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All Journal HAYATI Journal of Biosciences Makara Journal of Science Journal of Fisheries & Marine Biosaintifika: Journal of Biology & Biology Education
Putri, Renata Adaranyssa Egistha
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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The Effect of Acidic pH on Growth Kinetics, Biomass Productivity, and Prima-ry Metabolite Contents of Euglena sp. Nurafifah, Istini; Hardianto, Muhammad Andhi; Erfianti, Tia; Amelia, Ria; Maghfiroh, Khusnul Qonita; Kurnianto, Dedy; Siswanti, Dwi Umi; Sadewo, Brilian Ryan; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
Makara Journal of Science Vol. 27, No. 2
Publisher : UI Scholars Hub

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Abstract

Euglena is a microalga with the potential to be an environmentally friendly renewable energy resource. The pH value is a crucial factor in micro-algal cultivation. Changes in pH affect the growth and development of microalgae, including the production of biomass and primary metabolites, such as proteins, carbohydrates, and lipids. In this study, Euglena sp. was grown on Cramer-Myers medium and subjected to various acidic conditions. This study aimed to determine the effect of pH on the growth kinetics, biomass, carbohydrate, lipid, and protein contents of Euglena sp. The Euglena sp. culture was optimized at various pH values of 2.5, 3.5, and 4.5. The results were analyzed by one-way analysis of variance at a 95% confidence level, followed by Duncan’s multiple range test. As results, Euglena sp. had the best growth rate, the greatest biomass, and the highest carbohydrate, protein, and lipid contents at pH 3.5 compared to the other pH conditions. The average biomass in the pH 3.5 treatment was 1.600 ± 0.229 g/L, and the carbohydrate, protein, and lipid contents were 5.983 ± 0.056 g/L, 0.196 ± 0.023 µg/mL, and 0.300 ± 0.020 g/L, respectively.
Exploration of Lipid Profile and Wax Ester Content from Local Strain Euglena sp. IDN33 Cultivated in Mixotrophic Condition Combined with Molasses Supplementation Astiti, Adam; Erfianti, Tia; Maghfiroh, Khusnul Qonita; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.805-818

Abstract

The transition from fossil fuels to sustainable renewable energy is currently growing rapidly. Euglena sp. can be the source of biofuel. Molasses, in addition to Euglena sp., can increase the levels of paramylon and wax esters produced. Therefore, this study aims to analyze cell growth, lipid profile, and wax ester content of Euglena sp. cultivated under mixotroph conditions with molasses. Euglena was cultivated under mixotrophic conditions with the addition of various concentrations of molasses (0, 10, 15, and 20 g/L) until the final log phase. Then, measurements were made on cell density, levels of lipids, proteins, and paramylon to measure the growth and metabolites profile of Euglena sp., along with lipid profile and wax ester analysis using GC-MS. The results of the study showed that 10 g/L molasses increased Euglena growth (SGR 0.1736± 0.0213 mg/ml) and carbohydrate (0.426 mg/ml) but reduced lipid accumulation (2.29 mg/ml). This treatment provides the best result before control. Molasses does not increase carbohydrates and protein but increases paramylon accumulation. The GC-MS assay detected more lipid profiles and concentrations of each lipid type in non-molasses-treated Euglena sp. compared to molasses-treated. Euglena was cultivated on molasses, which was dominated by palmitic acid and myristyl myristate with the absence of PUFA-type fatty acids in the cells, while the untreated ones were dominated by stearic acid and myristyl myristate. Thus, 10 g/L supplementation of molasses is the most effective treatment compared to others.
Effect of Red and Blue Light on Lipid, Protein, Carbohydrate, and Pigment Contents of Navicula sp. Maulana, Sofyan; Nurul Hida, Aulia Setyo; Wulandari, Ismia; Mudrikah, Siti; Kurnianto, Dedy; Maghfiroh, Khusnul Qonita; Amelia, Ria; Erfianti, Tia; Putri, Renata Adaranyssa Egistha; Devi Anggraini, Irika; Marno, Septhian; Suyono, Eko Agus Agus
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

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Light quality critically modulates the physiology of diatoms, influencing photosynthetic efficiency and metabolite synthesis. This study examines how monochromatic red (620–750 nm) or blue (450–495 nm) light affects the biochemical profiles and pigment production in Navicula sp. versus white light (control). Cultures were grown in sterile f/2 medium, and biomass was tracked via OD680. Biochemical analyses included lipids (Bligh and Dyer method), carbohydrates (phenol-sulfuric acid assay), proteins (Bradford assay), and pigments (spectrophotometric quantification). The growth dynamics were modeled using logistic and Gompertz equations. No significant differences in OD-based growth rates, carbohydrates, proteins, or lipids content were observed with red or blue light compared with the control. However, blue light elevated chlorophyll a levels 4-fold and maximized protein productivity 3-fold, whereas red light enhanced carotenoid content by 20.5-fold. The growth kinetics modeling that best matched the experimental data was the Gompertz modeling (R² > 0.649). These results demonstrate the wavelength-specific regulation of pigment and protein yields, suggesting that tailored light regimes can selectively optimize high-value metabolites in Navicula sp. This approach holds promise for biotechnology sectors prioritizing targeted compound production, such as nutraceuticals or biofuels, by leveraging spectral tuning to enhance metabolic efficiency and predicting particular growth phases using growth kinetics.
Harvesting Optimization, Biomass, and Lipid Content Analysis of Euglena sp. Culture with Ettlia texensis Bioflocculant and Commercial Chitosan Agus Suyono, Eko; Mudrikah, Siti; Karilanata, Khalid Erlangga; Kurnianto, Dedy; Budiman, Arief; Dewayanto , Nugroho; Putri, Renata Adaranyssa Egistha; Sadewo , Brilian Ryan; Erfianti, Tia
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 2 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i2.57417

Abstract

Graphical Abstract   Highlight Research  Bioflocculation technique can improve the harvesting effectiveness of semimass culture of Euglena sp. The addition of E. texensis can significantly increase the flocculation efficiency of Euglena sp. The addition of commercial chitosan was able to increase the flocculation efficiency of Euglena sp. The biomass and lipid content produced by Euglena sp. with E. texensis flocculant agent showed higher results than the biomass and lipid content produced by Euglena sp. with commercial chitosan flocculant agent.   Abstract Euglena sp. has a high potential to be developed as biofuel. However, the high cost and energy required for the harvesting process are hindering the production. Flocculation using natural substances, such as microorganisms and biopolymers, offers a promising solution to minimize energy and production costs, so it is applicable on a mass scale. Ettlia texensis is one of the autoflocculating microalgae that can excrete extracellular polymeric substances (EPS). Chitosan is a linear copolymer of D-glucosamine and N-acetyl-D-glucosamine produced by the deacetylation of chitin, which is usually exploited by marine crustaceans, shrimp, and crabs. Chitosan has a very high cation load, so it is often used for coagulation or flocculation. This study explores the potential of E. texensis and chitosan as flocculant agents to harvest the mass culture of Euglena sp. by giving different doses E. texensis with 1:0.25 (E3), 1:0.5 (E4), 1:1 (E5), and 1:2 (E6), and chitosan with 1.25 mg (C1), 2.5 mg (C2), 3.75 mg (C3), and 5 mg (C4). This research began with the cultivation of Euglena sp. and E. texensis on a 50 L scale for 12 days. The effectiveness of flocculation was measured by the spectrophotometric method. Based on this research, the best treatment for harvesting Euglena sp. culture by bioflocculation was shown by the addition of chitosan (5 mg) with the recovery of 84.83%, 0.2213 mg/mL biomass, and 0.2117 mg/mL lipid content. Meanwhile, with E.  texensis, the best was shown by the ratio of 1:2 with recovery 84.71%, 0.2053 mg/mL biomass, and 0.1753 mg/mL
Effect of Light Intensity on Growth and Primary Metabolites Content of Navicula sp. Mudrikah, Siti; Putri, Renata Adaranyssa Egistha; Kurnianto, Dedy; Rasdi, Abdurrahman Muhammad Fikri; Tia, Erfianti; Suyono, Eko Agus
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.13899

Abstract

Global energy demand is steadily rising, necessitating the exploration of sustainable alternatives. Microalgae, such as Navicula sp., offer a promising solution. This study investigated the impact of light intensity on the growth, and biochemical composition (carbohydrates, lipids, proteins, and carotenoids) of Navicula sp. Cultures were grown in f/2 medium under four light intensity treatments: 2100 lux (control), 3500 lux, 4500 lux, and 5500 lux. Cell growth was measured through optical density analysis. Carbohydrate, lipid, protein, and carotenoid assays were performed using phenol-sulfuric acid, Bligh and Dyer, Bradford, and spectrophotometric methods. Maximum growth was observed on the eighth day of cultivation at 4500 lux light intensity. The best specific growth rate was achieved at 5500 lux. The highest biomass, carbohydrate, lipid, and protein productivity were attained at 5500 lux (24.65 mg/mL, 0.374 mg/mL/day, 0.399±0.204 mg/mL/day, and 0.025±0.018 mg/mL/day). Conversely, the highest carotenoid productivity was recorded at 2100 lux, 0.278±0.085 mg/mL/day. By those means, the increase in light intensity was positively correlated with microalgal cell growth, biomass, carbohydrate content, and carbohydrate, lipid, and protein productivity. However, a negative correlation was found with carotenoid pigment productivity. From this research, it is evident that a light intensity of 5500 lux offers a feasible option for the industrial-scale cultivation of Navicula sp. microalgae for biomass, carbohydrate, and protein production, while 2100 lux is more suitable for carotenoid production. This research compares the findings to previous studies, emphasizing the unique way Navicula sp. responds to different light levels.
Co-Authors Agus Suyono, Eko Amelia, Ria Arief Budiman Astiti, Adam Devi Anggraini, Irika Dewayanto , Nugroho Dwi Umi Siswanti Eko Agus Suyono Erfianti, Tia Hardianto, Muhammad Andhi Karilanata, Khalid Erlangga Kurnianto, Dedy Maghfiroh, Khusnul Qonita Marno, Septhian Maulana, Sofyan Nurafifah, Istini Nurul Hida, Aulia Setyo Rasdi, Abdurrahman Muhammad Fikri Sadewo , Brilian Ryan Sadewo, Brilian Ryan siti mudrikah Solly Aryza Suyono, Eko Agus Agus Tia, Erfianti Wulandari, Ismia