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The Effect of Various Photoperiodic Conditions and Zn2+ Concentrations on Growth Rate and Metabolite Content in Euglena sp: Effect of Photoperiod and Zn2+ on Euglena sp. Eko Agus Suyono; Budiman, Arief; Siti Ferniah, Rejeki; Astiti, Adam; Mardyansah, Deviko; Natalia, Fitri; Cindiati, Maya; Qonita Maghfiroh, Khusnul; Erfianti, Tia; Nurafifah, Istini; Amelia, Ria; Kurnianto, Dedy; Ryan Sadewo, Brilian; Maggandari, Revata
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.04

The application of Euglena as a carbon capture organism has generated considerable interest among scientists. Through the photosynthesis process, many kinds of metabolites are produced by Euglena, such as lipids, proteins, and pigments. Due to the metabolites produced by Euglena, it is vital to optimize the carbon capture ability and cell growth rate by adding Zn2+ content and giving photoperiodic into Euglena culture. The purpose of this study is to identify the optimal photoperiod and Zn2+ concentration to increase the growth rate, biomass, and metabolite content of Euglena sp. This study is a laboratory experiment involving the cultivation of Euglena sp. in various photoperiod cycles (light:dark), namely 24:0, 12:12, 14:10, and 16:8. In addition, Euglena sp. was also cultivated using different concentrations of Zn2+ (0 ppm, 5 ppm, 10 ppm, and 15 ppm). The growth of Euglena sp. was monitored for 18 days before being harvested every three days to measure the research parameters, including primary and secondary metabolites. The results showed that the photoperiod treatment and various concentrations of Zn2+ had a significant impact (P<0.05) on the growth rate, biomass, lipid, carbohydrate, protein, chlorophyll, and carotenoid levels of Euglena sp.

Exploration of Lipid Profile and Wax Ester Content from Local Strain Euglena sp. IDN33 Cultivated in Mixotrophic Condition Combined with Molasses Supplementation Astiti, Adam; Erfianti, Tia; Maghfiroh, Khusnul Qonita; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.805-818

The transition from fossil fuels to sustainable renewable energy is currently growing rapidly. Euglena sp. can be the source of biofuel. Molasses, in addition to Euglena sp., can increase the levels of paramylon and wax esters produced. Therefore, this study aims to analyze cell growth, lipid profile, and wax ester content of Euglena sp. cultivated under mixotroph conditions with molasses. Euglena was cultivated under mixotrophic conditions with the addition of various concentrations of molasses (0, 10, 15, and 20 g/L) until the final log phase. Then, measurements were made on cell density, levels of lipids, proteins, and paramylon to measure the growth and metabolites profile of Euglena sp., along with lipid profile and wax ester analysis using GC-MS. The results of the study showed that 10 g/L molasses increased Euglena growth (SGR 0.1736± 0.0213 mg/ml) and carbohydrate (0.426 mg/ml) but reduced lipid accumulation (2.29 mg/ml). This treatment provides the best result before control. Molasses does not increase carbohydrates and protein but increases paramylon accumulation. The GC-MS assay detected more lipid profiles and concentrations of each lipid type in non-molasses-treated Euglena sp. compared to molasses-treated. Euglena was cultivated on molasses, which was dominated by palmitic acid and myristyl myristate with the absence of PUFA-type fatty acids in the cells, while the untreated ones were dominated by stearic acid and myristyl myristate. Thus, 10 g/L supplementation of molasses is the most effective treatment compared to others.

The Effect of Various Photoperiodic Conditions and Zn2+ Concentrations on Growth Rate and Metabolite Content in Euglena sp: Effect of Photoperiod and Zn2+ on Euglena sp. Eko Agus Suyono; Budiman, Arief; Siti Ferniah, Rejeki; Astiti, Adam; Mardyansah, Deviko; Natalia, Fitri; Cindiati, Maya; Qonita Maghfiroh, Khusnul; Erfianti, Tia; Nurafifah, Istini; Amelia, Ria; Kurnianto, Dedy; Ryan Sadewo, Brilian; Maggandari, Revata
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.04

The application of Euglena as a carbon capture organism has generated considerable interest among scientists. Through the photosynthesis process, many kinds of metabolites are produced by Euglena, such as lipids, proteins, and pigments. Due to the metabolites produced by Euglena, it is vital to optimize the carbon capture ability and cell growth rate by adding Zn2+ content and giving photoperiodic into Euglena culture. The purpose of this study is to identify the optimal photoperiod and Zn2+ concentration to increase the growth rate, biomass, and metabolite content of Euglena sp. This study is a laboratory experiment involving the cultivation of Euglena sp. in various photoperiod cycles (light:dark), namely 24:0, 12:12, 14:10, and 16:8. In addition, Euglena sp. was also cultivated using different concentrations of Zn2+ (0 ppm, 5 ppm, 10 ppm, and 15 ppm). The growth of Euglena sp. was monitored for 18 days before being harvested every three days to measure the research parameters, including primary and secondary metabolites. The results showed that the photoperiod treatment and various concentrations of Zn2+ had a significant impact (P<0.05) on the growth rate, biomass, lipid, carbohydrate, protein, chlorophyll, and carotenoid levels of Euglena sp.

Morfologi Trikoma Petal Dan Sepal Bererapa Varietas Bunga Krisan (Chrysanthemum Morifolium Ramat.) Utaminingsih, Utaminingsih; Astiti, Adam; Sutikno, Sutikno
Al-Hayat: Journal of Biology and Applied Biology Vol. 4 No. 2 (2021)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v4i2.6861

Krisan merupakan tumbuhan yang termasuk dalam familia Acanthacea yang hidup pada dataran tinggi dengan iklim tropis atau sub tropis. Krisan digunakan sebagai obat herbal karena mengandung senyawa flavonoid dan alkaloid yang digunakan sebagai anti antibakteri, antiinflamasi, antioksidan. Berdasarkan pendekatan anatomi dapat diketahui bahwa tumbuhan mensintesis dan menyimpan senyawa bioaktif melalui sel-sel dan jaringan yang terspesialisasi. Salah satu sel, dan jaringan yang terspesialisasi untuk mensekresi metabolit sekunder adalah trikoma. Trikoma dapat dibagi menjadi trikoma glandular dan non glandular. Trikoma glandular dapat menyimpan dan mensekresi metabolit sekunder pada tumbuhan, sedangkan trikoma non glandular tidak dapat mensekresi metabolit sekunder. Tujuan dari penelitian ini adalah mengetahui bentuk, dan jenis trikoma pada varietas krisan fiji kuning, fiji putih, jimla kuning, puspita nusantara dan barcadi. Penelitian ini menggunakan metode leaf clearing pada sepal dan petal bunga krisan, kemudian dilakukan pengamatan dengan optilab. Pada penelitian ini ditemukan bahwa terdapat lima jenis trikoma glandular dan dua jenis trikoma non glandular. Dari penelitian yang telah dilakukan dapat disimpulkan bahwa terdapat tujuh tipe trikoma yang ditemukan pada lima varietas krisan yang diuji dan setiap varietas krisan memiliki jenis trikoma yang berbeda.

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