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All Journal Communications in Science and Technology Jurnal Analis Kesehatan Jurnal Riset Kesehatan Poltekkes Depkes Bandung JMLS: Journal of Medical Laboratory and Science Current Biomedicine Mukhtabar: Journal of Medical Laboratory Technology Jurnal Kesehatan Siliwangi Jurnal Pengabdian Masyarakat Kesehatan Indonesia Poltekita: Jurnal Pengabdian Masyarakat
Nurhayati, Betty
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Optimasi suhu amplifikasi DNA pada quantitative polymerase chain reaction untuk identifikasi Mycobcterium tuberculosis resistan isoniazid Endarwati, Dwi Veni; Indra, Asep Iin Nur; Hardiana, Acep Tantan; Abror, Yogi Khoirul; Nurhayati, Betty; Merdekawati, Fusvita
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.61-70

Abstract

Background: Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis and is a serious threat to global health. The methods can be used to detect and identify the bacteria is quantitative polymerase chain reaction (qPCR). In this method, denaturation and extension temperatures are determining factors of success that needs to be optimized. Objective: This study aims to optimize denaturation and extension temperatures in M. tuberculosis DNA amplification. Methods: The research used quasi-experimental design. The denaturation temperature optimized were 93, 94, 95, 96, and 97°C, and the extension temperature optimized were 58, 59, 60, 61, and 62°C. The test sample was a 1 ml sputum sample isolated from a patient with isoniazid-resistant M. tuberculosis. Optimization was performed using seven test primers, namely S315T, S315N, S315I, S315R, S315G, S315L, and R463B with the katG gene target and data analysis using Ms Excel. Data optimization results were processed with Excel by taking the lowest Ct value. Results: The results showed that the optimization temperatures for denaturation were different for each primer used. Primers S315T, S315R, and S315G, optimal with denaturation temperature of 96°C, primer S315N optimal with 94°C, primers S315I and R463B optimal with 93°C, and for primer S315L optimal with 95°C, with the most widely used temperature is 96°C. The optimal extension temperature was 58°C for primers S315T, S315N, S315I, and R463B, at 60°C for primers S315R and S315G, and at 61°C for primer S315L. Conclusion: The optimal denaturation temperature in this study was 96°C and the optimal extension temperature was 58°C.
Konsentrasi dan kemurnian ekstraksi DNA metode sonikasi dan spin column dari sampel dahak penderita tuberkulosis Saputra, Fitrianingsih; Indra, Asep Iin Nur; Djuminar, Ai; Merdekawati, Fusvita; Nurhayati, Betty
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.84-92

Abstract

Background: The Polymerase Chain Reaction (PCR) method can identify Mycobacterium tuberculosis in a sputum sample of a patient with TB (TB). One crucial step to ensure accurate PCR results is the DNA extraction process. Objective: The research aims to compare the concentration and purity of DNA from the sputum of TB patients using ultrasound and spin column extraction techniques. Methods: The research uses descriptive study designs with post-only design strategies. The primary data was derived from 18 sputum specimens from TB patients. Concentration measurement and DNA purity testing using a nanodrop spectroscopic photometer. Results: DNA extraction by ultrasound method has an average concentration of 18.9 ± 8.5 ng/L, with a peak of 37.6 ng/ L. The spin column method produces an average of 55.5 ± 27.9 ng/μL; the peak is 105.0 ng/ μL. The purity value of the DNA extract is in the range of 1.8 ± 2.0 with the ultrasound method of 61% and the spin column of 78%. Conclusion: The sonication method has a lower average concentration and a higher percentage of purity than the spin column method, and there are differences in concentrations and purity values between the two methods.
Efektivitas Kuersetin Fraksinasi Daun Teh Hijau Sebagai Antioksidan dan Antiagregasi Platelet Terhadap Stabilitas Bahan Kontrol dan Darah Simpan Hayati, Eem; Durachim, Adang; Nurhayati, Betty; Juliastuti, Aditya
Jurnal Analis Kesehatan Vol. 10 No. 2 (2021): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v10i2.2688

Abstract

Fungsi Puskesmas berorientasi kepada upaya kuratif dan rehabilitatif, saat ini bergeser kepada upaya preventif dan promotif tanpa mengabaikan orientasi sebelumnya. Untuk dapat melaksanakan fungsi pelayanan laboratorium di puskesmas dibutuhkan sumber daya manusia yang mencukupi baik jumlah maupun mutunya. Berdasarkan Permenkes Nomor 46 Tahun 2015 tentang Akreditasi Puskesmas dokumen yang harus terpenuhi sesuai kriteria persyaratan akreditasi yaitu terdapatnya SOP tentang Pemantapan Mutu Internal (PMI) dan Pemantapan Mutu Eksternal (PME).  Bahan kontrol komersial sangat baik digunakan untuk pelaksanaan PMI karena sangat stabil sampai masa expiry date nya, namun secara ekonomis relatif mahal sehingga  untuk penggunaan bahan kontrol komersial secara rutin di laboratorium terutama laboratorium puskesmas atau laboratorium institusi pendidikan kurang terjangkau dan cukup memberatkan. Bahan kontrol yang banyak digunakan di antaranya bahan kontrol darah lengkap komersial untuk  pemeriksaan hematologi.  Berdasarkan hal tersebut tujuan penelitian adalah untuk menganalisis pembuatan bahan kontrol buatan dari Packed Red Cell sebagai alternatif penggunaan bahan kontrol komersial. Bahan tambahan yang akan digunakan  dalam penelitian adalah kuersetin yang diperoleh dari hasil fraksinasi daun teh hijau. Berdasarkan penelitian sebelumnya telah diketahui kuersetin dapat berfungsi sebagai antioksidan dan anti agregasi platelet. Metode penelitian yang digunakan adalah eksperimen laboratorium. Hasil penelitian setelah diuji secara statistik dengan uji GLM dapat disimpulkan bahan kontrol yang ditambah dengan kuersetin, jumlah eritrosit stabil selama 20 hari, lekosit stabil 15 hari dan trombosit disimpan selama 5 hari sudah tidak stabil, sedangkan bahan kontrol komersil disimpan selama 15 hari sudah terjadi penurunan jumlah sel.
PENGARUH LAMA SIMPAN DAN JENIS REAGEN COOMB’S CONTROL CELL (CCC) TERHADAP VALIDASI HASIL CROSSMATCH KOMPATIBEL Arbie, Arbie; Noviar, Ganjar; Nurhayati, Betty; Marliana, Nina
Jurnal Kesehatan Siliwangi Vol. 4 No. 3 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pemeriksaan Crossmatch atau uji silang serasi dilakukan untuk menghindari reaksi transfusi. Uji ini memastikan bahwa pasien tidak memiliki antibodi yang bereaksi terhadap antigen pada sel darah merah donor. Coomb's Control Cell (CCC) adalah suspensi sel kontrol yang dibuat dari darah golongan O Rhesus positif yang dilapisi dengan anti-D IgG. Tujuan: CCC digunakan untuk memastikan coomb’s serum atau AHG pada fase III crossmatch masih memiliki kemampuan untuk mengikat atau tidak. Metode: Penelitian ini menggunakan desain penelitian quasi eksperimen untuk menentukan pengaruh penambahan CCC terhadap derajat aglutinasi positif 2 (2+) pada validasi CCC. Derajat aglutinasi dibandingkan pada crossmatch kompatibel dengan CCC yang dibuat pada suhu 37⁰C dan suhu ruang (20 – 25⁰C) dengan waktu inkubasi 30 menit. Sampel disimpan dalam refigerator selama 0, 3, 5, dan 7 hari. Hasil: Hasil penelitian menunjukkan CCC yang inkubasi di suhu 37⁰C selama 30 menit dengan lama simpan 0, 3, 5 hari dihasilkan derajat aglutinasi+2 sedangkan dengan lama simpan 7 hari dihasilkan derajat aglutinasi +1 pada tabung mayor, minor, dan auto kontrol. CCC yang inkubasi di suhu ruang selama 30 menit dengan lama simpan 0, 3, 5 hari dihasilkan derajat aglutinasi+2 sedangkan dengan lama simpan 7 hari dihasilkan derajat aglutinasi +1 pada tabung mayor, minor, dan auto kontrol. Hasil uji Wilcoxon menunjukkan (0,046) < α = 0,05 sehingga bisa ditarik kesimpulan terdapat pengaruh lama simpan terhadap derajat aglutinasi validasi hasil crossmatch kompatibel. 
PEMBERDAYAAN MASYARAKAT MELALUI PEMANFAATAN BUAH TOMAT (Solanum lycopersium) DALAM UPAYA PENCEGAHAN RESIKO PENYUMBATAN PEMBULUH DARAH DI RW 16, KELURAHAN CIPAGERAN, KECAMATAN CIMAHI UTARA Noviar, Ganjar; Hayati, Eem; Ramadhani, Riski Nur; Nurhayati, Betty
Jurnal Pengabdian Masyarakat Kesehatan Indonesia Vol. 3 No. 1 (2024): Jurnal Pengabdian Masyarakat Kesehatan Indonesia
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jpmki.v3i1.1866

Abstract

In hypertensive patients, platelet hyperaggregation occurs. Platelet hyperaggregation will have a bad outcome in acute infarction stroke, in the form of death or neurologic deficits. One plant species that can play a role in preventing hyperaggregation complications is tomato fruit (Solanum lycopersicum) which is a horticultural plant cultivated in Indonesia. The compound content in Tomato Fruit (Solanum lycopersicum) includes solanine (0.007%), saponin, folic acid, malic acid, citric acid, bioflavonoids (including lycopene, α, and ß-carotene), protein, fat, vitamins, minerals, and histamine which function as antiaggregation. This research uses 2 methods, namely survey and sampling methods to determine the scope of empowerment and education in the form of counseling and mentoring. Apart from that, another method used is an educational method in the form of counseling and assistance to provide an understanding of the use of tomatoes to prevent the risk of blood vessel blockages. The results of the research were 20 cadres with a presentation increase of 51.67%, while the results from 20 residents had a presentation increase of 75.06%. Wilcoxon test results with p value (Sig) < 0.05. This shows that there is a significant difference in scores between the pre-test scores and the post-test scores of cadres and residents of RW. 16, Cipageran Village, North Cimahi District, Cimahi City.
Silylated-montmorillonite as co-adsorbent of chitosan composites for methylene blue dye removal in aqueous solution Saputra, Ozi Adi; Kurnia; Pujiasih, Septi; Rizki, Vanani Nur; Nurhayati, Betty; Pramono, Edi; Purnawan, Candra
Communications in Science and Technology Vol 5 No 1 (2020)
Publisher : Komunitas Ilmuwan dan Profesional Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1045.12 KB) | DOI: 10.21924/cst.5.1.2020.182

Abstract

Industrialization plays important role in the economy of developing countries, including increasing community welfare. However, the presence of poorly industries waste disposal system has negative impact to the environment. Therefore, it is necessary to overcome this problem with low-cost technology, called adsorption. In this research, silylated-montmorillonite (sMMt) has been successfully prepared as supporting material for adsorption of methylene blue by chitosan. The sMMt was characterized by FTIR, XRD, and elemental mapping techniques. The chitosan/sMMt composites were prepared through dissolution-precipitation method and produced rod-like morphology as observed by SEM. The adsorption process was carried out in a batch method by studying the pH and the adsorption contact time. The adsorption kinetic mechanism of the chitosan/sMMt nanocomposite followed pseudo-second order rather than Lagergren model indicating chemisorption predominant. The addition of silylated-montmorillonite into chitosan enhanced the methylene blue dye removal performance, which evidenced by improving Qe values by 10% compared to chitosan.
Correlation of polymerase chain reaction results with hematocrit levels and platelet counts in dengue patients in Batam City Simangunsong, Kristina; Nurhayati, Betty; Hayati, Eem; Merdekawati, Fusvita
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.1

Abstract

Background Dengue hemorrhagic fever (DHF) is a viral disease transmitted by Aedes aegypti mosquitoes, posing global public health challenge. The Riau Islands Province has the highest incidence of DHF in Indonesia. Objective This study aimed to investigate the relationship between hematocrit and platelet levels with the cycle threshold (Ct) values of polymerase chain reaction (PCR) results in DHF cases in Batam City, Riau Islands Province. Methods A descriptive correlation study was conducted using data from 102 patients infected with the dengue virus. Hematocrit and platelet counts were measured using a hematology analyzer, while Ct values for DENV1, DENV2, DENV3, and DENV4 were obtained through real-time qRT-PCR. Pearson's correlation test was employed to analyze the relationship between these variables. Results The study found no significant gender difference in DHF incidence (males: 50%, females: 50%). The highest prevalence was observed in the 6–11 years age group (44.1%), followed by the 12–18 years group (25.5%), the >18 years group (24.5%), and the 1–5 years group (11.8%). DENV3 was identified as the dominant serotype. No statistically significant correlation was found between Ct values and hematocrit (p = 0.607) or platelet counts (p = 0.323). Conclusion DHF cases in this study showed no gender disparity, with the most affected group being children aged 6–11 years, and DENV3 was the prevalent serotype. Ct values did not show a statistically significant correlation with hematocrit levels or platelet counts, suggesting that these hematological parameters may not predict viral load in DHF cases.
Pengaruh Jenis Antikoagulan dan Variasi Konsentrasi Suspensi Tes Sel ABO terhadap Hasil Pemeriksaan Serum Grouping Metode Tabung Wardhani, Azkya Zhahira; Noviar, Ganjar; Nurhayati, Betty; Hayati, Eem
Jurnal Analis Kesehatan Vol. 13 No. 2 (2024): Jurnal Analis Kesehatan
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v13i2.4574

Abstract

Pemeriksaan golongan darah adalah rangkaian yang dilakukan sebelum transfusi untuk menguji kecocokan darah pasien dan donor. Pemeriksaan golongan darah ABO mencakup cell grouping dan serum grouping dengan gold standarnya adalah metode tabung. EDTA adalah antikoagulan yang biasa digunakan, tetapi sitrat juga bisa digunakan untuk pemeriksaan golongan darah. Salah satu faktor yang bisa memengaruhi pemeriksaan serum grouping adalah konsentrasi sel uji.  Tujuan penelitian ini adalah untuk mengetahui pengaruh jenis antikoagulan K2EDTA dan Na Sitrat 3,8% dan variasi konsentrasi supensi tes sel ABO 1%, 3%, dan 5% terhadap hasil pemeriksaan serum grouping metode tabung. Jenis penelitian ini adalah eksperimen semu dengan 5 kali pengulangan. Data hasil penelitian diolah menggunakan uji Friedman dan dilanjutkan dengan uji Wilcoxon. Hasil uji Friedman terhadap pengaruh jenis antikoagulan diperoleh nilai signifikan 0,002 yang berarti terdapat pengaruh jenis antikoagulan K2EDTA dan Na Sitrat 3,8% terhadap hasil pemeriksaan serum grouping metode tabung. Hasil uji Friedman dan uji Wilcoxon terhadap variasi konsentrasi diperoleh nilai signifikan 0,002 dan 0,004 yang berarti terdapat pengaruh variasi konsentrasi suspensi tes sel ABO 1%, 3%, dan 5% terhadap hasil pemeriksaan serum grouping metode tabung. Derajat aglutinasi pada sel uji 1% dengan hasil positif 2, sedangkan 3% dan 5% pada aglutinasi positif 3 atau positif 4.
PEMBERDAYAAN MASYARAKAT MELALUI PEMANFAATAN DAUN PEPAYA DALAM UPAYA PENCEGAHAN RESIKO PENYUMBATAN PEMBULUH DARAH DI RW 16, KELURAHAN CIPAGERAN, KECAMATAN CIMAHI UTARA Hayati, Eem; Nurhayati, Betty; Noviar, Ganjar; Ihsan Nurhadi, Muhammad; Eka Setiawan, Regina; Suhartini, Entin; Nur Ramadhani, Riski
Jurnal Pengabdian Masyarakat Kesehatan Indonesia Vol. 4 No. 1 (2025): Jurnal Pengabdian Masyarakat Kesehatan Indonesia
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jpmki.v4i1.2772

Abstract

Stroke is part of Cerebro Vascular Disease (CVD), which is any form of blood circulation disorder affecting the brain or due to pathological processes in the blood vessels. Acute infarction stroke is caused by blockage or occlusion of the small cerebral arteries caused by blood clots (thrombus) originating from excessive clotting of platelets which must be given antiplatelet agents. Indonesia has biodiversity, including a diversity of plants that can be used as traditional medicine. One of the medicinal plants that contains natural ingredients that have the potential to have biological activity as an antiplatelet is papaya leaves (Noviar, 2023). Flavonoid compounds, steroids and tannins in free form and tannin-protein complexes have anti-aggregation properties. This research uses 2 methods, namely survey and sampling methods to determine the scope of empowerment and education in the form of counseling and assistance. Apart from that, another method used is an educational method in the form of counseling and assistance to provide an understanding of the use of papaya leaves to prevent the risk of blood vessel blockages. The results of research from 18 cadres showed that based on the Wilcoxon test, a sig value of 0.000 <α (0.05) was obtained. Thus, it can be concluded that the pre-test scores and post-test scores have a significant difference in scores with an increase in scores for cadres of 22.66% or 40.02%. Meanwhile, the results of the Paired T Test obtained a sig value of 0.000 <α (0.05), thus it can be concluded that the platelet aggregation value before and after administration of papaya leaves had a significant difference with a decrease in value of 9.94% or 13.87%. This shows that papaya leaves are effective in reducing platelet aggregation to prevent the risk of blood vessel blockages
Assessment of Analytical Specificity in qPCR: SYBR Green and TaqMan Probe Methods for Isonazid-Resistant Mycobacterium tuberculosis Merdekawati, Fusvita; Suryawan, Muhammad Raihan; Nurhayati, Betty; Rinaldi, Sonny Faisal; Juliastuti, Aditya
Mukhtabar Journal of Medical Laboratory Technology Vol 3 No 1 (2025): Mukhtabar: Journal of Medical Laboratory Technology (April 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v3i1.793

Abstract

Background & Objective: Mycobacterium tuberculosis, often referred to as M. tuberculosis, is an infectious pathogen that is responsible for causing tuberculosis (TB), a prevalent condition that is a leading cause of death globally. The spread of tuberculosis bacteria that are resistant to certain medicines, such as isoniazid, is currently on the rise. Therefore, molecular testing, such as quantitative polymerase chain reaction (qPCR), is needed to rapidly and reliably identify isoniazid-resistant tuberculosis germs. Method: The purpose of this study was to assess the analytical specificity of TaqMan Probe and SYBR Green qPCR techniques for the detection of isoniazid-resistant tuberculosis. This study was descriptive and quantitative. Analytical specificity was ascertained using MTB DNA with the S315G mutation spiked with E. coli DNA. A paired t-test was used to assess the primary data. Result: The results show that the analytical specificity values based on the significance of the paired t-test for the SYBR Green and TaqMan Probe methods were 0.398 and 0.790, respectively. Conclusion: Based on these results, it can be concluded that the analytical specificity of the TaqMan Probe qPCR method was 1.99 times greater than that of the SYBR Green method.
Co-Authors Abror, Yogi Khoirul Arbie, Arbie Candra Purnawan Dermawan, Asep Didit Damayanti Djuminar, Ai Durachim, Adang Edi Pramono Eka Setiawan, Regina Endarwati, Dwi Veni Hardiana, Acep Tantan Hayati, Eem Ida Widiawati, Ida Ihsan Nurhadi, Muhammad Indra, Asep Iin Nur Juliastuti, Aditya Kurnia Kusniasih, Susi Maryani, Nita Merdekawati, Fusvita Nina Marliana, Nina Noviar, Ganjar Nur Ramadhani, Riski Nurhadi, Muhammad Ihsan Ozi Adi Saputra, Ozi Adi Pujiasih, Septi Ramadhani, Riski Nur Ridwanna, Surya Rinaldi, Sonny Faisal Rinaldi, Sonny Feisal Rizki, Vanani Nur Rumahorbo, Hotma Saputra, Fitrianingsih Sasmita, Anah Simangunsong, Kristina Suhartini, Entin Suryawan, Muhammad Raihan Tania, Monica Thayyiba, Amina Wardhani, Azkya Zhahira