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Akurasi Pemeriksaan Kato-Katz dan Mini-Flotac Dalam Diagnosis Kecacingan pada Feses Segar dan Feses Awetan Iqbal, Maulana; Triana, Dessy; Rizqoh, Debie; Gunasari, Lala Foresta Valentine; Umar, Liya Agustin
Jurnal Kedokteran dan Kesehatan Vol 19, No 1 (2023): JURNAL KEDOKTERAN DAN KESEHATAN
Publisher : Faculty of Public Health, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jkk.19.1.74-82

Abstract

Soil transmitted helminth (STH) penyebab infeksi kecacingan dengan insiden 24% pada populasi di dunia. Diagnosis infeksi STH dapat dilakukan dengan pemeriksaan feses secara mikroskopis dengan metode Kato-Katz sebagai baku emas dan metode Mini-Flotac. Tujuan penelitian menganalisis akurasi hasil pemeriksaan feses menggunakan metode Kato-Katz dan metode Mini-Flotac pada feses awetan formalin 10%. Rancangan penelitian menggunakan studi observasional dengan uji diagnostik. Subjek pada penelitian berjumlah 140 sampel feses awetan formalin 10% selama 13 bulan. Pemeriksaan spesimen feses awetan menggunakan metode Kato-Katz dan Mini-Flotac serta diamati dengan menggunakan mikroskop cahaya. Data analisis dilakukan dengan uji T. Hasil penelitian menunjukkan sebagian besar sampel feses awetan terinfeksi Ascaris lumbricoides dengan metode Kato-Katz (34,28%) dan Mini-Flotac (41,42%), dan infeksi Trichuris trichiura dengan metode Kato-Katz (15,71%) dan Mini-Flotac (22,85%). Tidak terdapat perbedaan yang signifikan antara metode Kato-Katz dan Mini-Flotac dalam mendeteksi infeksi Ascaris lumbricoides (p=0.96) dan Trichuris trichiura (p=0,76). Metode Mini-Flotac dapat menjadi alternatif dalam diagnosis infeksi STH.
Larvae-Free Rate Aedes sp: The Effect of Temperature, Humidity and Rainfall in Bengkulu City, Indonesia Gunasari, Lala Foresta Valentine; Aladdawiyah, Zahara Septika; Suwarsono, Suwarsono; Triana, Dessy
Journal of Public Health for Tropical and Coastal Region Vol 7, No 3 (2024): Journal of Public Health for Tropical and Coastal Region
Publisher : Faculty of Public Health, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jphtcr.v7i3.24324

Abstract

Abstract Background: Bengkulu's tropical climate is very suitable for Ae. aegypti and Ae. albopictus mosquito breeding. Temperature, humidity and rainfall affect mosquito breeding and dengue virus proliferation in mosquitoes, which in turn had an effect on the dengue infections. Dengue prevention strategy currently focuses on vector control with the Larvae Free Rate (LFR) parameter. This study aimed to determine environmental factors affecting the LFR in 2022 in Bengkulu City, Indonesia.Methods: This research used a cross-sectional study design with time series analysis. Time series was used to analyze and interpret sequential data points collected over  the interval of time. Secondary data about LFR was obtained from the Bengkulu City Health Service, and climate variables from the Climatology Station of the Bengkulu Meteorology, Climatology, and Geophysics Agency. Data analysis was conducted through simple linear regressions.Results: The average of LFR in Bengkulu City in 2022 was 78.13% (below the national target), with the highest in December (87.85%) and the lowest in May (68.53%). The average temperature, humidity, and rainfall were 26.88°C (moderate), 83.5% (moderate), and 328.96% (high), respectively. The simple linear regression analysis showed that the effect of temperature, humidity, and rainfall was significant to LFR with the respective p-values of 0.010; 0.612, and 0.173).Conclusion: Temperature influenced the LFR by 58,5% but humidity and rainfall have no effect on LFR of Aedes spp. in Bengkulu City in 2022. High temperatures will increase the risk of transmission of dengue virus infection by 3-5%. 
Differences in Before and After Temephos Intervention in Bengkulu City: Larvae Free Rate of Aedes spp. Gunasari, Lala Foresta Valentine; Destilyana, Rizqita; Massardi, Nikki Aldi; Triana, Dessy
Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan Vol 22, No 1 (2025): March 2025
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/presipitasi.v22i1.157-167

Abstract

One of the strategies to reduce dengue transmission is to control the population of vectors, such as larval eradication. The use of temephos larvicide (Abate®) is the most widely carried out in the community. An indicator of the success of the larval eradication program is the Larvae Free Rate (LFR) assessment. This study aimed to determine the difference between LFR of Aedes spp. before and after temephos intervention in Bengkulu City. This study is experimental research with one group pre-test and post-test design. The population of this study was all houses in Bengkulu City with a total sample of 670 houses. Sampling was carried out using cluster random sampling techniques in 67 urban villages in Bengkulu City. This research was carried out by conducting a survey of larvae before and after temephos intervention in containers. Sixty-seven urban-villages, in 44 (65,67%) urban-villages was found an increase of LFR after temephos intervention. The average LFR before temephos intervention was 67.61%, while after temephos intervention increased to 82.98%, there was a significant difference between LFR of Aedes spp. before and after temephos intervention in Bengkulu City (p=0.001). Temephos intervention is effective to increase LFR of Aedes spp. in Bengkulu City.
Penyuluhan dan Pelatihan tentang Keselamatan dan Kesehatan Kerja pada Petugas Pengangkut Sampah di Dinas Lingkungan Hidup Kota Bengkulu Gunasari, Lala Foresta Valentine; Oktoviani; Anggraini, Rizkianti; Agustriawan, Deni; Indiarma, Verani; Triana, Dessy
DHARMA RAFLESIA Vol 23 No 1 (2025): JUNI (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v23i1.37470

Abstract

Petugas pengangkut sampah beresiko tinggi mengalami penyakit akibat kerja, salah satunya ialah infeksi soil-transmitted helminths (STH) karena pekerjaannya sangat memungkinkan mereka untuk kontak dengan telur dan larva cacing yang ada di tanah atau sampah. Penelitian yang dilakukan pada 84 petugas pengangkut sampah di Dinas Lingkungan Hidup Kota Bengkulu tahun 2022 lalu menunjukkan bahwa 46 orang (54,8%) di antaranya mengalami infeksi STH. Kesimpulan dari penelitian tersebut ialah terdapat hubungan antara personal hygiene yang buruk dan tidak menggunakan Alat Pelindung Diri (APD) yang sesuai standar dengan infeksi STH. Sebagai tindak lanjut, dilakukan kegiatan Pengabdian kepada Masyarakat berupa penyuluhan dan pelatihan untuk meningkatkan kesadaran tentang keselamatan dan kesehatan kerja (K3) pada 92 petugas pengangkut sampah di Dinas Lingkungan Hidup Kota Bengkulu. Kegiatan ini terbukti dapat meningkatkan pengetahuan peserta mengenai bahaya kesehatan yang dapat ditimbulkan akibat pekerjaan yang kontak dengan sampah, pentingnya menggunakan APD saat bekerja, cara penggunaan APD yang sesuai standar, cara menjaga personal hygiene, dan perlunya mengkonsumsi obat cacing sebagai upaya eliminasi dan pencegahan infeksi STH.
REKOMENDASI STRATEGI PENGENDALIAN DENGUE DI KOTA BENGKULU TAHUN 2023-2025: FOCUS GROUP DISCUSSION (FGD) Triana, Dessy; Gunasari, Lala Foresta Valentine; Martini, Martini; Suwondo, Ari; Sofro, Muchlis Achsan Udji
DHARMA RAFLESIA Vol 23 No 1 (2025): JUNI (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v23i1.39717

Abstract

Dengue is a global health problem. There has been an increase in cases of more than 15 times over the last two decades. The causes of the rise in the number of cases and the increase in infected areas are very complex and multifactorial, including viral, vector, environmental, and human factors. A comprehensive prevention strategy is needed that includes all component factors that influence dengue disease to predict the incidence of the disease. This activity aimed to implement effective dengue control in Bengkulu City to reduce the number of dengue cases. The activity partners were the Bengkulu City Health Service, Bengkulu Provincial Health Service, Community Health Centers in Bengkulu City, and surveillance officers. The method of this activity was to focus group discussion to formulate a dengue control strategy in Bengkulu City for 2023-2025.  The dengue control strategy that has been formulated can be implemented in the work area of ​​the community health center to reduce the number of dengue cases.
Accuracy of the Kato-Katz and direct examination in the diagnosis of worms in preserved feces Gunasari, Lala Foresta Valentine; Triana, Dessy; Reyudo Suringgar, Sadid
BKM Public Health and Community Medicine PHS8 Accepted Abstracts
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Purposes: Soil-transmitted helminth (STH) causes helminthiasis with an incidence of 24% in the world's population. Children aged 2-14 years are the population most susceptible to helminthiasis. In Indonesia, there are 60.4 million children who have worms. Worms can cause delays in the growth and development of children. Worms can be diagnosed through a qualitative (direct examination) and quantitative (Kato-Katz) stool examination. This study aimed to analyze the accuracy of stool examination results using the Kato-Katz method and the Direct Examination method on 10% formalin-preserved feces. Methods: The research design used an observational study with diagnostic tests. The research subject amounted to 140 samples of 10% formalin preserved feces for 13 months. Examination of preserved stool specimens used the Kato-Katz and Direct Examination methods and was observed using a light microscope. Results: The results showed that 45,71% were infected with STH using the Kato-Katz method and direct examination of 35.71%. The accuracy test showed that the sensitivity and specificity values of the Kato-Katz method were 86.79% and 79.31%, respectively. The direct examination was obtained by 64.15% and 81.60%. Conclusions:The Kato-Katz method is more sensitive than the direct examination method in diagnosing helminthiasis in preserved feces, but the direct examination method has a higher specificity value than the Kato-Katz method.
Accuracy test of the Kato-Katz and direct examination method for diagnosing helminthiasis using preserved stool Gunasari, Lala Foresta Valentine; Triana, Dessy; Suringgar, Sadid Reyudo
BKM Public Health and Community Medicine Vol 38 No 01 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bkm.v38i01.3535

Abstract

Purposes: More than 1.5 billion people, or a quarter of the world's popula-:on, are infected by soil-transmi>ed helminth (STH). Children aged 2-14 years are the most suscep:ble to helminthiasis. In Indonesia, there are 60.4 million children infected with STH, with an average prevalence rate of 31.8%. Helminthiasis can cause growth and development disorders in children. The diagnosis of helminthiasis can be made by examining stool using qualitative (direct examination) and quantitative (Kato-Katz) methods. This study aimed to determine the accuracy of both methods in diagnosing helminthiasis using preserved stool specimens. Methods: This research design was an observational study with a cross-sec-:onal approach. A total of 140 stool samples preserved using 10% formalin for 13 months were examined with the Kato-Katz and direct examination methods using a light microscope. Results: Among the 140 stool samples examined, 64 samples (45.71%) were found positive for STH using the Kato-Katz method, while with the direct examination method, 50 samples (35.71%) were positive for STH. The accuracy test showed that the sensitivity and specificity values of the Kato Katz method were 86.79% and 79.31%, while the direct examination method was 64.15% and 81.60%, respectively. Conclusions: The Kato-Katz method was more sensitive than the direct examination method in diagnosing helminthiasis in preserved stool, but the direct examination method had a higher specificity value than the Kato-Katz method. In addition, the accuracy of examination of fresh and preserved stool specimens in diagnosing helminthiasis was not significantly different.