. Sildenafil citrate was used as the positive control while 5% Tween 80 solution used as the negative control. Sexual behavior parameters such as mounting and intromission were observed for three hours of mating. Male mice treated with ethanol extract of porcupine tail meat at the dose 750 mg/kg BW showed higher mounting and intromission frequency compared to the group of ethanol extract dose 150 mg/kg. After 2 hours of admin-istration showed the highest frequency compared to all groups. However, the ethanol extract could not reduce the mount-ing and intromission latency as low as sildenafil citrated treated group. Present findings provide preliminary evidence of aphrodisiac properties from the ethanol extract of porcupine tail meat.

Analisis Kebutuhan Nutrien dan Efisiensi Penggunaan Pakan Bubur Formulasi pada Oposum Layang (Petaurus breviceps Waterhouse, 1839) Farida, Wartika Rosa; Sari, Andri Permata; Inayah, Nurul; Nugroho, Herjuno Ari
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTThe aims of the research was to analyze nutrient requirements, performance, and feed efficiency of porridge formulation on sugar gliders (Petaurus breviceps) based on daily feed intake and digestibility. The research was conducted at the Small Mammal Captivity of Zoology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI) Cibinong, Bogor. Descriptive method was used in this experiment, with 14 days of preliminary period and 70 days (10 weeks) of data collection. The research materials were 16 sugar gliders aged about 3-5 months and randomly divided into 2 treatments ie. 8 heads were given porridge formulation I (P0 - control treatment) and 8 heads were given porridge formulation II (PI) plus superworm 2 times a week. Estimation of feed digestibility done in vivo with the method of the feces total collection. Variables of this experiment are daily feed intake, nutrient requirement, total digestible nutrients (TDN), daily weight gain, and feed efficiency. The feedstuff of the porridge formulation consist of siamese banana (Musa sp.), watermelon (Citrullus lanatus), sweet potatoes (Ipomoea batatas), grape (Vitis vinifera), pear (Pyrus pyrifolia), boiled egg yolk, baby porridge, calcium, honey, boiled chicken meat, and superworm (Zophobas atratus). The results showed dry matter intake was 5.82 g/head / day or 29.31 g/head / day BB0.75 (P0) and 7.31 g/head/day or 34.16 g/head/day BB0.75 (PI). The average of body weight gain was 0.55 g / head / week (P0) and 0.73 g/head/week (PI) with feed efficiency ratio was 1.36% (P0) and 1.43% (PI). The PI treatment of porridge formulation II has improved the performance of sugar gliders in captivity.Keywords: Daily feed intake, weight gain, porridge formulation, sugar gliders, performance

PENGARUH PENAMBAHAN BUAH-BUAHAN DALAM PAKAN TERHADAP PERUBAHAN WARNA RAMBUT PADA OPOSUM LAYANG (PETAURUS BREVICEPS) JENIS CLASSIC GRAY Farida, Wartika Rosa; Sari, Andri Permata; Handayani, Tri Hadi; Inayah, Nurul; Nugroho, Herjuno Ari
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3766

ABSTRACTThis study aims to determine the possibility of changes in the hair color on young sugar gliders (Petaurus breviceps) were given additional fruits in the feed. Research has been conducted in the Small Mammal Captive Breeding of Zoology Division, Research Center for Biology - LIPI, Cibinong, Bogor. Descriptive method used in this study with 10 days of preliminary and 90 days (3 months) of data collection period. The research material used is 16 young sugar gliders aged about 2-3 months, divided into 2 groups namely group I consists of 8 sugar gliders (4 male and 4 female) whose the parent is brownish brown color, while group II consists of 8 sugar gliders (4 male and 4 females) whose the parent are gray in color. Each group received 2 feeding porridge formulations T0 (control) and T1 (T0 + kepok banana (Musa balbisiana), watermelon (Citrullus lanatus), and red dragon fruit (Hylocereus polyrhizus)). T0 feed composition consists of brown rice porridge, boiled chicken eggs, honey, calcium, and water. Sugar gliders is given live superworm (Zophobas morio) every Tuesday and every Thursday is given live crickets (Gryllidae). During the observation each pair of young sugar glider is placed in a wire enclosure (60 cm x 42 cm x 42 cm) equipped with feeding box, drinking water bottle and a hammock. The results showed that the average of dry matter intake T1> T0. KII / T0 / enclosure 6 show higher of body weight gain and feed efficiency than KI / T1 / enclosure 4. The addition of fruits into the T1 diet did not show any discoloration in hair on sugar gliders in captivity. Keywords: fruits, feed intake, hair/fur color, Petaurus breviceps

UTILIZATION OF GARLIC WITH AND WITHOUT ADDITION OF ENZYMES ON BLACK-CAPPED LORY (LORIUS LORY LINNAEUS, 1758) IN CAPTIVE BREEDING Sari, Andri Permata; Nugroho, Herjuno Ari; Maharani, Sinta
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3090

ABSTRACTPhytogenic feed additives are plant-derived product such as herbs, spices, and essential oils used in animal feed to improve their performances and health. Garlic (Allium sativum) is well-known as a spice and herbal medicine. Garlic possesses antimicrobial activity and increase nutrient digestibility in animal. The objectives of this study were to compare the effect of supplementing garlic, exogenous enzyme and combination of both on Black-capped Lory (Lorius lory) in digestibility and bacterial count on excreta. Five Black-capped Lory were used in the study and fed with control diet (K1), control diet supplemented with garlic powder (K2), control diet supplemented with Allzyme SSF (K3), and control diet supplemented with both garlic powder and Allzyme SSF (K4). Variables observed include feed consumption, Apparent Metabolizable Energy (AME) value and bacterial count in excreta. Adding enzyme to the diet increased AME compared to control. AME value of K1 was 3,579 kcal/kg, meanwhile AME value of diet K3 and K4 were 3,690 kcal/kg and 3,619 kcal/kg respectively. Adding garlic to the diet did not give significant improvement to AME value. The highest inhibition of bacterial population was in K2 mash. Combination of allzyme and garlic addition showed decreased in bacterial number.Keywords: Allzyme SSF, AME value, bacterial population, black-capped Lory, garlic

ANALISIS KEBUTUHAN NUTRIEN DAN EFISIENSI PENGGUNAAN PAKAN BUBUR FORMULASI PADA OPOSUM LAYANG (PETAURUS BREVICEPS WATERHOUSE, 1839) Farida, Wartika Rosa; Sari, Andri Permata; Inayah, Nurul; Nugroho, Herjuno Ari
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3405

ABSTRACTThe aims of the research was to analyze nutrient requirements, performance, and feed efficiency of porridge formulation on sugar gliders (Petaurus breviceps) based on daily feed intake and digestibility. The research was conducted at the Small Mammal Captivity of Zoology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI) Cibinong, Bogor. Descriptive method was used in this experiment, with 14 days of preliminary period and 70 days (10 weeks) of data collection. The research materials were 16 sugar gliders aged about 3-5 months and randomly divided into 2 treatments ie. 8 heads were given porridge formulation I (P0 - control treatment) and 8 heads were given porridge formulation II (PI) plus superworm 2 times a week. Estimation of feed digestibility done in vivo with the method of the feces total collection. Variables of this experiment are daily feed intake, nutrient requirement, total digestible nutrients (TDN), daily weight gain, and feed efficiency. The feedstuff of the porridge formulation consist of siamese banana (Musa sp.), watermelon (Citrullus lanatus), sweet potatoes (Ipomoea batatas), grape (Vitis vinifera), pear (Pyrus pyrifolia), boiled egg yolk, baby porridge, calcium, honey, boiled chicken meat, and superworm (Zophobas atratus). The results showed dry matter intake was 5.82 g/head / day or 29.31 g/head / day BB0.75 (P0) and 7.31 g/head/day or 34.16 g/head/day BB0.75 (PI). The average of body weight gain was 0.55 g / head / week (P0) and 0.73 g/head/week (PI) with feed efficiency ratio was 1.36% (P0) and 1.43% (PI). The PI treatment of porridge formulation II has improved the performance of sugar gliders in captivity.Keywords: Daily feed intake, weight gain, porridge formulation, sugar gliders, performance

PENGARUH PENAMBAHAN BUAH-BUAHAN DALAM PAKAN TERHADAP PERUBAHAN WARNA RAMBUT PADA OPOSUM LAYANG (PETAURUS BREVICEPS) JENIS CLASSIC GRAY Farida, Wartika Rosa; Sari, Andri Permata; Handayani, Tri Hadi; Inayah, Nurul; Nugroho, Herjuno Ari
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3766

ABSTRACTThis study aims to determine the possibility of changes in the hair color on young sugar gliders (Petaurus breviceps) were given additional fruits in the feed. Research has been conducted in the Small Mammal Captive Breeding of Zoology Division, Research Center for Biology - LIPI, Cibinong, Bogor. Descriptive method used in this study with 10 days of preliminary and 90 days (3 months) of data collection period. The research material used is 16 young sugar gliders aged about 2-3 months, divided into 2 groups namely group I consists of 8 sugar gliders (4 male and 4 female) whose the parent is brownish brown color, while group II consists of 8 sugar gliders (4 male and 4 females) whose the parent are gray in color. Each group received 2 feeding porridge formulations T0 (control) and T1 (T0 + kepok banana (Musa balbisiana), watermelon (Citrullus lanatus), and red dragon fruit (Hylocereus polyrhizus)). T0 feed composition consists of brown rice porridge, boiled chicken eggs, honey, calcium, and water. Sugar gliders is given live superworm (Zophobas morio) every Tuesday and every Thursday is given live crickets (Gryllidae). During the observation each pair of young sugar glider is placed in a wire enclosure (60 cm x 42 cm x 42 cm) equipped with feeding box, drinking water bottle and a hammock. The results showed that the average of dry matter intake T1> T0. KII / T0 / enclosure 6 show higher of body weight gain and feed efficiency than KI / T1 / enclosure 4. The addition of fruits into the T1 diet did not show any discoloration in hair on sugar gliders in captivity. Keywords: fruits, feed intake, hair/fur color, Petaurus breviceps

Manajemen Pemberian Pakan, Reproduksi dan Bonding pada Oposum Layang (Petaurus breviceps) di Penangkaran Farida, Wartika Rosa; Sari, Andri Permata; Nugroho, Herjuno Ari; Sofyani, Umar
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 13, No 1 (2016): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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In order to nutritional needs of the sugar glider (Petaurus breviceps) in captivity, it has been given a Leadbeater modified feed and insects (superworm and crickets) as a natural food.Â The average feed intake of Leadbeater and fruits juice was 27.5 grams per head and superworm/crickets were 4 pieces per head per week. The nutritional content of feed modification Leadbeater 19% protein; 8.8% fat; 3.5% Ca; and 0.5% P. Each pairs of sugar glider placed in wire cages (60 cm x 40 cm x 40 cm) in order to facilitate the monitoring of reproduction. The mean increase in young birth of sugar gliders from March to October 2015 was 4.75 head per month. The bonding process is done since young sugar glider aged 2 months, in order to obtain young sugar glider as a product of pet animals

Diagnostic Value of Saliva RT-PCR Test within Suspected SARS-CoV-2 Cases in Indonesia Putra, Andika Chandra; Zaini, Jamal; Ridwanuloh, Asep Muhammad; Nugroho, Herjuno Ari; Setyawan, Ryan Haryo; Idris, Idris; Setiawan, Ruby; Sushadi, Pangda Sopha; Wulandari, Ari Sulistyo; Zannati, Anky; Indriawati, Indriawati; Erdayani, Eva; Wahyuni, Wahyuni; Agustiyanti, Dian Fitria; Wisnuwardhani, Popi Hadi; Saniyyah, Zahrah; Azika, Wira Norman; Haryanto, Budi; Utomo, Ahmad Rusdan Handoyo; Ningrum, Ratih Asmana
Health and Medical Journal Vol 6, No 2 (2024): HEME May 2024
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/heme.v6i2.1494

Introduction: The ongoing SARS-CoV-2 pandemic has profoundly emphasized the pressing need for accurate and reliable diagnostic procedures. Given the potential health risks associated with nasopharyngeal swabs, there has been growing interest in seeking alternative diagnostic mediums. In this context, our study delved into evaluating saliva as a potential diagnostic tool, simultaneously assessing its efficiency in relation to patient demographics and their exhibited clinical symptoms. Methods: Spanning from May to December 2020, we conducted a comprehensive cross-sectional analysis. We meticulously examined medical records to gather insights on patient characteristics, existing health conditions, onset of symptoms, clinical manifestations, and compared the results obtained from both salivary and nasopharyngeal RT-PCR tests for SARS-CoV-2. Results: Among the individuals suspected of SARS-CoV-2 infection, the mean age stood at 52.4 years, with males representing 60.3% of this group. Interestingly, a significant 76.9% reported underlying health conditions, predominantly hypertension and diabetes. The most commonly reported symptoms encompassed respiratory challenges, notably coughing and shortness of breath, succeeded by symptoms like nausea, fever, and a general sense of fatigue. The performance of saliva tests, in terms of accuracy, appeared to be significantly influenced by the timing of symptom emergence. Conclusion: The RT-PCR tests utilizing saliva samples demonstrated considerable promise, especially during the early stages of symptom manifestation, providing a reliable alternative to traditional nasopharyngeal swabs. The findings suggest a superior diagnostic sensitivity when utilizing saliva during the initial phases of a SARS-CoV-2 infection.

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