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All Journal INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Indonesian Journal of Tropical and Infectious Disease Ovozoa: Journal of Animal Reproduction
Bimo Aksono
Institute of Tropical Diseases, Universitas Airlangga, Surabaya. Faculty of Veterinary, Universitas Airlangga, Surabaya.
Agriculture, Biological Sciences & Forestry Arts Humanities Computer Science & IT Earth & Planetary Sciences Environmental Science Health Professions Medicine & Pharmacology Public Health Veterinary

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 1 
The Effect of Gendarussin a Isolates of Justicia gendarussa Burm.f. Leaf in Reverse Transcriptase Inhibition of HIV Type I In Vitro E. W., Bambang Prajogo; Widiyanti, Prihartini; Nasronudin, Nasronudin; Aksono, Bimo
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Screening has been done to a few extracts from the leaves Justicia gendarussa Burm.f to see the growth rate of the virus from the blood plasma of HIV patients at Dr Soetomo Hospital. It is known that J. gendarussa leaf extract inhibits HIV type 1 reverse transcriptase. In addition, its main content is gendarussin A, besides gendarussin B, JGF1, JGF2 and JGF3, which have just identified. At the beginning, extraction and fractionation were performed with 3 models that highlight the absolute methanol, 70% methanol and 70% ethanol with the release of alkaloids. Furthermore, samples of each fraction were incubated in plasma of HIV patients with a titer of 3.6 10^6 copies for 1 h in concentrations of 1.64 ppm, 4.1 ppm, 8.2 ppm, 16.4 ppm and 41.0 ppm. After incubation, examination was performed by using Nucli sens a machine, which is a combination of PCR and Elisa, thus avoiding direct contact with the highly pathogenic virus. The result showed that the activity sequence from the most potential to the weak, among others, was 1.64 ppm >4.1 ppm > 8.2 ppm > 16.4 ppm > 41.0 ppm, each with barriers value of 0.62 10^6, 1.4 10^6, 1.6 10^6, 2.4 10 cells/ml. In conclusion, highest anti-HIV activity comes from the concentration of gendarussin A isolate at 1.64 ppm. Furthermore, after linearregression of y = -3.063 x + 81.37 was done, the IC50 of 10.24 ppm was obtained.
Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara Adriaty, Dinar; Wahyuni, Ratna; Iswahyudi, Iswahyudi; Aksono, Bimo; Koesbardiati, Toetik; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.2.3.%x

Abstract

The existence of leprosy or kusta or Morbus Hansen or Hansens disease has been known for years, including in Indonesia. Starting from the discovery of Mycobacterium leprae isolates from ancient bone (about 1.000 years B.C), the archaeological excavations results in East Nusa Tenggara, interesting questions arise about how the development of leprosy in eastern Indonesia is. Biology molecular study would become a powerful tool to investigate the presence of leprosy bacillary whether there are similarities between the genomes of M. leprae isolates in the primeval and the present. PCR examinations were performed on mandibular bone fragments from ancient human who lived 1000 years B.C. discovered in archaeological surveys on the island of Lembata and three leprosy patients from East Nusa Tenggara. The DNA extraction was performed using a kit from Qiagen products and its TTC repeating pattern was seen with the method of direct sequencing. It turned out that the TTC profile obtained from samples of archaeological was as many as 13 copies, while the repetition of TTC in three samples of leprosy patients were 15, 17 and 26 copies. The different number of TTC repetition shows the different isolates of M. leprae between in the ancient times and the present. Further studies are needed to verify the differences in the genome that occur, for example from the study of SNPs (single nucleotide polymorphisms).
THE EFFECT OF GENDARUSSIN A ISOLATES OF Justicia gendarussa Burm.f. LEAF IN REVERSE TRANSCRIPTASE IN HIBITION OF HIV TYPE I INVITRO E. W., Bambang Prajogo; Widiyanti, Prihartini; Nasronudin, Nasronudin; Aksono, Bimo
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 5 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.76 KB) | DOI: 10.20473/ijtid.v5i5.307

Abstract

Screening has been done to a few extracts from the leaves Justicia gendarussa Burm.f to see the growth rate of the virus from the blood plasma of HIV patients at Dr Soetomo Hospital. It is known that J. gendarussa leaf extract inhibits HIV type 1 reverse transcriptase. In addition, its main content is gendarussin A, besides gendarussin B, JGF1, JGF2 and JGF3, which have just identified. At the beginning, extraction and fractionation were performed with 3 models that highlight the absolute methanol, 70% methanol and 70% ethanol with the release of alkaloids. Furthermore, samples of each fraction were incubated in plasma of HIV patients with a titer of 3.6 10^6 copies for 1 h in concentrations of 1.64 ppm, 4.1 ppm, 8.2 ppm, 16.4 ppm and 41.0 ppm. After incubation, examination was performed by using Nucli sens a machine, which is a combination of PCR and Elisa, thus avoiding direct contact with the highly pathogenic virus. The result showed that the activity sequence from the most potential to the weak, among others, was 1.64 ppm >4.1 ppm > 8.2 ppm > 16.4 ppm > 41.0 ppm, each with barriers value of 0.62 10^6, 1.4 10^6, 1.6 10^6, 2.4 10 cells/ml. In conclusion, highest anti-HIV activity comes from the concentration of gendarussin A isolate at 1.64 ppm. Furthermore, after linearregression of y = -3.063 x + 81.37 was done, the IC50 of 10.24 ppm was obtained.
CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI PERAH AKSEPTOR INSEMINASI BUATAN DI KUD ARGOPURO KECAMATAN KRUCIL KABUPATEN PROBOLINGGO Yohana, Nadia; Samik, Abdul; Aksono, Bimo; Sardjito, Trilas; Hermadi, Herry Agoes; Restiadi, Tjuk Imam
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.663 KB) | DOI: 10.20473/ovz.v7i2.2018.143-147

Abstract

Reproduction is one of essential factor directly affecting profitability in a dairy production system. Some of the reproductive factors that influenced to the developing of dairy cattle of small holder's farmer are Conception Rate (CR) Service per Conception (S/C). The study was conducted to know the CR and S/C of Friesian Holstein in KUD Argopuro, Probolinggo. The data is taken primary and secondary data. Primary data retrieval is done by direct observation, which includes several variables, where the variables include : the identity of the breeder, cages, and feeding and drinking. As for the secondary data obtained by recording the card Artificial Insemination (AI) owned by Inseminator in KUD Argopuro. The data which by collected from this sample are CR and S/C. This research was a case study. The materials used cattle as the acceptors were 10% from 728 lactation cattle. Data from the results of this research on CR (30%) and S/C (2,49). The conclusion of this research for CR the result were lower than normal and S/C the result were higher than normal.
PENGENDALIAN DAN PENINGKATAN KUALITAS HIDUP PENDERITA LEPRA BERBASIS TEKNOLOGI MOLEKULER DI UNIT UJI TROPICAL DISEASE DIAGNOSTIC CENTRE UNIVERSITAS AIRLANGGA Aksono, Bimo
INOTEKS: Jurnal Inovasi Ilmu Pengetahuan,Teknologi, dan Seni Vol 14, No 1 (2010)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1177.116 KB) | DOI: 10.21831/ino.v14i1.2288

Abstract

The aim of community service activities through a U-UJI TDDC will be conducted strain mapping M. leprae (genetic mapping) and TTC-based maping region mutation patterns of drug rifampin rpoB gene-based regions. The be- nefits of this activity is an increase in quality of life for the leprosy both socially and economically because of the application of molecular technology could help  early  detection  of  regional-based  Variable  Number  Tandem  Repeat (VNTR) of the TTC and rifampin-resistant gene (rpoB gene) and is expected to occur later therapeutic action will accelerate the decline in the incidence and cure the patient. Materials in this study isolates M. leprae from skin incision swabs, nasal mucosa and skin lesions biopisi of leprosy who come from a few enclaves leprosy in  Sumberaji-Lamongan District.  Criteria  for  patient  sample  is  still relatively new or are in treatment. Based on these criteria finally set 17 sample patients, whether included Pseudobasiler type (1 sample) and Multibasiler (16 samples). The method used is a nested PCR using specific primers from the region of the TTC, LP and rpoB. The results of nucleotide sequencing of both the sequence of the region of TTC, LP and rpoB region using GENETIX Mac software ver. 9.0. Community service activities based on the application of molecular technology by U-Uji TDDC, Airlangga University in endemic areas, especially infectious diseases in leprosy Sumberaji Lamongan District, from the results of PCR and nucleotide sequence analysis of a sample selected patients  indicates that the bacteria M. leprae in Sumberaji consists of three strains of M. leprae based on TTC-15, TTC-23 and TTC-28, while the analysis of the rpoB region of the mutation was not found either in CAG513-Gln, GAT516-Asp, CAC526- His, and TCG531-Ser-Leu CTG533, this indicates that the isolates of   M . leprae found in the leprosy in Sumberaji Lamongan district are sensitive to rifampin treatment. The results of this mapping can be used as a reference that rifampin  treatment  can  be used  for  therapy MDT  on  leprosy in  Sumberaji Lamongan district
Co-Authors Abdul Samik, Abdul Bambang Prajogo DINAR ADRIATY Herry Agoes Hermadi INDROPO AGUSNI ISWAHYUDI ISWAHYUDI Nasronudin Nasronudin Prihartini Widiyanti RATNA WAHYUNI SHINZO IZUMI Tjuk Imam Restiadi Toetik Koesbardiati Trilas Sardjito Yohana, Nadia