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All Journal Jurnal Analis Kesehatan Mukhtabar: Journal of Medical Laboratory Technology Jurnal Pengabdian Masyarakat Kesehatan Indonesia
Juliastuti, Aditya
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Efektivitas Kuersetin Fraksinasi Daun Teh Hijau Sebagai Antioksidan dan Antiagregasi Platelet Terhadap Stabilitas Bahan Kontrol dan Darah Simpan Hayati, Eem; Durachim, Adang; Nurhayati, Betty; Juliastuti, Aditya
Jurnal Analis Kesehatan Vol. 10 No. 2 (2021): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v10i2.2688

Abstract

Fungsi Puskesmas berorientasi kepada upaya kuratif dan rehabilitatif, saat ini bergeser kepada upaya preventif dan promotif tanpa mengabaikan orientasi sebelumnya. Untuk dapat melaksanakan fungsi pelayanan laboratorium di puskesmas dibutuhkan sumber daya manusia yang mencukupi baik jumlah maupun mutunya. Berdasarkan Permenkes Nomor 46 Tahun 2015 tentang Akreditasi Puskesmas dokumen yang harus terpenuhi sesuai kriteria persyaratan akreditasi yaitu terdapatnya SOP tentang Pemantapan Mutu Internal (PMI) dan Pemantapan Mutu Eksternal (PME).  Bahan kontrol komersial sangat baik digunakan untuk pelaksanaan PMI karena sangat stabil sampai masa expiry date nya, namun secara ekonomis relatif mahal sehingga  untuk penggunaan bahan kontrol komersial secara rutin di laboratorium terutama laboratorium puskesmas atau laboratorium institusi pendidikan kurang terjangkau dan cukup memberatkan. Bahan kontrol yang banyak digunakan di antaranya bahan kontrol darah lengkap komersial untuk  pemeriksaan hematologi.  Berdasarkan hal tersebut tujuan penelitian adalah untuk menganalisis pembuatan bahan kontrol buatan dari Packed Red Cell sebagai alternatif penggunaan bahan kontrol komersial. Bahan tambahan yang akan digunakan  dalam penelitian adalah kuersetin yang diperoleh dari hasil fraksinasi daun teh hijau. Berdasarkan penelitian sebelumnya telah diketahui kuersetin dapat berfungsi sebagai antioksidan dan anti agregasi platelet. Metode penelitian yang digunakan adalah eksperimen laboratorium. Hasil penelitian setelah diuji secara statistik dengan uji GLM dapat disimpulkan bahan kontrol yang ditambah dengan kuersetin, jumlah eritrosit stabil selama 20 hari, lekosit stabil 15 hari dan trombosit disimpan selama 5 hari sudah tidak stabil, sedangkan bahan kontrol komersil disimpan selama 15 hari sudah terjadi penurunan jumlah sel.
UPAYA PREVENTIF PENYEBARAN TUBERKULOSIS MELALUI PEMBERDAYAAN MASYARAKAT DALAM MENGOLAH DAUN KELOR SEBAGAI SPRAY DESINFEKTAN DI RT 02 RW 02 KELURAHAN PASIRKALIKI, KECAMATAN CIMAHI UTARA, KOTA CIMAHI Abror, Yogi Khoirul; Merdekawati, Fusvita; Juliastuti, Aditya
Jurnal Pengabdian Masyarakat Kesehatan Indonesia Vol. 3 No. 2 (2024): Jurnal Pengabdian Masyarakat Kesehatan Indonesia
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jpmki.v3i2.2619

Abstract

Tuberculosis (TB) remains a global health issue, with Indonesia ranking second worldwide in TB cases. The high incidence of TB is partly due to its ease of transmission via droplets in densely populated areas. One preventive measure is improving environmental hygiene through disinfectants. However, chemical-based disinfectants often leave residues, prompting the need for natural alternatives, such as Moringa oleifera leaves, which exhibit antibacterial potential. This community service activity aimed to enhance the knowledge and skills of Posyandu Mawar 01 and 02 cadres in RT 02 RW 02, Pasirkaliki Village, Cimahi Utara Sub-district, on processing moringa leaves into disinfectant sprays to prevent TB transmission. The program included preparation, education, mentoring, pre-tests, and post-tests. Supporting materials such as presentations, leaflets, videos, and moringa-based disinfectant spray products were distributed to cadres for further education. The results showed a 42% increase in cadres' knowledge, with an average post-test score of 85 compared to a pre-test score of 60. This activity effectively improved cadres' understanding of TB prevention using natural materials. It is hoped that similar activities will be conducted regularly to raise community awareness and capacity in TB control.
Assessment of Analytical Specificity in qPCR: SYBR Green and TaqMan Probe Methods for Isonazid-Resistant Mycobacterium tuberculosis Merdekawati, Fusvita; Suryawan, Muhammad Raihan; Nurhayati, Betty; Rinaldi, Sonny Faisal; Juliastuti, Aditya
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 1 (2025): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i1.793

Abstract

Background & Objective: Mycobacterium tuberculosis, often referred to as M. tuberculosis, is an infectious pathogen that is responsible for causing tuberculosis (TB), a prevalent condition that is a leading cause of death globally. The spread of tuberculosis bacteria that are resistant to certain medicines, such as isoniazid, is currently on the rise. Therefore, molecular testing, such as quantitative polymerase chain reaction (qPCR), is needed to rapidly and reliably identify isoniazid-resistant tuberculosis germs. Method: The purpose of this study was to assess the analytical specificity of TaqMan Probe and SYBR Green qPCR techniques for the detection of isoniazid-resistant tuberculosis. This study was descriptive and quantitative. Analytical specificity was ascertained using MTB DNA with the S315G mutation spiked with E. coli DNA. A paired t-test was used to assess the primary data. Result: The results show that the analytical specificity values based on the significance of the paired t-test for the SYBR Green and TaqMan Probe methods were 0.398 and 0.790, respectively. Conclusion: Based on these results, it can be concluded that the analytical specificity of the TaqMan Probe qPCR method was 1.99 times greater than that of the SYBR Green method.
Co-Authors Abror, Yogi Khoirul Durachim, Adang Hayati, Eem Merdekawati, Fusvita Nurhayati, Betty Rinaldi, Sonny Faisal Suryawan, Muhammad Raihan