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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Microbiology Indonesia Berkala Ilmu Kesehatan Kulit dan Kelamin Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Ilmu Kesehatan dan Gizi
INDROPO AGUSNI
Dept. Of Dermatology, School Of Medicine, Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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COMPARATIVE STUDY ON THE INTENSITY OF Mycobacterium leprae EXPOSURE TO CHILDREN WHO LIVE IN LOW AND HIGH ALTITUDE IN LOW LEPROSY ENDEMIC AREA OF SOUTH SULAWESI Rachmawati, Rachmawati; Mataallo, Timurleng Tonang; Adam, Safruddin; Adam, A. M.; Amin, Safruddin; Tabri, Farida; Adriaty, Dinar; Wahyuni, Ratna; Iswahyudi, Iswahyudi; Agusni, Indropo
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

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Abstract

Background: The intensity of Mycobacterium leprae exposure to people who live in leprosy endemic area could be measured by serological study and detection of the bacilli in the nose cavity. Different geographical altitude might have some influences to this exposure since the bacilli prefer to live in warm areas. Aim: A combined serological and PCR study of leprosy was conducted in Selayar island, South Sulawesi to 80 school children (40 from low land and 40 from highland altitudes) in order to compare the exposure intensity between the two areas. Method: Anti PGL-1 IgM antibody (ELISA) and PCR study to detect M.leprae in the nasal cavity were performed simultaneously from each person. Result: Seropositive cases were found in 23/40 children from low land compared to 16/40 children from high land, but statistically no significant difference (p>0.05). PCR positive for M.leprae in the nasal cavity only found in 1/40 children, both in low and high altitude. Conclusion: It is concluded that although the existence of M.leprae in nasal cavity is minimal, the intensity of exposure to this bacilli still high as indicated by serological study.
COMPARATIVE STUDY ON THE INTENSITY OF Mycobacterium leprae EXPOSURE BETWEEN HOUSEHOLD AND NONHOUSEHOLD CONTACT OF LEPROSY Arsyad, Yuniarti; Jifanti, Friska; Amiruddin, Muhammad Dali; Anwar, Anis Irawan; Adriaty, Dinar; Wahyuni, Ratna; Iswahyudi, Iswahyudi; Agusni, Indropo
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 1 (2012)
Publisher : Institute of Topical Disease

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Abstract

Leprosy stills a public health problem in West Sulawesi which has a Case Detection Rate (CDR) around 43.69/100.000 population. Household contacts of leprosy are a high risk group to be infected, due to droplet infection mode of transmission of the disease. A nose swab examination and serological study was conducted to detect exposure of M. leprae of people who live in leprosy endemic area. Detection of M. leprae in the nasal cavity will represent the exposure rate from outside and the measurement of specific antibody is represented the result of exposure to the immune system. Two group of inhabitants (30 household contacts of leprosy and 30 nonhousehold contacts) were involved in the study. They live in Banggae district, a leprosy endemic area of Majene Regency, West Sulawesi. Sixty nose swab samples and sixty capillary blood samples from the same invidividuals of the two groups were collected and sent to Leprosy laboratory of the Institute of Tropical Disease, Airlangga University Surabaya. A Polymerase Chain Reaction (PCR) was performed to the nose swab samples for detection of M. leprae. The blood samples were examined serologically to measure the level of anti PGL-1 antibody. PCR examination of nose swab samples showed 1/30 positive result in the household contact group and also 1/30 positive result in non-household contact of leprosy (statistically no significant difference, p > 0.05). Serological study showed higher sero-positive result in the household contact group (15/30 or 50%) compared to non-household contact (11/30 or 36%), but statistical calculation revealed no significant difference between the two groups (p > 0.05) on sero-positive results of leprosy. It is concluded that household and non-household contact in leprosy have the same risk to be affected by the disease. The term of household and non-household contact need to be redefined. The possible role of exposure from the environment was also discussed, especially from non-human resource of M. leprae.
DAPSONE RESISTANCE IN A Mycobacterium leprae ISOLATE WITH TWO POINT MUTATIONS IN folP GENE FROM A LEPROSY PATIENT Ardiaty, Dinar; Wahyuni, Ratna; Prakoeswa, Cita; Abdullah, Rasyidin; Agusni, Indropo; Izumi, Shinzo
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

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Abstract

Drug resistance in leprosy is important for Leprosy Control Program, since the WHO-Multidrug regiment (MDT) has been used for global treatment of leprosy for more than two decades already. A Dapsone resistance case in a Multibacillary (MB) leprosy case is reported. The patient was diagnosed and treated in Tajuddin Chalid Hospital Makassar, South Sulawesi. Previously he was treated in a health center at South Sulawesi and was given a treatment for one year, before referred to the hospital. The leprosy skin lesions are still active with erythematous skin lesions and thickened ear lobe. Bacteriological examination was positive for Acid Fast Bacilli, the Bacterial Index was 3+ and the Morphological Index was 1%. The specimens of M.leprae isolation was sent to the Institute of Tropical Disease Surabaya for drug resistance study. Using the Lp1-2 and Lp3-4 nested primers, PCR test was positive for M.leprae.Sequencing result for folP gene showed a double mutation at codon 53 (ACC / Threonin ) which become (AGG / Arginine). Simultaneous mutation at two nucleotides at one codon has never been reported in Indonesia before and this phenomenon is important for leprosy control policy.
Mycobacterium leprae BACILLEMIA IN BOTH TWINS, BUT ONLY MANIFEST AS LEPROSY IN ONE SIBLING Sukmawati, Netty; Agusni, Indropo; Listiawan, M. Yulianto; Prakoeswa, Cita Rosita S.; Adriaty, Dinar; Wahyuni, Ratna; Iswahyudi, Iswahyudi
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 7 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v5i7.1206

Abstract

Leprosy in twins is rarely reported. A 19 years-old male student, from Lamongan district, was diagnosed as Multibacillary (MB) leprosy in the Skin and STD Clinic of Dr. Soetomo General Hospital Surabaya. Multiple anesthetic skin lesions were found, but the bacteriologic examination was negative for Acid Fast Bacilli (AFB). Histopathology examination support the diagnosis of BL type of leprosy. His twin brother that has been lived together since born until present seems healthy without any complaints of skin lesions and have no signs of leprosy. When a serologic examination for leprosy was performed, a high anti PGL-1 antibody level was found in patient (IgM anti PGL-1 2937 and IgG anti PGL-1 3080 unit/ml) while his healthy twin brother showed only low level (IgM 745 and IgG 0 unit/ml). Interestingly when a PCR study was performed to detect M.leprae in the blood, both of them showed positive results. Using the TTC method, a genomic study of for M.leprae, it is revealed that both samples were identic ( 27x TTC repeats). According to patient’s history, he had a traffic accident and got a wound in the knee seven years ago, while the skin lesions seems started from this area around three years ago before it spread to other parts of the body. The patient was treated with Multi-drug therapy (MDT) while his sibling got a prophylactic treatment for leprosy. After 6 months of treatment, the leprosy skin lesions were diminished and the serologic anti PGL-1 has been decreased. His healthy brother also showed a decrease in anti PGL-1 level and no skin signs of leprosy.
Secreted Aspartyl Proteinase[SAP] Enzyme Profile on Vulvovaginalis Candidiasis Patients isolates Nugrahaeni, Diah; Agusni, Indropo; Ervianti, Evy
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 27, No 1 (2015): BIKKK APRIL 2015
Publisher : Faculty Of Medicine Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.891 KB) | DOI: 10.20473/bikkk.V27.1.2015.17-23

Abstract

Background: Vulvovaginalis candidiasis (VVC) is an infection caused by various types of Candida, themost is Candida albicans. Candida produces two main enzymes that play a role in virulence, the secreted aspartyl proteinase (SAP) enzyme and the phospholipase enzyme. SAP is most widely produced by Candida albicans,which is one of the main pathogenicfactor of Candida infection. SAP is often found in cases of recurrent candidiasis vulvovaginalis (RVVC) Purpose: To evaluatethe SAP activity produced by VVC patients isolates. Methods: Descriptive observational cross-sectional study was conducted on 21 VVC patients to determine the SAP enzyme levels in VVC patients in Sexually Transmitted Infection (STI) Division, Outpatient Clinic Of Dermatology and Venerology, Dr. Soetomo General Hospital, Surabaya.Vaginal smear was examined to determine levels of SAP and culture examination to determine the kind of candida spesies that causes VVC. Results: Total subject of study are 21 patients. Twelve of  21 patients had SAP > 20 mm in diameter, 7 patients with SAP < 20 mm in diameter, and 2 patients with negative results of SAP. Fifty patients due to Candida albicans, 6 patients caused by non-albicans. Nine patients were accompanied by diabetes mellitus (DM), 1 patients by genital affection, 2 patients by condylomata acuminata. Additionally obtained in 10 patients who had previously received treatment with the results diameter SAP > 20 mm and 7 patients with of SAP diameter < 20 mm, 2 patients showed negative results of SAP. Conclusions: SAP was mainly produced by Candida albicans. SAP became more active in VVC patients that accompanied by diabetes melitus, affectiogenitalis, condylomata acuminate, and also in some patients who have received prior antifungal therapy.Key words: secreted aspartyl proteinase, vulvovaginalis candidiasis, Candida albicans,
Reverse Transcription Polymerase Chain Reaction (RT-PCR) untuk Mendeteksi Viabilitas Mycobacterium leprae pada Pasien Kusta Tipe Multibasiler Pascapengobatan MDT-WHO Gayatri, Lunni; Listiawan, M. Yulianto; Agusni, Indropo
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 26, No 2 (2014): BIKKK AGUSTUS 2014
Publisher : Faculty Of Medicine Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.719 KB) | DOI: 10.20473/bikkk.V26.2.2014.1-6

Abstract

Latar belakang: Kusta masih menjadi masalah besar di Indonesia. Penatalaksanaan dengan MDT-WHO telah menjadi regimen standar untuk pasien kusta sejak lama. Pada pasien kusta tipe multibasiler (MB), indeks bakteriologis (IB) belum mencapai nilai nol, meskipun telah menyelesaikan terapi 12 regimen. Metode Reverse Transcription-Polymerase Chain Reaction (RT-PCR) memiliki sensitivitas tinggi untuk mendeteksi viabilitas M. leprae. Tujuan: Mengevaluasi viabilitas M. leprae dengan metode RT-PCR pada pasien kusta tipe MB yang telah menyelesaikan regimen MDT 12 di RSUD Dr. Soetomo Surabaya. Metode: 15 pasien kusta tipe MB yang telah menyelesaikan pengobatan MDT-WHO 12 regimen dengan IB positif dilakukan biopsi kulit untuk pemeriksaan RT-PCR. Hasil: Terdapat 13 pasien (86,7%) dengan viabilitas M. leprae positif sementara seluruh pasien tersebut (100%) memiliki Indeks Morfologis (IM) 0%. Dua belas pasien (80%) memiliki Indeks Bakteriologis 2+ dan 10 pasien diantaranya adalah pasien kusta tipe Lepromatous Leprosy (LL) polar. Delapan pasien yang memiliki viabilitas positif tidak mendapatkan kortikosteroid oral selama siklus MDT. Simpulan: Pasien kusta yang telah menyelesaikan terapi MDT dengan MI 0%, didapatkan viabilitas M. Leprae positif dengan metode RT-PCR. Spektrum klinis pasien yang ditentukan dari imunitas seluler nampaknya menjadi faktor terpenting dalam viabilitas M. leprae.Katakunci: viabilitas, M. leprae, kusta tipe multibasiler, RT-PCR.
Immunomodulators for a Variety of Viral infections of the Skin Wulan, I G A Kencana; Agusni, Indropo
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 27, No 1 (2015): BIKKK APRIL 2015
Publisher : Faculty Of Medicine Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.519 KB) | DOI: 10.20473/bikkk.V27.1.2015.63-69

Abstract

Background: In the last decade there has been inceasing prevalence of viral infections in the world, this led to increased efforts to find a variety of new drugs and vaccines against the virus that causes. Purpose: To disccuss the provision relating to the use of immunomodulators in viral infections. Review: Some diseases caused by viruses such as varicella, herpes zoster, herpes simplex, condyloma acuminata, molluscum contagiosum and Human immunodeficiency syndrome (HIV). Immunomodulator also known as biological response modifier, a wide variety of materials imunoaugmentor is either recombinant, synthetic, or natural medicines that returns an imbalance of the immune system that is used in immunotherapy. Immunotherapy is a treatment approach by restoring, enhancing, or suppresing the immune response. Immunomodulator administration on viral infections gave varying results. Conclusion: Immunomodulatory regimens on viral infections is an attractive therapeutic approach, because the side effects are often lighter than the side effects of drugs that have been there, in addition to the more rarely cause resistance to treatment of diseases caused by viral infection.Key word: viral infection, immunomodulator, immune response.
Profil Pasien Kusta Baru pada Anak Wulan, I G.A. Kencana; Agusni, Indropo; Rosita, Cita
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 26, No 2 (2014): BIKKK AGUSTUS 2014
Publisher : Faculty Of Medicine Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.047 KB) | DOI: 10.20473/bikkk.V26.2.2014.1-6

Abstract

Latar belakang: Kusta hingga saat ini masih menjadi masalah kesehatan di Indonesia. Kusta pada anak merupakan indeks epidemiologis untuk menentukan transmisi penyakit serta sebagai salah satu indikator dalam keberhasilan program pemberantasan penyakit kusta nasional. Tujuan: Mengevaluasi profil pasien kusta pada anak. Metode: Penelitian retrospektif terhadap semua kasus kusta baru pada anak (0-14 tahun) yang datang ke Unit Rawat Jalan (URJ) Kesehatan Kulit dan Kelamin RSUD Dr.Soetomo Surabaya mulai Januari 2009 sampai Desember 2011. Hasil: Didapatkan 37 pasien anak (5,5%) dari 677 kasus kusta baru. Kasus paling banyak (70,3%) terdapat pada kelompok usia 10-14 tahun. Laki-laki lebih banyak daripada perempuan. Berdasarkan klasifikasi WHO, sebanyak 29 kasus (78,4%) adalah kusta multibasiler (MB) dan 8 kasus (21,6%) adalah kusta pausibasiler (PB). Tipe terbanyak adalah kusta tipe BB (59,5%) pada kelompok MB dan diikuti tipe TT (16,2%) pada kelompok PB. Seluruh kasus mendapat pengobatan Multidrug Therapy (MDT) sesuai panduan WHO. Kontak serumah didapatkan 68,7% kasus. Simpulan: Ditemukan 37 kasus kusta baru pada anak di RSUD Dr. Soetomo Surabaya selama tiga tahun, terutama pada kelompok umur 10-14 tahun, dengan kasus terbanyak berupa kusta MB tipe BB. Kontak serumah masih memegang peranan yang penting pada transmisi kusta.Kata kunci: kusta, anak-anak, retrospektif.
Oral Corticosteroid Therapy in Leprosys new patients with Type 2 Reaction Listiyawati, Irma Tarida; Sawitri, Sawitri; Agusni, Indropo; Prakoeswa, Cita Rosita Sigit
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 27, No 1 (2015): BIKKK APRIL 2015
Publisher : Faculty Of Medicine Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.721 KB) | DOI: 10.20473/bikkk.V27.1.2015.48-54

Abstract

Background: Leprosy type 2 reactions is an acute inflammatory episode in lepromatous leprosy patient that ascociated with deposition of immune complexes. Standard regiment for severe reaction is corticosteroid. Almost Leprosy type 2 reactions patients become chronic and recurrent so it implicates to more complications if it was not managed well. Purpose: To determine the distribution of type 2 reactions patients which treated with oral corticosteroid in leprosy division of dermatovenereology outpatient clinic of Dr. Soetomo General Hospital. Methods: Retrospective study using new patients medical record during the period of January 1 2009 until December 31 2013 and the observation continued until December 31 2013. Results: The total type 2 reactions patients with oral corticosteroid was 112 patients, mostly were male aged between 25-64 years and has the Lepromatous Leprosy (LL) type (33%), the reactions happened after RFT  (43.8%), 65.2% had metil prednisolon medication, the initial equivalent dose was 30 mg in 32.1% patient. 21.4% patient had more than one cycle  of corticosteroid therapy and 8.9% patient had the steroid side effect. Conclusion: Leprosys type 2 reactions tend to be chronic and recurrent and the impact were patients got longterm continuously corticosteroid medication, so the patients need to be monitored including side effects.Key words: leprosys type 2 reaction, oral corticosteroid, side effects.
Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara Adriaty, Dinar; Wahyuni, Ratna; Iswahyudi, Iswahyudi; Aksono, Bimo; Koesbardiati, Toetik; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.2.3.%x

Abstract

The existence of leprosy or kusta or Morbus Hansen or Hansens disease has been known for years, including in Indonesia. Starting from the discovery of Mycobacterium leprae isolates from ancient bone (about 1.000 years B.C), the archaeological excavations results in East Nusa Tenggara, interesting questions arise about how the development of leprosy in eastern Indonesia is. Biology molecular study would become a powerful tool to investigate the presence of leprosy bacillary whether there are similarities between the genomes of M. leprae isolates in the primeval and the present. PCR examinations were performed on mandibular bone fragments from ancient human who lived 1000 years B.C. discovered in archaeological surveys on the island of Lembata and three leprosy patients from East Nusa Tenggara. The DNA extraction was performed using a kit from Qiagen products and its TTC repeating pattern was seen with the method of direct sequencing. It turned out that the TTC profile obtained from samples of archaeological was as many as 13 copies, while the repetition of TTC in three samples of leprosy patients were 15, 17 and 26 copies. The different number of TTC repetition shows the different isolates of M. leprae between in the ancient times and the present. Further studies are needed to verify the differences in the genome that occur, for example from the study of SNPs (single nucleotide polymorphisms).
Co-Authors A. M. Adam, A. M. Afif Nurul Hidayati Afif Nurul Hidayati, Afif Nurul Agustina Tri Pujiastuti Anggraeni, Sylvia Anis Irawan Anwar Astindari Astindari, Astindari Aulia, Syifa Bambang Wirjatmadi Bimo Aksono Bimo Aksono Cita Prakoeswa, Cita Cita Rosita CITA ROSITA S. PRAKOESWA CITA ROSITA S. PRAKOESWA Cita Rosita Sigit Prakoeswa Cita Rosita, Cita Diah Mira I Diah Nugrahaeni Diah Nugrahaeni, Diah DINAR ADRIATY Dinar Ardiaty, Dinar Ditta Harnindya Dwi Murtiastutik Endang Retnowati Evy Ervianti Fanny Iskandar, Fanny Farida Tabri Febrina Dewi Pratiwi Friska Jifanti Halik Wijaya I G A Kencana Wulan I G A Kencana Wulan, I G A Kencana I G.A. Kencana Wulan I G.A. Kencana Wulan, I G.A. Kencana Icha Aisyah Indah Sari LD Irawan Yusuf Irma Tarida Listiyawati Irma Tarida Listiyawati, Irma Tarida ISWAHYUDI ISWAHYUDI Iswahyudi Iswahyudi Kinanti Prabawaningrum Kristina Sihaloho Linda Astari, Linda Lunni Gayatri Lunni Gayatri, Lunni Lydiawati, Eva M. dali amiruddin, M. dali M. Yulianto Listiawan Mansur Arif, Mansur Maylita Sari, Maylita Meita Ardini Pratamasari, Meita Ardini Mohammad Z. Rahfiludin Muhammad Dali Amiruddin Netty Sukmawati, Netty NI PUTU SUSARI Nuswantoro, Djohar Prakoeswa, Cita Rosita rachmah diana putri, rachmah diana Rachmawati Rachmawati Rahmadewi Rahmadewi Rahmatari, Bandaru Rasyidin Abdullah Ratana, Heng Ratna Wahyuni RATNA WAHYUNI Rinasari, Umi Safruddin Adam, Safruddin Safruddin Amin Santoso, Rachmat Sawitri Sawitri Sawitri Sawitri Sepling Paling SEPTIANA SEPTIANA Sheilaadji, Maria Ulfa Shinzo Izumi SHINZO IZUMI SHINZO IZUMI SHINZO IZUMI Sirithida, Chukmol Siti Kurniawati Tasalina Yohana Parameswari Gustam Timurleng Tonang Mataallo, Timurleng Tonang Toetik Koesbardiati Vannda, Sou Vortey, Hak Willy Sandhika Yoes P. Dahlan Yuniarti Arsyad, Yuniarti Yuri Widia, Yuri Yusuf Wibisono