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Optimasi suhu amplifikasi DNA pada quantitative polymerase chain reaction untuk identifikasi Mycobcterium tuberculosis resistan isoniazid Endarwati, Dwi Veni; Indra, Asep Iin Nur; Hardiana, Acep Tantan; Abror, Yogi Khoirul; Nurhayati, Betty; Merdekawati, Fusvita
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.61-70

Background: Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis and is a serious threat to global health. The methods can be used to detect and identify the bacteria is quantitative polymerase chain reaction (qPCR). In this method, denaturation and extension temperatures are determining factors of success that needs to be optimized. Objective: This study aims to optimize denaturation and extension temperatures in M. tuberculosis DNA amplification. Methods: The research used quasi-experimental design. The denaturation temperature optimized were 93, 94, 95, 96, and 97°C, and the extension temperature optimized were 58, 59, 60, 61, and 62°C. The test sample was a 1 ml sputum sample isolated from a patient with isoniazid-resistant M. tuberculosis. Optimization was performed using seven test primers, namely S315T, S315N, S315I, S315R, S315G, S315L, and R463B with the katG gene target and data analysis using Ms Excel. Data optimization results were processed with Excel by taking the lowest Ct value. Results: The results showed that the optimization temperatures for denaturation were different for each primer used. Primers S315T, S315R, and S315G, optimal with denaturation temperature of 96°C, primer S315N optimal with 94°C, primers S315I and R463B optimal with 93°C, and for primer S315L optimal with 95°C, with the most widely used temperature is 96°C. The optimal extension temperature was 58°C for primers S315T, S315N, S315I, and R463B, at 60°C for primers S315R and S315G, and at 61°C for primer S315L. Conclusion: The optimal denaturation temperature in this study was 96°C and the optimal extension temperature was 58°C.

ANALISIS GAMBARAN JUMLAH LEUKOSIT, VIRAL LOAD DAN KEPATUHAN MINUM OBAT ARV PADA ORANG DENGAN HIV (ODHIV) Setyaningsih, Ari; Abror, Yogi Khoirul
Jurnal Ilmiah Kesehatan Vol 16 No 1 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v16i1.2086

AbstrakÂ Â HIV atau Human Immunodeficiency Virus yang menginfeksi sel darah putih dapat menyebabkan turunnya kekebalan tubuh manusia. Akibat menurunnya kekebalan tubuh maka orang tersebut sangat mudah terkena berbagai penyakit opurtunistik. Kepatuhan meminum obat ARV menjadi faktor utama dalam mewujudkan keberhasilan terapi ARV. Pemeriksaan Viral Load merupakan standar pemeriksaan utama untuk memantau ODHIV yang sedang menjalani terapi ARV. Pemeriksaan leukosit menjadi salah satu pemeriksaan dasar yang dilakukan untuk melihat ada tidaknya infeksi oputunistik pada ODHIV. Tujuan Penelitian untuk mengetahui gambaran jumlah leukosit, viral load dan kepatuhan minum obat ARV. Metode penelitian adalah kuantitatif denganÂ jenis penelitian deskriftif analisis. Sampel penelitian berjumlah 30 ODHIV. Hasil pemeriksaan leukosit yang mengalami penurunan sebanyak 3 sampel yaitu 10%. Jumlah leukosit yang berada di nilai normal sebanyak 27 sampel yaitu 90%.Hasil data nilai viral load yang terdeteksi 18 sampel 60%, nilai viral load yang tidak terdeteksi 12 sampel 40%. Sampel yang tersupresi 29 orang 96,7% dan yang tidak tersupresi 1 3,3% orang. Jumlah ODHIV yang memiliki kepatuhan tinggi minum obat ARV sebanyak 24 orang yaitu 80%, yang memiliki kepatuhan sedang 5 orang 16,7% dan tingkat kepatuhan rendah 1 orang 3,3%. Keberhasilan virologis didapatkan pada 23 ODHIV dimana jumlah leukosit berada pada nilai normal, jumlah viral load tersupresi dan memiliki tingkat kepatuhan tinggi, 1 ODHIV mengalami penurunan jumlah leukosit, viral load tidak tersupresi dan memiliki tingkat kepatuhan rendah, 1 ODHIV berada pada keadaan kegagalan virologis dimana nilai viral load >400 copies/ML darah dan 5 ODHIV memiliki tingkat kepatuhan sedang sehingga nilai viral load masih terdeteksi.Â Kata kunci : ODHIV, Kepatuhan Minum Obat, Viral LoadÂ Â Â Â Â Â Â Â Â Â Abstract Â Â HIV or Human Immunodeficiency Virus, which infects white blood cells, can cause a decline in the human body's immunity. Due to the weakened immune system, individuals are highly susceptible to various opportunistic diseases. Adherence to ARV medication is a key factor in achieving successful ARV therapy. Viral Load testing is the primary standard examination for monitoring individuals with HIV undergoing ARV therapy. Leukocyte examination is one of the fundamental tests conducted to determine the presence of opportunistic infections in HIV patients. The research aim is to understand the leukocyte count, viral load, and adherence to ARV medication. The research method employed is quantitative, utilizing descriptive analytical research. The study sample consists of 30 individuals with HIV. The results show a decrease in leukocyte count in 3 samples, accounting for 10%. 27 samples, constituting 90%, exhibited leukocyte counts within the normal range. Regarding viral load data, 18 samples detected the virus, comprising 60%, while 12 samples, totaling 40%, did not detect the virus. 29 individuals, or 96.7% of the sample, achieved viral load suppression, while 1 person, or 3.3%, did not achieve suppression. Among the individuals with HIV, 24 individuals, or 80%, exhibited high adherence to ARV medication, 5 individuals, or 16.7%, showed moderate adherence, and 1 person, or 3.3%, displayed low adherence. Virological success was observed in 23 individuals with HIV, where leukocyte counts were within the normal range, viral load was suppressed, and they exhibited high adherence. One individual showed decreased leukocyte count, unsuppressed viral load, and low adherence, while another individual experienced virological failure, with a viral load >400 copies/ML of blood, and 5 individuals exhibited moderate adherence, leading to detectable viral loads."Â Keywords: ODHIV, medication adherence, Viral Load

Analysis of Purity and Concentration Escherichia coli DNA by Boiling Method Isolation with Addition of Proteinase-K and RNase Lesiani, Bunga Rossa; Abror, Yogi Khoirul; Merdekawati, Fusvita; Djuminar, Ai
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.4773

Escherichia coli is a leading cause of Urinary Tract Infections (UTIs) in Indonesia, with approximately 180,000 cases reported annually. The more cases of UTIs, the more PCR diagnosis is needed with an accurate, fast, simple, and economical DNA isolation method. However, currently, there is no DNA purification stage from protein and RNA contaminants in the boiling DNA isolation method. This study aimed to investigate the impact of incorporating Proteinase-K and RNase into the boiling DNA isolation method on the purity and concentration of E. coli’s DNA during isolation. The boiling method involved heating to 95°C – 100°C bring to cell lysis and release of cellular components, including DNA. Urine samples were artificially contaminated with E. coli at different McFarland standards (0.25, 0.5, and 1). The boiling DNA isolation method was then performed and then analyzed for purity and concentration using a NanoDrop spectrophotometer. This study demonstrated a positive correlation between Proteinase-K and RNase concentrations used in the boiling DNA isolation method and the subsequent increase in DNA purity and concentration. An increase in DNA purity and concentration was obtained even though it was not statistically significant compared to that without Proteinase-K and RNase addition, with p-values of 0.245 for DNA purity and 0.353 for DNA concentration. Further research is recommended with higher Proteinase-K and RNase concentrations in the boiling DNA isolation method to achieve improved purity and concentration of E. coli DNA. Such enhancements could improve PCR amplification and help diagnose E. coli-related UTIs.

KORELASI KADAR TRIGLISERIDA DENGAN USIA PADA WANITA MENOPAUSE Rahayu, Ira Gustira; Abror, Yogi Khoirul
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 16 No 2 (2024): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v16i2.2398

Menopause dikenal sebagai masa berakhirnya siklus menstruasi pada wanita secara alamiah dan secara permanen saat wanita tidak lagi mengalami menstruasi selama satu tahun penuh yang diiringi dengan penurunan kadar hormon estrogen dan hormon progesteron. Gejala klinis saat menopause baik fisik, psikologis, maupun seksual akan dialami oleh wanita yang memasuki periode menopause. Hormon estrogen dianggap sebagai proteksi terjadinya dislipidemia. Kadar trigliserida cenderung meningkat dengan keadaan dimana seorang wanita sudah mulai memasuki periode menopause. Tujuan penelitian ini adalah untuk mengetahui gambaran kadar trigliserida pada pasien wanita menopause. Jenis penelitian ini adalah quasi experiment dengan desain penelitian menganalisis hasil kadar trigliserida pada wanita menopause. Populasi dari penelitian ini adalah seluruh wanita menopause yang memeriksakan kadar trigliserida di laboratorium klinik Diacare. Metode pengambilan sampel yang dilakukan adalah accidental sampling. Jumlah sampel yang diteliti adalah sebanyak 30 orang. Teknik pengumpulan data dengan menggunakan kuesioner menopause rating scale untuk mengetahui gejala klinis menopause dan juga kuesioner untuk mengetahui pola makan responden serta data kadar trigliserida responden. Hasil Uji korelasi Spearman’s didapatkan nilai p = 0,000, sehingga dapat diketahui bahwa terdapat korelasi antara kadar Trigliserida dengan usia pada wanita menopause di Laboratorium Klinik Diacare Jakarta Selatan. Hasil penelitian didapatkan berdasarkan periode menopause bahwa kadar trigliserida meningkat pada responden yang memasuki masa menopause, responden yang memasuki masa menopause yaitu sebanyak 78,6% mengalami peningkatan kadar trigliserida. Peneliti merekomendasikan pada wanita usia menopause untuk memantau kadar trigliserida secara berkala.

UPAYA PREVENTIF PENYEBARAN TUBERKULOSIS MELALUI PEMBERDAYAAN MASYARAKAT DALAM MENGOLAH DAUN KELOR SEBAGAI SPRAY DESINFEKTAN DI RT 02 RW 02 KELURAHAN PASIRKALIKI, KECAMATAN CIMAHI UTARA, KOTA CIMAHI Abror, Yogi Khoirul; Merdekawati, Fusvita; Juliastuti, Aditya
Jurnal Pengabdian Masyarakat Kesehatan Indonesia Vol. 3 No. 2 (2024): Jurnal Pengabdian Masyarakat Kesehatan Indonesia
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jpmki.v3i2.2619

Tuberculosis (TB) remains a global health issue, with Indonesia ranking second worldwide in TB cases. The high incidence of TB is partly due to its ease of transmission via droplets in densely populated areas. One preventive measure is improving environmental hygiene through disinfectants. However, chemical-based disinfectants often leave residues, prompting the need for natural alternatives, such as Moringa oleifera leaves, which exhibit antibacterial potential. This community service activity aimed to enhance the knowledge and skills of Posyandu Mawar 01 and 02 cadres in RT 02 RW 02, Pasirkaliki Village, Cimahi Utara Sub-district, on processing moringa leaves into disinfectant sprays to prevent TB transmission. The program included preparation, education, mentoring, pre-tests, and post-tests. Supporting materials such as presentations, leaflets, videos, and moringa-based disinfectant spray products were distributed to cadres for further education. The results showed a 42% increase in cadres' knowledge, with an average post-test score of 85 compared to a pre-test score of 60. This activity effectively improved cadres' understanding of TB prevention using natural materials. It is hoped that similar activities will be conducted regularly to raise community awareness and capacity in TB control.

CORRELATION OF NEUTROPHIL LYMPHOCYTE RATIO (NLR) VALUES WITH C-REACTIVE PROTEIN (CRP) LEVELS IN PNEUMONIA PATIENTS Maryani, Nita; Nurhayati, Betty; Dermawan, Asep; Abror, Yogi Khoirul; Thayyiba, Amina
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 17 No 2 (2025): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v17i2.2831

Pneumonia is an acute infection of the lungs caused by microorganisms such as bacteria, viruses, or fungi, which trigger inflammation and weaken the immune system. Early detection is crucial for guiding treatment strategies and reducing the risk of complications. One indicator that can be used for the systemic inflammatory response due to pneumonia infection is the Neutrophil Lymphocyte Ratio (NLR). In addition to NLR, C-Reactive Protein (CRP) levels can also be examined to determine the level of infectious disease activity. This study aimed to assess the correlation between NLR values and CRP levels in pneumonia patients. This is a descriptive correlational study with a cross-sectional design that was conducted on 25 adult and elderly pneumonia patients with comorbidities, selected through consecutive sampling. This research was conducted at Dr. H. A. Rotinsulu Lung Hospital, with the research time being November 6 –18, 2024. The mean neutrophil percentage was 76.9%, lymphocytes 13.4%, NLR 9.12, and CRP level 83 mg/L. Spearman’s correlation test showed a significant positive correlation between NLR and CRP (r=0.415, p=0.039). These results indicate that higher NLR values are associated with increased CRP levels in pneumonia patients.

ANALISIS GAMBARAN JUMLAH LEUKOSIT, VIRAL LOAD DAN KEPATUHAN MINUM OBAT ARV PADA ORANG DENGAN HIV (ODHIV) Setyaningsih, Ari; Abror, Yogi Khoirul
Jurnal Ilmiah Kesehatan Vol 16 No 1 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v16i1.2086

AbstrakÂ Â HIV atau Human Immunodeficiency Virus yang menginfeksi sel darah putih dapat menyebabkan turunnya kekebalan tubuh manusia. Akibat menurunnya kekebalan tubuh maka orang tersebut sangat mudah terkena berbagai penyakit opurtunistik. Kepatuhan meminum obat ARV menjadi faktor utama dalam mewujudkan keberhasilan terapi ARV. Pemeriksaan Viral Load merupakan standar pemeriksaan utama untuk memantau ODHIV yang sedang menjalani terapi ARV. Pemeriksaan leukosit menjadi salah satu pemeriksaan dasar yang dilakukan untuk melihat ada tidaknya infeksi oputunistik pada ODHIV. Tujuan Penelitian untuk mengetahui gambaran jumlah leukosit, viral load dan kepatuhan minum obat ARV. Metode penelitian adalah kuantitatif denganÂ jenis penelitian deskriftif analisis. Sampel penelitian berjumlah 30 ODHIV. Hasil pemeriksaan leukosit yang mengalami penurunan sebanyak 3 sampel yaitu 10%. Jumlah leukosit yang berada di nilai normal sebanyak 27 sampel yaitu 90%.Hasil data nilai viral load yang terdeteksi 18 sampel 60%, nilai viral load yang tidak terdeteksi 12 sampel 40%. Sampel yang tersupresi 29 orang 96,7% dan yang tidak tersupresi 1 3,3% orang. Jumlah ODHIV yang memiliki kepatuhan tinggi minum obat ARV sebanyak 24 orang yaitu 80%, yang memiliki kepatuhan sedang 5 orang 16,7% dan tingkat kepatuhan rendah 1 orang 3,3%. Keberhasilan virologis didapatkan pada 23 ODHIV dimana jumlah leukosit berada pada nilai normal, jumlah viral load tersupresi dan memiliki tingkat kepatuhan tinggi, 1 ODHIV mengalami penurunan jumlah leukosit, viral load tidak tersupresi dan memiliki tingkat kepatuhan rendah, 1 ODHIV berada pada keadaan kegagalan virologis dimana nilai viral load >400 copies/ML darah dan 5 ODHIV memiliki tingkat kepatuhan sedang sehingga nilai viral load masih terdeteksi.Â Kata kunci : ODHIV, Kepatuhan Minum Obat, Viral LoadÂ Â Â Â Â Â Â Â Â Â Abstract Â Â HIV or Human Immunodeficiency Virus, which infects white blood cells, can cause a decline in the human body's immunity. Due to the weakened immune system, individuals are highly susceptible to various opportunistic diseases. Adherence to ARV medication is a key factor in achieving successful ARV therapy. Viral Load testing is the primary standard examination for monitoring individuals with HIV undergoing ARV therapy. Leukocyte examination is one of the fundamental tests conducted to determine the presence of opportunistic infections in HIV patients. The research aim is to understand the leukocyte count, viral load, and adherence to ARV medication. The research method employed is quantitative, utilizing descriptive analytical research. The study sample consists of 30 individuals with HIV. The results show a decrease in leukocyte count in 3 samples, accounting for 10%. 27 samples, constituting 90%, exhibited leukocyte counts within the normal range. Regarding viral load data, 18 samples detected the virus, comprising 60%, while 12 samples, totaling 40%, did not detect the virus. 29 individuals, or 96.7% of the sample, achieved viral load suppression, while 1 person, or 3.3%, did not achieve suppression. Among the individuals with HIV, 24 individuals, or 80%, exhibited high adherence to ARV medication, 5 individuals, or 16.7%, showed moderate adherence, and 1 person, or 3.3%, displayed low adherence. Virological success was observed in 23 individuals with HIV, where leukocyte counts were within the normal range, viral load was suppressed, and they exhibited high adherence. One individual showed decreased leukocyte count, unsuppressed viral load, and low adherence, while another individual experienced virological failure, with a viral load >400 copies/ML of blood, and 5 individuals exhibited moderate adherence, leading to detectable viral loads."Â Keywords: ODHIV, medication adherence, Viral Load

PEMBERDAYAAN MASYARAKAT UNTUK PENCEGAHAN PENYEBARAN TUBERKULOSIS DI DESA TANIMULYA KECAMATAN NGAMPRAH KABUPATEN BANDUNG BARAT DENGAN MENERAPKAN TEKNOLOGI PEMBUATAN HANDSANITIZER BERBAHAN DASAR DAUN SELEDRI (Apium graveolens L.) Marliana, Nina; Kurniati, Iis; Abror, Yogi Khoirul; Patria, Cecep
Jurnal Pengabdian Masyarakat Kesehatan Indonesia Vol. 4 No. 2 (2025): Jurnal Pengabdian Masyarakat Kesehatan Indonesia In Progress
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jpmki.v4i2.4218

TB remains a global problem. In our country, TB prevention, including thorough handwashing, is a fairly effective health protocol for preventing TB transmission. Hand sanitizer is an alcohol-based hand cleaning liquid used to reduce contaminating bacteria by up to 99.9% by using it without rinsing with water. This liquid contains various ingredients that very quickly kill bacteria on the skin of the hands. Hand sanitizer aims to remove dirt and flora that stick to our hands. Celery (A. graveolens L.) is a type of vegetable plant with a menthol and distinctive celery aroma. Usually, celery is only used as a complementary ingredient in food preparations, but it turns out that celery contains several beneficial compounds such as flavonoids, saponins, tannins, essential oils as antibacterials. Therefore, celery can be used as a hand sanitizer. The practical goal of this PPDM is for the community in Tanimulya Village, Ngamprah District, West Bandung Regency to process celery for use as a hand sanitizer. The method offered is technology transfer through counseling, training, and mentoring. The target of the PPDM program was Tani Mulya Village in West Bandung Regency, given the dense population and high pollution in the area, necessitating hand sanitizer to prevent the spread of tuberculosis through the hands. The results of the PPDM program in Tani Mulya Village showed an increase in community knowledge and skills, as evidenced by the pre- and post-test results. The average pre-test score was 66.2, while the post-test score increased to 75.7.

UPAYA PREVENTIF PENYALAHGUNAAN NARKOBA MELALUI EDUKASI, SOSIALISASI DAN PEMERIKSAAN NARKOBA PADA SISWA/I SMK TI PEMBANGUNAN KAMPUS III Merdekawati, Fusvita; Abror, Yogi Khoirul; Solihat, M. Firman; Khairunnisa, Syirin Nisrina; Rahmawati, Tia; Dzulhizza, Zaskia Puteri
Jurnal Pengabdian Masyarakat Kesehatan Indonesia Vol. 4 No. 2 (2025): Jurnal Pengabdian Masyarakat Kesehatan Indonesia In Progress
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jpmki.v4i2.4221

Drug abuse among teenagers has become a chronic issue in Indonesia, which is currently in a state of drug emergency. The misuse of narcotics, dangerous drugs, and other addictive substances can cause various negative impacts on users' lives, leading to mental and physical damage, as well as the destruction of social relationships, morals, careers, and finances. The problem of drug abuse among youth is not easy to solve, as it requires collaboration from multiple parties, including the government, police, community members, the media, families, schools, and the teenagers themselves. The main goal of this community service program was to provide students at SMK TI Pembangunan Campus III with a comprehensive understanding of narcotics and their effects on life. The program used a structured method that involved: Pre-test: To assess the students' initial understanding of drugs. Education and Socialization: To provide knowledge and awareness. Urine Screening: As a screening tool for drug use among students. Post-test: To evaluate the increase in understanding after the program. The program successfully increased students' understanding of drugs. This finding strongly suggests that the educational program was effective in achieving its goal. The improvement in understanding was reflected not only in the rise of average scores but also in the consistent improvement among almost every individual student who was measured. These positive results imply that the methods and curriculum used in the program are worth maintaining or even developing further.

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