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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Indonesian Journal of Applied Research (IJAR) Jurnal Nukleus Peternakan Jurnal Veteriner dan Biomedis Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Dedi Rahmat Setiadi
Departemen Klinik, Reproduksi, Dan Patologi; Fakultas Kedokteran Hewan, Institut Pertanian Bogor (IPB); Jalan Agatis Kampus IPB Dramaga, Bogor, Jawa Barat, INDONESIA, Kode Pos 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Sonographic Appearance of Abdominal Wall at the Left Flank of Laparotomy Incision Site in Ettawah Grade Does Mokhamad Fakhrul Ulum; Dedi R. Setiadi; Budianto Panjaitan; Muchidin Noordin; . Amrozi
Media Peternakan Vol. 37 No. 3 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (953.862 KB) | DOI: 10.5398/medpet.2014.37.3.151

Abstract

The aim of this study was to describe the sonographic appearance of abdominal wall at the left flank of laparotomy incision site in 11 mated Ettawah grade does. Brightness-mode ultrasound examination by using transducer with frequency of 5.0-6.0 MHz was conducted to grouping the does based on their pregnancy statuses. The incision site of the abdominal wall at left flank laparotomy was transcutaneous-scanned as long as 8 cm vertically. The sonographic appearance of the laparotomy wall thickness showed that in all groups of does were similar and not different statistically. The thickness of oblique external and oblique internal abdominal muscles increased in the pregnant does as compared to non-pregnant does (P<0.05).Key words: laparotomy, left flank, ultrasonography, incision site, Ettawah grade does
Karakteristik Sitologi Vagina Selama Siklus Estrus dan Gejala Klinis Estrus pada Banteng (Bos javanicus d’Alton 1823) Dedi Rahmat Setiadi; Muhammad Agil; R Iis Arifiantini; Dondin Sajuthi; Jansen Manansang; Yohana Tri Hastuti; Setyaningsih Rambu Liwa
Acta VETERINARIA Indonesiana Vol. 8 No. 3 (2020): November 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.8.3.40-47

Abstract

Tujuan dari penelitian ini adalah mempelajari karakteristik gambaran sel-sel ephitelial vagina dan gejala klinis estrus pada banteng. Sebanyak 2 ekor banteng betina yang sehat, sudah dewasa kelamin dan pernah beranak diobservasi secara klinis selama siklus estrus normal dan dilakukan pengambilan sampel ulas vagina. Observasi gejala klinis estrus dan ulas vagina dilakukan tiga kali dalam seminggu dan 5 hari berturut-turut menjelang sampai sesudah estrus. Pemeriksaan fisik ini terdiri atas pemeriksaan simptomatis dengan melihat gejala-gejala yang timbul dari luar seperti gelisah, adanya kebengkakkan, kemerahan, kebasahan pada vulva, naik menaiki, melenguh (meskipun pakan cukup tersedia), keluar lendir jernih transparan. Tingkah laku ketika banteng sedang estrus memperlihatkan perilaku saling naik menaiki sesama banteng dan diam ketika dinaiki, sedangkan tanda klinis lainnya seperti kemerahan, kebengkakan, dan lendir serviks tidak terlihat dengan jelas. Sel-sel ephitel yang diperoleh adalah parabasal, intermediet, superfisial dan kornifikasi. Sel superfisial mendominasi (50,20%) pada saat estrus diikuti sel kornifikasi (38,51%), sel intermediet (26,82%) dan sel parabasal 13,44% selama 3 siklus estrus. Hasil penelitian ini dapat disimpulkan bahwa naik-menaiki sesama banteng pada saat estrus merupakan tanda klinis yang paling jelas terlihat dan teknik sitologi vagina dapat digunakan dalam penentuan siklus estrus banteng.
FA-4 Assessment of Estrous Cycle Using Ultrasound to Determine Time of Insemination In Banteng (Bos javanicus, d’Alton 1823) Dedi Rahmat Setiadi; Muhammad Agil; Iis Afriantini; Tuty L Yusuf; Muchidin Noordin; Yohana Tri Hastuti; Setyaningsih Rambu Liwa; Jansen Manansang; Dondin Sajuthi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (592.045 KB)

Abstract

Banteng is considered as endangered species by the International Union for Conservation of Nature (IUCN). Banteng is currently distributed in Java, in Kalimantan [Indonesian Borneo], Sabah [part of Malaysian Borneo], Myanmar, Thailand, Cambodia and, probably Lao PDR and Vietnam (1). The populations in the Asian mainland have decreased by about 80% in the last decades. The total number of wild banteng is estimated to about 5,000-8,000 animals. No population has more than 500 animals, only a few have more than 50. Reasons for the population decline are reduction of habitat, poaching, hybridisation with domesticated cattle, and infections with cattle diseases (4).Since the wild population has been declining continously and the numbers of individual are getting smaller, therefore captive breeding is urgently needed in order to support the conservation of Banteng sustainably. Although breeding program of Banteng is important, but information about the reproductive biology of Banteng is very limited. Breeding programs in Banteng can be done by natural mating or using assisted reproductive technology such as artificial insemination (AI). The aplication of AI in Banteng can support Banteng conservation program in order to increase heterozygosity, prevent inbreeding and possible to use of the Banteng bulls from different facilities for AI of females in all breeding facilities of Banteng.AI applications require information on the estrous cycle and estrous signs to determine the precise AI time. Clinical signs of estrous in Banteng is difficult to observe, therefore determining the time of AI must be done through rectal palpation and ultrasonography. The objectives of  this research is to assess estrous cycle to determine time of insemination.
FA-5 Semen Characteristics of Banteng (Bos javanicus) Collected by Electroejaculation Method Vincentia Trisna Yoelinda; Iis Afriantini; Muhammad Agil; Dedi R Setiadi; Tuty L Yusuf; Yohana Tri Hastuti; Jansen Manansang; Dondin Sajuthi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.227 KB)

Abstract

Banteng (Bos javanicus) is listed as endangered species in IUCN Redlist. Only few thousand wild banteng survive and their numbers are decreasing as a result of hunting and the loss of habitat due to deforestation and conversion to agricultural land. One of the conservation measures is to preserve this species in ex-situ breeding center. The breeding centre with the largest number of individuals is Ragunan Zoo in Jakarta, with 21 animals. Despite those numbers of individuals available, the sex ratio was far from ideal at 16 males and five females. While in Taman Safari Prigen, there were with 16 individuals and a sex ratio of five males and 11 females [1] Since number of males are usually less than females, and also to avoid inbreeding in order to keep high heterozygosity population, therefore, assisted reproductive technology such as artificial insemination is considered to be necessary.For artificial insemination, semen collection sufficient quantitiy and quality of semen from males are needed. Thus, best semen collection method should be considered. Though transrectal massage procedure does not require the male to be anesthetized  prior to semen collection, semen collected by this technique contains urine contamination. Besides, volume of semen sample obtained by transrectal massage is lesser compared to electroejaculation. Electroejaculation is also suitable for wild animal because in this particular semen collection procedure, males are not required to be trained.The information about semen characteristics of banteng bulls collected by electroejaculation is less known. To date, study done by Johnston et al. (2002) is the only available data. That study only provided data from one semen sample from one 4-year-old banteng bull located at Western Plains Zoo [2]. Moreover such volume parameter and consistency was not reported from that study. Thus, this study is aimed to provide more reliable data from larger number of semen samples  collected by electroejaculation and to add information to previous data that are availabe.
Identifikasi Mikroba dari Lendir Estrus dan Deteksi Kebuntingan Sapi Kawin Berulang di Sleman, Yogyakarta Surya Agus Prihatno; Abdul Samik; Dea Indriani Astuti; Muhammad Agil; Usamah Afiff; Anriansyah Renggaman; Dedi Rahmat Setiadi; Yosua Kristian Adi
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70916

Abstract

Repeat breeding is condition of cows that have normal or near normal estrous cycles but fail to become pregnant after several insemination. In this study, we investigated the microbes in the cervical and vaginal mucus of beef cattle and dairy cows that experience repeat breeding and detected the pregnancy after the third or more artificial insemination. A total of 14 beef cattle and 6 dairy cows that experience repeat breeding in the cattle herd in the Yogyakarta region of Indonesia were used as samples in this study. Cervical and vaginal mucus samples were collected using plastic sheet when the cow was re-estrus. The samples were put into the 5 ml Brain Heart Infusion Broth for bacterial isolation and identification. Pregnancy examination was carried out on day 45 after artificial insemination using ultrasound method. Bacteria that could be isolated and identified from cervical mucus and vaginal mucus of beef cattle and dairy cows included Bacillus sp., Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas sp. Some cows detected positive in pregnancy examination even though the bacteria were presence in the cervical and/or vaginal mucus. There was various composition of bacteria found in the cervical mucus and vaginal mucus, of beef cattle and dairy cows with repeat breeding in livestock groups in Yogyakarta. The presence of bacterial in the cervical and vaginal mucus during estrus was not always become the causes of failed pregnancy.
Validasi Kit Enzyme-Linked Immunosorbent Assay Komersial untuk Analisis Hormon Estradiol dan Progesteron Darah Kambing Kacang (VALIDATION OF COMMERCIAL ENZYME-LINKED IMMUNOSORBENT ASSAYKIT FORANALYSIS OF ESTRADIOLANDPROGESTERONE HORMONE IN BLOOD OF KACANG Dedi Rahmat Setiadi; Iman Supriatna; Muhammad Agil
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.061 KB)

Abstract

The aim of this study was to evaluate the capability of two human commercial enzyme-linkedimmunosorbent assay (ELISA) kits(DRG International Inc.,Germany and GBC Taiwan) for measuringestradiol (E2) and progesterone (P4) in plasma of kacang goat. Three healthy and non pregnant femalekacang goats aged 2-3 years with regular estrous cycles were used in this study. Blood samples werecollected from the jugular vein using a 21G venoject every two days and it was intensified every day duringthe period before heat. Collected plasma were stored at -20ºC until the analysis. Capability validationwas conducted by measuring accuracy (parallelism test), sensitivity and precision.Parallelism test usingDRG commercial kit showed that sample curvewas parallel with standard curve of E2 and P4. In contrastit was not parallel with standard curve of GBC commercial kit. Sensitivity was measured from the lowesthormones concentration of E2 and P4 at 90% binding that were 25 pg/ml and 0.14ng/mL in DRG kit, whilein GBC kit were 5 pg/mL of E2 and 0.2 ng/mL of P4, respectively. Coefisien of variation of intra- andinterassay for both ELISA commercial kits were less than10%. It can be concluded that DRG commercialELISA kit E2 and P4 can be used to analyse female kacang goat blood plasma, while GBC commercialELISA kit E2 and P4 are not recommended.
KUALITAS SEMEN DOMBA LOKAL DARI FREKUENSI EJAKULASI BERBEDA (The quality of local ram semen from different ejaculation) Dedi R. Setiadi; Fatimah Fatimah; Didid Diapari; Raden I. Arifiantini
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.6596

Abstract

One of the factors that support the success of reproduction in sheep is the quality of the semen. This study aims to analyze the effect of ejaculation frequency on the quality of ram semen. This study used four local rams two years old. All rams have collected the semen using an artificial vagina five times a day with three replications. The experimental parameters were macroscopic (volume, pH, consistency, and color) and microscopic (mass movement, motility, viability, concentration, normal sperm morphology, and sperm intact plasma membrane). The data analysis used a randomized block design (RBD) with SPSS version 20. The results showed that the frequency of ejaculation in rams shows color and consistency in the 1st ejaculate, which was creamier and thicker than the 4th and 5th ejaculate. Mass movement, sperm concentration per mL, and total sperm concentration in ejaculate 1st and 2nd were higher than in 5th ejaculate. The potential of artificial insemination (AI) in the 1st and 2nd ejaculate was higher than in the 5th ejaculate. The study concluded that the frequency of ejaculation affects mass movement, sperm concentration, and the potential straw production for AI in local sheep.
Pengaruh Penambahan Tepung Protein Maggot Black Soldier Fly (Hermetia illucens) terhadap Palatabilitas Pakan Kucing Komersial Bayu Febram Prasetyo; Dedi Rahmat Setiadi; Handri Dwi Agung
Jurnal Sain Veteriner Vol 41, No 2 (2023): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.81730

Abstract

Larva Black Soldier Fly (BSF) spesies Hermetia illucens mengandung nutrisi protein yang berpotensi menjadi sumber pakan alternatif hewan peliharaan yang ramah lingkungan, berkelanjutan, dan memiliki komposisi nutrisi yang ideal. Namun, laporan penggunaannya masih belum diketahui sebagai bahan pakan kucing. Uji palatabilitas pakan komersial dengan penambahan tepung protein maggot bertujuan untuk mengetahui daya terima pakan yang dikonsumsi. Uji palatabilitas dilakukan dengan metode monadik atau single bowl pada kucing domestik jantan dalam dua tahap, adaptasi pakan dan pemberian pakan uji pada empat kelompok perlakuan (0,25%, 50%, dan 75%) dengan penambahan tepung maggot, yang masing-masing terdiri dari 3 ekor kucing. Hasil pengujian diperoleh dari jumlah konsumsi pakan harian dengan cara jumlah pakan yang diberikan dikurangi dengan jumlah sisa pakan. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan yang nyata (P>0,05) pada perlakuan P1 119±15,36 (g/hari) dan 204±26,31 (kkal/hari) pada jumlah konsumsi pakan dan jumlah kebutuhan kucing harian. Dapat disimpulkan bahwa tepung protein maggot BSF dapat menjadi sumber bahan pakan kucing.
Karakteristik Morfologi Hati Ayam Cemani (Gallus gallus domesticus) Putri Syifa Camilla; Nurhidayat; Heru Setijanto; Supratikno; Chairun Nisa; Srihadi Agungpriyono; Danang Dwi Cahyadi; Dedi Rahmat Setiadi; Savitri Novelina
Jurnal Veteriner dan Biomedis Vol. 1 No. 2 (2023): September
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.1.2.77-83.

Abstract

Tujuan penelitian ini adalah mempelajari morfologi hati ayam cemani (Gallus gallus domesticus) secara makroanatomi dan mikroanatomi. Penelitian ini menggunakan organ hati dari tiga ekor ayam cemani betina. Pengamatan makroanatomi untuk mempelajari morfometri yang meliputi panjang, lebar, tebal, dan berat organ hati. Pengamatan mikroanatomi dilakukan dengan menggunakan pewarnaan Haematoxylin-Eosin, untuk mengamati morfologi sel hati. Data yang diperoleh dianalisis secara deskriptif. Hasil dari pengamatan makroanatomi menunjukkan warna hati adalah merah kecoklatan dengan rata – rata bobot hati sebesar 19.3±2.5 gram. Pengamatan mikroanatomi menunjukkan hati diselaputi oleh jaringan ikat longgar pada permukaannya, kemudian terdapat kapsula Glisson. Di setiap lobulus hati terdapat vena centralis, cabang dari vena porta hepatica, cabang dari arteri hepatica, dan ductus choledochus. Parenkim hati terdiri dari hepatosit dan sinusoid. Sel – sel non parenkim yang terdapat di hati adalah sel Kupffer dan sel endotel. Sel pigmen melanin pada parenkim hati ditemukan dalam jumlah yang sedikit, sebagian besar pigmen melanin terdapat di sekitar vena porta, arteri hepatica, dan ductus choledochus.
EFFECTIVENESS OF VARIOUS COMMERCIAL EXTENDERS IN CRYOPRESERVING SEMEN OF LOCAL INDONESIAN RAMS Setiadi, Dedi Rahmat; Magfira; Riwu, Ronny Markus Jami; Arifiantini, R Iis
Jurnal Bioteknologi dan Biosains Indonesia Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Indonesian local sheep represent a particular species of sheep reared by farmers on small-scale farms. Semen freezing can be performed using either a homemade or commercial extender. The present study evaluated the effectiveness of several commercial extenders in freezing Indonesian local sheep. Semen was collected from three mature and healthy rams using an artificial vagina and evaluated. It was determined that only samples exhibiting sperm motility levels of greater than 70% would be included in the study. Subsequently, the semen from each ram was divided into three portions and diluted with Andromed, Sterydil, and OptiXcell, respectively. The frozen semen was then evaluated 24 hours post-freezing. The thawing process was conducted individually at 37°C for 30 seconds. The evaluation encompassed a range of metrics, including sperm motility, viability, and the presence of any abnormalities, in addition to the recovery rate. The results indicated that sperm frozen in Optixcell and Sterydil extenders exhibited superior motility, viability, and recovery rate than Andromed. The study also demonstrated no interaction between individual Ram and extender, and no differences were found between individuals. The study concluded that Optixcell and Sterydil extenders effectively froze Indonesian local sheep semen.
Co-Authors Abdul Samik Afiff , Usamah Amani, Aqila Zata Amrozi Anriansyah Renggaman Bayu Febram Prasetyo Budianto Panjaitan Chairun Nisa Danang Dwi Cahyadi Dea Indriani Astuti Didid Diapari Dondin Sajuthi Fatimah Fatimah Handri Dwi Agung Hastuti, Yohana Tri Heru Setijanto Iis Afriantini Iman Supriatna Jansen Manansang Jansen Manansang Laksono, Mohamad Nanang Tejo Magfira Manansang, Willem Mokhamad Fakhrul Ulum, Mokhamad Muchidin Noordin Muchidin Noordin MUHAMMAD AGIL Mulia, Bongot Huaso Nurhidayat Putri Syifa Camilla R. Iis Arifiantini Raden I. Arifiantini Riwu, Ronny Markus Jami Savitri Novelina Setyaningsih Rambu Liwa Setyaningsih Rambu Liwa Srihadi Agungpriyono Supratikno . Surya Agus Prihatno Tuty L Yusuf Vincentia Trisna Yoelinda Yohana Tri Hastuti Yohana Tri Hastuti Yosua Kristian Adi