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PERBEDAAN POTENSI ANTIBAKTERI BAWANG PUTIH TUNGGAL DENGAN BAWANG PUTIH MAJEMUK TERHADAP SALMONELLA TYPHI Adhuri, Iesha Kinanthi; Kristina, Tri Nur; Antari, Arlita Leniseptaria
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 7, No 2 (2018): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.209 KB) | DOI: 10.14710/dmj.v7i2.20670

Abstract

Latar Belakang: Salmonella typhi, bakteri patogen yang dapat menimbulkan demam tifoid pada manusia, pernah menunjukkan resistensi terhadap antibiotika yang digunakan sebagai first line drugs seperti kloramfenikol, kortimoksazol, tetrasiklin, dan ampisilin.  Sehubungan dengan besarnya masalah resistensi obat, beberapa peneliti telah memilih untuk mengembangkan strategi alternatif. Salah satunya adalah pemanfaatan bawang putih yang mempunyai efek antimikroba. Sebagai obat, belakangan ini, masyarakat mempercayai bawang putih tunggal lebih berkhasiat daripada bawang putih majemuk.Tujuan: Membandingkan potensi anteribakteri bawang putih tunggal dan majemuk terhadap S. typhi.Metode: Metode penelitian ini adalah eksperimental laboratoris. Uji aktivitas antibakteri bawang putih tunggal dan majemuk dengan pelarut etanol 96% dan aquades dilakukan dengan metode difusi dan dilusi.Hasil: Dengan metode difusi, bawang putih tunggal dengan pelarut etanol 96% maupun aquades lebih unggul dibandingkan dengan bawang putih majemuk. Pada metode dilusi, dengan pelarut aquades, bawang putih tunggal juga lebih unggul dibandingkan dengan bawang putih majemuk.  Namun demikian, pada satu pengulangan metode dilusi dengan pelarut etanol 96%, bawang putih majemuk lebih unggul dibandingkan bawang putih tunggal.Simpulan: Bawang putih tunggal memiliki kecenderungan lebih unggul dibandingkan dengan bawang putih majemuk sebagai agen antibakteri terhadap S. typhi.
PREVALENCE AND DEGREE OF SEVERITY OF ACNE VULGARIS IN STUDENTS OF MECHANICAL ENGINEERING MAJOR IN FACULTY OF ENGINEERING DIPONEGORO UNIVERSITY Saragih, Yola Valentin; Widyawati, Widyawati; Utami, Aras; Antari, Arlita Leniseptaria
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 8, No 4 (2019): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.565 KB) | DOI: 10.14710/dmj.v8i4.25787

Abstract

Introduction : Acne vulgaris (AV) is a chronic inflammatory disorder of pilosebaceous follicle with polymorphic dermatological manifestation such as comedones, papules, pustules, nodules, and scar. Acne vulgaris can be categorized into three degrees of severity based on lesions count: mild, moderate, and severe. There are four factors in the pathogenesis of AV: pilosebaceous follicle hyperproliferation, excessive sebum production, inflammation, and presence of Propionibacterium acnes. The degree of severity is mainly related to the number of an individual's sebaceous gland. Individuals with AV usually have large sebaceous glands that are very active, large pores, and oily skin. Objectives : To describe prevalence and severity of acne vulgaris in students of mechanical engineering major in faculty of engineering Diponegoro University batch 2018. Methods : This is an observational study with cross sectional approach in which author describes prevalence of AV and its degree of severity in students of mechanical engineering major in faculty of engineering diponegoro university batch 2018. Subjetcs were obtained from simple random sampling. There were 71 eligible subjects meeting inclusion criterias. Dermatovenereology residents performed physical examination on subjects to determine whether or not the subject had. Subjects with AV were classified into "positive" group, otherwise "negative". Subjects were obtained with simple random sampling technique. All subjects (100%) had acne vulgaris. Severity of AV varies with 38% subjects had mild AV, 59.2% had moderate AV and 2.8% had severe AV.Conclusions : All subjects (100%) had AV, mostly of moderate severity. Keywords : Acne Vulgaris; prevalence; severity
ANTIMICROBIAL EFFECT OF ARROWROOT (Maranta arundinacea L.) METHANOLIC EXTRACT AGAINST Staphylococcus aureus BACTERIAL GROWTH Syahputra, Muhammad Ghaza; Antari, Arlita Leniseptaria; Winarto, Winarto; Lestari, Endang Sri
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 9, No 3 (2020): DIPONEGORO MEDICAL JOURNAL ( Jurnal Kedokteran Diponegoro )
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.863 KB) | DOI: 10.14710/dmj.v9i3.27499

Abstract

Introduction: Staphylococcus aureus is a spherical gram-positive bacterium that forms clusters and is frequently resistant to antibiotics. Methicillin-Resistant Staphylococcus aureus (MRSA) is a strain of S. aureus that is resistant to beta-lactam and cephalosporin groups of antibiotics, it is one of the biggest problems in resistant bacteria. Plants that have flavonoids in their extract can inhibit the growth of bacteria by impairing their cytoplasmic membrane. Arrowroot is an example of plants that have flavonoids in their extracts. Objectives: This research aims to determine the antimicrobial effect of arrowroot (Maranta arundinacea L.) methanolic extract against the growth of Staphylococcus aureus in vitro. Methods: This is true experimental research with a post-test control group design with two control groups and eight experimental groups. The sample of this research utilizes the MRSA strain of S. aureus received from the Microbiology Laboratory of Diponegoro University Faculty of Medicine that was eligible with the inclusion criteria. The research was conducted by using arrowroot extract concentrations 6.5%, 12.5%, 25%, 50%, and 100% to determine the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and inhibitory zone diameter. Results: MIC and MBC of arrowroot methanolic extract against MRSA is 100%. The arrowroot methanolic extract to have yielded an inhibitory zone diameter is 100% with a mean inhibitory zone diameter of 15.5mm. Conclusion: Arrowroot methanolic extract can inhibit the bacterial growth of MRSAKeywords: Staphylococcus aureus, growth,  M. arundinacea L. methanolic extract
Nephrolepis exaltata - Hibiscus rosa-sinensis combination herbal mask decreases levels of reactive oxygen species in nasal wash Prasetyo, Awal; Ariani, Resti; Antari, Arlita Leniseptaria; Sadhana, Udadi; Miranti, Ika Pawitra; Kristina, Tri Nur
Universa Medicina Vol. 39 No. 3 (2020)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2020.v39.184-191

Abstract

BackgroundPoor air and mask quality in the work environment increase the risks of inflammation, reactive oxygen species (ROS) production, ongoing oxidative stress, and superoxide dismutase 3 (SOD3) levels in the airways. The study aimed to investigate the effect of the Nephrolepis exaltata - Hibiscus rosa-sinensis (NE-HRS) combination herbal mask on the airways, as measured by ROS and SOD3 levels in the nasal wash of textile industry workers.MethodsThis was a pre- and post-test randomized controlled trial, consisting of 30 subjects with ³5-year working time, conducted for eight weeks and monitored every ten days. Subjects were randomized into 3 groups, regular surgical mask group (n=10), NE-HRS combination herbal mask group (n=10) and factory mask group (n=10). SOD3 and ROS levels were determined using ELISA. Data were analyzed with One-way ANOVA and multiple comparison tests.Results ROS levels decreased significantly in all groups (p=0.004). Regular surgical masks decreased ROS levels by 67.90 ± 50.66 pg/mL, NE-HRS combination herbal masks by 49.91 ± 45.06 pg/mL, and factory masks by 193.96 ± 128.36 pg/ml. SOD3 levels also decreased but not significantly (p=0.053). Differences in ROS levels between surgical mask and NE-HRS combination herbal mask was not significant (p=0.796). ConclusionHerbal masks laminated with NE-HRS combination significantly reduced ROS levels among textile industry workers. NE-HRS combination herbal masks have a similar protective effect to the regular surgical mask.
The use of herbal medicines in patients with type-2 diabetes mellitus in Indonesia Utomo, Astika Widy; Annisaa, Eva; Antari, Arlita Leniseptaria; Armalina, Desy
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 13, No 1 (2022): June 2022
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (866.994 KB) | DOI: 10.30659/sainsmed.v13i1.13487

Abstract

In Indonesia, the use of herbal medicine as an alternative medicine is increasing. Herbal remedies are used as complementary medicine and self-medication. Diabetes mellitus requires treatment over the long term. This may contribute to the medication adherence problem. Consequently, many patients seek alternative treatments, such as herbal medications. The purpose of this study was to determine the use and type of herbal medicines among diabetic patients in Indonesia. A cross-sectional study with 190 respondents selected at random from multiple cities in Indonesia. A questionnaire was used to determine the most frequently used herbal medicine, the demographic profile of the patients, the health services, and the level of patient satisfaction with the use of herbal medicines. The participants were predominantly female (62.7%), housewives (39.5%), aged between 40 and 65 (84.5%), high school graduates (74.7%), and had low to moderate incomes (74.7%). The use of herbal medicine was 22%, and the combination of herbal medicine and prescription medication was 6%. Bitter leaves (10%), mangosteen peel (8.9%), insulin leaves (7.9%), mahkota dewa herbs (6.3%), and bitter melon are used as herbal or traditional medicines (4.7%). In terms of satisfaction, 28.7%, 40.9%, and 14.4% of respondents were quite satisfied, satisfied, and very satisfied with the use of herbal medicines, respectively. Herbal remedies are commonly used in alternative diabetes treatments. Bitter leaves are the most frequently used herb, with over half of respondents expressing satisfaction with the benefits of herbal medicine.
Healthcarepreneurship Training on Making Antibacterial Liquid Soap (Hand Soap) Made From Hibiscus rosa sinensis L. Extracts Antari, Arlita Leniseptaria; Saraswati, Indah; Annisaa, Eva; Utomo, Astika Widy; Ekawati, Nuraini; Kurniasari, Teny
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 10, No 2 (2024): June
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.89565

Abstract

Healthcare is one of the critical elements of infection prevention and control, one of which can be done by maintaining hand hygiene. Hand washing with soap and flowing water has long been considered a parameter of personal hygiene and is a simple way to reduce the transmission of infections, especially those caused by hand contact between individuals. However, it must still be done by paying attention to the time and the correct way to wash hands according to WHO standards. At this time, various hand soap products have emerged but are not antibacterial. Antibacterial ingredients used as soap base should not contain various kinds of chemicals that are harmful to the health of its users. Therefore, the use of natural ingredients can be an alternative to substitute chemical active substances. One that has been tested for its antibacterial properties is hibiscus flower (Hibiscus rosa sinensis L.). This activity began with presenting the results of the screening on hand washing habits carried out 2 weeks earlier. The next activity is the introduction to making antibacterial hand soap made from hibiscus extract through material presentation and empirical evidence of the potential of hibiscus as an antibacterial, as well as videos about the importance of maintaining hand hygiene through proper hand washing regularly. The activity is expected to make the healthcare UMKM know and understand that hibiscus flowers, which are widely available around their residence, can be used as raw material for making hand soap that has been produced so far, so as to minimize the use of chemical-based antibacterials. The hand soap product produced later became a collaborative product produced by the community, and then registered a patent with the number S00202120213 on November 17th, 2021.
DAYA ANTIBAKTERI EKSTRAK ETANOL KEMBANG SEPATU (Hibiscus rosa sinensis L.) SEBAGAI ALTERNATIF BAHAN MEDIKAMEN SALURAN AKAR TERHADAP Enterococcus faecalis Choirunnisa, Efika; Wibowo, Gustantyo Wahyu; Antari, Arlita Leniseptaria; Purnomo, Brigitta Natania Renata
Medica Hospitalia : Journal of Clinical Medicine Vol. 10 No. 1 (2023): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36408/mhjcm.v10i1.809

Abstract

BACKGROUND: Enterococcus faecalis is the dominant bacteria found in root canals, especially in failure of root canal treatment. Therefore, antibacterial agents are required as a means to achieve complete disinfection of the root canal system. One of the natural ingredients that has been proven to have antibacterial properties is the ethanol extract of hibiscus (H. rosa sinensis L.), so it has the potential to be used as a root canal medicament to inhibit growth and eliminate E. faecalis bacteria.   OBJECTIVE: Testing the effectiveness of hibiscus (H. rosa sinensis L.) ethanol extract as a potential root canal medicament. METHOD: The study was conducted using a true experimental post-test only control group design in 6 treatment groups with various concentrations of hibiscus extract (100%, 50%, 25%, 12.5%, 6.25, and 3.125%) and a positive control group. (Brain Heart Infusion added E. faecalis) and negative (Brain Heart Infusion). The extract was then diluted to the concentration used and added with 1.5x108CFU/ml E. faecalis and then cultured using the spread method on NA media, incubated anaerobically at 37oC for 24 hours, then the colonies that grew were counted using a colony counter. RESULT: The results showed that 100% concentration had the highest ability to inhibit the growth of E. faecalis bacteria (92.7%) with an average number of colonies growing of 4.4 x 102 CFU/ml. The minimum inhibitory concentration (MIC) was shown at a concentration of 50%, with an inhibiting ability of 90.17% and the average number of colonies that grew was 5.9 x103 CFU/ml. Kruskal Wallis statistical test showed a significant difference in the number of colonies (p<0.05). CONCLUSION: Hibiscus extract with a concentration of 100% has the highest ability to inhibit the growth of E. faecalis bacteria and MIC is present at a concentration of 50%.
Formulation and Characterization of Analog Rice Using Arrowroot (Maranta arundinacea L.) and Hibiscus Flower (Hibiscus rosa sinensis L.) Antari, Arlita Leniseptaria; Saraswati, Indah; Eva Annisa; Anfa Adnia Fatma
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 11 No. 3 (2024): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v11i32024.386-394

Abstract

Background: Efforts to reduce high-carbohydrate consumption from rice include the development of analog rice from alternative sources such as arrowroot tubers (Maranta arundinacea L.). Proper formulation is crucial for producing analog rice with optimal quality, taste, and characteristics resembling conventional rice, while maintaining functional properties to meet carbohydrate and nutritional needs. Hibiscus (Hibiscus rosa-sinensis L.), which is rich in flavonoids, saponins, and anthocyanins, can enhance these formulations. Objective: The objective of this study was to evaluate the composition of a blend of arrowroot tubers (M. arundinacea L.) and hibiscus (H. rosa-sinensis L.) as a rice substitute. Methods: This study used 50 untrained panelists to conduct organoleptic tests on the shape, color, aroma, taste, and texture of rice and analog rice using five formulas (F1, F2, F3, F4, and F5). Furthermore, physical characteristics were tested, including the color index, 1000-grain weight, bulk density, and starch digestibility. Results: The characteristics test proved that all analog rice formulas had an average hedonic score in the range of 3.73-3.90; lower bulk Density than rice with a bulk density of 0.83 ± 0.02 g/mL; starch digestibility in the range of 0.62-0.67 g/mL; and has a yellow-red color range. Conclusion: The best and most preferred rice analog is Formula 5 (F5), with a composition of arrowroot tubers 57.8%, hibiscus 5.1%, mocaf 12.6%, GMS 0.6%, and water 23.9%.
Ekstrak Hibiscus rosa-sinensis L. Memperbaiki Kadar Glukosa Darah Puasa dan Jumlah Sel Mast pada Model Tikus Diabetes Terinfeksi Mycobacterium tuberculosis: Hibiscus rosa-sinensis L. Extract Improves Fasting Blood Glucose Levels and Number of Mast Cells in Diabetic Rat Model that Infected with Mycobacterium tuberculosis Pakaya, David; Dewi, Marzela; Antari, Arlita Leniseptaria; Saraswati, Indah
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 6 No. 1 (2020): (March 2020)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.89 KB) | DOI: 10.22487/j24428744.2020.v6.i1.13930

Abstract

Background: The condition of hyperglycemia in Diabetes mellitus (DM) causes a disruption of the function of phagocytes that can facilitate M. tuberculosis infection. Immunostimulant from natural ingredients can help improve the condition of hyperglycemia an immune function. Objective: To analyze the effect of Hibiscus rosa-sinensis L. extract on fasting blood glucose level and the number of mast cell in TB-DM mouse model. Methods: This experimental study used 27 male Wistar rats, 8 weeks of age, 200-230 g weight, divided into 3 groups, K: Normal control group, P1: TB-positive mouse group P2: Group rat TB-DM + Hibiscus rosa-sinensis L extract dose 250 mg/kgBW and 3 time measurement of H + 7 DM, H + 14 DM and H + 21 DM. TB-DM model mice were prepared with the administration of nicotinamide (NA) 100 mg/kgBW and streptozotocin (STZ) 65 mg/Kg BW, TB agent 1.5 x 105 CFU. Fasting blood glucose levels were measured by GOD-PAP method and Pulmonary tissue is excised, made in paraffin blocks and stained with blue Toluidine to count the mast cells. Results: There was an improvement in the mean fasting blood glucose levels in the group treated with the Hibiscus rosa-sinensis L. extract of 250 mg/kgBW compared to the TB-DM group without extract therapy followed by decreased the number of mast cell. Conclusion: Hibiscus rosa-sinensis L. extract. improves fasting blood glucose levels and decreases the number of mast cell of TB-DM rat model.
Biosmart and safe bus : new approach implementing epidemiologic triangle to reduce pathogen transmission on public transportation Mentari, Alfitra Akbar Bara Mentari; Antari, Arlita Leniseptaria; Saraswati, Indah; Firmanti, Stefani Candra; Subagio, Agus; Prasetyo, Awal; Taufiq, Heydar Ruffa; Rowi, Khasan; Gufron, Ahmad
BKM Public Health and Community Medicine The 12th UGM Public Health Symposium
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Indirect pathogen transmission is one of the factors that cause the transmission of infectious problems from human to human. Surface cabine bus is one of the places that probably can be an intermediary of indirect pathogen transmission. Biosmart and Safe Bus is the concept of implementing an epidemiologic triangle, one of the innovations in Biosmart and Safe Bus is implementing silver nanoparticles as a coating in the cabine to decrease the bacterial load on the surface. Objective: To determine the effectiveness of coating silver nanoparticles in decreasing the bacterial surface load on the Biosmart and Safe Bus concept. Methods: This research used a post-test-only design with a control group. The sampling technique in this research is using surface swab sampling with a total of 18 samples in each Biosmart and Safe Bus and conventional bus that turn to Jakarta and Batu from Semarang. The data were analyzed by using the Mann-Whitney test, the Kruskal-Wallis test, and pairwise comparison Kruskal-Wallis test. Results: The results showed a significant difference between bacterial surface load on Biosmart and Safe Bus and conventional bus (p = 0.015). Along with it, the implementation of coating silver nanoparticles has a considerable effect (cohen effect size = 1.0324) in decreasing the bacterial surface load on the cabin of Biosmart and Safe Bus. The use of silver nanoparticles as a coating on the surface of Biosmart and Safe Bus is effective in being a surface antibacterial agent. This is in line with another research conducted in the United Kingdom with the result that the use of Ag antimicrobial BioCote as coating can reduce 95% surface bacteria. Conclusion: This research proved that coating silver nanoparticles is effective in decreasing the bacterial surface load on Biosmart and Safe Bus compared to a conventional bus.