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Analisis Kadar Mirisetin Pada Ekstrak Daun Myrica Javanica Reinw. Ex Bl. Dengan Metode HPLC: Quantitative Analysis Of Myricetin In Extract Leaf Myrica Javanica Reinw. Ex Bl. Using HPLC Arianti, Varda; Arianto, Steven; Maulina, Devi; Adiana, Sylvi
Binawan Student Journal Vol. 5 No. 3 (2023)
Publisher : Direktorat Penelitian, Pengabdian Masyarakat, Dan Kerjasama Universitas Binawan (DPPMK Universitas Binawan)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54771/bsj.v5i3.1355

Tanaman Myrica javanica mengandung senyawa mirisetin yang berkhasiat sebagai: antiinflamasi, analgesik, antitumor, hepatoprotektor, antidiabetes dan antibakteri. Metode yang digunakan pada penelitian ini High Performance Liquid Chromatography (HPLC). Pengujian ini dilengkapi dengan validasi metode pengujian, yaitu Linearitas, Akurasi, Presisi, LOD dan LOQ. Hasil penelitian ini didapatkan pengujian linearitas adalah nilai r2 yaitu 0.9981 (r = 0.999). Persen (%) akurasi adalah 100,53%. Nilai presisi yaitu 0,98%. Nilai LOD diperoleh sebesar 1,93 dan nilai LOQ sebesar 6,43. Hasil uji kadar mirisetin pada yang diperoleh pada konsentrasi ekstrak 5000 ppm adalah sebesar 6,104.

Pemeriksaan Skrining Alternatif PAP SMEAR Parwati, Dewi; Arianto, Steven; Pannyiwi, Rahmat; Rahmat, Rezqiqah Aulia; Sabriana, Riska; Rosida, Rosida
Sahabat Sosial: Jurnal Pengabdian Masyarakat Vol. 1 No. 4 (2023): Sahabat Sosial: Jurnal Pengabdian Masyarakat (September)
Publisher : Asosiasi Guru dan Dosen Seluruh Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59585/sosisabdimas.v1i4.169

The incidence of cervical cancer in Indonesia reached 20,928 and cervical cancer is in second place after breast cancer. Pap smear is a microscopic examination of cells obtained from a cervical smear to detect early changes or abnormalities in the cervix before the cells become cancer and can be carried out by health workers other than gynecologists. The purpose of a pap smear is: a). Try to find cells that are not normal and can develop into cervical cancer. b). A tool to detect pre-cervical cancer symptoms for someone who has not yet suffered from cancer. c). Know the abnormalities that occur in cervical cancer cells. d). To determine the level of malignancy of the cancer cells, the examination is carried out by looking at the cervix which has been treated with 3-5% acetic acid inspectively. The methods used with the descriptive approach include: Conducting counseling about early detection of cervical cancer, Carrying out questions and answers about cervical cancer and the IVA test, Carrying out initial screening, namely no menstruation during the IVA test, no intercourse for 2 days, Carrying out an anamnesis on each - each respondent. Carrying out IVA tests in communities that have met the requirements for IVA tests. The number of respondents who met the requirements for the IVA test was 86 respondents. This IVA test was carried out for 3 days in three different locations, then the results of 46 people were obtained with positive IVA results. Conclusion: It is hoped that the IVA test carried out on the target group that has been held will be able to raise the target group's awareness of the importance of reproductive health.

Epigenetik Pada Sindrom Ovarium Polikistik: Gangguan Proses Folikulogenesis Yang Melibatkan Gen Reseptor Androgen, Reseptor Follicle-Stimulating Hormone Dan Reseptor Luteinizing Hormone Arianto, Steven; Radhina, Afifa; Shari, Amalia; Jannah, Insani Fitrahulil; Sari, Mike Permata
Indonesian Journal of Health Science Vol 4 No 1 (2024)
Publisher : PT WIM Solusi Prima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54957/ijhs.v4i1.683

Sindrom Ovarium Polikistik (SOPK) merupakan salah satu gangguan hormonal yang dialami oleh wanita. Gangguan ini menyebabkan disregulasi proses neuroendokrin dan mengganggu proses pembentukan folikel (folikulogenesis). Etiologi gangguan folikulogenesis pada Sindrom Ovarium Polikistik disebabkan oleh reseptor androgen, reseptor follicle stimulating hormone (FSH), dan reseptor luteinizing hormone (LH). Beberapa pendekatan ilmiah digunakan untuk memahami penyebab gangguan folikulogenesis pada Sindrom Ovarium Polikistik, salah satunya epigenetik. Faktor epigenetik seperti metilasi DNA dan modifikasi histon diduga menjadi salah satu penyebab gangguan pengaturan sinyal luteinizing hormone dan follicle stimulating hormone pada folikulogenesis. Sinyal luteinizing hormone dan follicle-stimulating hormone diatur oleh reseptor luteinizing hormone dan reseptor follicle stimulating hormone yang memiliki ekspresi messenger RNA (mRNA) yang berbeda dibandingkan dengan kondisi normal. Reseptor luteinizing hormone diekspresikan secara berlebihan pada sel teka dan sel granulosa, sedangkan reseptor follicle stimulating hormone mengalami represi ekspresi gen pada sel granulosa. Hiperandrogenisme yang dimediasi reseptor androgen juga diatur secara berbeda pada Sindrom Ovarium Polikistik.

Viroinformatic Study of Star Anise (Illicium verum) as Mpro Inhibitor in SARS-CoV-2 Infection Arianto, Steven; Lakoan, Milda Rianty; Arianti, Varda
Nusantara Science and Technology Proceedings The 1st International Conference of Health Institut Kesehatan Mitra Bunda 2024
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4304

The spread and infection of SARS-CoV-2 towards the end of 2019 started the COVID-19 outbreak. Additionally, the antiviral medications that are still missing. Spices were among the many traditional medicines used in Indonesia. This research aims to find possible bioactive compounds in star anise with antiviral qualities to treat COVID-19 against SARS-CoV-2 proteases through the in-silico approach. Samples were obtained from the Protein Data Bank (PDB) and PubChem (NCBI, USA). Then, drug-likeness analysis was performed on the SCFBIO web server using the Lipinski rule of five. Additionally, the binding activity and molecular interaction by PoseView web server and PyMol software v2.4.1 (Schrödinger, Inc., USA) were determined by the blind docking approach using PyRx 0.8 software. Using Lipinski's rule of five, the eighty-one bioactive chemicals in star anise were examined to see how similar they were to therapeutic compounds. The molecular docking procedure was then executed using PyMOL and PyRx 0.8 (Virtual Screening Tool) and its interaction with Mpro protein. Methylphenyl has been revealed to have the lowest binding energy for Mpro SARS-CoV-2 and great potential as an inhibitor of SARS-CoV-2 viral replication based on the comprehensive research conducted on these bioactive compounds. However, since the results are only computational, a wet lab study is required for validation. Of 38 drug-like bioactive chemicals of star anise, only methylphenyl has the lowest binding affinity, which can bind and function as inhibitor of the Mpro protease enzyme. A can obstruct the SARS-CoV-2 virus's reproduction. As a result, the SARS-CoV-2 virus cannot assemble its genetic material, capsids, and other body components. Viral replication can be stopped to lower the amount of genetic material copied from the virus.

PENINGKATAN PERILAKU HIDUP BERSIH DAN SEHAT PADA ANAK DI TAMAN BACA AMALIA TANGERANG SELATAN Setiawan, Doni; Siti Nuraeni, Hanny; Alina Putri Sugianto, Fitri; Hadi Sulhan, Muhammad; Arianto, Steven; Fadilah, Apriyanah; Septiani, Septiani; Noviandani, Shifa; Abil Fadli, Malik; Fransiskus, Parto
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 7, No 8 (2024): MARTABE : JURNAL PENGABDIAN MASYARAKAT
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v7i8.2953-2957

Perilaku Hidup Bersih dan Sehat (PHBS) serta Cuci Tangan Pakai Sabun (CTPS) belum berhasil secara optimal karena berbagai faktor dan hambatan. Oleh karena itu, pentingnya PHBS dimasukkan ke dalam visi misi Indonesia sehat 2010. Selain itu, PHBS juga termasuk dalam rencana strategis Kementerian Kesehatan 2010-2014 dan mulai disosialisasikan melalui berbagai kegiatan di desa/kelurahan siaga aktif. Kegiatan ini bertujuan untuk membangkitkan perilaku hidup bersih dan sehat di kalangan anak-anak pemulung berusia dini serta masyarakat sekitar. Metode pengabdian kepada masyarakat dilaksanakan dengan memberikan edukasi dan bimbingan. Materi penyuluhan meliputi PHBS dan CTPS. Selama tiga sesi kegiatan, materi disampaikan menggunakan metode ceramah, video animasi, dan praktik langsung yang disesuaikan dengan kebutuhan anak-anak usia dini. Data hasil kegiatan pengabdian kepada masyarakat sebelum dilakukan pengabdian kepada masyarakat menunjukkan bahwa anak-anak sekitar Taman Baca Amalia cenderung kurang mempraktikkan PHBS dan CTPS dengan tingkat pemahaman rata-rata sebesar 46,3%. Evaluasi tingkat pemahaman anak-anak setelah kegiatan pengabdian pada masyarakat menunjukkan bahwa rata-rata pemahaman mencapai 98,54%. Hal ini menandakan bahwa anak-anak telah memahami dengan baik materi PHBS dan CTPS yang disampaikan. Pengabdian kepada masyarakat berhasil meningkatkan kesadaran dan pemahaman anak-anak tentang pentingnya perilaku hidup bersih dan sehat serta cara mencuci tangan dengan benar.

In silico approach of antiviral compound from Cymbopongon citratus as the main protease SARS-CoV-2 inhibitor Arianto, Steven
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.5754

A pandemic known as COVID-19 is brought on by the RNA virus SARS-CoV-2. SARS-CoV-2 has some protease enzymes, such as main proteases (Mpro), that regulate the expression of viral non-structural proteins. The Mpro protein is a possible target for SARS-CoV-2 antiviral therapy because the inhibition of Mpro will disrupt the virus replication process and stop the virus life cycle. Lemongrass is an Indonesian spice with bioactive compounds whose benefits have not been widely explored, especially as an antiviral for SARS-CoV-2. This study aimed to identify potential bioactive chemicals in lemongrass that have antiviral properties for treating COVID-19. This methodology research began by preparing the bioactive compounds of lemongrass and Mpro protein. Using Lipinski’s rule of five, the nine bioactive chemicals found in lemongrass were examined to see how similar they were as therapeutic compounds. Next, PyRx 0.8 (Virtual Screening Tool) and PyMOL were used to perform molecular docking and its interaction with the Mpro protein. Diethyl phthalate has antiviral and anti-inflammatory activity with low binding energy (-5.8 kcal/mol) for Mpro and is stably bound (RMSF < 3Å) via hydrophobic bonds (His41, Met165) and hydrogen bonds (Phe140, His163, His164, Glu166, Gln189). It is concluded that diethyl phthalate on lemongrass demonstrated high promise as an inhibitor of SARS-CoV-2 viral replication based on the findings of the comprehensive investigation of these bioactive chemicals.

Kardiomiopati peripartum vs Kardiomiopati peripartum dengan preeklamsia: dari pendekatan biomolekuler hingga manajemen Fahreza, Lufthi; Winato, Brigad Mahardika; Nugrahani, Annisa Dewi; Arianto, Steven
Jurnal Biomedika dan Kesehatan Vol 7 No 3 (2024)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2024.v7.351-361

Peripartum cardiomyopathy (PPCM) is a potentially life-threatening pregnancy-related condition that typically occurs during the peripartum period. It is characterized by impaired function of the left ventricle and heart failure. Preeclampsia is recognized as a risk factor for PPCM and can coexist with this condition. However, there is limited available data that explains the variations in treatment approaches between cases of peripartum cardiomyopathy with and without preeclampsia. Hence, the objective of this study is to examine the distinctions between PPCM and PPCM accompanied by preeclampsia, encompassing their molecular, pathogenic, and pathophysiological aspects, as well as their treatment and management strategies. These factors could potentially play a role in influencing the treatment of patients diagnosed with peripartum cardiomyopathy. A thorough review of existing literature was conducted between June and August 2023. Articles were retrieved from medical databases including PubMed, EBSCOhost, Medline, SAGE, JSTOR, and ScienceDirect, utilizing keywords such as "Peripartum Cardiomyopathy," "PPCM," "Preeclampsia," "Biomolecular," and "Treatment." This study encompassed original articles written in either English or Indonesian, focusing on a ten-year time frame. Articles categorized as literature reviews, systematic reviews, and/or meta-analyses were excluded from consideration in this study. The Results of this review, In instances of PPCM accompanied by preeclampsia, the secretion of sFlt1 from the placenta is notably elevated compared to cases of PPCM alone. PPCM and PPCM with preeclampsia exhibit distinct pathogenic and pathophysiological characteristics. Patients with concentric left ventricular remodeling (PPCM with preeclampsia) may not derive the same degree of benefit from angiotensin-converting enzyme inhibitors, angiotensin receptor blockers, or beta-blockers as those with eccentric left ventricular remodeling (PPCM without preeclampsia). The Conclusion of this review, PPCM and PPCM with preeclampsia are differentiated by underlying biomolecular factors, pathogenesis, and pathophysiology. Furthermore, they respond differently to treatment and have different approaches to management.

PENINGKATAN PERILAKU HIDUP BERSIH DAN SEHAT PADA ANAK DI TAMAN BACA AMALIA TANGERANG SELATAN Setiawan, Doni; Siti Nuraeni, Hanny; Alina Putri Sugianto, Fitri; Hadi Sulhan, Muhammad; Arianto, Steven; Fadilah, Apriyanah; Septiani, Septiani; Noviandani, Shifa; Abil Fadli, Malik; Fransiskus, Parto
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 7, No 8 (2024): MARTABE : JURNAL PENGABDIAN MASYARAKAT
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v7i8.2953-2957

Perilaku Hidup Bersih dan Sehat (PHBS) serta Cuci Tangan Pakai Sabun (CTPS) belum berhasil secara optimal karena berbagai faktor dan hambatan. Oleh karena itu, pentingnya PHBS dimasukkan ke dalam visi misi Indonesia sehat 2010. Selain itu, PHBS juga termasuk dalam rencana strategis Kementerian Kesehatan 2010-2014 dan mulai disosialisasikan melalui berbagai kegiatan di desa/kelurahan siaga aktif. Kegiatan ini bertujuan untuk membangkitkan perilaku hidup bersih dan sehat di kalangan anak-anak pemulung berusia dini serta masyarakat sekitar. Metode pengabdian kepada masyarakat dilaksanakan dengan memberikan edukasi dan bimbingan. Materi penyuluhan meliputi PHBS dan CTPS. Selama tiga sesi kegiatan, materi disampaikan menggunakan metode ceramah, video animasi, dan praktik langsung yang disesuaikan dengan kebutuhan anak-anak usia dini. Data hasil kegiatan pengabdian kepada masyarakat sebelum dilakukan pengabdian kepada masyarakat menunjukkan bahwa anak-anak sekitar Taman Baca Amalia cenderung kurang mempraktikkan PHBS dan CTPS dengan tingkat pemahaman rata-rata sebesar 46,3%. Evaluasi tingkat pemahaman anak-anak setelah kegiatan pengabdian pada masyarakat menunjukkan bahwa rata-rata pemahaman mencapai 98,54%. Hal ini menandakan bahwa anak-anak telah memahami dengan baik materi PHBS dan CTPS yang disampaikan. Pengabdian kepada masyarakat berhasil meningkatkan kesadaran dan pemahaman anak-anak tentang pentingnya perilaku hidup bersih dan sehat serta cara mencuci tangan dengan benar.

Histoplasmosis: Etiologi, patofisiologi, morfologi, diagnosis, dan pengobatan Jannah, Insani Fitrahulil; Arianto, Steven
Indonesian Journal of Health Science Vol 4 No 6 (2024)
Publisher : PT WIM Solusi Prima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54957/ijhs.v4i6.1110

Histoplasmosis is a fungal infection caused by Histoplasma capsulatum, commonly found in contaminated soil, particularly in endemic areas. This disease is of concern as it can be potentially fatal for individuals with weakened immune systems. This study aims to summarize and analyze current information regarding the etiology, pathophysiology, diagnosis, and treatment of histoplasmosis. The research method employed is a literature review, collecting and analyzing relevant articles from various reputable sources.The findings indicate that diagnosing histoplasmosis is often challenging with conventional methods, such as microscopy and culture, which have limitations in sensitivity and specificity. In contrast, molecular methods like PCR demonstrate better results in detecting infections, especially in difficult cases. The conclusion of this study emphasizes the importance of a comprehensive understanding of histoplasmosis and the need for developing more accurate diagnostic methods to enhance the detection and management of this disease. Histoplasmosis adalah infeksi jamur yang disebabkan oleh Histoplasma capsulatum, yang sering ditemukan di tanah yang terkontaminasi, terutama di daerah endemik. Penyakit ini menjadi perhatian karena dapat berpotensi fatal pada individu dengan sistem imun yang lemah. Penelitian ini bertujuan untuk merangkum dan menganalisis informasi terkini mengenai etiologi, patofisiologi, diagnosis, dan pengobatan histoplasmosis. Metode yang digunakan dalam penelitian ini adalah tinjauan literatur, dengan mengumpulkan dan menganalisis artikel- artikel relevan dari berbagai sumber terpercaya. Hasil penelitian menunjukkan bahwa diagnosis histoplasmosis sering kali sulit dilakukan dengan metode konvensional, seperti mikroskopis dan kultur, yang memiliki keterbatasan dalam sensitivitas dan spesifisitas. Sebaliknya, metode molekuler seperti PCR menunjukkan hasil yang lebih baik dalam mendeteksi infeksi, terutama pada kasus yang sulit. Kesimpulan dari penelitian ini menekankan pentingnya pemahaman yang mendalam tentang histoplasmosis dan perlunya pengembangan metode diagnostik yang lebih akurat untuk meningkatkan deteksi dan penanganan penyakit ini.

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