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All Journal Journal of Indonesian Dental Association Medica Hospitalia
Wibowo, Gustantyo Wahyu
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Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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DAYA ANTIBAKTERI EKSTRAK ETANOL KEMBANG SEPATU (Hibiscus rosa sinensis L.) SEBAGAI ALTERNATIF BAHAN MEDIKAMEN SALURAN AKAR TERHADAP Enterococcus faecalis Choirunnisa, Efika; Wibowo, Gustantyo Wahyu; Antari, Arlita Leniseptaria; Purnomo, Brigitta Natania Renata
Medica Hospitalia : Journal of Clinical Medicine Vol. 10 No. 1 (2023): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36408/mhjcm.v10i1.809

Abstract

BACKGROUND: Enterococcus faecalis is the dominant bacteria found in root canals, especially in failure of root canal treatment. Therefore, antibacterial agents are required as a means to achieve complete disinfection of the root canal system. One of the natural ingredients that has been proven to have antibacterial properties is the ethanol extract of hibiscus (H. rosa sinensis L.), so it has the potential to be used as a root canal medicament to inhibit growth and eliminate E. faecalis bacteria.   OBJECTIVE: Testing the effectiveness of hibiscus (H. rosa sinensis L.) ethanol extract as a potential root canal medicament. METHOD: The study was conducted using a true experimental post-test only control group design in 6 treatment groups with various concentrations of hibiscus extract (100%, 50%, 25%, 12.5%, 6.25, and 3.125%) and a positive control group. (Brain Heart Infusion added E. faecalis) and negative (Brain Heart Infusion). The extract was then diluted to the concentration used and added with 1.5x108CFU/ml E. faecalis and then cultured using the spread method on NA media, incubated anaerobically at 37oC for 24 hours, then the colonies that grew were counted using a colony counter. RESULT: The results showed that 100% concentration had the highest ability to inhibit the growth of E. faecalis bacteria (92.7%) with an average number of colonies growing of 4.4 x 102 CFU/ml. The minimum inhibitory concentration (MIC) was shown at a concentration of 50%, with an inhibiting ability of 90.17% and the average number of colonies that grew was 5.9 x103 CFU/ml. Kruskal Wallis statistical test showed a significant difference in the number of colonies (p<0.05). CONCLUSION: Hibiscus extract with a concentration of 100% has the highest ability to inhibit the growth of E. faecalis bacteria and MIC is present at a concentration of 50%.
The Effect of 1% Povidone Iodine Mouthwash on The Surface Roughness of Zirconia Reinforced Glass Ionomer Cement Farida, Hifdiyati; Wibowo, Gustantyo Wahyu; Fortuna, Gloria; Nosartika, Isniya
Journal of Indonesian Dental Association Vol 6 No 1 (2023): April
Publisher : Indonesian Dental Association

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Abstract

Introduction: Zirconia reinforced glass ionomer cement is a new generation glass ionomer cement restoration material with the addition of zirconia nanoparticles that is equivalent in strength to amalgam while maintaining ability to release fluoride like conventional glass ionomer cements. Surface roughness can be affected by various factors including the use of mouthwash. Mouthwash that is commonly used during pandemic COVID-19 is 1% povidone iodine. Previous study stated that povidone iodine mouthwash contains alcohol and acidic pH which can decrease the hardness of composite resins. Objective: To determine the effect of 1% povidone iodine mouthwash on the surface roughness of zirconia reinforced glass ionomer cement. Methods: This was a true experimental laboratory research with a pre test and post test control group design. The research samples consisted of 36 samples of zirconia reinforced glass ionomer cement with diameter of 10 mm and height of 2 mm. The samples were divided into two groups: the control group which was immersed in artificial saliva and the treatment group which was immersed in 1% povidone iodine mouthwash for 24 hours. Surface roughness was measured using a surface roughness tester. Data were analyzed using Paired Sample T-Test and Independent Sample T-Test. Results: Paired Sample T-test results showed no significant difference in the surface roughness of the control group between before and after immersion with p value = 0,074 (p>0,05) and a significant difference in the surface roughness of the treatment group between before and after immersion with p value = 0,000 (p<0,05). Independent Sample T-Test results showed significant difference in the surface roughness between the control group and the treatment group with p value = 0,000 (p <0.05). Conclusion: 1% povidone iodine mouthwash has an effect on increasing the surface roughness of zirconia reinforced glass ionomer cement.
Co-Authors Antari, Arlita Leniseptaria Choirunnisa, Efika Farida, Hifdiyati Fortuna, Gloria Nosartika, Isniya Purnomo, Brigitta Natania Renata