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All Journal HAYATI Journal of Biosciences Jurnal Biologi Udayana AL KAUNIYAH Jurnal Matematika & Sains Journal of Mathematical and Fundamental Sciences Jurnal Sumberdaya Hayati The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS) Indonesian Journal of Chemistry Medika Kartika : Jurnal Kedokteran dan Kesehatan 3BIO: Journal of Biological Science, Technology and Management Berkala Penelitian Hayati
Anggraini Barlian
Biology Study Program, School Of Life Sciences And Technology Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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Journal : HAYATI Journal of Biosciences

 1 
Isolation of Native Plant-Derived Exosome-like Nanoparticles and Their Uptake by Human Cells Diah Ratnadewi; Christofora Hanny Widjaja; Anggraini Barlian; Rizka Musdalifah Amsar; Ika Dewi Ana; Atik Choirul Hidajah; Hari Basuki Notobroto; Triati Dewi Kencana Wungu
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.182-192

Abstract

The exosome is a nano-sized vesicular particle commonly secreted from eukaryotic cells to extracellular space. Exosome functions in cellular communication, nutrients or bioactive compounds delivery, and cellular immunity. It is believed that plant-derived exosome-like particle (PDEN) potential to deliver nano-bioactive compounds to the human body. As Indonesia is one of the herbal centers of the world, we are encouraged to develop natural richness for human welfare. The study aimed to verify various methods to isolate exosomes from the fresh ginger rhizome and quina cells and examine the ginger PDENs' capacity to internalize in vitro human cells. The PDENs isolation was carried out by centrifugation and precipitation. The particles were observed through a transmission electron microscope, dynamic light scattering, and particle size analysis. Differential ultracentrifugation combined with PEG6000 provided a better size range of particles. Nanoparticles tracking analysis performed the concentration of particles within a specific size range. Ginger and quina PDENs size ranged from 40-650 nm, and the concentration was 3.6 × 107 and 2.8 × 106, respectively. The PKH67-labelled ginger PDENs were taken up by human dermal fibroblast and human Wharton's jelly mesenchymal stem cells after 30 minutes and 21 hours. DPPH assays revealed that ginger PDENs have antioxidant activity, indicating the presence of some nano-molecules in those particles. This study would be the baseline for developing ginger PDENs as a functional food with nano-ingredients, or as an effective vehicle of nano-drug, for human health.
The Properties of Exosomes Derived from Mesenchymal Stem Cells Preconditioned with L-Ascorbic Acid and Cobalt (II) Chloride Anggraini Barlian; Rizka Musdalifah Amsar; Salindri Prawitasari; Christofora Hanny Wijaya; Ika Dewi Ana; Triati Dewi Kencana Wungu
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1100-1110

Abstract

Extracellular vesicles including exosomes, are produced by cells for intracellular communication. Preconditioning of parental cells influences exosome properties. The purpose of this study was to examine the effects of L-ascorbic acid (LAA) and cobalt (II) chloride (CoCl2) on human Wharton’s jelly mesenchymal stem cell (hWJ-MSC)-derived exosomes and their ability to promote stem cell differentiation into chondrocytes. The cells were isolated from the umbilical cord and characterized according to the criteria for mesenchymal stem cell. The cells were cultured in a serum-free medium containing LAA and CoCl2. Cell-produced exosomes were isolated and characterized. hWJ-MSCs can grow in serum-free medium containing LAA and CoCl2. Exosomes derived from hWJ-MSCs had a round morphology, particle size within the exosome range, CD 63 expression, and the capacity to be internalized by cells. The production of exosomes by hWJ-MSCs was enhanced by LAA treatment. LAA and CoCl2 promoted stem cell differentiation into chondrocytes, as indicated by the production of collagen type II and glycosaminoglycans. LAA and CoCl2 affect the properties of MSC-derived exosomes. LAA induces cells to produce exosomes in greater quantities, which have the potential to promote chondrogenic differentiation of stem cells.
Co-Authors Ahmad Ridwan Akfiny Hasdi Aimon Arie Wibowo Atik Choirul Hidajah C Hanny Wijaya Christofora Hanny Widjaja Diah Ratnadewi Dian Anggraeny Ekavianty Prajatelistia Ergha Widya Sarjana Fathan Aditya Sanjaya Fitria D. Ayuningtyas Fitriyatul Qulub Hari Basuki Notobroto Helmi Majid Ar Rasyid Hermawan Judawisastra Hermawan Judawisastra Hidayah, Thauhidayatul Hidayah, THAUHIDAYATUL HIDAYAH Hutomo Tanoto Iis Rosliana Iis Rosliana Iis Rosliana Ika Dewi Ana Ika Dewi Ana Imam Rosadi Indra Wibowo Indra Wibowo Irsyah Afini Irsyah Afini Irsyah Afini Karina Karina Komang A. Wahyuningsih Komang A. Wahyuningsih Kusnandar Anggadiredja Marselina Tan Muhammad Bagas Ananda Ni Luh Wisma Eka Yanti Noviana Vanawati Noviana Vanawati Prihartini Widiyanti Raden Tanita Astrian Ramadhani Eka Putra Regina Giovanni Rini Agustin Rizka Musdalifah Amsar Rizka Musdalifah Amsar Safira Meidina Nursatya Salindri Prawitasari Siti Sobariah Siti Sobariah Siti Sobariah Soraya Rahmanisa Tami Bachrurozy Tias Widyastuti Tias Widyastuti Tias Widyastuti Triati Dewi Kencana Wungu Untung Ari Wibowo Yemima D Riani Yemima Dani Riani Zuliyanto Zakaria