Garuda - Garba Rujukan Digital

PENGARUH PEMBERIAN EKSTRAK DAUN KELOR (MORINGA OLEIFERA L.) DOSIS BERTINGKAT TERHADAP GAMBARAN MIKROSKOPIS GASTER TIKUS WISTAR JANTAN YANG DIINDUKSII FORMALIN Taufik Setiawan; Neni Susilaningsih; Fanti Saktini
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 7, No 2 (2018): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Latar Belakang: Kelor memiliki zat yang bermanfaat sebagai gastroproteksi, antiulkus, dan antioksidan. Salah satu penyebab kerusakan gaster adalah akibat paparan formalin yang merupakan zat iritatif dan karsinogenik yang sering disalahgunakan sebagai pengawet makanan. Kelor berpotensi mencegah kerusakan gaster yang disebabkan oleh paparan formalin.Tujuan: Mengetahui pengaruh pemberian ekstrak daun kelor (Moringa oleifera) dosis bertingkat pada gambaran histopatologis mukosa gaster tikus wistar yang dinduksi formalin.Metode: Jenis penelitian ini adalah true eksperimental laboratorik dengan Post Test Only with Control Group Design. Sampel sebanyak 25 ekor tikus wistar jantan yang memenuhi kriteria inklusi dan eksklusi, diadaptasi selama 7 hari, diberi pakan dan minum standar. Kelompok kontrol negatif tidak diberi perlakuan apapun, kontrol positif diberikan aquadest selama 5 hari dan dilanjutkan formalin peroral 100 mg/kgBB/hari selama 21 hari. Kelompok P1, P2, dan P3 diberi ekstrak daun kelor pada 5 hari pertama, dengan dosis 200, 400, dan 800 mg/kgBB/hari. Selanjutnya diberi formalin 100 mg/kgBB/hari dan ekstrak daun kelor sesuai dengan dosis awal selama 21 hari. Setelah 26 hari,tikus wistar diterminasi, diambil organ gaster, dan dilakukan pemeriksaan histopatologi mukosa gaster berupa ulserasi, erosi, dan deskuamasi menggunakan skor Barthel Manja.Hasil: Rerata kerusakan mukosa gaster tertinggi terdapat pada kelompok kontrol positif. Uji Mann-whitney menunjukkan perbedaan yang bermakna (p<0,05) antara rerata kelompok Kontrol (+) dengan rerata kelompok P1, P2, P3 dan Kontrol (-).Simpulan: Pemberian ekstrak daun kelor (Moringa oleifera) dosis bertingkat berpengaruh terhadap gambaran histopatologis mukosa gaster tikus yang wistar diinduksi formalin. Semakin tinggi dosis ekstrak daun kelor maka semakin rendah derajat kerusakan pada gambaran mikroskopis gaster tikus wistar jantan yang diinduksi formalin.

PENGARUH PEMBERIAN EKSTRAK DAUN SIRIH MERAH (Piper Crocatum) DOSIS BERTINGKAT TERHADAP AKTIVITAS FAGOSITOSIS MAKROFAG MENCIT BALB/C YANG DIINFEKSI Salmonella typhimurium Citra Hutami Saraswati; Ratna Damma Purnawati; Neni Susilaningsih
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Latar Belakang : Sirih merah (Piper crocatum) merupakan salah satu tanaman obat yang multi khasiat. Daun Piper crocatum memiliki kandungan diantaranya tanin, saponin, alkaloid dan flavonoid. Senyawa alkaloid dan flavonoid meningkatkan aktivitas IL-2 dan proliferasi limfosit. Sel Th1 yang teraktivasi akan mempengaruhi SMAF (Spesific Makrofag Activating Factor) yang dapat mengaktifkan makrofag. Penelitian ini bertujuan untuk mengevaluasi pengaruh ekstrak sirih merah terhadap daya tahan mencit yang terinfeksi Salmonella typhimurium dengan menilai kemampuan fagositosis makrofag.Tujuan : Untuk mengetahui pengaruh pemberian ekstrak daun sirih merah dosis 10, 30, 100 mg/hari/mencit terhadap aktivitas fagositosis makrofag mencit Balb/c yang diinfeksi Salmonella typhimurium.Metode : Penelitian ini merupakan penelitian eksperimental laboratorik dengan desain Post Test Only Control Group Design. Penelitian ini menggunakan 5 kelompok, yaitu kelompok kontrol yang terdiri dari K1 yang hanya diberikan ekstrak daun Piper crocatum 10 mg/hari/mencit dan K2 yang hanya diberikan injeksi intraperitoneal Salmonella typhimurium serta kelompok perlakuan (P1,P2,P3) yang diberikan injeksi intraperitoneal Salmonella typhimurium dan ekstrak daun Piper crocatum dosis berturut-turut 10,30,100 mg/hari/mencit.Hasil : Rerata indeks fagositosis makrofag masing-masing kelompok : K1 = 0,22; K2 = 0,14; P1 = 0,23; P2 = 0,32; P3 = 0,66. Indeks fagositosis makrofag antara kelompok kontrol dengan perlakuan dan antar kelompok perlakuan terdapat perbedaan yang signifikan, yaitu antara K1-K2, K2-P1, K2-P2, K2-P3, P1-P2, P1-P3, dan P2-P3.Simpulan : Pemberian ekstrak daun sirih merah dosis bertingkat berpengaruh terhadap peningkatan aktivitas fagositosis makrofag.

Therapeutic Potential of Curcumin in Modulating the HMGB1/TLR4/NF-κB Axis in Polymicrobial Peritonitis: A Systematic Review and Dose-Response Meta-Analysis Leonardo Aaron Hartanto; Neni Susilaningsih; Erik Prabowo
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 2 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i2.1516

Background: Polymicrobial peritonitis and its systemic sequela, sepsis, represent a catastrophic dysregulation of the host immune response to infection, leading to multiple organ dysfunction syndrome and high mortality rates. The pathophysiology is driven by a hyperinflammatory cytokine storm followed by immunoparalysis, governed centrally by the high mobility group box 1 (HMGB1)/toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) signaling axis. Curcumin, a polyphenolic compound derived from Curcuma longa, has demonstrated potent immunomodulatory properties. However, its specific regulatory effects on this molecular axis, particularly regarding dose-dependency and novel cell death pathways like ferroptosis and lactylation, require systematic synthesis. Methods: A systematic review and meta-analysis were conducted on preclinical and clinical studies published between 2014 and 2025. Ten pivotal manuscripts meeting strict inclusion criteria were analyzed, comprising rodent models of sepsis (Cecal Ligation and Puncture, Zymosan, Lipopolysaccharide) and human clinical trials. Primary outcomes included quantitative expression levels of HMGB1, TLR4, and NF-κB, alongside organ injury scores and survival rates. Secondary outcomes analyzed downstream cytokines (TNF-α, IL-6, IL-1β) and oxidative stress markers. Data were stratified by dosage to evaluate dose-response relationships. Results: The analysis included data from 218 subjects. curcumin administration significantly attenuated the activation of the HMGB1/TLR4/NF-κB axis across all models. Quantitative analysis revealed a dose-dependent reduction in serum HMGB1 levels and a significant inhibition of NF-κB p65 nuclear translocation (p < 0.001). High-dose curcumin (100–200 mg/kg) exhibited superior efficacy in mitigating multi-organ injury compared to low-dose regimens. Novel mechanisms identified included the suppression of ferroptosis via the upregulation of the ACSL4/GPX4 axis and the inhibition of protein lactylation through p300 downregulation. Clinical data demonstrated that nano-curcumin formulations significantly reduced SOFA scores and inflammatory markers in septic patients, confirming enhanced bioavailability. Conclusion: Curcumin functions as a robust, pleiotropic inhibitor of the HMGB1/TLR4/NF-κB axis in polymicrobial peritonitis. Its therapeutic efficacy is dose-dependent and involves the regulation of emerging epigenetic and cell death pathways. These findings support the clinical integration of nano-curcumin as an adjuvant therapy for surgical sepsis.

Therapeutic Potential of Curcumin in Modulating the HMGB1/TLR4/NF-κB Axis in Polymicrobial Peritonitis: A Systematic Review and Dose-Response Meta-Analysis Leonardo Aaron Hartanto; Neni Susilaningsih; Erik Prabowo
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 2 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i2.1516

Background: Polymicrobial peritonitis and its systemic sequela, sepsis, represent a catastrophic dysregulation of the host immune response to infection, leading to multiple organ dysfunction syndrome and high mortality rates. The pathophysiology is driven by a hyperinflammatory cytokine storm followed by immunoparalysis, governed centrally by the high mobility group box 1 (HMGB1)/toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) signaling axis. Curcumin, a polyphenolic compound derived from Curcuma longa, has demonstrated potent immunomodulatory properties. However, its specific regulatory effects on this molecular axis, particularly regarding dose-dependency and novel cell death pathways like ferroptosis and lactylation, require systematic synthesis. Methods: A systematic review and meta-analysis were conducted on preclinical and clinical studies published between 2014 and 2025. Ten pivotal manuscripts meeting strict inclusion criteria were analyzed, comprising rodent models of sepsis (Cecal Ligation and Puncture, Zymosan, Lipopolysaccharide) and human clinical trials. Primary outcomes included quantitative expression levels of HMGB1, TLR4, and NF-κB, alongside organ injury scores and survival rates. Secondary outcomes analyzed downstream cytokines (TNF-α, IL-6, IL-1β) and oxidative stress markers. Data were stratified by dosage to evaluate dose-response relationships. Results: The analysis included data from 218 subjects. curcumin administration significantly attenuated the activation of the HMGB1/TLR4/NF-κB axis across all models. Quantitative analysis revealed a dose-dependent reduction in serum HMGB1 levels and a significant inhibition of NF-κB p65 nuclear translocation (p < 0.001). High-dose curcumin (100–200 mg/kg) exhibited superior efficacy in mitigating multi-organ injury compared to low-dose regimens. Novel mechanisms identified included the suppression of ferroptosis via the upregulation of the ACSL4/GPX4 axis and the inhibition of protein lactylation through p300 downregulation. Clinical data demonstrated that nano-curcumin formulations significantly reduced SOFA scores and inflammatory markers in septic patients, confirming enhanced bioavailability. Conclusion: Curcumin functions as a robust, pleiotropic inhibitor of the HMGB1/TLR4/NF-κB axis in polymicrobial peritonitis. Its therapeutic efficacy is dose-dependent and involves the regulation of emerging epigenetic and cell death pathways. These findings support the clinical integration of nano-curcumin as an adjuvant therapy for surgical sepsis.

Title

Found 14 Documents
Search

Abstract

Abstract

Abstract

Abstract

Title Search

Found 14 Documents Search

Abstract

Abstract

Abstract

Abstract

Title

Found 14 Documents
Search