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Kuara , Glenessa
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Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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TNF-α and IL-10 as paracrine effect of encapsulated mesenchymal stem cells coating by platelet lysate Sibuea, Christine Verawaty; Sitanggang, Ervina Julien; Simaremare, Ade Pryta; Silaen, Rachel Teodora; Kuara , Glenessa; Samosir, Sarah Christina; Ginting, Kharnis Marsha Madora; Yana, Hiqmah Yusi; Pratama, Gita; Mutiara, Mutiara; Angeline, Wiedya Kristianti
Science Midwifery Vol 11 No 6 (2024): February: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i6.1427

Abstract

Mesenchymal stem cells (MSCs) have been used as a cellular therapy for infectious and degenerative diseases due to their paracrine effect, immunomodulatory capability, high ability differentiation, and high plasticity. The paracrine effect of MSCs releases many growth factors and pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), enabling them to modulate the immune system. Nevertheless, there are many obstacles to maintaining paracrine effects in cellular therapy due to a shortage of cellular retention. MSC encapsulation provides a favourable environment for the enhanced viability of MSCs. Platelet lysate is comprised of many growth factors that support the paracrine effect of mesenchymal stem cells (MSCs). In this study, MSCs were encapsulated within alginate, crosslinked using calcium chloride (CaCl2), and subsequently coated with platelet lysate. Encapsulated MSCs coated by platelet lysate were cultured for 21 days and analyzed for IL-10 and TNF-α levels. The findings of our study performed that TNF-α in encapsulated mesenchymal stem cells (MSCs) coated with platelet lysate increased until day 21. IL-10 was retained within the capsule and detected very in day 14. This study showed that encapsulated MSCs coated with platelet lysate affected paracrine effect TNF-α of MSC and retained IL-10 inside the capsule
Kadar TNF ( Mikroenkapsulasi Sel Punca Hematopoietik CD34+ Sibuea, Christine Verawaty; Sibarani, Joseph Partogi; Silaen, Rachel Teodora; Samosir, Christina br; Kuara , Glenessa; Ginting, Kharnis Marsha Madora
Jurnal Ners Vol. 7 No. 2 (2023): OKTOBER 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v7i2.26029

Abstract

Sel punca hematopoietik CD34+ memiliki memiliki kemampuan angiogenik, plastisitas dan sebagai imunoregulator. Hal ini menyebabkan sel punca hematopoietik CD34+ banyak digunakan pada terapi seluler. Rendahnya kemampuan proliferasi dan rendahnya viabilitas migrasi ke host, memberikan keterbatasan pendekatan terapi seluler yang menggunakan sel punca hematopoietik CD34+. Enkapsulasi mempertahankan fungsi sel punca hematopoietik CD34+ dan dapat meregulasi sistem imun. Tumor Necrosis Factor ( (TNF() merupakan molekul pro-inflamasi yang berperan penting dalam imunoregulasi. TNF( berperan penting dalam reaksi penolakan transplantasi sel punca hematopoietik CD34+. Penelitian ini menganalisa kadar TNF(pada enkapsulasi sel punca hematopoietik CD34+. Sel punca hematopoietik CD34+ dikapsulasi dengan menggunakan alginate cross linked dengan CaCl2, dan dikultur hingga 21 hari. Kadar TNF( dianalisa dengan metode ELISA. Kadar TNF( menurun pada hari ke-7 dan tidak ditemukan hingga hari ke-21. Enkapsulasi menurunkan kadar TNF( dan dapat digunakan untuk pengembangan terapi seluler sel punca hematopoietik CD34+.
Co-Authors Angeline, Wiedya Kristianti Christine Verawaty Sibuea Ginting, Kharnis Marsha Madora Gita Pratama Joseph Partogi Sibarani mutiara mutiara Samosir, Christina br Samosir, Sarah Christina Silaen, Rachel Teodora Simaremare, Ade P Sitanggang, Ervina Julien Yana, Hiqmah Yusi