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Epigenetik Pada Sindrom Ovarium Polikistik: Gangguan Proses Folikulogenesis Yang Melibatkan Gen Reseptor Androgen, Reseptor Follicle-Stimulating Hormone Dan Reseptor Luteinizing Hormone Arianto, Steven; Radhina, Afifa; Shari, Amalia; Jannah, Insani Fitrahulil; Sari, Mike Permata
Indonesian Journal of Health Science Vol 4 No 1 (2024)
Publisher : PT WIM Solusi Prima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54957/ijhs.v4i1.683

Abstract

Sindrom Ovarium Polikistik (SOPK) merupakan salah satu gangguan hormonal yang dialami oleh wanita. Gangguan ini menyebabkan disregulasi proses neuroendokrin dan mengganggu proses pembentukan folikel (folikulogenesis). Etiologi gangguan folikulogenesis pada Sindrom Ovarium Polikistik disebabkan oleh reseptor androgen, reseptor follicle stimulating hormone (FSH), dan reseptor luteinizing hormone (LH). Beberapa pendekatan ilmiah digunakan untuk memahami penyebab gangguan folikulogenesis pada Sindrom Ovarium Polikistik, salah satunya epigenetik. Faktor epigenetik seperti metilasi DNA dan modifikasi histon diduga menjadi salah satu penyebab gangguan pengaturan sinyal luteinizing hormone dan follicle stimulating hormone pada folikulogenesis. Sinyal luteinizing hormone dan follicle-stimulating hormone diatur oleh reseptor luteinizing hormone dan reseptor follicle stimulating hormone yang memiliki ekspresi messenger RNA (mRNA) yang berbeda dibandingkan dengan kondisi normal. Reseptor luteinizing hormone diekspresikan secara berlebihan pada sel teka dan sel granulosa, sedangkan reseptor follicle stimulating hormone mengalami represi ekspresi gen pada sel granulosa. Hiperandrogenisme yang dimediasi reseptor androgen juga diatur secara berbeda pada Sindrom Ovarium Polikistik.
Gambaran evaluasi fungsi ginjal (kadar ureum, kreatinin dan asam urat darah) pasca covid-19 di wilayah gang anwar tahun 2022 Jannah, Insani Fitrahulil; Sari, Mike Permata; Radhina, Afifa
Indonesian Journal of Health Science Vol 4 No 6 (2024)
Publisher : PT WIM Solusi Prima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54957/ijhs.v4i6.1099

Abstract

Coronavirus Disease 2019 (COVID-19) is an acute respiratory syndrome caused by Coronavirus 2 (SARS-CoV-2), which is associated with several diseases, one of which is kidney disease due to the overactivation of the pro-inflammatory ACE/ANGII/AT1R pathway. This results in the overexpression of the ACE2 pathway, leading to an increased binding site for SARS-CoV-2, where the viral antigen can accumulate in the renal tubules and cause acute kidney injury. This research The aims to examine the evaluation of kidney function by measuring blood urea, creatinine, and uric acid levels after the PPKM process to observe any trends in the decline of kidney function among the respondents. Descriptive qualitative research method with a cross-sectional approach. The research subjects are 30 residents of Anwar Alley aged 40-70 years, using purposive sampling conducted in June-July 2022. The research results showed that the largest number of respondents were female, totaling 26 (86.7%), while male respondents numbered 4 (13.3%). The most common age category was 40-49 years, with 18 respondents. The average urea level is 19.19 mg/dl, creatinine is 1.03 mg/dl, and uric acid level is 6.13 mg/dl. There are 2 individuals with high urea levels, 6 individuals with high creatinine levels, and 14 individuals with high uric acid levels. The SARS-CoV-2 antigen can accumulate in the kidneys, potentially causing acute kidney injury, which is characterized by the presence of hematuria, proteinuria, and increased levels of blood urea and creatinine. Penyakit Coronavirus 2019 (COVID-19) adalah suatu sindrom pernafasan akut yang disebabkan oleh Coronavirus 2 (SARS-CoV-2) yang berasosiasi dengan beberapa penyakit salah satunya adalah penyakit ginjal karena adanya overaktivasi Pro immflamantory ACE/ANGII/AT1R yang mengakibatkan overekspresi jalur ACE2 yang menyebabkan peningkatan bindingsite dari SARS-CoV-2 dimana antigen virus bisa terakumulasi di tubulus ginjal dan menyebabkan cidera ginjal akut. Penelitian ini untuk melihat gambaran evaluasi fungsi ginjal dengan pengukuran nilai ureum, kreatinin dan asam urat darah setelah proses PPKM untuk melihat  adanya kecendurungan penurunan fungsi ginjal responden. Metode Penelitian kualitatif deskriptif dengan pendekatan Cross sectional. Subjek penelitian adalah 30 warga gang Anwar usia 40-70 tahun dengan teknik Purposive sampling ada bulan Juni-Juli 2022. Hasil Penelitian didapatkan jumlah responden terbnyak adalah perempuan sebesar 26(86.7%) dan laki-laki sebanyak 4(13,3%) dengan kategori usia terbanyak adalah usia 40-49 tahun sebanyak 18 responden. Rerata kadar ureum adalah 19,19  mg/dl, kreatinin sebesar 1,03  mg/dl dan kadar asam urat sebesar 6,13 mg/dl. Adapun jumlah kadar ureum responden yang tinggi terdapat 2 orang, kreatinin sebanyak 6 orang dan kadar asam urat yang tinggi  sebanyak 14 orang.  Antigen SARS-Cov-2 akan terakumulasi di ginjal yang bisa menyebakan cidera ginjal akut yang ditandai dengan adanya hematuria, proteinuria serta peningkatan nilai ureum dan kreatinin darah.
The Use of Purple Eggplant Fruit Calyx Stem (Solanum melongena L.) as an Alternative Source of Lactase-Producing Lactic Acid Bacteria Mike Permata Sari; Radhina, Afifa; Indriani, Narita
Biotropika: Journal of Tropical Biology Vol. 12 No. 2 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.02.05

Abstract

Lactose intolerance is a condition where the body cannot digest lactose due to a deficiency of the lactase enzyme in the digestive system, leading to various health issues and indirectly increasing the risk of stunting. To prevent lactose intolerance, supplements containing the lactase enzyme or foods and drinks low in lactose, such as yoghurt, are recommended. Yoghurt production requires microorganisms capable of synthesizing the lactase enzyme, such as lactic acid bacteria (LAB). This research is an experimental study exploring the potential of purple eggplant stalks and petals (Solanum melongena L.) as a new source of LAB capable of synthesizing the lactase enzyme. The experiment began with making yoghurt from milk with added purple eggplant calyx stems as initial proof of LAB presence. Milk was chosen as the initial growth medium because it contains lactose, which acts as a substrate for the lactase enzyme found in LAB. The obtained yoghurt was then inoculated into selective media, resulting in the growth of gram-positive bacilli colonies. The lactase enzyme was isolated from LAB using the sonication method. Optimum enzyme activity was achieved at 70°C and pH 7, measuring 0.0061 U/Mg. This research concludes that purple eggplant stalks and petals contain LAB capable of synthesizing the lactase enzyme. Hopefully, this research will progress to purifying the lactase enzyme and developing lactase enzyme supplements, which can be tested as a therapy for lactose intolerance.