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All Journal Prosiding Omni-Akuatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed Sciscitatio : Journal for Biological Science Berkala Penelitian Hayati Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Aris Mumpuni
Fakultas Biologi – UNSOED, Purwokerto
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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EKPLORASI JAMUR KUPING (Auricularia spp.) INDIGENOUS KABUPATEN BANYUMAS Mumpuni, Aris; , Purnomowati; Risyanto, Slamet
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Wilayah Kabupaten Banyumas mempunyai kisaran kondisi klimat beragam, mengikuti kisaran ketinggian tempat yang cukup luas serta tersedianya beragam jenis tumbuhan penghasil komponen lignoselulosa sebagai substrat berbagai jenis jamur lignoselulosik. Kondisi tersebut mendukung diperolehnya keragaman jenis jamur termasuk jamur kuping indigenous yang perlu dieksplorasi untuk berbagai kepentingan, termasuk untuk mendukung upaya pengembangan produksinya. Penelitian yang dilaksanakan dengan metode survei ini secara umum mendapatkan ada 2 (dua) macam isolat jamur kuping dengan ciri morfologi tubuh buah yaitu berwarna merah coklat tua dan merah coklat muda dengan tekstur yang relatip sama yaitu berlendir tetapi dengan kecepatan pertumbuhan koloni miselia dan kualitas pertumbuhan miselia yang berbeda. Jamur kuping dengan warna tubuh buah merah coklat tua pada umumnya pertumbuhan miselianya lebih baik daripada jamur kuping dengan warna tubuh muah merah coklat muda.
Growth and Protein Content Establishment of Pleurotus ostreatus on Liquid and Solid Medium Mumpuni, Aris; Ekowati, Nuraeni; Purnomowati, Purnomowati; Purwati, Endang Sri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11660

Abstract

Pleurotus ostreatus cultivation is performed using solid medium to harvest fruit body and using liquid medium to harvest mycelia in submerged culture. Modifying nutrients in the medium to increase protein content of the fruitbody and mycelia can be done through addition of nitrogen-containing materials. This study aims to determine: the appropriate composition of the liquid medium for high mycelial growth and protein content; and the exact composition of the solid medium to obtain high fruitbody product and protein content. The method was experimental with completely randomized design (CRD). The treatments were incubation of P. ostreatus on three types of liquid medium and four types of solid medium. The results showed that the optimal liquid medium composition for mycelial growth was Liquid Fermentation Medium 1 (FC1) with 10% corn fluor, and the highest protein content was in Liquid Fermentation Medium 2 (FC2 = 29.76%). While the optimal solid medium composition for fruitbody production was the medium with 3% corn starch supplement (TJ3), and the highest protein content was obtained from the medium without corn starch supplement (TJ0=24.69%). The increase of mycelial and fruitbody weight from the medium with the addition of corn material indicated a prospective in cultivation process, however effort to increase protein content of the fruit body needs further research. Cultivating P. ostreatus in mycelial phase may take shorter incubation time, may be produced in mass production with less space consuming, and higher protein content than that by producing fruitbody.
Effectiveness of Pleurotus ostreatus Extract Through Cytotoxic Test and Apoptosis Mechanism of Cervical Cancer Cells Ekowati, Nuraeni; Mumpuni, Aris; Muljowati, Juni Safitri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.7546

Abstract

Pleurotus ostreatus is a common mushroom cultivated in Indonesia, and potential properties of bioactive compounds for medicinal mushroom. This study was aimed at obtaining P.ostreatus extract bioactive compounds potential in inhibiting the proliferation of cervical cancer cells (HeLa) and evaluating the HeLa cell proliferation kinetics and HeLa cell death mechanisms. The research was beneficial in making this product can be easily applied in a more controlled industrial scale. Anticancer activity test through a cytotoxic test using the MTT [3- (4,5-dimetiltiazol-2-yl) -2.5-diphenyl tertrazolium bromide], the kinetics proliferation of HeLa cells and HeLa cell death mechanism was performed. Linear regression analysis was used to analyze the data. Ethyl acetate extract of P. ostreatus isolated from Madiun showed the best results with IC 50 = 107.59 g / ml. HeLa cell proliferation kinetics analysis showed that the application of bioactive compounds 100 g / ml resulted in an increase of in death of HeLa cells along with length of incubation time. An important finding was that HeLa cells death by apoptosis was greater than by necrosis. In conclusion, the extracts of P. ostreatus has the potential to inhibit the growth of HeLa cells.
PENGARUH PEMAPARAN SINAR GAMMA ISOTOP COBALT-60 DOSIS 0,25–1 kGy TERHADAP DAYA ANTAGONISTIK Trichoderma harzianum PADA Fusarium oxysporum Priyo Wahyudi; Untung Suwahyono; Harsoyo; Aris Mumpuni; Dwi Wahyuningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 2 (2005): June 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/449

Abstract

Trichoderma harzianum is a well-known mycoparasitic fungus that has been used as biocontrol agent of many phytopathogenic fungi. One of the effort to improve the ability of wild strain of T. harzianum in its antagonistic activity is by exposed them in gamma ray iradiation. In this experiment wild strain of T. harzianum iradiated gamma ray of Cobalt-60 (0.25 kGy, 0.5 kGy, 0.75 kGy, and 1 kGy), then assess the effect of the iradiation on its growth, the antagonistic activity and chitinase activity toward Fusarium oxysporum. Results showed that iradiation of gamma ray 0.25–1 kGy has no effect on the growth of T. harzianum and its antagonistic activity, but it significantly influence the chitinase activity. Probably the fungi have repaired the damage of DNA caused by iradiation, so that the growth and even the enzymatic function has no longer affected.
Textile Wastewater Decolorization by Pleurotus ostreatus in Organic Material Board Media Citra Mawada Entristiana; Ratna Stia Dewi; Aris Mumpuni
Journal Omni-Akuatika Vol 18 (2022): Omni-Akuatika Special Issue 4th Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2022.18.S1.981

Abstract

The textile industry in one of the fastest industries that grow today. However, the increased activity makes the production of liquid wastewater also increased because the use of water reaches 80% in production. The wastewater can endanger the aquatic ecosystems because it contains toxic dyes. Pleurotus ostreatus has ligninolytic enzymes that are capable of degrading synthetic dyes into non-toxic forms to the environment. This study aims to determine the optimum contact time of P. ostreatus in organic material board media for the best result in textile wastewater decolorization. This research used an experimental method. The research consisted preparation of P. ostreatus isolate, making of seed media, inoculation into seed media, making of log media, inoculation into log media, making of board media, and decolorization process. The parameters tested were different contact time (24, 48, 72, 96, 120, 144, and 168 hours), Total Dissolved Solid (TDS), pH, and temperature. The result of this research was that the treatment of P. ostreatus in organic material board media can decolorize textile wastewater. The value of highest decolorization percentage was 35,69% at 24 hours contact time. There are change of TDS, pH, and temperature on before and after namely 835 to 566, 8.7 to 7.62, 28.3 to 31. This board system is promising for wastewater treatment.Keywords:decolorization, textile wastewater, P. ostreatus, board media
BIO-PULPING BAGASE SEBAGAI BAHAN PEMBUATAN KERTAS DENGAN MENGGUNAKAN SPESIES JAMUR PELAPUK PUTIH DAN WAKTU INKUBASI Vio Indah Budiarti; Nuniek Ina Ratnaningtyas; Aris Mumpuni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.1798

Abstract

The Biopulping is defined as the biological process of lignin degradation by utilizing microorganisms that produce some enzymes. A microorganism which widely known in the degradation of lignin and cellulose is a group of white-rot fungi. The aims for this research to know the most effective white rot fungi species of G.lucidum, P.tuber-regium, and T.versicolor in the degradation of lignin and cellulose with different incubation time on bagasse substrate. The effectivity of biopulping indicated by the highest degradation of lignin concentration and the lowest degradation of cellulose concentration. This study used an experimental design with Completely Randomized design with a two factorial pattern. The independent variable of this study is white rot fungi species and incubation time while the dependent variable is the concentration of lignin and cellulose. The main parameter was the concentration of lignin and cellulose, supporting parameters were pH, weight loss of substrate and mycelial growth. The result showed the degradation of lignin and cellulose in all treatment. The best degradation of lignin and cellulose showed by species T.versicolor and P.tuber-regium within 30 days of incubation
Pengaruh Cercospora sp. terhadap Kandungan Asam Askorbat pada MekanismePatogenisitas Bercak Daun Tanaman Cabai : Kajian secara In vitro dan In planta Nasriyatun Yuliawati; Aris Mumpuni; Juni Safitri Muljowati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.143 KB) | DOI: 10.20884/1.bioe.2020.2.2.1896

Abstract

Red chili is a vegetable commodity that has high economic value in Indonesia. Leaf spot disease caused by the fungus Cercospora sp. is one of the limiting factors in red chili production. The occurrence of leaf spot disease is determined by the success of the pathogenesis by the fungus Cercospora sp. In addition, red chilies that are resistant to leaf spot disease have higher ascorbic acid content than vulnerable red chilies. The purpose of this study was to determine the ability to grow pathogens Cercospora sp. on the medium which was given ascorbic acid and know the effect of inoculation of the pathogen Cercospora sp. against ascorbic acid content in red chili leaves (C. annuum L.). This study used an experimental design with a completely randomized design (CRD). In vitro tests carried out consisted of PDA and PDB medium which were given ascorbic acid with a concentration of 0 mg.l-1, 0,25 mg.l-1, 0,5 mg.l-1, 0,75 mg.l-1 and 1,0 mg.l-1. In planta testing was using hot chili red chili varieties, large red chili varieties and curly red chili varieties. The treatments that were tested included calculation of disease intensity and ascorbic acid content in red chili leaves. In vitro test the main parameters observed were the diameter colony of the fungus Cercospora sp. dan mycelium dry weight. In planta test the main parameters observed were the intensity of the disease, while the supporting parameters were the incubation period of the disease, the content of ascorbic acid in the red chili leaves, temperature and humidity. In vitro test data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level, then the treatment that gave a real or very real difference was followed by the Least Significant Difference test (LSD). In planta test data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level, then the treatment that gave a real or very real difference was followed by the Least Significant Difference test (LSD). The results showed that the pathogen Cercospora sp. able to grow well on the PDA medium and GDP medium which were given ascorbic acid. Inoculation of pathogen Cercospora sp. can increase ascorbic acid content in red chili leaves.
Persentase Penyakit yang disebabkan oleh Jamur pada Buah Cabai Merah (Capsicum annuum L.) di Desa Serang Kecamatan Karangreja Kabupaten Purbalingga Bella Ajeng Ayu Kirana; Eddy Tri Sucianto; Aris Mumpuni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 4 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3389

Abstract

Cabai merah (Capsicum annuum L.) merupakan salah satu tanaman hortikultura yang penting dalam kehidupan sehari-hari. Kebutuhan cabai merah kian hari terus meningkat karena banyaknya yang memanfaatkan sebagai bahan dasar untuk pembuatan berbagai jenis makanan. Cabai merah juga banyak dimanfaatkan sebagai bahan baku industri pangan dan farmasi. Penduduk di Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga umumnya berprofesi sebagai petani. Para petani mengalami beberapa kendala dalam meningkatkan produksi tanaman cabai merah (C. annuum L.) karena adanya organisme pengganggu tanaman seperti hama dan patogen. Penelitian ini bertujuan untuk mengetahui jenis penyakit yang disebabkan oleh jamur pada buah cabai merah (C. annuum L.), mengetahui jamur yang menyebabkan penyakit pada buah cabai merah (C. annuum L.) serta mengetahui besarnya nilai persentase penyakit yang disebabkan oleh jamur pada buah cabai merah (C. annuum L.) di Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga. Penelitian ini dilakukan dengan menggunakan metode survai dengan pengambilan sampel secara random sampling pada 2 lokasi berbeda di Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga. Identifikasi penyakit dilakukan dengan melihat tanda dan gejala. Identifikasi jamur dilakukan dengan melihat karakterisitik secara mikroskopis dan makroskopis, kemudian dihitung persentase penyakit pada buah cabai merah (C. annuum L.), selanjutnya dilakukan uji Postulat Koch. Hasil penelitian ini diperoleh satu jenis penyakit yang terdapat pada buah cabai merah (C. annuum L.) di Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga yaitu penyakit antraknosa yang disebabkan oleh jamur Colletotrichum sp. Persentase penyakit antraknosa yang disebabkan oleh jamur Colletotrichum sp sebesar 51,1%.
PENGARUH Colletotrichum coccodes TERHADAP KANDUNGAN ASAM ASKORBAT PADA MEKANISME PATOGENISITAS ANTRAKNOSA TANAMAN CABAI: KAJIAN SECARA IN VITRO DAN IN PLANTA fadhila meilasari; Juni Safitri Muljowati; Aris Mumpuni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.766 KB) | DOI: 10.20884/1.bioe.2020.2.2.1918

Abstract

Patogen Colletotrichum coccodes merupakan salah satu patogen yang dapat menginfeksi tanaman cabai dan menyebabkan penyakit antraknosa terutama pada bagian buah dan daun. Tanaman dengan kandungan asam askorbat tinggi memiliki ketahanan yang lebih tinggi terhadap serangan patogen. Tanaman cabai yang tahan memiliki kandungan asam askorbat yang lebih tinggi dibandingkan dengan tanaman cabai toleran maupun rentan. Tujuan penelitian ini adalah mengetahui kemampuan tumbuh patogen C. coccodes pada medium yang diberi asam askorbat dan mengetahui pengaruh inokulasi patogen C. coccodes terhadap kandungan asam askorbat pada daun cabai. Penelitian dilakukan di Laboratorium Mikologi dan Fitopatologi, Laboratorium Lingkungan, & Greenhouse Fakultas Biologi Universitas Jenderal Soedirman, Purwokerto. Penelitian ini menggunakan dua uji yaitu uji in vitro dan uji in planta dengan metode eksperimental Rancangan Acak Lengkap (RAL), Uji in vitro menggunakan A) Medium PDA diberi asam askorbat; B) Medium PDB diberi asam askorbat dengan perlakuan penambahan asam askorbat sebanyak 0 mg.L-1 (kontrol); 0,25 mg.L-1; 0,50 mg.L-1; 0,75 mg.L-1; dan 1 mg.L-1, diulang sebanyak lima kali. Variabel bebas yang digunakan yaitu berbagai dosis asam askorbat, variabel terikatnya adalah pertumbuhan patogen C. coccodes. Parameter utama yaitu diameter koloni dan bobot kering miselium. Uji in planta menggunakan tiga varietas cabai (V1: Cabai merah hot chili; V2 Cabai merah keriting; V3: Cabai merah besar), uji A) Uji intensitas penyakit pada tanaman dan B) Uji kandungan asam askorbat. Masing-masing kelompok uji diulang sebanyak lima kali. Variabel bebas yang digunakan adalah varietas cabai merah, variabel terikatnya adalah nilai kerusakan tanaman berdasarkan kategori yang diamati pada waktu pengamatan yang ditentukan. Parameter utama yaitu intensitas penyakit, dan parameter pendukung yaitu periode masa inkubasi, kandungan asam askorbat pada daun cabai, temperatur, kelembaban dan pH tanah. Data uji in vitro dan uji in planta yang diperoleh dianalisis menggunakan analisis ragam (ANOVA) dengan tingkat kepercayaan 95%, dan perlakuan yang memberikan perbedaan nyata dilanjutkan dengan Uji Beda Nyata Jujur (BNJ) pada tingkat kepercayaan 95%. Hasil penelitian pada uji in vitro menujukkan bahwa patogen C. coccodes memiliki kemampuan tumbuh yang baik pada medium PDA dan medium PDB dengan penambahan asam askorbat. Hasil penelitian pada uji in planta, inokulasi patogen C. coccodes pada daun cabai merah dapat meningkatkan kandungan asam askorbat pada tanaman cabai merah Kata kunci : Colletotrichum coccodes, Cabai Merah, Antraknosa, Asam askorbat.
Pengaruh Pemberian Inokulum Fungi Mikoriza Arbuskula (FMA) Campuran terhadap Kemunculan Penyakit Layu Fusarium pada Tanaman Mentimun (Cucumis sativus L.) dan Melon (Cucumis melo L.) Nadya Sofia Siti Sa'adah; Uki Dwiputranto; Aris Mumpuni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 4 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2661

Abstract

Cucumber (Cucumis sativus L.) and Melon (Cucumis melo L.) are examples of vegetables and fruit that are widely consumed by the community, and also have many benefits. The market demand for cucumbers and melons is very high. Therefore the production should meet the demand. There are several problems in production, and one of them is wilt caused by Fusarium. Conventionally, the use of synthetic fungicides is considering as the right solution for controlling fusarium wilt. However, taking into account the harmful effects of these fungicides, the use of Arbuscular Mycorrhizal Fungi (AMF) is a choice made. This study used a Completely Randomized Design (CRD) of mixed AMF inoculums (0, 5, 10, 15, 20 g FMA with zeolite/plant carrier medium). The main parameters observed were disease intensity, while the supporting parameters observed were pH, temperature, air humidity, disease incubation period, and degree of infection. The data obtained were analyzed using a Variety Test (F test) with a Standard Error of 5%. Based on the research result, plants which are inoculated by mycorrhizae inoculum is more resistant to fusarium wilt disease. The optimal dose of AMF mixture to reduce the intensity of fusarium wilt in cucumber (Cucumis sativus L.) plants is M3BT (AMF inoculation of 15 g / plant mixture), and melon (Cucumis melo L.) is M2BM (10 g / plant AMF mixture inoculation)
Co-Authors Agustina Nursanti Amanda, Safira angelin marhavyna cristy Arif Rahman Hikam Bella Ajeng Ayu Kirana Budiarti, Vio Indah Citra Mawada Entristiana cristy, angelin marhavyna Dwi Wahyuningsih Dwiana Muflihah Yulianti Eddy Tri Sucianto Endang Sri Purwati fadhila meilasari Haqq, Insaaniy Mahdiyatul Harsoyo hasanah, Amanda rohmatun Hikam , Arif Rahman Imam Widhiono Insaaniy Mahdiyatul Haqq Juni Safitri Muljowati Kirana, Bella Ajeng Ayu meilasari, fadhila Mubarrak, Syifa Nadya Sofia Siti Sa'adah Nasriyatun Yuliawati Nugraha, Tiara Hadi Nuniek Ina Ratnaningtyas Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Nursanti, Agustina Priyo Wahyudi Purnomowati Purnomowati Purnomowati, Purnomowati Rahma, Salma Aulia Rahmadianti, Maghfira Rizki Ratna Stia Dewi Ratna Stia Dewi Ratna Stia Dewi Ratnaningtyas, ‪Nuniek Ina Safira Amanda Saputra, Wahyu Dwi Setio, Adinda Eka Murti Siti Sa'adah, Nadya Sofia Slamet Risyanto Sucianto, Eddy Tri Syifa Mubarrak Uki Dwiputranto Uki Dwiputranto Untung Suwahyono Vio Indah Budiarti Wahyu Dwi Saputra Wardaya, Yasenia Sandra Yulianti, Dwiana Muflihah Yuliawati, Nasriyatun