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All Journal Jurnal Keperawatan Soedirman (The Soedirman Journal of Nursing) Jurnal Kebidanan Dan Kesehatan Tradisional IJNP (Indonesian Journal of Nursing Practices) Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Indonesian Journal of Chemistry JURNAL PENDIDIKAN TAMBUSAI Jurnal Perawat Indonesia Journal Of Bionursing Jurnal Aisyah : Jurnal Ilmu Kesehatan Science Midwifery Caring : Indonesian Journal of Nursing Science Indonesian Nursing Journal of Education and Clinic (Jurnal Pendidikan dan Praktik Keperawatan Indonesia) Jurnal of Community Health Development (JCHD) Holistik Jurnal Kesehatan
Mekar Dwi Anggraeni
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Development of a Low-Cost Spectrophotometer for Protein Determination Using the Lowry Method Salsabilla, Lestha Aurel; Hermawan, Dadan; Diastuti, Hartiwi; Anggraeni, Mekar Dwi; Fatoni, Amin
Indonesian Journal of Chemistry Vol 26, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.99361

Abstract

Affordable and accessible laboratory equipment is increasingly crucial, particularly in resource-limited settings. This research presents the development of a cost-effective spectrophotometer using an Arduino microcontroller and a TSL2591 light sensor to quantify protein concentration via the Lowry method. The device employs a 750 nm LED and a 3D-printed PLA case to house the components. Casein was used for calibration, yielding a linear detection range of 100–700 ppm with a high correlation coefficient (r = 0.9991). The limit of detection (LOD) and limit of quantification (LOQ) were 18.05 and 60.16 ppm, respectively. Precision was confirmed with a HORRAT value of 0.982. Accuracy was validated using the Wilcoxon Signed-Rank test, which showed no significant difference between the fabricated device and a commercial UV-vis spectrophotometer (p = 0.779). These findings support the reliability and reproducibility of the device for protein quantification. The project’s open-source nature encourages further development and application in educational, clinical, and field settings. This innovation supports the development of accessible, low-cost biochemical analysis tools.
Pengaruh program pelatihan keperawatan terhadap penurunan kejadian ventilator associated pneumonia (VAP), peningkatan pengetahuan, praktik, dan kepatuhan perawat: Sebuah tinjauan sistematis Rukhayati, Yati; Anggraeni, Mekar Dwi
Holistik Jurnal Kesehatan Vol. 19 No. 10 (2025): Volume 19 Nomor 10
Publisher : Program Studi Ilmu Keperawatan-fakultas Ilmu Kesehatan Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/hjk.v19i10.1623

Abstract

Background: Patients admitted to the Intensive Care Unit (ICU) and on ventilators for more than 48 hours are highly susceptible to Ventilator-Associated Pneumonia (VAP), a common clinical complication. A study showed that training critical care nurses in implementing a VAP prevention package effectively reduced the incidence of VAP. Purpose: To identify the effect of a critical care training program on the incidence of VAP and improve nurses' knowledge, practice, and adherence. Method: The literature review included a comprehensive search of academic databases for relevant studies, including PubMed, Scopus, Wiley Online Library, MJSonline, and Google Scholar. A quasi-experimental study of intensive care nurses providing care to patients on mechanical ventilators published between 2015 and 2024 was conducted. Results: Ten reviewed research articles demonstrated that a nurse training program can reduce the incidence of VAP and improve nurses' knowledge, practice, and adherence to VAP prevention protocols. Conclusion: The nurse training program reduced the incidence of VAP and improved nurses' knowledge, practice, and adherence in the intensive care unit. Suggestion: Future research should expand access to the literature, implement purposive sampling, and optimize resources to generate deeper insights and improve VAP prevention strategies in the ICU.   Keywords: Adherence; Knowledge; Nursing Training Program; Practice; Ventilator-Associated Pneumonia (VAP).   Pendahuluan: Pasien yang dirawat di Intensive Care Unit (ICU) dan menggunakan ventilator selama lebih dari 48 jam sangat rentan mengalami Ventilator-Associated Pneumonia (VAP), sebuah komplikasi klinis yang cukup umum. Sebuah studi menunjukkan bahwa, pelatihan perawat perawatan kritis dalam penerapan paket pencegahan VAP secara efektif mengurangi insiden VAP . Tujuan: Untuk mengidentifikasi pengaruh program pelatihan keperawatan terhadap kejadian VAP, peningkatan pengetahuan, praktik, dan kepatuhan perawat. Metode: Tinjauan pustaka yang melibatkan penelusuran basis data akademik daring untuk mendapatkan studi-studi relevan, termasuk PubMed, Scopus, Wiley Online Library, MJSonline, dan Google Scholar. Studi kuasi-eksperimental tentang perawat perawatan intensif yang memberikan layanan kepada pasien menggunakan ventilator mekanik yang diterbitkan antara tahun 2015 dan 2024. Hasil: Sebanyak 10 artikel penelitian yang ditinjau menunjukkan bahwa, program pelatihan perawat dapat mengurangi kejadian VAP dan meningkatkan pengetahuan, praktik, dan kepatuhan perawat terhadap protokol pencegahan VAP. Simpulan: Program pelatihan perawat mengurangi kejadian VAP dan meningkatkan pengetahuan, praktik serta kepatuhan perawat di unit perawatan intensif. Saran: Penelitian selanjutnya harus memperluas akses literatur, menerapkan seleksi objektif, dan mengoptimalkan sumber daya untuk menghasilkan wawasan yang lebih dalam dan meningkatkan strategi pencegahan VAP di ICU.   Kata Kunci: Kepatuhan; Pengetahuan; Praktik; Program Pelatihan Keperawatan; Ventilator Associated Pneumonia (VAP).
Co-Authors Agus Prastowo AMIN FATONI Arif Setyo Upoyo Dadan Hermawan, Dadan Eni Rahmawati Erni Setiyowati Hartati, Hartiwi Diastuti Haryanti, Hernita Kus Ima Rismawati Iwan Purnawan Kartikasari, Aprilia Kristantia, Ziella Ruth Linda Nurul Hadiati Lutfatul Latifah Mursiyam, Qathrin Nada Rahayu Astuti Rukhayati, Yati Ryan Hara Permana Salsabilla, Lestha Aurel Saryono Saryono Saryono, Setiani, Eka Titi setiawati, nina Setiyani, Rahmi Sidik Awaludin Tuswati, Tuswati Ulfatul Anisah Wahyu Ekowati