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All Journal HAYATI Journal of Biosciences Jurnal Biologi Udayana AL KAUNIYAH Jurnal Matematika & Sains Journal of Mathematical and Fundamental Sciences Jurnal Sumberdaya Hayati The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS) Indonesian Journal of Chemistry Medika Kartika : Jurnal Kedokteran dan Kesehatan 3BIO: Journal of Biological Science, Technology and Management Berkala Penelitian Hayati
Anggraini Barlian
Biology Study Program, School Of Life Sciences And Technology Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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Journal : 3BIO: Journal of Biological Science, Technology and Management

 1 
The Protective Effect of Propolis Nanoemulsion (NEP) Against UVB Irradiation Induced Photoaging in Human Dermal Fibroblast (HDF) Anggraini Barlian; Rini Agustin; Ramadhani Eka Putra
3BIO: Journal of Biological Science, Technology and Management Vol. 2 No. 2 (2020)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2020.2.2.1

Abstract

The skin damage induced by UV irradiation can cause photoaging as a consequence of reactive oxygen species (ROS) generation. Propolis is one of the most abundant natural product sources of polyphenols and derived from various plant resins collected by honeybees. This study aimed to determine the due administration of the propolis nanoemulsion (NEP) as an anti-photoaging by observing the concentration of intracellular ROS and lipid peroxides in human dermal fibroblast (HDF) by exposure to UVB. The cytotoxic of NEP was tested by the MTT assay, intracellular ROS was measured using the DCFDA assay, lipid peroxide products were analyzed by the TBARS assay and cell nuclei was observed by DAPI staining. PSA analysis revealed that the sizes of NEP were about 131.9 to 131 nm. The optimum concentration of NEP was 0,1μg / ml. ROS production in HDF treatment of NEP significantly decreases (p <0.05). Lipid peroxide products showed the same pattern as ROS concentration. The lipid peroxide concentration in the treatment NEP significantly decreases (p<0.05). In conclusion, the propolis in NEP acts as an antioxidant and has the potential to reduce the production of ROS and lipid peroxides caused by exposure to UVB.
Aging Process in Dermal Fibroblast Cell Culture of Green Turtle (Chelonia mydas) Anggraini Barlian; Yemima D Riani
3BIO: Journal of Biological Science, Technology and Management Vol. 2 No. 2 (2020)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2020.2.2.2

Abstract

Senescence in green turtle is an interesting process to study because until now no clear explanation has been established about senescence at cellular or molecular level in this species. One of common markers used for detecting senescence is telomere shortening. Reduced telomerase activity may also cause senescence. The aims of this research are to establish and identify dermal fibroblast cell culture from green turtle and also to compare telomere length and telomerase activity from the cells subculture 5 and 14. Cells were identified with Rabbit Anti-Vimentin Polyclonal Antibody and Goat Polyclonal Antibody using confocal microscope. Telomere length was obtained using TeloTAGGG Telomere Length Assay (Roche), while telomerase activity was obtained using TeloTAGGG Telomerase PCR ElisaPlus. Primary cell culture from green turtle skin showed fibroblastic morphology and immunocytochemistry results using vimentin antibody proved that the culture was fibroblast cell. Measurement of telomere length and telomerase activity showed that telomere length and telomerase activity of subculture 14 was greater than subculture 5. However, based on morphology, green turtle fibroblast skin cell culture showed senescent morphology. Therefore, possible aging mechanism that the green turtle fibroblast skin cell culture underwent did not go through both telomere shortening and reduced telomerase activity.
Co-Authors Ahmad Ridwan Akfiny Hasdi Aimon Arie Wibowo Atik Choirul Hidajah C Hanny Wijaya Christofora Hanny Widjaja Diah Ratnadewi Dian Anggraeny Ekavianty Prajatelistia Ergha Widya Sarjana Fathan Aditya Sanjaya Fitria D. Ayuningtyas Fitriyatul Qulub Hari Basuki Notobroto Helmi Majid Ar Rasyid Hermawan Judawisastra Hermawan Judawisastra Hidayah, Thauhidayatul Hidayah, THAUHIDAYATUL HIDAYAH Hutomo Tanoto Iis Rosliana Iis Rosliana Iis Rosliana Ika Dewi Ana Ika Dewi Ana Imam Rosadi Indra Wibowo Indra Wibowo Irsyah Afini Irsyah Afini Irsyah Afini Karina Karina Komang A. Wahyuningsih Komang A. Wahyuningsih Kusnandar Anggadiredja Marselina Tan Muhammad Bagas Ananda Ni Luh Wisma Eka Yanti Noviana Vanawati Noviana Vanawati Prihartini Widiyanti Raden Tanita Astrian Ramadhani Eka Putra Regina Giovanni Rini Agustin Rizka Musdalifah Amsar Rizka Musdalifah Amsar Safira Meidina Nursatya Salindri Prawitasari Siti Sobariah Siti Sobariah Siti Sobariah Soraya Rahmanisa Tami Bachrurozy Tias Widyastuti Tias Widyastuti Tias Widyastuti Triati Dewi Kencana Wungu Untung Ari Wibowo Yemima D Riani Yemima Dani Riani Zuliyanto Zakaria