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Characterization And Acute Toxicity Test Of Black Garlic Ethanol Exctract Based On OECD Ellen Stephanie Rumaseuw; Yoppi Iskandar; Eli Halimah; Ade Zuhrotun
Interest : Jurnal Ilmu Kesehatan INTEREST: Jurnal Ilmu Kesehatan Volume 10 Number 2 Year 2021
Publisher : Poltekkes Kemenkes Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37341/interest.v0i0.379

Background: Garlic (Allium sativum, L) was used empirically by the ancestors as a useful plant for hypercholesterolemia. The processed garlic product, namely black garlic, has gone through an optimal heating process at a temperature of 70oC for 21 days. Black garlic can become a standardized herb if it has been through characterization and toxicity tests. Methods: The characterization test is known by measuring several general standard parameters of Indonesian extracts. Acute toxicity test was carried out by dividing 5 treatment groups namely negative control, group I (5 mg/kg BW), group II (50 mg/kg BW), group III (300 mg/kg BW), group IV (2000 mg/kg BW). Results: The results showed that the standardization of black garlic extract has a drying shrinkage content of 7.24%, a water content of 8.8%, a total ash content of 4.79%, an acid insoluble ash content of 1.52%, a water soluble extract content of 7.47% and an ethanol soluble extract content of 9.3% which is still into the Indonesian herbal pharmacopoeia standard and the administration of black garlic ethanol extract caused mild toxic symptoms during the acute toxicity test and obtained LD50 values > 2000 mg/kg BW. Conclusion: Characterization of black garlic extract according to standardization parameters of Indonesian plant extracts and administration of ethanolic extract of black garlic has an LD50 value > 2000 mg/kg BW which is included in category 5 in the OECD.

UJI KADAR AIR JAMU SERBUK PENURUN BERAT BADAN YANG BEREDAR DI E-MARKETPLACE Ellen Stephanie Rumaseuw; Febriaryanti Aritonang
Jurnal Kesehatan Vol. 9 No. 2 (2021): Oktober : Health Journal “Love That Renewed”
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

Anak muda saat ini lebih menyukai menurunkan berat badan dengan cara instan misalnya dengan mengkonsumsi jamu serbuk yang dijual di pasaran daripada berolahraga rutin dan menjaga pola makan. Jamu serbuk penurun berat badan mudah didapatkan dari layanan e-marketplace yang dapat dibeli secara online. Pemerintah sendiri mengeluarkan peraturan Kepala BPOM Nomor 32 Tahun 2019 tentang Persyaratan Keamanan dan Mutu Obat Tradisional yang menyatakan bahwa setiap obat tradisional harus memiliki batas nilai kadar air ≤ 10%. Pengujian kadar air dilakukan pada produk jamu serbuk karena jumlah kadar air yang melebihi nilai persyaratan maksimum menyebabkan kerusakan pada jamu serbuk oleh adanya fermentasi jamur dan adanya mikroba yang mengakibatkan daya tahan jamu serbuk dalam penyimpanan menurun. Tujuan penelitin ini bertujuan mengetahui nilai kadar air produk jamu serbuk penurun berat badan yang beredar di e-marketplace sesuai dengan ketentuan BPOM. Metode penetapan kadar air menggunakan gravimetri pada 9 produk jamu serbuk penurun berat badan. Berdasarkan hasil penelitian diperoleh nilai rerata kadar air lima sampel jamu bermerek sebesar 0,33%; 3,78%;6,86%; 7,85%; dan 5,39%. Sedangkan nilai rerata kadar air empat sampel jamu tidak bermerek sebesar 5,71%; 4,10%; 5,52% dan 0,53%. Kesimpulan penelitian ini bahwa 9 produk jamu serbuk penurun berat badan yang beredar di e-marketplace memenuhi persyaratan BPOM.

Hematological Parameters in Subchronic Toxicity Test of Black Garlic Ethanol Extract in Rats Ellen Stephanie Rumaseuw; Yoppi Iskandar; Eli Halimah
Journal of Fundamental and Applied Pharmaceutical Science Vol 2, No 2 (2022): February
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/jfaps.v2i2.13755

The community has used black garlic since ancient times for hypercholesterolemic. Until now, people still consume both raw and cooked black garlic. Black garlic is included in processed garlic products. People on the Asian continent have used black garlic for the past 10 years. This study aims to determine the sub-chronic toxic effect of black garlic ethanol extract on male and female white rats using hematological parameters consisting of hematocrit, hemoglobin, erythrocytes, leukocytes, platelets, MCV, MCH, and MCHC. This research method was a completely randomized design with the administration of ethanolic extract of black garlic to rats in 5 treatment groups, including a negative control group, a dose group of 1000 mg/kg BW, a dose group of 2000 mg/kg BW, a negative control satellite group and a satellite group with a dose of 2000 mg/kg BW. Data were analyzed statistically using one-way ANOVA with a 95% confidence level and SPSS version 20. The study results of black garlic ethanol extract showed no significant effect or were at normal levels on hematological parameters. Therefore, it can be concluded that the ethanolic extract of black garlic is safe for human use as a treatment for hypercholesterolemia.

THE WATER CONTENT TEST ON UNREGISTERED BRAND SLIMMING JAMU CIRCULATION IN CIMAHI CITY Ellen Stephanie Rumaseuw; Ancilla Dinda Kartika Candra
Jurnal Kesehatan Vol. 10 No. 2 (2022): Oktober: Health Journal "Love That Renews"
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

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Based on rules of National Agency of Drug and Food Control No. 32 of 2019 concerning the Safety and Quality Requirements of Traditional Medicines, it is stated that every traditional medicine must have a water content value of 10%. Excessive water content in a product will result in a decrease in the quality of traditional medicinal preparations. This study was conducted with the aim of determine the percentage of the water content of unregistered branded slimming herbal products circulating in Cimahi City by the provisions of water content requirements based on BPOM. This research is quantitative research using experimental methods. Determination of water content in this study using the gravimetric method, with the heating method at a temperature of 105°C at a certain time. Based on the results of the study, it was obtained that the average value of water content in the six herbal medicine samples, namely samples A, B, C, D, E, F, respectively, was 10.5%; 13.55%; 10.65%; 11.58%; 11.01% and 8.48%. So that the value of the water content of branded slimming herbs is not registered in Cimahi City, only one of the six samples studied has met the requirements set by BPOM is F sample.

DETERMINATION OF WATER SOLUBLE AND ETHANOL SOLUBLE CONTENT OF MATOA (Pometia pinnata J. R. Forst & G. Forst) LEAVES Ellen Stephanie Rumaseuw; Febriana Imelda
Jurnal Kesehatan Vol. 11 No. 1 (2023): April : Health Journal "Love That Renews"
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

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The matoa plant (Pometia pinnata J.R. & G.Forst) has been known to have many pharmacological effects and is used as traditional remedy. Matoa plants are recognized to contain secondary metabolites that have potential as medicine. One of the parts of matoa leaves that is traditonally as antibacterial, diuretic, analgesic and others. The quality of raw materials for traditional medicine is identified by the standardization. The standardization of implicial quality parameters is one of the specific parameters that test the water soluble implic and the ethanol soluble essence content. The reason of this observe become to determine water soluble essence and ethanol soluble essence content material on matoa leaf implicial. The take a look at uses an experimental approach. Matoa leaves were received through macerate extraction method the use of 2 types of solvents, specifically water solvent and 96% ethanol solvent. The effects showed that the water soluble essence and ethanol soluble essence values had been 15,81% and 27,72%.

IDENTIFICATION OF MEFENAMATIC ACID ON HERBAL PRODUCTS IN CIMAHI AREA WITH THIN LAYER Chromatography (TLC) METHOD Ellen Stephanie Rumaseuw; Krisna Pratama Putra Santoso
Jurnal Kesehatan Vol. 11 No. 2 (2023): Oktober : Health Journal "Love That Renews"
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

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Indonesian people use traditional medicines freely for alternative and complementary medicine. One of the traditional medicines consumed by the community is the herbal medicine for sore throat. Herbs that have good quality and pass safety tests can be widely produced by the pharmaceutical industry. Therefore, researchers want to conduct research on 5 herbal medicine products circulating in the Cimahi area. This study was conducted with the aim of knowing the herbal medicine for aches and paint circulating in the Cimahi area which contains the chemical drug mefenamic acid. The method used is thin layer chromatography with mobile phase : chloroform : ethyl acetate : glacial acetic acid ( 75 : 25 : 1 ) and silica gel Gf 254 as stationary phase. The results of this study prove that form the 5 product sample, there are 3 product sample that are suspected to contain mefenamic acid namely code A, C and E sample with the Rf values of 0,58, 0,55 and 0,78 respectively. Mefenamic acid has a standard Rf of 0,66 so that the 3 samples of herbal products are recommended not to be circulated and consumed by the public because they are not in accordance with the regulations of law no. 36 of 2009 concering health and regulation of the minister of health of the republic of Indonesia no. 007 of 2012 where both regulation prohibit the presence of chemical in traditional medicine.

Uji Toksisitas Akut Ekstrak Etanol Bawang Hitam Rumaseuw, Ellen Stephanie; Iskandar, Yoppi; Halimah, Eli
Indonesian Journal of Biological Pharmacy Vol 2, No 1 (2022): IJBP (April)
Publisher : Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijbp.v2i1.37973

Negara Indonesia memiliki keberanekaragaman hayati jenis tanaman yang tersebar di seluruh wilayah. Bawang hitam merupakan produk olahan bawang putih yang telah melalui proses pemanasan optimal pada suhu 70oC selama 21 hari. Bawang hitam dapat menjadi herbal terstandar bila telah melalui uji kualitas dan keamanan. Tujuan penelitian ini yaitu mengetahui tingkat keamanan ekstrak etanol bawang hitam. Uji keamanan dengan cara uji toksisitas akut dengan 5 kelompok perlakuan yaitu kontrol negatif, kelompok I (5 mg/kg BB), kelompok II (50 mg/kg BB), Kelompok III (300 mg/kg BB), kelompok IV (2000 mg/kg BB). Penelitian yang diperoleh menyatakan bahwa parameter standar bawang hitam memenuhi parameter standar umum tumbuhan Indonesia. Pemberian ektrak etanol bawang hitam menimbulkan gejala-gejala toksik ringan selama pengamatan pada uji toksisitas akut dan memperoleh nilai LD50 > 2000 mg/kg BB yang termasuk pada kategori 4 pada OECD Guidelines for The Testing of Chemicals.

Innovation In The Development Of Black Garlic As An Ingredient In Coffee Ellen Stephanie Rumaseuw; Roma Ave Maria; Monica Saptaningsih
JURNAL FARMASI GALENIKA Vol 11 No 1 (2024): Jurnal Farmasi Galenika Vol 11 No 1
Publisher : Universitas Bhakti Kencana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70410/jfg.v11i1.327

The phenomenon of coffee drinking influences thought patterns, health, and behavior in today's society. The joy of coffee comes from its taste and efficacy. Innovation the raw material for coffee in this study is black garlic with ginger powder added. Black garlic is a processed garlic product that contains an organic sulfur compound called S-allylcysteine sulfoxide, which has antioxidant properties. Another benefit of black garlic is that it can increase your body's stamina/vitality. The addition of ginger powder, which acts synergistically as an antioxidant, also contributes to the flavor of the coffee. This is expected to increase the creation of types of coffee-making ingredients that can improve taste and enhance functional benefits for human health. This study aims to determine the correct formulation of black garlic coffee with added ginger powder to have a positive impact on health. Chemical testing is performed by analyzing components such as: Antioxidants and proximate analysis, polyphenols; have potential applications in food and health products, as well as among herbal ingredients that maintain the body's stamina and vitality. The test process of sample analysis is carried out in the laboratory and the data is analyzed descriptively by presenting tables and plots of the obtained results in relation to the product potential. The technology used to develop Black Garlic Coffee is based on the processing of garlic, which is fermented for a period of time at high temperatures to produce new chemical components and recipes. This reserach have results that antioxidant content and detailed analysis of black garlic coffee could make it a globally competitive and local food that boosts stamina and vitality. This is supported by its antioxidant capacity and IC50% content of 8406.0677 ppm, low fat content of 0.20% and carbohydrate content of 37.03%. This is also supported by the moisture content of 53.02%; Ash content 1.53%; Protein 8.23%. One of the antioxidants present in black garlic coffee is total polyphenols, which have been shown containing 0.21% FeCl3 and Folin-Ciocalteu

Identifikasi Makroskopis, Mikroskopis, Dan Skrining Fitokimia Bunga Telang (Clitoria ternatea, L.) Varian Biru Dan Ungu Muda Lingir Huar, Salestina; Rumaseuw, Ellen Stephanie
JURNAL FARMASI GALENIKA Vol 12 No 1 (2025): Jurnal Farmasi Galenika
Publisher : Universitas Bhakti Kencana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70410/jfg.v12i1.386

Butterfly pea flower (Clitoria ternatea, L.) is a medicinal plant known for its pharmacological properties due to the presence of various bioactive compounds. This study aims to identify the macroscopic, microscopic, and phytochemical characteristics of the blue and light purple variants of butterfly pea flowers cultivated in Cipangeran Village, Saguling District, West Bandung Regency. The research employed a descriptive qualitative method with an experimental approach. Macroscopic identification was conducted by observing the color, shape, odor, and texture of the flowers, while microscopic observation was performed using powdered simplicia preparations under a microscope finding indentifier fragmen. Phytochemical screening was conducted using specific reagents to detect the presence of secondary metabolites such as alkaloids, flavonoids, saponins, steroids/triterpenoids, and tannins. The results showed that both flower variants have morphological features typical of the Fabaceae family, with distinct color gradients and varied textures. Microscopic analysis revealed identifying fragments such as sclerenchyma, pollen grains, vascular bundles, covering trichomes, collenchyma, and idioblasts containing oil cells. Phytochemical screening indicated the presence of flavonoids, steroids/triterpenoids, tannins, and alkaloids in both variants. Saponins were detected only in the blue variant. These findings suggest that both blue and light purple butterfly pea flower variants have potential as raw materials for herbal health products.

Testing of Non-Specific Parameters of Butterfly Pea Flower Cultivation from Cipangeran Village, Saguling District, West Bandung Regency Rumaseuw, Ellen Stephanie; Saptiningsih, Monica
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.10102

The blue butterfly pea flower (Clitoria ternatea) is a medicinal plant rich in bioactive compounds, including anthocyanins and flavonoids, with health benefits and potential in the food and cosmetics industries. There has been no scientific study on the non-specific parameters of cultivated butterfly pea flowers and their simple quality related to the environmental conditions in the village. Standardization of non-specific parameters, such as moisture content, ash content, and microbial contamination, is crucial to ensure the quality and safety of butterfly pea-based products. This research aims to analyze the non-specific parameters of butterfly pea in support of developing quality standards suitable for the pharmaceutical and cosmetic industries. The research method uses experimental techniques, starting with sample collection, laboratory analysis, and data analysis. The results of the water-soluble ash test, 99.63% indicate contamination by inorganic compounds that dissolve in water, while the acid-insoluble ash content of 0.09% suggests minimal contamination by silica. The water-soluble extract content of 54.98% and the ethanol-soluble extract content of 99.98% indicate the solubility of compounds in solvents, which are important for the extraction and formulation of traditional medicine products or herbal products that use water and ethanol as solvents. In conclusion, the results of non-specific parameter tests on the blue butterfly pea flower simplicia from Cipangeran Village indicate that the simplicia has excellent physicochemical qualities.

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