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All Journal Jurnal Fitopatologi Indonesia JURNAL TEKNOLOGI LINGKUNGAN Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA
FAJARUDIN AHMAD
Department of Biology, Faculty of Mathematic and Natural Science, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia Research Center for Biology, Indonesia Institute of Science (LIPI), Jalan Raya Jakarta Bogor Km. 46, Cibinong 16
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Medicine & Pharmacology

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Journal : BERITA BIOLOGI

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ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR [Genetic variation analyses of Musa balbisiana Colla based on RAPD and ISSR markers] Poerba, Yuyu Suryasari; Ahmad, Fajarudin
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.81 KB) | DOI: 10.14203/beritabiologi.v12i2.540

Abstract

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99.
ANALISIS KERAGAMAN GENETIK KENTANG HITAM Yulita, Kusumadewi Sri; Ahmad, Fajarudin; Martanti, Diyah; Poerba, Yuyu S.; Herlina, Herlina
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.686

Abstract

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living in some parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity. This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initially screened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835) generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20% (UBC 807 and 834) to 16.39% (OPB 10) with an average of 12.50%polymorphism.Clustering analysis was performed based on ISSR and RAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity among accessions was 51-100% to which most of the accessions were clustered with more than 80% similarity. This confirmed our hypothesis of the low level of variation existed among accessions.
Co-Authors Diyah Martanti, Diyah Harahap, Elmira Oktaria Raihan Herlina Herlina KUSUMADEWI SRI YULITA Nani, Maryani Rida Oktorida Khastini Witjaksono Witjaksono Yuyu S. Poerba, Yuyu S. Yuyu Suryasari Poerba