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All Journal Medica Hospitalia Jurnal Material Kedokteran Gigi Acta Odontologica Indonesia
Purnomo, Brigitta Natania Renata
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Dentistry Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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DAYA ANTIBAKTERI EKSTRAK ETANOL KEMBANG SEPATU (Hibiscus rosa sinensis L.) SEBAGAI ALTERNATIF BAHAN MEDIKAMEN SALURAN AKAR TERHADAP Enterococcus faecalis Choirunnisa, Efika; Wibowo, Gustantyo Wahyu; Antari, Arlita Leniseptaria; Purnomo, Brigitta Natania Renata
Medica Hospitalia : Journal of Clinical Medicine Vol. 10 No. 1 (2023): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36408/mhjcm.v10i1.809

Abstract

BACKGROUND: Enterococcus faecalis is the dominant bacteria found in root canals, especially in failure of root canal treatment. Therefore, antibacterial agents are required as a means to achieve complete disinfection of the root canal system. One of the natural ingredients that has been proven to have antibacterial properties is the ethanol extract of hibiscus (H. rosa sinensis L.), so it has the potential to be used as a root canal medicament to inhibit growth and eliminate E. faecalis bacteria.   OBJECTIVE: Testing the effectiveness of hibiscus (H. rosa sinensis L.) ethanol extract as a potential root canal medicament. METHOD: The study was conducted using a true experimental post-test only control group design in 6 treatment groups with various concentrations of hibiscus extract (100%, 50%, 25%, 12.5%, 6.25, and 3.125%) and a positive control group. (Brain Heart Infusion added E. faecalis) and negative (Brain Heart Infusion). The extract was then diluted to the concentration used and added with 1.5x108CFU/ml E. faecalis and then cultured using the spread method on NA media, incubated anaerobically at 37oC for 24 hours, then the colonies that grew were counted using a colony counter. RESULT: The results showed that 100% concentration had the highest ability to inhibit the growth of E. faecalis bacteria (92.7%) with an average number of colonies growing of 4.4 x 102 CFU/ml. The minimum inhibitory concentration (MIC) was shown at a concentration of 50%, with an inhibiting ability of 90.17% and the average number of colonies that grew was 5.9 x103 CFU/ml. Kruskal Wallis statistical test showed a significant difference in the number of colonies (p<0.05). CONCLUSION: Hibiscus extract with a concentration of 100% has the highest ability to inhibit the growth of E. faecalis bacteria and MIC is present at a concentration of 50%.
Pengaruh Aplikasi Gel Nanas (Ananas comosus) Terhadap Kekerasan Email Gigi Sistianingsih, Sistianingsih; Setyadi, Ariosta; Purnomo, Brigitta Natania Renata; Prihatiningsih, Tyas
Jurnal Material Kedokteran Gigi Vol 11 No 1 (2022): JMKG Vol 11 No 1 Maret 2022
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v11i1.713

Abstract

Tooth discoloration is one of the aesthetic problems that have a significant impact on psychological conditions. Pineapple is a natural ingredient that can be used as an alternative to dental bleaching. Citric acid and malic acid in pineapple can bind calcium in teeth and cause porosity of enamel crystals which in turn reduces the enamel hardness. The aim of this study is to determine the effect of pineapple gel (Ananas comosus) application on tooth enamel hardness. The study design was a pretest and post-test controlled group design. Twenty five post extraction maxillary premolars were divided into 5 groups. The pH test of pineapple gel was done before treatment, each group was applied pineapple gel 25%, 50%, 75%, 35% hydrogen peroxide gel, and gel without active compounds. The hardness test was carried out using Vickers Hardness Tester. Data analysis used Paired t-test, Wilcoxon, Kruskal Wallis, one way ANOVA using probability 0,05. Paired t-test results showed significant differences in tooth enamel hardness before and after application of pineapple gel 50% (0,04), 75% (0,00), 35% hydrogen peroxide gel (0,04), but no significant difference in tooth enamel hardness before and after application of 25% pineapple gel (0,16). Wilcoxon test results no significant difference in tooth enamel hardness before and after application of gel without active compounds (0,08). Kruskal Wallis (0,17) and one way ANOVA (0,06) test results no significant differences in tooth enamel hardness between groups. The results of this study concluded that pineapple gel application can be recommended as an alternative to dental bleaching.
Pengaruh Penyemprotan Larutan Asam Hipoklorit (HOCl) dengan Konsentrasi dan Durasi Berbeda terhadap Stabilitas Dimensi Hasil Cetakan Alginat Prajogo, Johanna Kezia; Fulyani, Faizah; Purnomo, Brigitta Natania Renata; Santoso, Oedijani
Acta Odontologica Indonesia Vol 1, No 1 (2025): June Edition
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/actodont.25989

Abstract

Background: Disinfection of alginate impressions is performed to prevent cross-infection. Alginate possesses properties of imbibition and syneresis which affect the dimensional stability of the impression. The disinfectant solution, hypochlorous acid (HOCl), is non-toxic, non-irritating, and non-corrosive at the appropriate concentrations. Hypochlorous acid has higher antimicrobial activity than sodium hypochlorite (NaOCl). Objective: This study aimed to determine the effect of spraying 0.01% and 0.02% HOCl with contact durations of 1 and 5 minutes on the dimensional stability of alginate impressions. Methods: This was a laboratory experimental study with a Post-Test Only Control Group Design. A total of 24 samples were divided into 4 treatment groups and 2 control groups. The dimensional stability of the alginate impressions was assessed by the difference in anteroposterior and mediolateral dimension measurements between the master model and the resulting gypsum casts, using a digital caliper. Measurements were taken 3 times by 3 different observers, followed by a two-way ANOVA statistical test. Results: The results of the two-way ANOVA test showed no significant difference between the groups with variations in concentration or contact duration. The largest dimensional difference was found in the control group sprayed with 5.25% NaOCl. Conclusion: Spraying alginate impressions with 0.01% and 0.02% HOCl with contact durations of 1 and 5 minutes does not affect the dimensional stability of the alginate impressions.
Pengaruh Komposisi Jenis Biji Kopi terhadap Keparahan Diskolorasi Gigi Janto, Matthew Kennard Hery; Purnomo, Brigitta Natania Renata; Hardini, Nadia; Batubara, Lusiana
Acta Odontologica Indonesia Vol 1, No 1 (2025): June Edition
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/actodont.26957

Abstract

Coffee can cause discoloration in the hard tissues of teeth due to the presence of chlorogenic acid, which acts as a staining agent. Different types of coffee bean compositions have varying levels of staining agents. This study aims to investigate the influence of coffee bean composition (100% Arabica, 70%-30% Arabica-Robusta blend, 50%-50% Arabica-Robusta blend, and 100% Robusta) on the severity of tooth discoloration. This research is a quasi-experimental study with a pre-test and post-test control group design to assess the effect of coffee immersion on tooth color change (ΔE). The study involved 24 human premolar tooth samples divided into four treatment groups. Before treatment, the color of the samples was measured (pre-test data), and then the samples were immersed in a coffee solution for 48 hours. After immersion, the color of the samples was measured again (post-test data). Paired t-tests were conducted to determine the significance of differences between pre-test and post-test treatment. Subsequently, Kruskal-Wallis tests were performed to assess the significance of differences among the four treatment groups. Paired t-tests for all groups showed significant results (p < 0.05). Kruskal-Wallis tests indicated a significant difference among the four treatment groups (p < 0.05), with the highest tooth color change (ΔE) observed in the 100% Robusta coffee treatment group and the lowest color change in the 100% Arabica coffee treatment group. The composition of coffee bean types has an impact on the severity of tooth discoloration.
Co-Authors Antari, Arlita Leniseptaria Choirunnisa, Efika Fulyani, Faizah Hardini, Nadia Janto, Matthew Kennard Hery Lusiana Batubara Oedijani Santoso Prajogo, Johanna Kezia Prihatiningsih, Tyas Setyadi, Ariosta Sistianingsih, Sistianingsih Wibowo, Gustantyo Wahyu