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All Journal Science Midwifery Cermin Dunia Kedokteran
Angeline, Wiedya Kristianti
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Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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CRISPR - Inovasi Biologi Molekuler dan Medis yang Kontroversial Angeline, Wiedya Kristianti
Cermin Dunia Kedokteran Vol 47, No 3 (2020): Dermatologi
Publisher : PT. Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1107.568 KB) | DOI: 10.55175/cdk.v47i3.375

Abstract

CRISPR, sebuah teknologi pengubah gen telah terbukti mampu mengubah, memperbaiki, mengganti bagian gen dengan tujuan melakukan terobosan genetik untuk hal preventif dan terapeutik serta meningkatkan kualitas hidup manusia. CRISPR merupakan harapan baru, namun masih perlu diperhatikan implikasi sosial dan dampak etisnya. Penelitian lebih lanjut diperlukan untuk memperjelas keamanan dan spesifisitas teknik tersebut.CRISPR, a gene-modifying technology has been proven to be able to change, repair, replace parts of genes as a genetic breakthrough in prevention and therapeutic as well as in improving the quality of human life. CRISPR is a new hope, but its social implications and ethical impacts still needs to be considered. Further research is needed to clarify the safety and specificity of this technique.
TNF-α and IL-10 as paracrine effect of encapsulated mesenchymal stem cells coating by platelet lysate Sibuea, Christine Verawaty; Sitanggang, Ervina Julien; Simaremare, Ade Pryta; Silaen, Rachel Teodora; Kuara , Glenessa; Samosir, Sarah Christina; Ginting, Kharnis Marsha Madora; Yana, Hiqmah Yusi; Pratama, Gita; Mutiara, Mutiara; Angeline, Wiedya Kristianti
Science Midwifery Vol 11 No 6 (2024): February: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i6.1427

Abstract

Mesenchymal stem cells (MSCs) have been used as a cellular therapy for infectious and degenerative diseases due to their paracrine effect, immunomodulatory capability, high ability differentiation, and high plasticity. The paracrine effect of MSCs releases many growth factors and pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), enabling them to modulate the immune system. Nevertheless, there are many obstacles to maintaining paracrine effects in cellular therapy due to a shortage of cellular retention. MSC encapsulation provides a favourable environment for the enhanced viability of MSCs. Platelet lysate is comprised of many growth factors that support the paracrine effect of mesenchymal stem cells (MSCs). In this study, MSCs were encapsulated within alginate, crosslinked using calcium chloride (CaCl2), and subsequently coated with platelet lysate. Encapsulated MSCs coated by platelet lysate were cultured for 21 days and analyzed for IL-10 and TNF-α levels. The findings of our study performed that TNF-α in encapsulated mesenchymal stem cells (MSCs) coated with platelet lysate increased until day 21. IL-10 was retained within the capsule and detected very in day 14. This study showed that encapsulated MSCs coated with platelet lysate affected paracrine effect TNF-α of MSC and retained IL-10 inside the capsule
Co-Authors Christine Verawaty Sibuea Ginting, Kharnis Marsha Madora Gita Pratama Kuara , Glenessa mutiara mutiara Samosir, Sarah Christina Silaen, Rachel Teodora Simaremare, Ade P Sitanggang, Ervina Julien Yana, Hiqmah Yusi