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Identifikasi Mikroba dari Lendir Estrus dan Deteksi Kebuntingan Sapi Kawin Berulang di Sleman, Yogyakarta Surya Agus Prihatno; Abdul Samik; Dea Indriani Astuti; Muhammad Agil; Usamah Afiff; Anriansyah Renggaman; Dedi Rahmat Setiadi; Yosua Kristian Adi
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70916

Abstract

Repeat breeding is condition of cows that have normal or near normal estrous cycles but fail to become pregnant after several insemination. In this study, we investigated the microbes in the cervical and vaginal mucus of beef cattle and dairy cows that experience repeat breeding and detected the pregnancy after the third or more artificial insemination. A total of 14 beef cattle and 6 dairy cows that experience repeat breeding in the cattle herd in the Yogyakarta region of Indonesia were used as samples in this study. Cervical and vaginal mucus samples were collected using plastic sheet when the cow was re-estrus. The samples were put into the 5 ml Brain Heart Infusion Broth for bacterial isolation and identification. Pregnancy examination was carried out on day 45 after artificial insemination using ultrasound method. Bacteria that could be isolated and identified from cervical mucus and vaginal mucus of beef cattle and dairy cows included Bacillus sp., Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas sp. Some cows detected positive in pregnancy examination even though the bacteria were presence in the cervical and/or vaginal mucus. There was various composition of bacteria found in the cervical mucus and vaginal mucus, of beef cattle and dairy cows with repeat breeding in livestock groups in Yogyakarta. The presence of bacterial in the cervical and vaginal mucus during estrus was not always become the causes of failed pregnancy.
Nanoemulsions of Biosurfactant and Cinnamon Essential Oil: A Promising Antimicrobial Approach for Oral Pathogens Isty Adhitya Purwasena; Anriansyah Renggaman; Dzulianur Mutsla; Nurul Wahyuni; Annisa Afiva; Dea Ajeng Pratiwi
3BIO: Journal of Biological Science, Technology and Management Vol. 8 No. 1 (2026): Vol 8 No 1 (2026)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2026.8.1.2

Abstract

Dental and oral care products often contain Sodium Lauryl Sulfate (SLS), which can cause allergic reactions and mucosal infection. Biosurfactants (BS) and cinnamon essential oil (CEO) are safer alternatives due to their antimicrobial properties. This study screens BS form five bacterial isolates, CEO, and their nanoemulsions prepared by low-energy method (NEL) and high- energy method (NEH) for inhibiting oral pathogens. Results showed that BS from Bacillus altitudinis were most effective against Candida albicans, while BS from Bacillus siamensis were most effective against Streptococcus mutans and Enterococcus faecalis. The minimum concentrations of CEO inhibiting C. albicans were 1900 ppm and both S. mutans and E. faecalis were 490 ppm. The minimum NEL and NEH concentration inhibiting C. albicans was 3.125 ppm BS+ 175 ppm CEO and 6.25 ppm BS + 350 ppm CEO, respectively. The minimum NEL concentration inhibiting S. mutans and E. faecalis was 7.82 ppm BS + 120 ppm CEO and 1.95 ppm BS + 120 ppm CEO, while the minimum concentration of both NEH was 3.125 ppm BS+ 175 ppm CEO. These findings suggest that BS combined with CEO in nanoemulsions are effective in inhibiting oral pathogens and can serve as promising alternatives to SLS.